The Impact of Drought Stress on Physiological Indicators of Four Shrub Species on the Qinghai-Tibet Plateau

PAN Xin; LI Ji-yue; WANG Jun-hui; HE Qian; SU Yan; MA Jian-wei; DU Kun

Volume 26 Issue 3

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The Impact of Drought Stress on Physiological Indicators of Four Shrub Species on the Qinghai-Tibet Plateau

2.
College of Forestry, South China Agricultural University, Guanzhou 510642, Guangdong, China
3.
Research Institute of Forestry, Chinese Academy of Forestry, Key laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China
4.
Xiaolongshan Forestry Science and Technology Research Institute, Tianshui 741022, Gansu, China

Received Date: 2012-08-10

Abstract

Four shrub species, Hippophae rhamnoides, Caragana sinica, Sophora moorcroftiana, and Caryopteris tangutica from Qinghai-Tibet Plateau were used to simulate the drought stress on potted seedlings. By comparing the changes of malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, peroxidase (POD) activity, praline content, and soluble protein content, the drought resistance performance of selected tree species were analyzed and evaluated in order to find the superior drought tolerant shrub species suitable for the afforestation on Qinghai-Tibet Plateau area. The results indicated that the MDA content rose slowly when the four selected plants were under light drought stress and reached the maximum when under heavy drought stress. Both the peak and increase of MDA content of Caryopteris tangutica were the least compared with the other three species, followed by Caragana sinica and Hippophae rhamnoides, and the peak and increase of MDA of Sophora moorcroftiana were the largest. The change rule of SOD and POD activity followed a single-peak curve, with Caryopteris tangutica and Caragana sinica's SOD activity spiked the latest and the value was also the largest. The SOD activity decreased to the minimum, which fully reflected that the two species had stronger adaptability and self-regulation under drought environment, while the Hippophae rhamnoides and Sophora moorcroftiana were the opposite. During the process of drought stress, the POD activity of Caryopteris tangutica maintained at a low level, but with the greatest increase (up to 805.23%), the increase rate was much higher than that of the other three species, and showed a superior ability of drought tolerance. But for the rest three species, the increase rate of POD activity was small and the adaptability to drought proved to be bad. The proline content of the 4 species showed an increasing trend and the increase rate of Caryopteris tangutica was the highest, 10.86 times compared with that under normal water condition. The seedling's soluble protein content increased at initial and then decreased, the soluble protein's increase of Caryopteris tangutica was the largest which was up to 213.44%. The comprehensive evaluation results showed that under water stress, the seedlings' resistance strength sequences were Caryopteris tangutica>Caragana sinica>Hippophae rhamnoides>Sophora moorcroftiana.
- drought stress,
- Qinghai-Tibet Plateau,
- physiological index

