Cloning and Expression Analysis of Circadian Clock Gene CCA1 in Populus

LIU Wu-shuang; DU Ming-hui; TAO Wei-kang; YANG Zhen; ZHUGE Qiang

Volume 26 Issue 5

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Cloning and Expression Analysis of Circadian Clock Gene CCA1 in Populus

1.
Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu, China

Received Date: 2013-05-29

Abstract

CCA1 gene plays an important role in circadian clock sensitivity in Populus. In this study, PtCCA1 was isolated from Populus tomentosa by RT-PCR. It has an open reading frame (ORF) of 1902 bp in length and encodes a protein of 663 amino acid residues, with protein molecular weight of 68.90 kDa and isoelectric point of 6.33. The amino acid sequence contained one Myb-like DNA binding domains and two putative transmembrane spanning regions. The results of fluorescence quantitative RT-PCR showed that the PtCCA1 gene was expressed mainly in leaf tissues. Along with the change of illumination time, the expression amount of PtCCA1 gene decreased during the day but gradually increased during the night. This study provides some references for further study of PtCCA1 function in regulating photoperiod sensitivity and the mechanism of circadian synchronization in poplars.
- Populus,
- PtCCA1,
- circadian clock,
- gene expression

References

[1]	Michael T P, Salomé P A, Yu H J, et al. Enhanced fitness conferred by naturally occurring variation in the circadian clock [J]. Science , 2003,302(5647): 1049-1053
[2]	Dodd A N, Salathia N, Hall A, et al. Plant circadian clocks increase photosynthesis, growth, survival, and competitive advantage [J]. Science,2005, 309(5734): 630-633
[3]	Ni Z, Kim E D, Ha M, et al. Altered circadian rhythms regulate growth vigour in hybrids and allopolyploids [J]. Nature,2009, 457(7227): 327-331
[4]	Rund-Kangisser S, Yakir E, Green R. Proteasomal regulation of CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) stability is part of the complex control of CCA1[J]. Plant Signal Behav,2013, 8(3). pii: e23206
[5]	Gutierrez R A, Stokes T L, Thum K, et al. Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1[J]. Proc Natl Acad Sci USA,2008,105(12): 4939-4944
[6]	Niwa Y, Ito S, Nakamichi N, et al. Genetic linkages of the circadian clock-associated genes, TOC1, CCA1 and LHY, in the photoperiodic control of flowering time in Arabidopsis thaliana[J]. Plant Cell Physiol,2007,48(7): 925-937
[7]	Kawamura M, Ito S, Nakamichi N,et al. The function of the clock-associated transcriptional regulator CCA1 (CIRCADIAN CLOCK-ASSOCIATED 1) in Arabidopsis thaliana[J]. Biosci Biotechnol Biochem,2008,72(5): 1307-1316
[8]	Alabadi D, Yanovsky M, Mas P, et al. Critical role for CCA1 and LHY in maintaining circadian rhythmicity in Arabidopsis[J]. Curr Biol,2002,12(9):757-761
[9]	Matsushika A, Makino S, Kojima M, et al. The APRR1/TOC1 quintet implicated in circadian rhythms of Arabidopsis thaliana: II. Characterization with CCA1-overexpressing plants[J]. Plant Cell Physiol,2002, 43(1): 118-122
[10]	邢国芳, 宋萌, 姚涵, 等. 大麦(Hordeum vulgare)昼夜节律钟基因CCA1的克隆及表达分析[J]. 山西农业大学学报,2012, 32(3): 207-212
[11]	邢国芳, 杜伟建, 张雁明, 等. 玉米昼夜节律钟基因CCA1的克隆及表达分析[J]. 山西农业大学学报,2011,31(4): 332-337
[12]	Tamura K, Peterson D, Peterson N, et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods [J]. Molecular Biology and Evolution,2011, 28(10): 2731-2739
[13]	Tuskan G. A, Difazio S, Jansson S,et al. The genome of black cottonwood, Populus trichocarpa (Torr. & Gray) [J]. Science,2006,313(5793): 1596-1604
[14]	Jansson S,Douglas C J. Populus: a model system for plant biology[J]. Annu Rev Plant Biol,2007, 58: 435-458
[15]	Daniel X, Sugano S, Tobin E M, et al. CK2 phosphorylation of CCA1 is necessary for its circadian oscillator function in Arabidopsis[J]. Proc Natl Acad Sci USA, 2004, 101(9): 3292-3297
[16]	Lu S X, Webb C J, Knowles S M, et al. CCA1 and ELF3 Interact in the control of hypocotyl length and flowering time in Arabidopsis[J]. Plant Physiol, 2012, 158(2): 1079-1088
[17]	Mizoguchi T, Wheatley K, Hanzawa Y, et al. LHY and CCA1 are partially redundant genes required to maintain circadian rhythms in Arabidopsis[J]. Dev Cell, 2002, 2(5): 629-641
[18]	Ito S, Nakamichi N, Nakamura Y, et al. Genetic linkages between circadian clock-associated components and phytochrome-dependent red light signal transduction in Arabidopsis thaliana[J]. Plant Cell Physiol, 2007, 48(7):971-983
[19]	Alabadi D, Oyama T, Yanovsky M, et al. Reciprocal regulation between TOCl and LHY/CCAl within the Arabidopsis circadian clock[J]. Science, 2001, 293(553I): 880-883
[20]	Kikis E A, Khanna R, Quail P H, et al. ELF4 is a phytochrome-regulated component of a negative-feedback loop involving the cntral oscillator components CCA1 and LHY[J]. Plant J, 2005, 44(2): 300-313
[21]	Fujimori T, Sato E, Yamashino Y, et al. PRR5 (PSEUDO-RESPONSE REGULATOR 5) plays antagonistic roles to CCA1 (CIRCADIAN CLOCK-ASSOCIATED 1) in Arabidopsis thaliana[J]. Biosci Biotechnol Biochem, 2005, 69(2): 426-430
[22]	Wang X, Wu L, Zhang S, et al. Robust expression and association of ZmCCA1 with circadian rhythms in maize [J]. Plant Cell Rep, 2011, 30(7): 1261-1272
[23]	Ovadia A, Tabibian-Keissar H, Cohen Y, et al. The 5'UTR of CCA1 includes an autoregulatory cis element that segregates between light and circadian regulation of CCA1 and LHY[J]. Plant Mol Biol, 2010, 72(6): 659-671

