Characterization and Expression of the Somatic Embryogenesis Receptor-like Kinase 1 (SERK1) Gene During Somatic Embryogenesis of Larix kaempferi

LI Long; ZHANG Li-feng; QI Li-wang; HAN Su-ying

Volume 26 Issue 6

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Characterization and Expression of the Somatic Embryogenesis Receptor-like Kinase 1 (SERK1) Gene During Somatic Embryogenesis of Larix kaempferi

1.
Research institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China
2.
Laboratory of Cell Biology, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Received Date: 2013-04-16

Abstract

Somatic embryogenesis not only plays a significant role in understanding the mechanism of embryogenesis, but also in improving the breeding of Larix kaempferi (Lamb.) Carr. The somatic embryogenesis receptor-like kinase 1 (SERK1) gene is considered as an essential element involved in the early stage of this process. The expression pattern of the homolog of SERK1 of L.kaempferi in different culture conditions was studied. LaSERK1 shared all the characteristic domains of the SERK family. The qRT-PCR showed that LaSERK1 was highly expressed in L.kaempferi early embryogenic cells cultured in the mature medium. These results suggest that LaSERK1 might perform a significant role during early somatic embryogenesis of L.kaempferi, and might be a potential marker of early embryogenic cells.
- Larix kaempferi,
- somatic embryogenesis,
- SERK1,
- expression pattern

References

[1]	Zhang S, Zhou J, Han S, et al. Four abiotic stress-induced miRNA families differentially regulated in the embryogenic and non-embryogenic callus tissues of Larix kaempferi (Lamb.) Carr.[J]. Biochemical and biophysical research communications, 2010, 398(3): 355-360
[2]	Cairney J, Pullman G S. The cellular and molecular biology of conifer embryogenesis[J]. New Phytologist, 2007, 176(3): 511-536
[3]	Schmidt E D, Guzzo F, Toonen M A, et al. A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos[J]. Development, 1997, 124(10): 2049-2062
[4]	Lotan T, Ohto M, Yee K M, et al. Arabidopsis LEAFY COTYLEDON1 Is Sufficient to Induce Embryo Development in Vegetative Cells[J]. Cell, 1998, 93(7): 1195-1205
[5]	Clark S E, Williams R W, Meyerowitz E M. The CLAVATA1 gene encodes a putative receptor kinase that controls shoot and floral meristem size in Arabidopsis[J]. Cell, 1997, 89(4): 575-585
[6]	Mayer K F X, Schoof H, Haecker A, et al. Role of WUSCHEL in Regulating Stem Cell Fate in the Arabidopsis Shoot Meristem[J]. Cell, 1998, 95(6): 805-815
[7]	Espelund M, Sbe-Larssen S, Hughes D W, et al. Late embryogenesis-abundant genes encoding proteins with different numbers of hydrophilic repeats are regulated differentially by abscisic acid and osmotic stress[J]. The Plant Journal, 1992, 2(2): 241-252
[8]	Boucheron E, Healy J H S, Bajon C, et al. Ectopic expression of Arabidopsis CYCD2 and CYCD3 in tobacco has distinct effects on the structural organization of the shoot apical meristem[J]. Journal of experimental botany, 2005, 56(409): 123-134
[9]	Kreuger M, van Holst G J. Arabinogalactan proteins and plant differentiation[J]. Plant molecular biology, 1996, 30(6): 1077-1086
[10]	Zhang X S, Choi J H, Heinz J, et al. Domain-specific positive selection contributes to the evolution of Arabidopsis leucine-rich repeat receptor-like kinase (LRR RLK) genes[J]. Journal of molecular evolution, 2006, 63(5): 612-621
[11]	Becraft P W. Receptor kinase signaling in plant development[J]. Annual review of cell and developmental biology, 2002, 18(1): 163-192
[12]	Hecht V, Vielle-Calzada J P, Hartog M V, et al. The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture[J]. Plant Physiology, 2001, 127(3): 803-816
[13]	Baudino S, Hansen S, Brettschneider R, et al. Molecular characterisation of two novel maize LRR receptor-like kinases, which belong to the SERK gene family[J]. Planta, 2001, 213(1): 1-10
[14]	Hu H, Xiong L, Yang Y. Rice SERK1 gene positively regulates somatic embryogenesis of cultured cell and host defense response against fungal infection[J]. Planta, 2005, 222(1): 107-117
[15]	Budiman M A, Mao L, Wood T C, et al. A deep-coverage tomato BAC library and prospects toward development of an STC framework for genome sequencing[J]. Genome research, 2000, 10(1): 129-136
[16]	de Oliveira Santos M, Romano E, Yotoko K S C, et al. Characterisation of the cacao somatic embryogenesis receptor-like kinase (SERK) gene expressed during somatic embryogenesis[J]. Plant Science, 2005, 168(3): 723-729
[17]	Thomas C, Meyer D, Himber C, et al. Spatial expression of a sunflower SERK gene during induction of somatic embryogenesis and shoot organogenesis[J]. Plant Physiology and Biochemistry, 2004, 42(1): 35-42
[18]	Nolan K E, Irwanto R R, Rose R J. Auxin up-regulates MtSERK1 expression in both Medicago truncatula root-forming and embryogenic cultures[J]. Plant Physiology, 2003, 133(1): 218-230
[19]	高燕, 席梦利, 王桂凤, 等. 马尾松体细胞胚胎发生相关基因 PmSERK1 的克隆与表达分析[J]. 分子植物育种, 2010 (1): 53-58
[20]	Steiner N, Santa-Catarina C, Guerra M P, et al. A gymnosperm homolog of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE-1 (SERK1)is expressed during somatic embryogenesis[J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2012, 109(1): 41-50
[21]	齐力旺. 华北落叶松体细胞胚胎发生及遗传转化实验系统的建立[J]. 实验生物学报, 2000, 33(4): 354-365
[22]	Ren J, Wen L, Gao X, et al. DOG 1.0: illustrator of protein domain structures[J]. Cell research, 2009, 19(2): 271-273
[23]	Arnold K, Bordoli L, Kopp J, et al. The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling[J]. Bioinformatics, 2006, 22(2): 195-201
[24]	Guex N, Peitsch M C. SWISS-MODEL and the Swiss-Pdb Viewer: An environment for comparative protein modeling[J]. Electrophoresis, 1997, 18(15): 2714-2723
[25]	Schwede T, Kopp J, Guex N, et al. SWISS-MODEL: an automated protein homology-modeling server[J]. Nucleic acids research, 2003, 31(13): 3381-3385
[26]	Kateřina S, Zuzana V, Lukáš F, et al. The role of actin isoforms in somatic embryogenesis in Norway spruce[J]. BMC Plant Biology, 2010, 10:89
[27]	Shimada T, Hirabayashi T, Endo T, et al. Isolation and characterization of the somatic embryogenesis receptor-like kinase gene homologue (CitSERK1) from Citrus unshiu Marc[J]. Scientia Horticulturae, 2005, 103(2): 233-238

