Sequence Analysis and Chilling Resistance about a New CBF-like Transcription Factor Fragment from Jacaranda acutifolia

ZHOU Jing; ZHANG Wei; LI Da-ming; ZHANG Xi-jiu; CHEN Qi-bing

Volume 26 Issue 4

Article Contents

Turn off MathJax

Article Navigation > Forest Research > 2013 > 26(4): 414-419

Citation:

Sequence Analysis and Chilling Resistance about a New CBF-like Transcription Factor Fragment from Jacaranda acutifolia

1.
College of Landscape Architecture, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
2.
College of Life Science, Sichuan Normal University, Chengdu 610066, Sichuan, China
3.
Sichuan Academy of Forestry, Chengdu 610081, Sichuan, China

Received Date: 2012-11-28

Abstract

CBFs (CRT/DRE-binding factor) are key transcription factors regulating the chilling response in plants. A 457 bp CBF-like fragment was cloned from Jacaranda acutifolia. Sequence analysis showed that the fragment (named as JaCBF1) encoded a protein of 142 amino acids, and was highly homologous to many known CBF1-like transcription factors with the AP2 core domain that belongs to CBF gene family. The recombinant plasmid containing JaCBF1 sequence was introduced into Escherichia coli. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that JaCBF1 was translated into a 16 KDa protein. Expression pattern analysis indicated that JaCBF1 could be induced with different patterns and degrees by low temperature in roots, stems, and leaves of J. acutifolia. Southern blot revealed that the J. acutifolia genome contained at least two copies of JaCBF1. Ectopic transcription in Arabidopsis thaliana revealed that JaCBF1 functioned in the nuclei, and its expression could significantly improve the freezing resistance of transgenic A. thaliana. The results of study suggest that the novel JaCBF1 is a typical CBF-like transcription factor, and plays an important role responding to cold stress in J.acutifolia.
- Jacaranda acutifolia,
- CBF,
- transcription factor,
- induced expression,
- chilling resistance

References

[1]	Enevoldsen E. CBF in head injury[J]. Acta Neurochir Suppl (Wien), 1986, 36:133-136
[2]	Friedman A D. Leukemogenesis by CBF oncoproteins[J]. Leukemia, 1999, 13 (12):1932-1942
[3]	Gilbert J. Estimation of CBF by cerebral venous oxygen difference[J]. J Neurosurg, 1989, 71 (5 Pt 1):790-791
[4]	Ito Y, Katsura K, Maruyama K, et al. Functional analysis of rice DREB1/CBF-type transcription factors involved in cold-responsive gene expression in transgenic rice[J]. Plant Cell Physiol, 2006, 47 (1):141-153
[5]	Tamura K, Yamada T. A perennial ryegrass CBF gene cluster is located in a region predicted by conserved synteny between Poaceae species[J]. Theor Appl Genet, 2007, 114 (2):273-283
[6]	Xiong Y, Fei S Z. Functional and phylogenetic analysis of a DREB/CBF-like gene in perennial ryegrass (Lolium perenne L.) [J]. Planta, 2006, 224 (4):878-888
[7]	Mboup M, Fischer I, Lainer H, et al. Trans-species polymorphism and allele-specific expression in the CBF gene family of wild tomatoes[J]. Mol Biol Evol, 2012, 29 (12):3641-3652
[8]	Zhang X, Fowler S G, Cheng H, et al. Freezing-sensitive tomato has a functional CBF cold response pathway, but a CBF regulon that differs from that of freezing-tolerant Arabidopsis[J]. Plant J, 2004, 39 (6):905-919
[9]	金万梅, 董静, 尹淑萍, 等. 冷诱导转录因子CBF1转化草莓及其抗寒性鉴定[J]. 西北植物学报, 2007(2):223-227
[10]	张勇. 草莓冷诱导转录因CBF的克隆与结构分析及其抗寒特性研究. 四川雅安:四川农业大学, 2009
[11]	刘荣梅, 李凤兰, 胡国富, 等. 转CBF3基因烟草的抗寒性研究[J]. 东北农业大学学报, 2011(1):119-123
[12]	Takumi S, Shimamura C, Kobayashi F. Increased freezing tolerance through up-regulation of downstream genes via the wheat CBF gene in transgenic tobacco[J]. Plant Physiol Biochem, 2008, 46 (2):205-211
[13]	Sinha S, Maity S N, Lu J, et al. Recombinant rat CBF-C, the third subunit of CBF/NFY, allows formation of a protein-DNA complex with CBF-A and CBF-B and with yeast HAP2 and HAP3[J]. Proc Natl Acad Sci U S A, 1995, 92 (5):1624-1628
[14]	Gilmour S J, Zarka D G, Stockinger E J, et al. Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression[J]. Plant J, 1998, 16 (4):433-442
[15]	Medina J, Bargues M, Terol J, et al. The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression Is regulated by low temperature but not by abscisic acid or dehydration[J]. Plant Physiol, 1999, 119 (2):463-470
[16]	Jaglo K R, Kleff S, Amundsen K L, et al. Components of the Arabidopsis C-repeat/dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species[J]. Plant Physiol, 2001, 127 (3):910-917
[17]	Jayasinghe D, Gill A B, Levene M I. CBF reactivity in hypotensive and normotensive preterm infants[J]. Pediatr Res, 2003, 54 (6):848-853
[18]	吕胜男, 生吉萍, 赵丹莹, 等. CBF基因调控植物抗冷径途的研究进展[J]. 西北植物学报, 2011(6):1275-1281
[19]	刘湘. 一种蓝花楹属植物的植化和抗菌活性的研究[J]. 国外医药(植物药分册), 1992 (3):127
[20]	Hilario E, Mackay J. Protocols for Nucleic Acid Analysis by Nonradioactive Probes[M]. Humana Press, 2007, x:321
[21]	Kuo J. Electron Microscopy: Methods and Protocols[M]. Humana Press, 2007, xv:608
[22]	Barnes S H. Ultrastructural imaging of freeze-fractured plant cells in the scanning electron microscope[J]. Microsc Res Tech, 1992, 22 (2):160-169
[23]	Singh A, Sarkar A, Singh S, et al. Investigation of supplemental ultraviolet-B-induced changes in antioxidative defense system and leaf proteome in radish (Raphanus sativus L. cv Truthful): an insight to plant response under high oxidative stress[J]. Protoplasma, 2010, 245 (1-4):75-83
[24]	Morrow D, Cullen J P, Cahill P A, et al. Cyclic strain regulates the Notch/CBF-1 signaling pathway in endothelial cells: role in angiogenic activity[J]. Arterioscler Thromb Vasc Biol, 2007, 27 (6):1289-1296
[25]	Dutta A, Sen J, Deswal R. Downregulation of terpenoid indole alkaloid biosynthetic pathway by low temperature and cloning of a AP2 type C-repeat binding factor (CBF) from Catharanthus roseus (L). G. Don[J]. Plant Cell Rep, 2007, 26 (10):1869-1878
[26]	Wang Y, Hua J. A moderate decrease in temperature induces COR15a expression through the CBF signaling cascade and enhances freezing tolerance[J]. Plant J, 2009, 60 (2):340-349

