Fingerprint Analysis of Five New Cultivars of Populus deltoides Based on CE-AFLP

JIA Hui-xia; HU Jian-jun; LU Meng-zhu

Volume 26 Issue 3

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Fingerprint Analysis of Five New Cultivars of Populus deltoides Based on CE-AFLP

1.
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing 100091, China

Received Date: 2012-01-16

Abstract

CE-AFLP(Capillary electrophoresis-amplified fragment length polymorphism)was used to construct the fingerprint and analyze the genetic diversity of five new cultivars of Populus deltoides, including Populus deltoides CL. ‘Danhong’, P. deltoides CL. ‘Nan’, P. deltoides CL. ‘Zhonglin2025’, P. deltoides CL. ‘Zhonghong’, and P. deltoides CL. ‘Quanhong’. The primer combinations of EcoR I+3/Mse I+3 and Pst I+2/Mse I+3 were used in this study. The result indicated that 12 pairs of primers could be detected with many stable and clear amplified bands in all the 36 primer pairs. 877 bands were obtained, including 315 polymorphic bands, and the polymorphism frequency was 35.92%. UPGMA cluster analysis showed that the similarity coefficient ranged from 0.74 to 1.00. The difference between ‘Danhong’ and ‘Nan’ was large. The similarity coefficient of ‘Zhonghong’ and ‘Quanhong’ with ‘Zhonglin2025’ was 0.98. 11 stable and clear specific bands could be used to identify these cultivars. There were not detected stable and clear specific bands between ‘Zhonghong’ and ‘Quanhong’. It suggested that the variability of genomic between the two cultivars was not dramatic.
- CE-AFLP,
- Populus deltoides,
- fingerprint,
- genetic diversity

