Factors Affecting Induction of Embryogenic Callus of Larix principis-rupprechtii
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1.
National Engineering Laboratory for Tree Breeding/Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education/The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of State Forestry Administration/College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
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Received Date:
2013-02-28
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Abstract
Taking mature zygotic embryos of Larix principis-rupprechtii as explants to induce embryogenic callus. Several factors influencing the induction were assayed by using completely random design, orthogonal design together with tissue culture technology in order to explore embryogenic callus induction in the early stage in larch and optimize its somatic embryogenesis system. The results show that the best way of disinfection during the induction of embryogenic callus is peeling, removing endosperm after disinfection of 30 minutes with 2% NaClO. The contamination rate and disinfection time are negatively correlated: the longer the sterilization time, the lower the contamination rate. The optimal agar concentration of embryogenic callus induction is 4 g·L-1, and the higher or lower of the agar concentration will not only reduce the induction rate, increase the level of callus browning, but also affect the development of explants. The results of orthogonal design for plant growth exogenous hormone show that the 2,4-D is the key factor and has a significant influence, followed is KT and 6-BA. The best combination of plant growth exogenous hormone is 2,4-D 1.8 mg·L-1 + 6-BA 0.3 mg·L-1 + KT 0.3 mg·L-1.
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