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Characterization and Expression of the Somatic Embryogenesis Receptor-like Kinase 1 (SERK1) Gene During Somatic Embryogenesis of Larix kaempferi

1. Research institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China

2. Laboratory of Cell Biology, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Received Date: 2013-04-16

Abstract: Somatic embryogenesis not only plays a significant role in understanding the mechanism of embryogenesis, but also in improving the breeding of Larix kaempferi (Lamb.) Carr. The somatic embryogenesis receptor-like kinase 1 (SERK1) gene is considered as an essential element involved in the early stage of this process. The expression pattern of the homolog of SERK1 of L.kaempferi in different culture conditions was studied. LaSERK1 shared all the characteristic domains of the SERK family. The qRT-PCR showed that LaSERK1 was highly expressed in L.kaempferi early embryogenic cells cultured in the mature medium. These results suggest that LaSERK1 might perform a significant role during early somatic embryogenesis of L.kaempferi, and might be a potential marker of early embryogenic cells.

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Reference (27)

[1]	Zhang S, Zhou J, Han S, et al. Four abiotic stress-induced miRNA families differentially regulated in the embryogenic and non-embryogenic callus tissues of Larix kaempferi (Lamb.) Carr.[J]. Biochemical and biophysical research communications, 2010, 398(3): 355-360
[2]	Cairney J, Pullman G S. The cellular and molecular biology of conifer embryogenesis[J]. New Phytologist, 2007, 176(3): 511-536
[3]	Schmidt E D, Guzzo F, Toonen M A, et al. A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos[J]. Development, 1997, 124(10): 2049-2062
[4]	Lotan T, Ohto M, Yee K M, et al. Arabidopsis LEAFY COTYLEDON1 Is Sufficient to Induce Embryo Development in Vegetative Cells[J]. Cell, 1998, 93(7): 1195-1205
[5]	Clark S E, Williams R W, Meyerowitz E M. The CLAVATA1 gene encodes a putative receptor kinase that controls shoot and floral meristem size in Arabidopsis[J]. Cell, 1997, 89(4): 575-585
[6]	Mayer K F X, Schoof H, Haecker A, et al. Role of WUSCHEL in Regulating Stem Cell Fate in the Arabidopsis Shoot Meristem[J]. Cell, 1998, 95(6): 805-815
[7]	Espelund M, Sbe-Larssen S, Hughes D W, et al. Late embryogenesis-abundant genes encoding proteins with different numbers of hydrophilic repeats are regulated differentially by abscisic acid and osmotic stress[J]. The Plant Journal, 1992, 2(2): 241-252
[8]	Boucheron E, Healy J H S, Bajon C, et al. Ectopic expression of Arabidopsis CYCD2 and CYCD3 in tobacco has distinct effects on the structural organization of the shoot apical meristem[J]. Journal of experimental botany, 2005, 56(409): 123-134
[9]	Kreuger M, van Holst G J. Arabinogalactan proteins and plant differentiation[J]. Plant molecular biology, 1996, 30(6): 1077-1086
[10]	Zhang X S, Choi J H, Heinz J, et al. Domain-specific positive selection contributes to the evolution of Arabidopsis leucine-rich repeat receptor-like kinase (LRR RLK) genes[J]. Journal of molecular evolution, 2006, 63(5): 612-621
[11]	Becraft P W. Receptor kinase signaling in plant development[J]. Annual review of cell and developmental biology, 2002, 18(1): 163-192