References

[1]	方升佐. 中国杨树人工林培育技术研究进展[J]. 应用生态学报, 2008, 19(10): 2308-2316
[2]	刘喜荣, 安丽, 刘长敏, 等. 廊坊地区黑杨派新无性系引种试验结果初报[J]. 林业实用技术, 2009(7): 23-24
[3]	王会刚, 马鸿鹏, 田新辉, 等. 10 个速生杨品系对比试验研究[J]. 河北林果研究, 2009, 24(4): 357-361
[4]	陈章水. 杨树栽培实用技术[M]. 北京: 中国林业出版社, 2005:115-128
[5]	胡建军, 李淑梅, 卢孟柱, 等. 转Bt基因欧洲黑杨抗虫稳定性及其对天敌昆虫的影响[J]. 林业科学研究, 2007, 20(5): 656-659
[6]	张春玲, 李淑梅, 赵自成, 等. 杨树新品种'丹红杨’[J]. 林业科学, 2008, 44(1): 169
[7]	韩文超. 速生抗虫杨树新品种-巨霸[J]. 农村实用技术与信息, 2005(12):37
[8]	朱延林, 程相军. 杨树新品种-中红杨[J]. 林业科学, 2008, 44(10):173-174
[9]	Vos P, Hogers R, Bleeker M, et al. AFLP: a new technique for DNA fingerprinting[J]. Nucleic acids research, 1995, 23(21): 4407- 4414
[10]	Muluvi G, Sprent J, Soranzo N, et al. Amplified fragment length polymorphism (AFLP) analysis of genetic variation in Moringa oleifera Lam[J]. Molecular Ecology, 1999, 8(3): 463-470
[11]	Sharma S K, Knox M R, Ellis T H N. AFLP analysis of the diversity and phylogeny of Lens and its comparison with RAPD analysis[J]. TAG Theoretical and Applied Genetics, 1996, 93(5): 751-758
[12]	张书芬, 傅廷栋, 马朝芝, 等. 3 种分子标记分析油菜品种间的多态性效率比较[J]. 中国油料作物学报, 2005, 27(2): 19-23
[13]	Vuylsteke M, Peleman J D, Van Eijk M J T. AFLP technology for DNA fingerprinting[J]. Nature protocols, 2007, 2(6): 1387-1398
[14]	尹佟明, 孙晔. 美洲黑杨无性系AFLP 指纹分析[J]. 植物学报:英文版, 1998, 40(8): 778-780
[15]	宋红竹, 张绮纹, 周春江. 杨树部分种的AFLP 遗传多样性分析[J]. 林业科学, 2007, 43(12): 64-69
[16]	李金花, 宋红竹, 周春江, 等. 北方地区黑杨派栽培品种的AFLP 指纹分析[J]. 林业科学研究, 2009, 22(2): 207-212
[17]	李淑娴, 陈志银, 沈永宝, 等. 松属树种种苗鉴定中AFLP指纹技术的研究[J]. 南京林业大学学报:自然科学版, 2003, 27(3): 1-6
[18]	王利, 邢世岩, 王芳, 等. 银杏雌株种质遗传关系的AFLP分析[J]. 林业科学, 2008, 44(4): 48-53
[19]	闵会, 程诗明, 康志雄, 等. 香榧天然群体遗传多样性的AFLP分析[J]. 林业科学研究, 2009, 22(3): 367-372
[20]	Wong K, Chuan Y, Aggarwal A, et al. Identifying patterns of DNA for tumor diagnosis using capillary electrophoresis-amplified fragment length polymorphism (CE-AFLP) screening[J]. Journal of Bioinformatics and Computational Biology, 2004, 2(3): 569-588
[21]	Luo M C, Thomas C, You F M, et al. High-throughput fingerprinting of bacterial artificial chromosomes using the snapshot labeling kit and sizing of restriction fragments by capillary electrophoresis[J]. Genomics, 2003, 82(3): 378-389
[22]	Maguire T, Peakall R, Saenger P. Comparative analysis of genetic diversity in the mangrove species Avicennia marina (Forsk.) Vierh.(Avicenniaceae) detected by AFLPs and SSRs[J]. TAG Theoretical and Applied Genetics, 2002, 104(2): 388-398
[23]	Karaca M, Saha S, Zipf A. Genetic diversity among forage bermudagrass(Cynodon spp.):evidence from chloroplast and nuclear DNA fingerprinting[J]. Crop science,2002,42(6):2118-2127
[24]	季鹏章, 蒋会兵, 黄兴奇, 等. 古茶园, 台地茶园遗传多样性的AFLP分析[J]. 遗传, 2009, 31(1): 101-108
[25]	李凤霞, 王卫锋, 王鲁, 等. 烟草属植物遗传多样性和亲缘进化关系的荧光AFLP分析[J]. 中国农业科学, 2010, 43(12): 2418-2427
[26]	吕金辉, 胡建军, 卢孟柱. 基于毛细管电泳的柳树AFLP分子标记研究[J]. 北京林业大学学报, 2012, 34(1): 51-57
[27]	冯锦霞, 张川红, 郑勇奇, 等. 利用荧光SSR标记鉴别杨树品种[J]. 林业科学, 2011, 47(6): 167-174
[28]	Ma Q, Zhang J, Pei D. Genetic analysis of Walnut cultivars in China using fluorescent amplified fragment length polymorphism[J]. Journal of the American Society for Horticultural Science, 2011, 136(6):422-428
[29]	Braglia L, Bruna S, Lanteri S, et al. An AFLP-based assessment of the genetic diversity within Hibiscus rosa-sinensis and its place within the Hibiscus genus complex[J]. Scientia Horticulturae, 2010, 123(3): 372-378
[30]	程小毛, 岳琳, 黄晓霞. 基于AFLP 标记技术的三翅槭与三角枫亲缘关系分析[J]. 中国农学通报, 2011, 27(16): 79-83
[31]	黄仁志, 颜新培, 李健, 等. 湖南省10个现行栽培桑树品种的AFLP指纹图谱分析[J]. 蚕业科学,2009,35(4):837-841
[32]	Julio E, Verrier J, Dorlhac de Borne F. Development of SCAR markers linked to three disease resistances based on AFLP within Nicotiana tabacum L[J]. TAG Theoretical and Applied Genetics, 2006,112(2): 335-346
[33]	Negi M, Devic M, Delseny M, et al. Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to a SCAR marker for rapid selection[J]. TAG Theoretical and Applied Genetics, 2000, 101(1): 146-152
[34]	Shen A, Li H, Wang K, et al. Sequence characterized amplified region (SCAR) markers-based rapid molecular typing and identification of Cunninghamia lanceolata[J]. African Journal of Biotechnology, 2011,10(82): 19066-19074
[35]	Sarwat M, Nabi G, Das S, et al. Molecular markers in medicinal plant biotechnology: past and present[J]. Critical Reviews in Biotechnology, 2012, 32(1): 74-92
[36]	Miao L, Shou S, Cai J, et al. Identification of two AFLP markers linked to bacterial wilt resistance in tomato and conversion to SCAR markers[J]. Molecular biology reports, 2009, 36(3): 479-486

