Characterization of a Novel Ubiquitin-conjugating Enzyme from Camellia oleifera

LIN Ping; YAO Xiao-hua; CAO Yong-qing; LONG Wei; WANG Kai-liang; TENG Jian-hua

Volume 26 Issue 6

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Characterization of a Novel Ubiquitin-conjugating Enzyme from Camellia oleifera

1.
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China
2.
Dongfanghong Forest Farm of Jinhua City, Zhejiang Province, Jinhua 321025, Zhejiang, China

Received Date: 2012-09-26

Abstract

Ubiquitin-conjugating enzyme (E2) is one of three key enzymes in the ubiquitin-proteasome pathway (UPP). And it's very important in the protein degradation pathways depend on ubiquitin or N-end rule, including identify the target protein, interaction with ubiquitin-protein ligating enzymes (E3), and so on. A full length cDNA sequence of E2 was cloned by Solexa sequencing technology and named UBE2-J2. This cDNA codes 239 amino acids, and has significant amino acid sequence identity and similarity with E2s from other organism. The UBE2-J2 protein has an ubiquitin-conjugating enzyme E2 catalytic (UBCc) domain from 8th to 122nd amino acid residue. There are 17 residues to compose Ubiquitin thioester intermediate interaction residues, 5 residues to compose E3 interaction residues and the 87th residue is the active site cysteine on conserved domain UBCc. UBE2-J2 is a class Ⅱ member of E2 family of Camellia oleifera according to bioinformatics analysis.
- Camellia oleifera,
- ubiquitin-conjugating enzyme (E2),
- Solexa sequencing,
- bioinformatics

References

[1]	郭启芳, 邹琦, 王玮. 植物泛素/26S蛋白酶体通路的生理功能和分子生物学[J]. 植物生理学通讯, 2004, 40 ( 5): 533-539
[2]	朱经春, 王台, 陈克成. 泛肽系统的组成和功能(一)——系统组成、底物识别与蛋白质泛肽化[J]. 植物学通报, 1999, 16(3): 208-218
[3]	董发才, 宋纯鹏. 植物细胞中的泛素及其生理功能[J]. 植物生理学通讯, 1999, 35(1): 54-59
[4]	盛仙永, 胡正海, 林金星. 泛素/蛋白酶体途径及其在高等植物有性生殖中的作用[J]. 西北植物学报, 2004, 24( 8): 1527-1536
[5]	Ingvardsen C, Veierskov B. Ubiquitin and proteasome-dependent proteolysis in plants[J]. Physiologia Plantarum, 2001, 112(4): 451-459
[6]	Moon J, Parry G, Estelle M. The ubiquitin-proteasome pathway and plant development[J]. Plant Cell, 2004, 16(12): 3181-3195
[7]	Smalle J, Vierstra R D. The ubiquitin 26S proteasome proteolytic pathway[J]. Annual Review of Plant Biology, 2004, 55: 555-590
[8]	Dreher K, Callis J. Ubiquitin, hormones and biotic stress in plants[J]. Annals of Botany, 2007, 99(5): 787-822
[9]	Zhang Y, Yang C, Li Y, et al. SDIR1 is a RING finger E3 ligase that positively regulates stress-responsive abscisic acid signaling in Arabidopsis[J]. Plant Cell, 2007, 19(6): 1912-1929
[10]	Shi S Q, Shi Z, Jiang Z P, et al. Effects of exogenous GABA on gene expression of Caragana intermedia roots under NaCl stress: regulatory roles for H₂O₂ and ethylene production[J]. Plant, Cell & Environment, 2009, 33(2):149-162
[11]	王金利, 史胜青, 贾利强, 等. 植物泛素结合酶E2功能研究进展[J]. 生物技术通报, 2010(4): 7-10
[12]	徐晨曦, 姜静, 刘甜甜, 等. 柽柳泛素结合酶基因(E2s)的序列分析及功能验证[J]. 东北林业大学学报, 2007, 35(11): 1-4
[13]	黄海杰, 陈雄庭. 植物泛素/26S蛋白酶体途径研究进展[J]. 中国生物工程杂志, 2008, 28(7): 127-132
[14]	许传俊, 李玲. 泛素/26S 蛋白酶体途径与植物的生长发育[J]. 西北植物学报, 2007, 27(3): 635-643
[15]	Gohre V, Spallek T, Haweker H, et al. Plant pattern-recognition receptor FLS2 is directed for degradation by the bacterial ubiquitin ligase AvrPtoB[J]. Current Biology, 2008, 18 (23): 1824-1832
[16]	Lai J, Chen H, Teng K, et al. RKP, a RING finger E3 ligase induced by BSCTV C4 protein, affects geminivirus in fection by regulation of he plant cell cycle[J]. Plant Journal, 2009, 57(5): 905-917
[17]	Lee H K, Cho S K, Son O S, et al. Drought stress-induced Rma1H1, a RING membrane-anchor E3 ubiquitin ligase homolog, regulates aquaporin levels via ubiquitination in transgenic Arabidopsis plants[J]. Plant Cell, 2009, 21 (2): 622-641
[18]	Pan J, Fujioka S, Peng J, et al. The E3 ubiquitin ligase SCFTIR1/AFB and membrane sterols play key roles in auxin regulation of endocytosis, recycling, and plasma membrane accumulation of the auxin efflux transporter PIN2 in Arabidopsis thaliana[J]. Plant Cell, 2009, 21(2): 568-580
[19]	郭静怡. 油茶泛素结合酶E2基因全长cDNA克隆及其RNAi干扰载体的构建[D]. 长沙: 中南林业科技大学, 2011
[20]	Mortazavi A, Williams B A, McCue K, et al. Mapping and quantifying mammalian transcriptomes by RNA-Seq[J]. Nature Methods, 2008, 5(7): 621-628
[21]	Altschul S F, Madden T L, Schaffer A A, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs[J]. Nucleic Acids Research, 1997, 25(17): 3389-3402
[22]	de Castro E, Sigrist C J, Gattiker A, et al. ScanProsite: detection of PROSITE signature matches and ProRule-associated functional and structural residues in proteins[J]. Nucleic Acids Research, 2006, 34(web server issue): 362-365
[23]	Marchler-bauer A, Anderson J B, Cherukuri P F, et al. CDD: a Conserved Domain Database for protein classification[J]. Nucleic Acids Research, 2005, 33(database issue): 192-196
[24]	易乐飞, 刘楚吾, 王萍, 等. 条斑紫菜泛素结合酶基因的cDNA序列克隆与分析[J]. 水产学报, 2009, 33(5): 719-726
[25]	Schwede T, Kopp J, Guex N, et al. SWISS-MODEL: an automated protein homology-modeling server[J]. Nucleic Acids Research, 2003, 31(13): 3381-3385
[26]	陈建明, 余应年. 真核泛素缀合途径研究进展[J]. 中国病理生理杂志, 2000, 16(2): 175-178
[27]	Plafker S M, Plafker K S, Weissman A M, et al. Ubiquitin charging of human class Ⅲ ubqintin-conjugating enzymes triggers their nuclear import[J]. Journal of Cell Biology, 2004, 167(4): 649-659
[28]	Matuschewski K, Hauser H P, Treier M, et al. Identification of a novel family of ubqintin-conjugating enzymes with distinct amino-terminal extensions[J]. Journal of Biological Chemistry, 1996, 271(5): 2789-2794
[29]	Jentsch S. The ubiquitin-conjugation system[J]. Annual Review of Genetics, 1992, 26: 179-207
[30]	赖燕, 徐波, 林明, 等. 辣椒泛素交联酶cDNA的分离及其在UV-B作用下的表达分析[J]. 福建农林大学学报: 自然科学版, 2008, 37(2): 162-165