[12]	Hecht V, Vielle-Calzada J P, Hartog M V, et al. The Arabidopsis SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture[J]. Plant Physiology, 2001, 127(3): 803-816
[13]	Baudino S, Hansen S, Brettschneider R, et al. Molecular characterisation of two novel maize LRR receptor-like kinases, which belong to the SERK gene family[J]. Planta, 2001, 213(1): 1-10
[14]	Hu H, Xiong L, Yang Y. Rice SERK1 gene positively regulates somatic embryogenesis of cultured cell and host defense response against fungal infection[J]. Planta, 2005, 222(1): 107-117
[15]	Budiman M A, Mao L, Wood T C, et al. A deep-coverage tomato BAC library and prospects toward development of an STC framework for genome sequencing[J]. Genome research, 2000, 10(1): 129-136
[16]	de Oliveira Santos M, Romano E, Yotoko K S C, et al. Characterisation of the cacao somatic embryogenesis receptor-like kinase (SERK) gene expressed during somatic embryogenesis[J]. Plant Science, 2005, 168(3): 723-729
[17]	Thomas C, Meyer D, Himber C, et al. Spatial expression of a sunflower SERK gene during induction of somatic embryogenesis and shoot organogenesis[J]. Plant Physiology and Biochemistry, 2004, 42(1): 35-42
[18]	Nolan K E, Irwanto R R, Rose R J. Auxin up-regulates MtSERK1 expression in both Medicago truncatula root-forming and embryogenic cultures[J]. Plant Physiology, 2003, 133(1): 218-230
[19]	高燕, 席梦利, 王桂凤, 等. 马尾松体细胞胚胎发生相关基因 PmSERK1 的克隆与表达分析[J]. 分子植物育种, 2010 (1): 53-58
[20]	Steiner N, Santa-Catarina C, Guerra M P, et al. A gymnosperm homolog of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE-1 (SERK1)is expressed during somatic embryogenesis[J]. Plant Cell, Tissue and Organ Culture (PCTOC), 2012, 109(1): 41-50
[21]	齐力旺. 华北落叶松体细胞胚胎发生及遗传转化实验系统的建立[J]. 实验生物学报, 2000, 33(4): 354-365
[22]	Ren J, Wen L, Gao X, et al. DOG 1.0: illustrator of protein domain structures[J]. Cell research, 2009, 19(2): 271-273
[23]	Arnold K, Bordoli L, Kopp J, et al. The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling[J]. Bioinformatics, 2006, 22(2): 195-201
[24]	Guex N, Peitsch M C. SWISS-MODEL and the Swiss-Pdb Viewer: An environment for comparative protein modeling[J]. Electrophoresis, 1997, 18(15): 2714-2723
[25]	Schwede T, Kopp J, Guex N, et al. SWISS-MODEL: an automated protein homology-modeling server[J]. Nucleic acids research, 2003, 31(13): 3381-3385
[26]	Kateřina S, Zuzana V, Lukáš F, et al. The role of actin isoforms in somatic embryogenesis in Norway spruce[J]. BMC Plant Biology, 2010, 10:89
[27]	Shimada T, Hirabayashi T, Endo T, et al. Isolation and characterization of the somatic embryogenesis receptor-like kinase gene homologue (CitSERK1) from Citrus unshiu Marc[J]. Scientia Horticulturae, 2005, 103(2): 233-238

Characterization and Expression of the Somatic Embryogenesis Receptor-like Kinase 1 (SERK1) Gene During Somatic Embryogenesis of Larix kaempferi

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通讯作者: 陈斌, bchen63@163.com

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Characterization and Expression of the Somatic Embryogenesis Receptor-like Kinase 1 (SERK1) Gene During Somatic Embryogenesis of Larix kaempferi

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