Genetic Diversity on Walnut Cultivars in Xinjiang Revealed by FISH-AFLP

HE Jian; MA Qing-guo; Xuelaiti&#183;YASIN; SUN Dong; SUN Shi-guo; PEI Dong

In order to view the genetic basis of Xinjiang walnut germplasm resourses, the genetic diversity of 30 walnut cultivars (Juglans regia L.) authorized or approved by Xinjiang Uygur Autonomous Region Forest Tree Cultivar Registration Committee were investigated by using FISH-AFLP. Eight pairs of EcoRⅠ+3/MseⅠ+3 primer combinations were used to amplify the genomic DNA. 981 out of totally 1011 AFLP bands were polymorphic and the average percent of polymorphic bands (PPB) was 97.5%. The value of effective number of alleles (Ne) estimated by different primer combinations ranged from 1.188 7 to 1.234 7 with an average of 1.208 5; Nei's gene diversity (H) ranged from 0.118 3 to 0.141 2 with an average of 0.129 7 and Shannon's information index (I) ranged from 0.184 6 to 0.225 8 with an average of 0.206 6, which revealed a moderate level of genetic diversity. Unique fingerprintings for 30 walnut cultivars were established by 8 primer combinations in the research.

1. Kashi Forestry Technology Extension Station, Kashi 844000, Xinjiang, China

2. Research Institute of Forestry, Chinese Academy of Forestry

3. State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China

Received Date: 2012-11-08

Abstract: In order to view the genetic basis of Xinjiang walnut germplasm resourses, the genetic diversity of 30 walnut cultivars (Juglans regia L.) authorized or approved by Xinjiang Uygur Autonomous Region Forest Tree Cultivar Registration Committee were investigated by using FISH-AFLP. Eight pairs of EcoRⅠ+3/MseⅠ+3 primer combinations were used to amplify the genomic DNA. 981 out of totally 1011 AFLP bands were polymorphic and the average percent of polymorphic bands (PPB) was 97.5%. The value of effective number of alleles (Ne) estimated by different primer combinations ranged from 1.188 7 to 1.234 7 with an average of 1.208 5; Nei's gene diversity (H) ranged from 0.118 3 to 0.141 2 with an average of 0.129 7 and Shannon's information index (I) ranged from 0.184 6 to 0.225 8 with an average of 0.206 6, which revealed a moderate level of genetic diversity. Unique fingerprintings for 30 walnut cultivars were established by 8 primer combinations in the research.

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Reference (23)

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Genetic Diversity on Walnut Cultivars in Xinjiang Revealed by FISH-AFLP

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