Study on the Mass Propagation of Lymantria dispar Nuclear Polyhedrosis Virus (LdNPV) and Its Application

LIANG Hong-zhu; CHEN Qian; TIAN Hui-peng; LIANG Xiao-mei

The influence of instar of the host larvae and the concentration of Lymantria dispar nuclear polyhedrosis virus (LdNPV) on larvae survival rate, larvae mass, dead body mass and the mean number of LdNPV per larval of dead body were studied. And also, the effect of feeding time on the toxicity of LdNPV was studied. The results indicated that the influence of host larvae and the concentration of LdNPV on the survival rate and larvae mass were significantly different among individuals. The younger the instar of the host larvae, the higher the concentration of LdNPV, the earlier the dead time, the higher the mortality rate, the slower the body mass' increase and the lower the toxicity. The relationship between the feeding time and the concentration of LdNPV followed a parabolic curve, i.e. both the shorter or longer time resulted in lower LdNPV concentration. It was proved that the 2nd or the 5th larvae after feeding with LdNPV (78%) were not suitable for LdNPV production, so the authors believe that the 4th instar larval fed with virus at the 1.0×106 PIB·mL-1 concentration for 10days is the most suitable condition for the mass production of LdNPV in laboratory.

1. Beijing Experimental Forest Farm, Beijing 100093, China

Received Date: 2013-03-15

Abstract: The influence of instar of the host larvae and the concentration of Lymantria dispar nuclear polyhedrosis virus (LdNPV) on larvae survival rate, larvae mass, dead body mass and the mean number of LdNPV per larval of dead body were studied. And also, the effect of feeding time on the toxicity of LdNPV was studied. The results indicated that the influence of host larvae and the concentration of LdNPV on the survival rate and larvae mass were significantly different among individuals. The younger the instar of the host larvae, the higher the concentration of LdNPV, the earlier the dead time, the higher the mortality rate, the slower the body mass' increase and the lower the toxicity. The relationship between the feeding time and the concentration of LdNPV followed a parabolic curve, i.e. both the shorter or longer time resulted in lower LdNPV concentration. It was proved that the 2nd or the 5th larvae after feeding with LdNPV (78%) were not suitable for LdNPV production, so the authors believe that the 4th instar larval fed with virus at the 1.0×106 PIB·mL-1 concentration for 10days is the most suitable condition for the mass production of LdNPV in laboratory.

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Study on the Mass Propagation of Lymantria dispar Nuclear Polyhedrosis Virus (LdNPV) and Its Application

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通讯作者: 陈斌, bchen63@163.com

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