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Fingerprint Analysis of Five New Cultivars of Populus deltoides Based on CE-AFLP

1. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Beijing 100091, China

Received Date: 2012-01-16

Abstract: CE-AFLP(Capillary electrophoresis-amplified fragment length polymorphism)was used to construct the fingerprint and analyze the genetic diversity of five new cultivars of Populus deltoides, including Populus deltoides CL. ‘Danhong’, P. deltoides CL. ‘Nan’, P. deltoides CL. ‘Zhonglin2025’, P. deltoides CL. ‘Zhonghong’, and P. deltoides CL. ‘Quanhong’. The primer combinations of EcoR I+3/Mse I+3 and Pst I+2/Mse I+3 were used in this study. The result indicated that 12 pairs of primers could be detected with many stable and clear amplified bands in all the 36 primer pairs. 877 bands were obtained, including 315 polymorphic bands, and the polymorphism frequency was 35.92%. UPGMA cluster analysis showed that the similarity coefficient ranged from 0.74 to 1.00. The difference between ‘Danhong’ and ‘Nan’ was large. The similarity coefficient of ‘Zhonghong’ and ‘Quanhong’ with ‘Zhonglin2025’ was 0.98. 11 stable and clear specific bands could be used to identify these cultivars. There were not detected stable and clear specific bands between ‘Zhonghong’ and ‘Quanhong’. It suggested that the variability of genomic between the two cultivars was not dramatic.

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Reference (36)