References

[1]	Apel K,Hirt H. Reactive oxygen species: metabolism,oxidative stress,and signal transduction[J]. Annual Review of Plant Biology,2004,55:373-399
[2]	张金霞,曹广民,周党卫,等. 高寒矮嵩草草甸大气-土壤-植被-动物系统碳素储量及碳素循环[J]. 生态学报,2003,23(4):627-634
[3]	邹燕,赵平. 青藏高原年代际气候变化研究进展[J].气象科技,2008,36(2): 168-173
[4]	梁坤伦,姜文清,周志宇,等.青藏高原紫穗槐主要形态特征变异分析[J].生态学报,2012,32(1):311-318
[5]	Srivalli B,Sharma G,Khanna-Chopra R. Antioxidative defense system in an upland rice cultivar subjected to increasing intensity of water stress followed by recovery[J]. Physiologia Plantarum,2003,119 ( 4): 503-512
[6]	Papadakis A K,Roubelakis-Angelakis K A. Polyamines inhibit NADPH oxidase-mediated superoxide generation and putrescine prevents programmed cell death induced by polyamine oxidase-generated hydrogen peroxide[J].Planta,2005,220(6): 826-837
[7]	王烨军,廖万有,朱振.茶树抗性生理评价研究进展[J]. 中国农学通报,2011,27(2):79-83
[8]	宋维秀,王志涛,马正华. 青藏高原区3 种经济灌木的抗旱生理研究[J]. 中南林业科技大学学报,2012,32(9):28-31
[9]	朱春云,赵越,王志涛. 国外灌木树种在青藏高原的适生性研究[J]. 河北林业科技,2005(6):14-15
[10]	罗大庆,薛会英,权红,等. 干旱胁迫下砂生槐、锦鸡儿的生理生化特性与抗旱性[J]. 干旱区资源与环境,2011,25(9):122-127
[11]	罗大庆,方江平,权红,等. 西藏半干旱区三种柏树抗旱性研究[J].资源科学,2010,32(8):1601-1607
[12]	黄颜梅,张健,罗承德. 西藏柏木抗旱生理研究[J]. 四川林业科技,1998,19(4):31-36
[13]	陈晓远,高志红,罗远培,等.不同土壤水分冬小麦根、冠关系及其对叶片水分利用效率的影响[J].中国生态农业学报,2005,13(2):134-137
[14]	蒲金涌,姚小英,贾海源,等.甘肃陇西黄土高原旱作区土壤水分变化规律及有效利用程度研究[J].土壤通报,2005,36(4):483-486
[15]	邹琪. 植物生理学试验指导.北京:中国农业出版社,2000:98-100
[16]	张志良. 植物生理学试验指导[M]. 北京:高等教育出版社,2001:123-124
[17]	张宪政, 陈凤玉. 植物生理学实验技术[M]. 沈阳: 辽宁科学技术出版社, 1994
[18]	汪家政, 范明. 蛋白质技术手册. 北京: 科学出版社, 2002
[19]	郝冉彬,苍晶,徐仲. 植物生理试验[M]. 哈尔滨: 哈尔滨工业大学出版社,2004:130-133
[20]	王韶唐. 植物生理学试验指导.西安:陕西科学技术出版社,1987:133-135
[21]	邹琦,李德全,郑国生,等. 作物抗旱生理生态研究.济南:山东科学技术出版社,1994:24-29
[22]	陆国盈,劳丽萍,韩世健,等. 5个甘蔗新品种( 系) 的抗旱性研究[J].安徽农业科学,2011,39(3): 1341-1345
[23]	潘昕,李吉跃,苏艳,等.干旱胁迫对华南地区三种苗木膜脂过氧化及保护酶活性的影响[J].广东林业科技,2012,28(1):13-18
[24]	冯祥元,于柱英,种培芳.不同种源地云杉的苗期抗旱性评价[J].甘肃农业大学学报,2012,47(1):95-102
[25]	李凤海,朱敏,吕香玲.常用玉米自交系抗旱性及抗旱性鉴定指标研究[J].种子,2011,30(1):31-34
[26]	范苏鲁,苑兆和,冯立娟,等.干旱胁迫对大丽花生理生化指标的影响[J]. 应用生态学报,2011,22(3): 651-657
[27]	李秀玲,刘开强,杨志民,等.干旱胁迫对４种观赏草枯叶率及生理指标的影响[J].草地学报,2012,20(1):76-82
[28]	柴薇薇,蒋志荣,孔东升,等.3 种灌木幼苗对干旱胁迫的生理响应[J]. 安徽农业科学, 2007, 35(5): 1273-1274, 1289
[29]	李秀玲,刘开强,杨志民,等.干旱胁迫对４种观赏草枯叶率及生理指标的影响[J].草地学报,2012,20(1):76-82
[30]	李合生. 植物生理生化试验原理和技术. 北京: 高等教育出版社, 2000: 134-200
[31]	桑子阳,马履一,陈发菊.干旱胁迫对红花玉兰幼苗生长和生理特性的影响[J].西北植物学报, 2011, 31(1): 0109- 0115
[32]	张仁和,郑友军,马国胜,等.干旱胁迫对玉米苗期叶片光合作用和保护酶的影响[J]. 生态学报,2011,31(5): 1303-1311
[33]	覃鹏,刘叶菊,刘飞虎.干旱处理对烟草叶片SOD和POD活性的影响[J].中国烟草科学,2005(2):28-30
[34]	律秀娜,任丽娜,黄真真,等.干旱胁迫对月见草体内游离脯氨酸含量的影响[J].高师理科学刊,2007(27):66-68
[35]	李波,贾秀峰,白庆武,等.干旱胁迫对首蓓脯氨酸累积的影响[J].植物研究,2003(23):189-191
[36]	马双艳,姜远茂,彭福田,等. 干旱胁迫对苹果叶片中甜菜碱和丙二醛及脯氨酸含量的影响[J].落叶果树,2003(5):1-4
[37]	XIONG Li-ming, Schumaker K S, ZHU Jian-kang.Cell signaling during cold, drought, and salt stress[J]. Plant Cell,2002, 14: 165-183
[38]	吴志华, 曾富华, 马生健. ABA对PEG胁迫下狗牙根可溶性蛋白质的影响[J]. 草业学报, 2004, 13( 5): 75-78