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Cloning and Expression Analysis of Circadian Clock Gene CCA1 in Populus

1. Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, Jiangsu, China

Received Date: 2013-05-29

Abstract: CCA1 gene plays an important role in circadian clock sensitivity in Populus. In this study, PtCCA1 was isolated from Populus tomentosa by RT-PCR. It has an open reading frame (ORF) of 1902 bp in length and encodes a protein of 663 amino acid residues, with protein molecular weight of 68.90 kDa and isoelectric point of 6.33. The amino acid sequence contained one Myb-like DNA binding domains and two putative transmembrane spanning regions. The results of fluorescence quantitative RT-PCR showed that the PtCCA1 gene was expressed mainly in leaf tissues. Along with the change of illumination time, the expression amount of PtCCA1 gene decreased during the day but gradually increased during the night. This study provides some references for further study of PtCCA1 function in regulating photoperiod sensitivity and the mechanism of circadian synchronization in poplars.

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Reference (23)

[1]	Michael T P, Salomé P A, Yu H J, et al. Enhanced fitness conferred by naturally occurring variation in the circadian clock [J]. Science , 2003,302(5647): 1049-1053
[2]	Dodd A N, Salathia N, Hall A, et al. Plant circadian clocks increase photosynthesis, growth, survival, and competitive advantage [J]. Science,2005, 309(5734): 630-633
[3]	Ni Z, Kim E D, Ha M, et al. Altered circadian rhythms regulate growth vigour in hybrids and allopolyploids [J]. Nature,2009, 457(7227): 327-331
[4]	Rund-Kangisser S, Yakir E, Green R. Proteasomal regulation of CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) stability is part of the complex control of CCA1[J]. Plant Signal Behav,2013, 8(3). pii: e23206
[5]	Gutierrez R A, Stokes T L, Thum K, et al. Systems approach identifies an organic nitrogen-responsive gene network that is regulated by the master clock control gene CCA1[J]. Proc Natl Acad Sci USA,2008,105(12): 4939-4944
[6]	Niwa Y, Ito S, Nakamichi N, et al. Genetic linkages of the circadian clock-associated genes, TOC1, CCA1 and LHY, in the photoperiodic control of flowering time in Arabidopsis thaliana[J]. Plant Cell Physiol,2007,48(7): 925-937
[7]	Kawamura M, Ito S, Nakamichi N,et al. The function of the clock-associated transcriptional regulator CCA1 (CIRCADIAN CLOCK-ASSOCIATED 1) in Arabidopsis thaliana[J]. Biosci Biotechnol Biochem,2008,72(5): 1307-1316
[8]	Alabadi D, Yanovsky M, Mas P, et al. Critical role for CCA1 and LHY in maintaining circadian rhythmicity in Arabidopsis[J]. Curr Biol,2002,12(9):757-761
