Predominant Species Dynamic and Diversity of Fungal Endophytes in Barks of Two Populus Cultivars

LI Yong; PIAO Chun-gen; GUO Li-min; CHANG Ju-pu; WANG Hai-ming; HE Wei; XIE Shou-jiang; GUO Min-wei

Volume 26 Issue 3

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Predominant Species Dynamic and Diversity of Fungal Endophytes in Barks of Two Populus Cultivars

1.
Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China
2.
Puyang Academy of Forestry, Puyang 457000, He’nan, China
3.
Heze Forest Protection Station, Heze 274002, Shandong, China
4.
Beijing Forestry University, Beijing 100083, China

Received Date: 2012-12-17

Abstract

In order to understand the predominant species dynamic and diversity of fungal endophytes in barks of Populus×euramericana cv. Robusta 94 and triploid of P. tomentosa,the fungal endophytes were isolated from barks of P.×euramericana cv. Robusta 94 and triploid of P. tomentosa by tissue isolation, and identified based on sequence analysis of the internal transcribed spacer and morphological characterization by microscopic observations. A total of 1 175 fungal endophytes were isolated from 996 bark tissues. The fungal endophytes were classified into 35 fungal taxa belonging to 15 genera, including 1 species of Basidiomycota and 34 taxa of Ascomycota. And Alternaria alternata, Botryosphaeria dothidea, Fusarium spp., and Diaporthe conorum were the predominant species of fungal endophytes in barks of P.×euramericana cv. Robusta 94 and triploid of P. tomentosa, while A. alternata and B. dothidea were the most common predominant species in the barks of the two cultivars. The variation of predominant fungal endophytes of two poplar species in different seasons was detected.
- fungal endophytes,
- predominant species,
- diversity,
- bark

References

[1]	Petrini O.Fungal endophytes of tree leaves[M]//Andrews J H, Hirano S S. Microbial ecology of leaves New York: Springer-Verlag, 1991:179-197
[2]	孙剑秋,郭良栋,臧威, 等.药用植物内生真菌多样性及生态分布[J]. 中国科学C辑:生命科学,2008,38(5):475-484
[3]	白红霞,袁秀英. 内蒙古地区杨树内生真菌多样性调查[J]. 浙江林学院学报, 2006,23 (6): 629-635
[4]	王涛,魏淑芳,魏琴,等.油樟叶内生真菌的多样性研究[J]. 云南大学学报:自然科学版, 2007,29(3): 300-302
[5]	杜少康,陈双林,林岱,等.银杏叶部内生真菌多样性的研究[J]. 菌物学报, 2009,28(4):504-511
[6]	李淑梅,张春玲,胡建军,等.转基因抗虫杨树新品种——健杨94[J]. 林业科学, 2008,44(7):141
[7]	朱之涕.三倍体毛白杨新品种简介[J]. 北京林业大学学报, 2002,24(增刊):60-62
[8]	林彩丽,王曦茁,朴春根,等.一种用于PCR扩增的真菌DNA快速提取方法 [P]. 2009,专利号:ZL 2009 1 0235594.3
[9]	White T J, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M]//Innis M A, Gelfand D H, Sninsky J J, et al. PCR Protocols: A Guide to Methods and Applications. New York:Academic Press, 1990:315-322
[10]	魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979
[11]	By X K, Xing J, Chen M J, et al. Fungal endophytes associated with Sonneratia (Sonneratiaceae) mangrove plants on the south coast of China[J]. Forest Pathology, 2011, 41: 334-340
[12]	Petrini O, Stone J, Carroll F E. Endophytic fungi in evergreen shrubs in western Oregon: a preliminary study[J]. Can J Bot, 1982, 60:789-796
[13]	Pielou E C. Ecological Diversity[M]. New York: John Wiley and Sons Inc,1975
[14]	Stone J K. Initiation and development of latent infection by Rhabdocline parkeri on Douglas fir[J]. Can J Bot, 1987, 65: 2614-2621
[15]	Fröhlich J, Hyde K D, Petrini O. Endophytic fungi associated with palms[J]. Mycol Res, 2000, 104: 1202-1212
[16]	Larran S, Monaco C, Alippi H E. Endophytic fungi in leaves of Lycopersicon esculentum Mill[J]. World Journal of Microbiology & Biotechnology, 2001, 17: 181-184
[17]	Santamaría J, Bayman P. Fungal epiphytes and endophytes of coffee leaves (Coffea arabica)[J]. Microbial Ecology, 2005, 50: 1-8
[18]	Schulz B, Boyle C. The endophytic continuum[J]. Mycol Res 2005,109(Pt 6):661-686
[19]	Park Y H, Kim Y C, Park S U, et al. Age-dependent Distribution of Fungal Endophytes in Panax ginseng Roots Cultivated in Korea [J]. J Ginseng Res, 2012, 36(3):327-333
[20]	Park Y H, Lee S G, Ahn D J, et al. Diversity of Fungal Endophytes in Various Tissues of Panax ginseng Meyer Cultivated in Korea[J]. J Ginseng Res, 2012, 36(2): 211-217
[21]	张星耀, 骆有庆.中国森林重大生物灾害[M]. 北京: 中国林业出版社,2003
[22]	冯茜, 黄云, 巩春梅, 等. 川芎根腐病菌(Fusarium solani) 的生物学特性[J]. 四川农业大学学报, 2008,26(1):24-27
[23]	Lipscomb H,Witcher W. Canker of Tulip Poplar caused by Fusarium solani[J]. Plant Disease Reporter, 1965, 49 (6): 507-508
[24]	Nemec S. Fusarium solani association with branch and trunk cankers on citrus weakened by cold weather in Florida[J]. Mycopathologia, 1987, 97(3): 143-150
[25]	佀传杰,高桃生,李多山.健杨94的抗虫性试验[J].林业科技开发,2008,22(2):82-83
[26]	Bailey B A, Bae H, Strem M D, et al. Antibiosis, mycoparasitism, and colonization success for endophytic Trichoderma isolates with biocontrol potential in Theobroma cacao[J]. Biological Control, 2008,46: 24-35
[27]	Hanada R E, de Jorge Souza T, Pomella A W V, et al. Trichoderma martiale sp. nov., a new endophyte from sapwood of Theobroma cacao with a potential for biological control[J]. Mycological Research,2008, 112: 1335-1343
[28]	Howel C R. Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts[J]. Plant Disease, 2003, 87: 4-10
[29]	Holmes K A, Schroers H J, Thomas S E, et al. Taxonomy and biocontrol potential of a new species of Trichoderma from the Amazon basin of South America[J]. Mycological Progress, 2004, 3: 199-210