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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The Impact of Drought Stress on Physiological Indicators of Four Shrub Species on the Qinghai-Tibet Plateau

2. College of Forestry, South China Agricultural University, Guanzhou 510642, Guangdong, China

3. Research Institute of Forestry, Chinese Academy of Forestry, Key laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China

4. Xiaolongshan Forestry Science and Technology Research Institute, Tianshui 741022, Gansu, China

Received Date: 2012-08-10

Abstract: Four shrub species, Hippophae rhamnoides, Caragana sinica, Sophora moorcroftiana, and Caryopteris tangutica from Qinghai-Tibet Plateau were used to simulate the drought stress on potted seedlings. By comparing the changes of malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, peroxidase (POD) activity, praline content, and soluble protein content, the drought resistance performance of selected tree species were analyzed and evaluated in order to find the superior drought tolerant shrub species suitable for the afforestation on Qinghai-Tibet Plateau area. The results indicated that the MDA content rose slowly when the four selected plants were under light drought stress and reached the maximum when under heavy drought stress. Both the peak and increase of MDA content of Caryopteris tangutica were the least compared with the other three species, followed by Caragana sinica and Hippophae rhamnoides, and the peak and increase of MDA of Sophora moorcroftiana were the largest. The change rule of SOD and POD activity followed a single-peak curve, with Caryopteris tangutica and Caragana sinica's SOD activity spiked the latest and the value was also the largest. The SOD activity decreased to the minimum, which fully reflected that the two species had stronger adaptability and self-regulation under drought environment, while the Hippophae rhamnoides and Sophora moorcroftiana were the opposite. During the process of drought stress, the POD activity of Caryopteris tangutica maintained at a low level, but with the greatest increase (up to 805.23%), the increase rate was much higher than that of the other three species, and showed a superior ability of drought tolerance. But for the rest three species, the increase rate of POD activity was small and the adaptability to drought proved to be bad. The proline content of the 4 species showed an increasing trend and the increase rate of Caryopteris tangutica was the highest, 10.86 times compared with that under normal water condition. The seedling's soluble protein content increased at initial and then decreased, the soluble protein's increase of Caryopteris tangutica was the largest which was up to 213.44%. The comprehensive evaluation results showed that under water stress, the seedlings' resistance strength sequences were Caryopteris tangutica>Caragana sinica>Hippophae rhamnoides>Sophora moorcroftiana.

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Reference (38)