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(3049) PDF downloads(1229) Cited by()

Proportional views

Sequence Analysis and Chilling Resistance about a New CBF-like Transcription Factor Fragment from Jacaranda acutifolia

1. College of Landscape Architecture, Sichuan Agricultural University, Chengdu 611130, Sichuan, China

2. College of Life Science, Sichuan Normal University, Chengdu 610066, Sichuan, China

3. Sichuan Academy of Forestry, Chengdu 610081, Sichuan, China

Received Date: 2012-11-28

Abstract: CBFs (CRT/DRE-binding factor) are key transcription factors regulating the chilling response in plants. A 457 bp CBF-like fragment was cloned from Jacaranda acutifolia. Sequence analysis showed that the fragment (named as JaCBF1) encoded a protein of 142 amino acids, and was highly homologous to many known CBF1-like transcription factors with the AP2 core domain that belongs to CBF gene family. The recombinant plasmid containing JaCBF1 sequence was introduced into Escherichia coli. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that JaCBF1 was translated into a 16 KDa protein. Expression pattern analysis indicated that JaCBF1 could be induced with different patterns and degrees by low temperature in roots, stems, and leaves of J. acutifolia. Southern blot revealed that the J. acutifolia genome contained at least two copies of JaCBF1. Ectopic transcription in Arabidopsis thaliana revealed that JaCBF1 functioned in the nuclei, and its expression could significantly improve the freezing resistance of transgenic A. thaliana. The results of study suggest that the novel JaCBF1 is a typical CBF-like transcription factor, and plays an important role responding to cold stress in J.acutifolia.

HTML

Reference (26)