[1]	方升佐. 中国杨树人工林培育技术研究进展[J]. 应用生态学报, 2008, 19(10): 2308-2316
[2]	刘喜荣, 安丽, 刘长敏, 等. 廊坊地区黑杨派新无性系引种试验结果初报[J]. 林业实用技术, 2009(7): 23-24
[3]	王会刚, 马鸿鹏, 田新辉, 等. 10 个速生杨品系对比试验研究[J]. 河北林果研究, 2009, 24(4): 357-361
[4]	陈章水. 杨树栽培实用技术[M]. 北京: 中国林业出版社, 2005:115-128
[5]	胡建军, 李淑梅, 卢孟柱, 等. 转Bt基因欧洲黑杨抗虫稳定性及其对天敌昆虫的影响[J]. 林业科学研究, 2007, 20(5): 656-659
[6]	张春玲, 李淑梅, 赵自成, 等. 杨树新品种'丹红杨’[J]. 林业科学, 2008, 44(1): 169
[7]	韩文超. 速生抗虫杨树新品种-巨霸[J]. 农村实用技术与信息, 2005(12):37
[8]	朱延林, 程相军. 杨树新品种-中红杨[J]. 林业科学, 2008, 44(10):173-174
[9]	Vos P, Hogers R, Bleeker M, et al. AFLP: a new technique for DNA fingerprinting[J]. Nucleic acids research, 1995, 23(21): 4407- 4414
[10]	Muluvi G, Sprent J, Soranzo N, et al. Amplified fragment length polymorphism (AFLP) analysis of genetic variation in Moringa oleifera Lam[J]. Molecular Ecology, 1999, 8(3): 463-470
[11]	Sharma S K, Knox M R, Ellis T H N. AFLP analysis of the diversity and phylogeny of Lens and its comparison with RAPD analysis[J]. TAG Theoretical and Applied Genetics, 1996, 93(5): 751-758
[12]	张书芬, 傅廷栋, 马朝芝, 等. 3 种分子标记分析油菜品种间的多态性效率比较[J]. 中国油料作物学报, 2005, 27(2): 19-23
[13]	Vuylsteke M, Peleman J D, Van Eijk M J T. AFLP technology for DNA fingerprinting[J]. Nature protocols, 2007, 2(6): 1387-1398
[14]	尹佟明, 孙晔. 美洲黑杨无性系AFLP 指纹分析[J]. 植物学报:英文版, 1998, 40(8): 778-780
[15]	宋红竹, 张绮纹, 周春江. 杨树部分种的AFLP 遗传多样性分析[J]. 林业科学, 2007, 43(12): 64-69
[16]	李金花, 宋红竹, 周春江, 等. 北方地区黑杨派栽培品种的AFLP 指纹分析[J]. 林业科学研究, 2009, 22(2): 207-212
[17]	李淑娴, 陈志银, 沈永宝, 等. 松属树种种苗鉴定中AFLP指纹技术的研究[J]. 南京林业大学学报:自然科学版, 2003, 27(3): 1-6
[18]	王利, 邢世岩, 王芳, 等. 银杏雌株种质遗传关系的AFLP分析[J]. 林业科学, 2008, 44(4): 48-53
[19]	闵会, 程诗明, 康志雄, 等. 香榧天然群体遗传多样性的AFLP分析[J]. 林业科学研究, 2009, 22(3): 367-372
[20]	Wong K, Chuan Y, Aggarwal A, et al. Identifying patterns of DNA for tumor diagnosis using capillary electrophoresis-amplified fragment length polymorphism (CE-AFLP) screening[J]. Journal of Bioinformatics and Computational Biology, 2004, 2(3): 569-588
[21]	Luo M C, Thomas C, You F M, et al. High-throughput fingerprinting of bacterial artificial chromosomes using the snapshot labeling kit and sizing of restriction fragments by capillary electrophoresis[J]. Genomics, 2003, 82(3): 378-389
[22]	Maguire T, Peakall R, Saenger P. Comparative analysis of genetic diversity in the mangrove species Avicennia marina (Forsk.) Vierh.(Avicenniaceae) detected by AFLPs and SSRs[J]. TAG Theoretical and Applied Genetics, 2002, 104(2): 388-398
[23]	Karaca M, Saha S, Zipf A. Genetic diversity among forage bermudagrass(Cynodon spp.):evidence from chloroplast and nuclear DNA fingerprinting[J]. Crop science,2002,42(6):2118-2127
[24]	季鹏章, 蒋会兵, 黄兴奇, 等. 古茶园, 台地茶园遗传多样性的AFLP分析[J]. 遗传, 2009, 31(1): 101-108
[25]	李凤霞, 王卫锋, 王鲁, 等. 烟草属植物遗传多样性和亲缘进化关系的荧光AFLP分析[J]. 中国农业科学, 2010, 43(12): 2418-2427
[26]	吕金辉, 胡建军, 卢孟柱. 基于毛细管电泳的柳树AFLP分子标记研究[J]. 北京林业大学学报, 2012, 34(1): 51-57
[27]	冯锦霞, 张川红, 郑勇奇, 等. 利用荧光SSR标记鉴别杨树品种[J]. 林业科学, 2011, 47(6): 167-174
[28]	Ma Q, Zhang J, Pei D. Genetic analysis of Walnut cultivars in China using fluorescent amplified fragment length polymorphism[J]. Journal of the American Society for Horticultural Science, 2011, 136(6):422-428
[29]	Braglia L, Bruna S, Lanteri S, et al. An AFLP-based assessment of the genetic diversity within Hibiscus rosa-sinensis and its place within the Hibiscus genus complex[J]. Scientia Horticulturae, 2010, 123(3): 372-378
[30]	程小毛, 岳琳, 黄晓霞. 基于AFLP 标记技术的三翅槭与三角枫亲缘关系分析[J]. 中国农学通报, 2011, 27(16): 79-83
[31]	黄仁志, 颜新培, 李健, 等. 湖南省10个现行栽培桑树品种的AFLP指纹图谱分析[J]. 蚕业科学,2009,35(4):837-841
[32]	Julio E, Verrier J, Dorlhac de Borne F. Development of SCAR markers linked to three disease resistances based on AFLP within Nicotiana tabacum L[J]. TAG Theoretical and Applied Genetics, 2006,112(2): 335-346
[33]	Negi M, Devic M, Delseny M, et al. Identification of AFLP fragments linked to seed coat colour in Brassica juncea and conversion to a SCAR marker for rapid selection[J]. TAG Theoretical and Applied Genetics, 2000, 101(1): 146-152
[34]	Shen A, Li H, Wang K, et al. Sequence characterized amplified region (SCAR) markers-based rapid molecular typing and identification of Cunninghamia lanceolata[J]. African Journal of Biotechnology, 2011,10(82): 19066-19074
[35]	Sarwat M, Nabi G, Das S, et al. Molecular markers in medicinal plant biotechnology: past and present[J]. Critical Reviews in Biotechnology, 2012, 32(1): 74-92
[36]	Miao L, Shou S, Cai J, et al. Identification of two AFLP markers linked to bacterial wilt resistance in tomato and conversion to SCAR markers[J]. Molecular biology reports, 2009, 36(3): 479-486

Fingerprint Analysis of Five New Cultivars of Populus deltoides Based on CE-AFLP

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Fingerprint Analysis of Five New Cultivars of Populus deltoides Based on CE-AFLP

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