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Predominant Species Dynamic and Diversity of Fungal Endophytes in Barks of Two Populus Cultivars

1. Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China

2. Puyang Academy of Forestry, Puyang 457000, He’nan, China

3. Heze Forest Protection Station, Heze 274002, Shandong, China

4. Beijing Forestry University, Beijing 100083, China

Received Date: 2012-12-17

Abstract: In order to understand the predominant species dynamic and diversity of fungal endophytes in barks of Populus×euramericana cv. Robusta 94 and triploid of P. tomentosa,the fungal endophytes were isolated from barks of P.×euramericana cv. Robusta 94 and triploid of P. tomentosa by tissue isolation, and identified based on sequence analysis of the internal transcribed spacer and morphological characterization by microscopic observations. A total of 1 175 fungal endophytes were isolated from 996 bark tissues. The fungal endophytes were classified into 35 fungal taxa belonging to 15 genera, including 1 species of Basidiomycota and 34 taxa of Ascomycota. And Alternaria alternata, Botryosphaeria dothidea, Fusarium spp., and Diaporthe conorum were the predominant species of fungal endophytes in barks of P.×euramericana cv. Robusta 94 and triploid of P. tomentosa, while A. alternata and B. dothidea were the most common predominant species in the barks of the two cultivars. The variation of predominant fungal endophytes of two poplar species in different seasons was detected.

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Reference (29)

