• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

Citation:

Analysis on Genetic Diversity of Broussonetia papyrifera Distributed in River Basin of Yunnan Province

  • Received Date: 2013-04-22
  • Ninty Broussonetia papyrifera samples were collected from four river basins in Yunnan province and used as materials, and AFLP markers were employed to analyze their genetic diversity. The results showed that total 786 unambiguous bands were revealed from selected 7 primer combinations, of which 632 bands were polymorphic, and the ratio of polymorphic bands was 80.4 %. An average of 90.3 polymorphic bands was detected from each primer combination on average. Among four B. papyrifera populations, the population of Jinsha-river Basin held the highest level of genetic diversity, the Nei's gene diversity being 0.145 5, while the population of Yuanjiang-river Basin held the lowest level of genetic diversity, the Nei's gene diversity being 0.112 9. The genetic differentiation coefficient was 0.038 6, which indicated that the genetic variation of individuals within population being the main sources of genetic diversity in B. papyrifera. The four populations of B. papyrifera could be divided into two groups with the genetic distance threshold 0.003. The group I consisted of only the population of Jinsha-river Basin,and group II included the other three populations of B. papyrifera. This research results could provide some references for the protection and utilization of B. papyrifera resources.
  • 加载中
  • [1]

    Zerega N J C, Clement W L, Datwyler S L, et al. Biogeography and divergence times in the mulberry family (Moraceae) [J]. Molecular Phylogenetics and Evolution, 2005, 37: 402-416
    [2] 浦同省,李 昆,廖声熙.我国长纤维树种构树的研究现状与发展前景[J].现代林业科技,2008(24):19-21

    [3] 刘志远,范卫红,沈世华.构树SRAP分子标记[J].林业科学,2009,45(12):54-58

    [4] 廖声熙,何承忠,李 昆,等.金沙江干热河谷地区构树的AFLP分析[J].西北植物学报,2007,27(12):2393-2398

    [5]

    Seelenfreund D, Piña R, Ho K Y, et al. Molecular analysis of Broussonetia papyrifera (L.) Vent. (Magnoliophyta: Urticales) from the Pacific, based on ribosomal sequences of nuclear DNA[J]. New Zealand Journal of Botany, 2011, 49(3): 413-420
    [6]

    Murray M G, Thompson W F. Rapid isolation of high-molecular-weight plant DNA [J]. Nucleic Acids Research, 1980, 8: 4321-4325
    [7]

    Vos P, Hogers R, Bleeker M, et al. AFLP: A new concept for DNA [J]. Nucleic Acids Research, 1995, 23: 4407-4414
    [8]

    Tixier M H, Sourdille P, Roder M, et al. Detection of wheat microsatellites using a non radioactive silver-nitrate staining method [J]. Journal of Genetics and Breeding, 1997, 51:175-177
    [9]

    Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals[J]. Genetics, 1978, 89: 583-590
    [10]

    Li J, Chen K Y, Li Bosheng. The variation of genetic diversitity of Querus aquifoliodes in different elevations[J]. Acta Botanica Sinica,1998, 40(8): 761-767
    [11]

    McKeand S E, Eriksson G, Roberds J H. Genotype by environment interaction for index traits that combine growth and wood density in loblolly pine[J]. Theoretical and Applied Genetics, 1997,94(8):1015-1022
    [12]

    Sykes R, Li B L, Isik F, et al. Genetic variation and genotype by environment interactions of juvenile wood chemical properties in Pinus taeda L. [J]. Annals of forest science, 2006, 63(8):897-904
    [13]

    Pot D, Chantre G, Rozenberg P, et al. Genetic control of pulp and timber properties in maritime pine (Pinus pinaster Ait.) [J]. Annals of forest science, 2002, 59(5-6):563-575
    [14]

    Poke F S, Potts B M, Vaillancourt R E, et al. Genetic parameters for lignin, extractives and decay in Eucalyptus globules[J] . Annals of forest science, 2006, 63(8):813-821
    [15]

    Slatkin M. Gene flow in natural populations[J]. Annu Reu Ecol Syst, 1985, 16: 393-430
    [16]

    Lenormand T, Guillemaud T, Bourguet D, et al. Evaluating gene flow using selected markers: a case study[J]. Genetics, 1998, 149: 1383-1392
    [17]

    Whitlock M C, David E M. Indirect measures of gene flow and migration: FST≠1/(4Nm+1)[J]. Heredity, 1999, 82: 117-125
    [18]

    Wright S. The genetical structure of populations[J]. Annals of Eugenics, 1951, 15: 323-334
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article views(2940) PDF downloads(1141) Cited by()

Proportional views

Analysis on Genetic Diversity of Broussonetia papyrifera Distributed in River Basin of Yunnan Province

  • 1. Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, Yunnan, China

Abstract: Ninty Broussonetia papyrifera samples were collected from four river basins in Yunnan province and used as materials, and AFLP markers were employed to analyze their genetic diversity. The results showed that total 786 unambiguous bands were revealed from selected 7 primer combinations, of which 632 bands were polymorphic, and the ratio of polymorphic bands was 80.4 %. An average of 90.3 polymorphic bands was detected from each primer combination on average. Among four B. papyrifera populations, the population of Jinsha-river Basin held the highest level of genetic diversity, the Nei's gene diversity being 0.145 5, while the population of Yuanjiang-river Basin held the lowest level of genetic diversity, the Nei's gene diversity being 0.112 9. The genetic differentiation coefficient was 0.038 6, which indicated that the genetic variation of individuals within population being the main sources of genetic diversity in B. papyrifera. The four populations of B. papyrifera could be divided into two groups with the genetic distance threshold 0.003. The group I consisted of only the population of Jinsha-river Basin,and group II included the other three populations of B. papyrifera. This research results could provide some references for the protection and utilization of B. papyrifera resources.

Reference (18)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return