[1]	Enevoldsen E. CBF in head injury[J]. Acta Neurochir Suppl (Wien), 1986, 36:133-136
[2]	Friedman A D. Leukemogenesis by CBF oncoproteins[J]. Leukemia, 1999, 13 (12):1932-1942
[3]	Gilbert J. Estimation of CBF by cerebral venous oxygen difference[J]. J Neurosurg, 1989, 71 (5 Pt 1):790-791
[4]	Ito Y, Katsura K, Maruyama K, et al. Functional analysis of rice DREB1/CBF-type transcription factors involved in cold-responsive gene expression in transgenic rice[J]. Plant Cell Physiol, 2006, 47 (1):141-153
[5]	Tamura K, Yamada T. A perennial ryegrass CBF gene cluster is located in a region predicted by conserved synteny between Poaceae species[J]. Theor Appl Genet, 2007, 114 (2):273-283
[6]	Xiong Y, Fei S Z. Functional and phylogenetic analysis of a DREB/CBF-like gene in perennial ryegrass (Lolium perenne L.) [J]. Planta, 2006, 224 (4):878-888
[7]	Mboup M, Fischer I, Lainer H, et al. Trans-species polymorphism and allele-specific expression in the CBF gene family of wild tomatoes[J]. Mol Biol Evol, 2012, 29 (12):3641-3652
[8]	Zhang X, Fowler S G, Cheng H, et al. Freezing-sensitive tomato has a functional CBF cold response pathway, but a CBF regulon that differs from that of freezing-tolerant Arabidopsis[J]. Plant J, 2004, 39 (6):905-919
[9]	金万梅, 董静, 尹淑萍, 等. 冷诱导转录因子CBF1转化草莓及其抗寒性鉴定[J]. 西北植物学报, 2007(2):223-227
[10]	张勇. 草莓冷诱导转录因CBF的克隆与结构分析及其抗寒特性研究. 四川雅安:四川农业大学, 2009
[11]	刘荣梅, 李凤兰, 胡国富, 等. 转CBF3基因烟草的抗寒性研究[J]. 东北农业大学学报, 2011(1):119-123
[12]	Takumi S, Shimamura C, Kobayashi F. Increased freezing tolerance through up-regulation of downstream genes via the wheat CBF gene in transgenic tobacco[J]. Plant Physiol Biochem, 2008, 46 (2):205-211
[13]	Sinha S, Maity S N, Lu J, et al. Recombinant rat CBF-C, the third subunit of CBF/NFY, allows formation of a protein-DNA complex with CBF-A and CBF-B and with yeast HAP2 and HAP3[J]. Proc Natl Acad Sci U S A, 1995, 92 (5):1624-1628
[14]	Gilmour S J, Zarka D G, Stockinger E J, et al. Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression[J]. Plant J, 1998, 16 (4):433-442
[15]	Medina J, Bargues M, Terol J, et al. The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression Is regulated by low temperature but not by abscisic acid or dehydration[J]. Plant Physiol, 1999, 119 (2):463-470
[16]	Jaglo K R, Kleff S, Amundsen K L, et al. Components of the Arabidopsis C-repeat/dehydration-responsive element binding factor cold-response pathway are conserved in Brassica napus and other plant species[J]. Plant Physiol, 2001, 127 (3):910-917
[17]	Jayasinghe D, Gill A B, Levene M I. CBF reactivity in hypotensive and normotensive preterm infants[J]. Pediatr Res, 2003, 54 (6):848-853
[18]	吕胜男, 生吉萍, 赵丹莹, 等. CBF基因调控植物抗冷径途的研究进展[J]. 西北植物学报, 2011(6):1275-1281
[19]	刘湘. 一种蓝花楹属植物的植化和抗菌活性的研究[J]. 国外医药(植物药分册), 1992 (3):127
[20]	Hilario E, Mackay J. Protocols for Nucleic Acid Analysis by Nonradioactive Probes[M]. Humana Press, 2007, x:321
[21]	Kuo J. Electron Microscopy: Methods and Protocols[M]. Humana Press, 2007, xv:608
[22]	Barnes S H. Ultrastructural imaging of freeze-fractured plant cells in the scanning electron microscope[J]. Microsc Res Tech, 1992, 22 (2):160-169
[23]	Singh A, Sarkar A, Singh S, et al. Investigation of supplemental ultraviolet-B-induced changes in antioxidative defense system and leaf proteome in radish (Raphanus sativus L. cv Truthful): an insight to plant response under high oxidative stress[J]. Protoplasma, 2010, 245 (1-4):75-83
[24]	Morrow D, Cullen J P, Cahill P A, et al. Cyclic strain regulates the Notch/CBF-1 signaling pathway in endothelial cells: role in angiogenic activity[J]. Arterioscler Thromb Vasc Biol, 2007, 27 (6):1289-1296
[25]	Dutta A, Sen J, Deswal R. Downregulation of terpenoid indole alkaloid biosynthetic pathway by low temperature and cloning of a AP2 type C-repeat binding factor (CBF) from Catharanthus roseus (L). G. Don[J]. Plant Cell Rep, 2007, 26 (10):1869-1878
[26]	Wang Y, Hua J. A moderate decrease in temperature induces COR15a expression through the CBF signaling cascade and enhances freezing tolerance[J]. Plant J, 2009, 60 (2):340-349

Sequence Analysis and Chilling Resistance about a New CBF-like Transcription Factor Fragment from Jacaranda acutifolia

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

Sequence Analysis and Chilling Resistance about a New CBF-like Transcription Factor Fragment from Jacaranda acutifolia

HTML

Catalog

Export File

Citation

Format

Content