[1]	Petrini O.Fungal endophytes of tree leaves[M]//Andrews J H, Hirano S S. Microbial ecology of leaves New York: Springer-Verlag, 1991:179-197
[2]	孙剑秋,郭良栋,臧威, 等.药用植物内生真菌多样性及生态分布[J]. 中国科学C辑:生命科学,2008,38(5):475-484
[3]	白红霞,袁秀英. 内蒙古地区杨树内生真菌多样性调查[J]. 浙江林学院学报, 2006,23 (6): 629-635
[4]	王涛,魏淑芳,魏琴,等.油樟叶内生真菌的多样性研究[J]. 云南大学学报:自然科学版, 2007,29(3): 300-302
[5]	杜少康,陈双林,林岱,等.银杏叶部内生真菌多样性的研究[J]. 菌物学报, 2009,28(4):504-511
[6]	李淑梅,张春玲,胡建军,等.转基因抗虫杨树新品种——健杨94[J]. 林业科学, 2008,44(7):141
[7]	朱之涕.三倍体毛白杨新品种简介[J]. 北京林业大学学报, 2002,24(增刊):60-62
[8]	林彩丽,王曦茁,朴春根,等.一种用于PCR扩增的真菌DNA快速提取方法 [P]. 2009,专利号:ZL 2009 1 0235594.3
[9]	White T J, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M]//Innis M A, Gelfand D H, Sninsky J J, et al. PCR Protocols: A Guide to Methods and Applications. New York:Academic Press, 1990:315-322
[10]	魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979
[11]	By X K, Xing J, Chen M J, et al. Fungal endophytes associated with Sonneratia (Sonneratiaceae) mangrove plants on the south coast of China[J]. Forest Pathology, 2011, 41: 334-340
[12]	Petrini O, Stone J, Carroll F E. Endophytic fungi in evergreen shrubs in western Oregon: a preliminary study[J]. Can J Bot, 1982, 60:789-796
[13]	Pielou E C. Ecological Diversity[M]. New York: John Wiley and Sons Inc,1975
[14]	Stone J K. Initiation and development of latent infection by Rhabdocline parkeri on Douglas fir[J]. Can J Bot, 1987, 65: 2614-2621
[15]	Fröhlich J, Hyde K D, Petrini O. Endophytic fungi associated with palms[J]. Mycol Res, 2000, 104: 1202-1212
[16]	Larran S, Monaco C, Alippi H E. Endophytic fungi in leaves of Lycopersicon esculentum Mill[J]. World Journal of Microbiology & Biotechnology, 2001, 17: 181-184
[17]	Santamaría J, Bayman P. Fungal epiphytes and endophytes of coffee leaves (Coffea arabica)[J]. Microbial Ecology, 2005, 50: 1-8
[18]	Schulz B, Boyle C. The endophytic continuum[J]. Mycol Res 2005,109(Pt 6):661-686
[19]	Park Y H, Kim Y C, Park S U, et al. Age-dependent Distribution of Fungal Endophytes in Panax ginseng Roots Cultivated in Korea [J]. J Ginseng Res, 2012, 36(3):327-333
[20]	Park Y H, Lee S G, Ahn D J, et al. Diversity of Fungal Endophytes in Various Tissues of Panax ginseng Meyer Cultivated in Korea[J]. J Ginseng Res, 2012, 36(2): 211-217
[21]	张星耀, 骆有庆.中国森林重大生物灾害[M]. 北京: 中国林业出版社,2003
[22]	冯茜, 黄云, 巩春梅, 等. 川芎根腐病菌(Fusarium solani) 的生物学特性[J]. 四川农业大学学报, 2008,26(1):24-27
[23]	Lipscomb H,Witcher W. Canker of Tulip Poplar caused by Fusarium solani[J]. Plant Disease Reporter, 1965, 49 (6): 507-508
[24]	Nemec S. Fusarium solani association with branch and trunk cankers on citrus weakened by cold weather in Florida[J]. Mycopathologia, 1987, 97(3): 143-150
[25]	佀传杰,高桃生,李多山.健杨94的抗虫性试验[J].林业科技开发,2008,22(2):82-83
[26]	Bailey B A, Bae H, Strem M D, et al. Antibiosis, mycoparasitism, and colonization success for endophytic Trichoderma isolates with biocontrol potential in Theobroma cacao[J]. Biological Control, 2008,46: 24-35
[27]	Hanada R E, de Jorge Souza T, Pomella A W V, et al. Trichoderma martiale sp. nov., a new endophyte from sapwood of Theobroma cacao with a potential for biological control[J]. Mycological Research,2008, 112: 1335-1343
[28]	Howel C R. Mechanisms employed by Trichoderma species in the biological control of plant diseases: the history and evolution of current concepts[J]. Plant Disease, 2003, 87: 4-10
[29]	Holmes K A, Schroers H J, Thomas S E, et al. Taxonomy and biocontrol potential of a new species of Trichoderma from the Amazon basin of South America[J]. Mycological Progress, 2004, 3: 199-210

Predominant Species Dynamic and Diversity of Fungal Endophytes in Barks of Two Populus Cultivars

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通讯作者: 陈斌, bchen63@163.com

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Predominant Species Dynamic and Diversity of Fungal Endophytes in Barks of Two Populus Cultivars

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