[1]	Apel K,Hirt H. Reactive oxygen species: metabolism,oxidative stress,and signal transduction[J]. Annual Review of Plant Biology,2004,55:373-399
[2]	张金霞,曹广民,周党卫,等. 高寒矮嵩草草甸大气-土壤-植被-动物系统碳素储量及碳素循环[J]. 生态学报,2003,23(4):627-634
[3]	邹燕,赵平. 青藏高原年代际气候变化研究进展[J].气象科技,2008,36(2): 168-173
[4]	梁坤伦,姜文清,周志宇,等.青藏高原紫穗槐主要形态特征变异分析[J].生态学报,2012,32(1):311-318
[5]	Srivalli B,Sharma G,Khanna-Chopra R. Antioxidative defense system in an upland rice cultivar subjected to increasing intensity of water stress followed by recovery[J]. Physiologia Plantarum,2003,119 ( 4): 503-512
[6]	Papadakis A K,Roubelakis-Angelakis K A. Polyamines inhibit NADPH oxidase-mediated superoxide generation and putrescine prevents programmed cell death induced by polyamine oxidase-generated hydrogen peroxide[J].Planta,2005,220(6): 826-837
[7]	王烨军,廖万有,朱振.茶树抗性生理评价研究进展[J]. 中国农学通报,2011,27(2):79-83
[8]	宋维秀,王志涛,马正华. 青藏高原区3 种经济灌木的抗旱生理研究[J]. 中南林业科技大学学报,2012,32(9):28-31
[9]	朱春云,赵越,王志涛. 国外灌木树种在青藏高原的适生性研究[J]. 河北林业科技,2005(6):14-15
[10]	罗大庆,薛会英,权红,等. 干旱胁迫下砂生槐、锦鸡儿的生理生化特性与抗旱性[J]. 干旱区资源与环境,2011,25(9):122-127
[11]	罗大庆,方江平,权红,等. 西藏半干旱区三种柏树抗旱性研究[J].资源科学,2010,32(8):1601-1607
[12]	黄颜梅,张健,罗承德. 西藏柏木抗旱生理研究[J]. 四川林业科技,1998,19(4):31-36
[13]	陈晓远,高志红,罗远培,等.不同土壤水分冬小麦根、冠关系及其对叶片水分利用效率的影响[J].中国生态农业学报,2005,13(2):134-137
[14]	蒲金涌,姚小英,贾海源,等.甘肃陇西黄土高原旱作区土壤水分变化规律及有效利用程度研究[J].土壤通报,2005,36(4):483-486
[15]	邹琪. 植物生理学试验指导.北京:中国农业出版社,2000:98-100
[16]	张志良. 植物生理学试验指导[M]. 北京:高等教育出版社,2001:123-124
[17]	张宪政, 陈凤玉. 植物生理学实验技术[M]. 沈阳: 辽宁科学技术出版社, 1994
[18]	汪家政, 范明. 蛋白质技术手册. 北京: 科学出版社, 2002
[19]	郝冉彬,苍晶,徐仲. 植物生理试验[M]. 哈尔滨: 哈尔滨工业大学出版社,2004:130-133
[20]	王韶唐. 植物生理学试验指导.西安:陕西科学技术出版社,1987:133-135
[21]	邹琦,李德全,郑国生,等. 作物抗旱生理生态研究.济南:山东科学技术出版社,1994:24-29
[22]	陆国盈,劳丽萍,韩世健,等. 5个甘蔗新品种( 系) 的抗旱性研究[J].安徽农业科学,2011,39(3): 1341-1345
[23]	潘昕,李吉跃,苏艳,等.干旱胁迫对华南地区三种苗木膜脂过氧化及保护酶活性的影响[J].广东林业科技,2012,28(1):13-18
[24]	冯祥元,于柱英,种培芳.不同种源地云杉的苗期抗旱性评价[J].甘肃农业大学学报,2012,47(1):95-102
[25]	李凤海,朱敏,吕香玲.常用玉米自交系抗旱性及抗旱性鉴定指标研究[J].种子,2011,30(1):31-34
[26]	范苏鲁,苑兆和,冯立娟,等.干旱胁迫对大丽花生理生化指标的影响[J]. 应用生态学报,2011,22(3): 651-657
[27]	李秀玲,刘开强,杨志民,等.干旱胁迫对４种观赏草枯叶率及生理指标的影响[J].草地学报,2012,20(1):76-82
[28]	柴薇薇,蒋志荣,孔东升,等.3 种灌木幼苗对干旱胁迫的生理响应[J]. 安徽农业科学, 2007, 35(5): 1273-1274, 1289
[29]	李秀玲,刘开强,杨志民,等.干旱胁迫对４种观赏草枯叶率及生理指标的影响[J].草地学报,2012,20(1):76-82
[30]	李合生. 植物生理生化试验原理和技术. 北京: 高等教育出版社, 2000: 134-200
[31]	桑子阳,马履一,陈发菊.干旱胁迫对红花玉兰幼苗生长和生理特性的影响[J].西北植物学报, 2011, 31(1): 0109- 0115
[32]	张仁和,郑友军,马国胜,等.干旱胁迫对玉米苗期叶片光合作用和保护酶的影响[J]. 生态学报,2011,31(5): 1303-1311
[33]	覃鹏,刘叶菊,刘飞虎.干旱处理对烟草叶片SOD和POD活性的影响[J].中国烟草科学,2005(2):28-30
[34]	律秀娜,任丽娜,黄真真,等.干旱胁迫对月见草体内游离脯氨酸含量的影响[J].高师理科学刊,2007(27):66-68
[35]	李波,贾秀峰,白庆武,等.干旱胁迫对首蓓脯氨酸累积的影响[J].植物研究,2003(23):189-191
[36]	马双艳,姜远茂,彭福田,等. 干旱胁迫对苹果叶片中甜菜碱和丙二醛及脯氨酸含量的影响[J].落叶果树,2003(5):1-4
[37]	XIONG Li-ming, Schumaker K S, ZHU Jian-kang.Cell signaling during cold, drought, and salt stress[J]. Plant Cell,2002, 14: 165-183
[38]	吴志华, 曾富华, 马生健. ABA对PEG胁迫下狗牙根可溶性蛋白质的影响[J]. 草业学报, 2004, 13( 5): 75-78

The Impact of Drought Stress on Physiological Indicators of Four Shrub Species on the Qinghai-Tibet Plateau

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通讯作者: 陈斌, bchen63@163.com

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The Impact of Drought Stress on Physiological Indicators of Four Shrub Species on the Qinghai-Tibet Plateau

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