• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

Citation:

Expression Analysis of Heat Shock Protein Genes in Ericerus pela under Cold Stress

  • Received Date: 2013-05-05
  • The strong tolerance to low temperatures is critical to the survival, reproduction and wax production of Ericerus pela. To investigate the effects of cold stress on heat shock protein (hsp) expression in Ericerus pela, the transcriptional expression pattern of 7 hsps (hsp21.5, hsp21.7, hsp40, hsp60, hsp70, hsc70, and hsp90) in second-instar male larvae was examined by quantitative real time PCR (qRT-PCR). The results showed that all the hsps were inducible by cold stress except hsc70. The expression of hsp21.7, hsp70 and hsp90 were drastically up-regulated. The results indicated that many hsps correlate with cold-stress tolerance in Ericerus pela.
  • 加载中
  • [1]

    Gething M J, Sambrook J. Protein folding in the cell[J]. Nature, 1992, 355: 33-45
    [2]

    Planelló R, Herrero O, Martínez-Guitarte J L, et al. Comparative effects of butyl benzyl phthalate (BBP) and di (2-ethylhexyl) phthalate (DEHP) on the aquatic larvae of Chironomus riparius based on gene expression assays related to the endocrine system, the stress response and ribosomes[J]. Aquatic Toxicology, 2011, 105: 62-70
    [3]

    Zhang Z, Zhang Q. Molecular cloning, characterization and expression of heat shock protein 70 gene from the oyster Crassostrea hongkongensis responding to thermal stress and exposure of Cu2+ and malachite green[J]. Gene, 2012, 497: 172-180
    [4]

    Sang W, Ma W H, Qiu L, et al. The involvement of heat shock protein and cytochrome P450 genes in response to UV-A exposure in the beetle Tribolium castaneum[J]. Journal of Insect Physiology, 2012, 58: 830-836
    [5]

    Burke G R, Moran N A. Responses of the pea aphid transcriptome to infection by facultative symbionts[J]. Insect Molecular Biology, 2011, 20: 357-365
    [6]

    Kanagasabai R, Krishnamurthy K, Druhan L J, et al. Forced expression of heat shock protein 27 (Hsp27) reverses P-glycoprotein (ABCB1)-mediated drug efflux and MDR1 gene expression in Adriamycin-resistant human breast cancer cells[J]. Journal of Biological Chemistry, 2011, 286: 33289-33300
    [7]

    Baruah K, Norouzitallab P, Shihao L, et al. Feeding truncated heat shock protein 70s protect Artemia franciscana against virulent Vibrio campbellii challenge[J]. Fish & Shellfish Immunology, 2013, 34(1): 183-191
    [8]

    Tamayo D, Muoz J F, Torres I, et al. Involvement of the 90 kDa heat shock protein during adaptation of Paracoccidioides brasiliensis to different environmental conditions[J]. Fungal Genetics and Biology, 2013, 51: 34-41
    [9] 王志明, 许晓明. 长春市一新外来入侵物种—白蜡虫的生物学特性与种群控制研究[J]. 吉林农业大学学报, 2006, 28(2): 152-154

    [10]

    Lalouette L, Williams C, Hervant F, et al. Metabolic rate and oxidative stress in insects exposed to low temperature thermal fluctuations[J]. Comparative Biochemistry and Physiology-Part A: Molecular & Integrative Physiology, 2011, 158: 229-234
    [11]

    Dunning L T, Dennis A B, Park D C, et al. Identification of cold-responsive genes in a New Zealand alpine stick insect using RNA-Seq[J]. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 2012, 8: 24-31
    [12] 焦 懿, 赵 萍. 白蜡虫泌蜡期天敌群落的研究[J]. 生态学报, 1999, 19(5): 732-736

    [13] 陈晓鸣, 王自力, 陈 勇, 等. 白蜡虫同地产虫产蜡生产模式构建与分析[J]. 林业科学, 2010, 12: 106-113

    [14]

    Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 2001, 25: 402-408
    [15] 唐启义, 冯明光. DPS数据处理系统-实验设计、统计分析及数据挖掘[M]. 北京: 科学出版社, 2010:82-84