[9]	Matsushika A, Makino S, Kojima M, et al. The APRR1/TOC1 quintet implicated in circadian rhythms of Arabidopsis thaliana: II. Characterization with CCA1-overexpressing plants[J]. Plant Cell Physiol,2002, 43(1): 118-122
[10]	邢国芳, 宋萌, 姚涵, 等. 大麦(Hordeum vulgare)昼夜节律钟基因CCA1的克隆及表达分析[J]. 山西农业大学学报,2012, 32(3): 207-212
[11]	邢国芳, 杜伟建, 张雁明, 等. 玉米昼夜节律钟基因CCA1的克隆及表达分析[J]. 山西农业大学学报,2011,31(4): 332-337
[12]	Tamura K, Peterson D, Peterson N, et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods [J]. Molecular Biology and Evolution,2011, 28(10): 2731-2739
[13]	Tuskan G. A, Difazio S, Jansson S,et al. The genome of black cottonwood, Populus trichocarpa (Torr. & Gray) [J]. Science,2006,313(5793): 1596-1604
[14]	Jansson S,Douglas C J. Populus: a model system for plant biology[J]. Annu Rev Plant Biol,2007, 58: 435-458
[15]	Daniel X, Sugano S, Tobin E M, et al. CK2 phosphorylation of CCA1 is necessary for its circadian oscillator function in Arabidopsis[J]. Proc Natl Acad Sci USA, 2004, 101(9): 3292-3297
[16]	Lu S X, Webb C J, Knowles S M, et al. CCA1 and ELF3 Interact in the control of hypocotyl length and flowering time in Arabidopsis[J]. Plant Physiol, 2012, 158(2): 1079-1088
[17]	Mizoguchi T, Wheatley K, Hanzawa Y, et al. LHY and CCA1 are partially redundant genes required to maintain circadian rhythms in Arabidopsis[J]. Dev Cell, 2002, 2(5): 629-641
[18]	Ito S, Nakamichi N, Nakamura Y, et al. Genetic linkages between circadian clock-associated components and phytochrome-dependent red light signal transduction in Arabidopsis thaliana[J]. Plant Cell Physiol, 2007, 48(7):971-983
[19]	Alabadi D, Oyama T, Yanovsky M, et al. Reciprocal regulation between TOCl and LHY/CCAl within the Arabidopsis circadian clock[J]. Science, 2001, 293(553I): 880-883
[20]	Kikis E A, Khanna R, Quail P H, et al. ELF4 is a phytochrome-regulated component of a negative-feedback loop involving the cntral oscillator components CCA1 and LHY[J]. Plant J, 2005, 44(2): 300-313
[21]	Fujimori T, Sato E, Yamashino Y, et al. PRR5 (PSEUDO-RESPONSE REGULATOR 5) plays antagonistic roles to CCA1 (CIRCADIAN CLOCK-ASSOCIATED 1) in Arabidopsis thaliana[J]. Biosci Biotechnol Biochem, 2005, 69(2): 426-430
[22]	Wang X, Wu L, Zhang S, et al. Robust expression and association of ZmCCA1 with circadian rhythms in maize [J]. Plant Cell Rep, 2011, 30(7): 1261-1272
[23]	Ovadia A, Tabibian-Keissar H, Cohen Y, et al. The 5'UTR of CCA1 includes an autoregulatory cis element that segregates between light and circadian regulation of CCA1 and LHY[J]. Plant Mol Biol, 2010, 72(6): 659-671

Cloning and Expression Analysis of Circadian Clock Gene CCA1 in Populus

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通讯作者: 陈斌, bchen63@163.com

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Cloning and Expression Analysis of Circadian Clock Gene CCA1 in Populus

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