    [16]

    Huang L H, Chen B, Kang L. Impact of mild temperature hardening on thermotolerance, fecundity, and Hsp gene expression in Liriomyza huidobrensis[J]. Journal of Insect Physiology, 2007a, 53(2): 1199-1205
    [17]

    Huang L H, Kang L. Cloning and interspecific altered expression of heat shock protein genes in two leafminer species in response to thermal stress[J]. Insect Molecular Biology, 2007b, 16(4): 491-500
    [18] 吕志创. 高温热激下雌雄B型烟粉虱差异表达基因的筛选和Hsps基因功能的鉴定[D].北京:中国农业科学院, 2008

    [19]

    Zou Z W, Sun Z X, Li J F, et al. Molecular cloning and characterization of two heat shock proteins in Thitarodes pui (Lepidoptera: Hepialidae)[J]. Cryoletters, 2011, 32(3): 225-239
    [20] Liu W W, Yang P, Chen X M, et al. Cloning and expression analysis of four heat shock protein genes in the Chinese white wax scale insect, Ericerus pela[J]. Journal of Insect science, 2013, (待发表)

    [21]

    Kostal V, Tollarova-Borovanska M. The 70 kDa heat shock protein assists during the repair of chilling injury in the insect, Pyrrhocoris apterus[J]. PLoS ONE, 2009, 4(2): e4546
    [22]

    Gusev O, Cornette R, Kikawada T, et al. Expression of heat shock protein-coding genes associated with anhydrobiosis in an African chironomid Polypedilum vanderplanki[J]. Cell Stress Chaperones, 2011, 16: 81-90
    [23]

    Zhang Q R, Denlinger D L. Molecular characterization of heat shock protein 90, 70 and 70 cognate cDNAs and their expression patterns during thermal stress and pupal diapause in the corn earworm[J]. Journal of Insect Physiology, 2010, 56(2): 138-150
    [24]

    Colinet H, Lee S F, Hoffmann A. Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster[J]. FEBS Journal, 2010, 277: 174-185
    [25]

    Sonoda S, Fukumoto K, Izumi Y, et al. Cloning of heat shock protein genes (hsp90 and hsc70) and their expression during larval diapause and cold tolerance acquisition in the rice stem borer, Chilo suppressalis Walker[J]. Archives of Insect Biochemistry and Physiology, 2006, 63(1): 36-47
    [26]

    Li Z W, Li X, Yu Q Y, et al. The small heat shock protein (sHSP) genes in the silkworm, Bombyx mori, and comparative analysis with other insect sHSP genes[J]. BMC Evolutionary Biology, 2009, 9: 215-224
    [27]

    Sakano D, Li B, Xia Q, et al. Genes encoding small heat shock proteins of the silkworm, Bombyx mori[J]. Bioscience, Biotechnology, and Biochemistry, 2006, 70(10): 2443-2450
    [28]

    Cui Y D, Du1a Y Z, Lu M X, et al. Cloning of the heat shock protein 60 gene from the stem borer, Chilo suppressalis, and analysis of expression characteristics under heat stress[J]. Journal of Insect Science, 2010, 10: 1-13
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article views(2916) PDF downloads(1163) Cited by()

Proportional views

Expression Analysis of Heat Shock Protein Genes in Ericerus pela under Cold Stress

  • 1. Research Institute of Resource Insects, Chinese Academy of Forestry

Abstract: The strong tolerance to low temperatures is critical to the survival, reproduction and wax production of Ericerus pela. To investigate the effects of cold stress on heat shock protein (hsp) expression in Ericerus pela, the transcriptional expression pattern of 7 hsps (hsp21.5, hsp21.7, hsp40, hsp60, hsp70, hsc70, and hsp90) in second-instar male larvae was examined by quantitative real time PCR (qRT-PCR). The results showed that all the hsps were inducible by cold stress except hsc70. The expression of hsp21.7, hsp70 and hsp90 were drastically up-regulated. The results indicated that many hsps correlate with cold-stress tolerance in Ericerus pela.

Reference (28)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return