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Characterization of a Novel Ubiquitin-conjugating Enzyme from Camellia oleifera

1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China

2. Dongfanghong Forest Farm of Jinhua City, Zhejiang Province, Jinhua 321025, Zhejiang, China

Received Date: 2012-09-26

Abstract: Ubiquitin-conjugating enzyme (E2) is one of three key enzymes in the ubiquitin-proteasome pathway (UPP). And it's very important in the protein degradation pathways depend on ubiquitin or N-end rule, including identify the target protein, interaction with ubiquitin-protein ligating enzymes (E3), and so on. A full length cDNA sequence of E2 was cloned by Solexa sequencing technology and named UBE2-J2. This cDNA codes 239 amino acids, and has significant amino acid sequence identity and similarity with E2s from other organism. The UBE2-J2 protein has an ubiquitin-conjugating enzyme E2 catalytic (UBCc) domain from 8th to 122nd amino acid residue. There are 17 residues to compose Ubiquitin thioester intermediate interaction residues, 5 residues to compose E3 interaction residues and the 87th residue is the active site cysteine on conserved domain UBCc. UBE2-J2 is a class Ⅱ member of E2 family of Camellia oleifera according to bioinformatics analysis.

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Reference (30)

[1]	郭启芳, 邹琦, 王玮. 植物泛素/26S蛋白酶体通路的生理功能和分子生物学[J]. 植物生理学通讯, 2004, 40 ( 5): 533-539
[2]	朱经春, 王台, 陈克成. 泛肽系统的组成和功能(一)——系统组成、底物识别与蛋白质泛肽化[J]. 植物学通报, 1999, 16(3): 208-218
[3]	董发才, 宋纯鹏. 植物细胞中的泛素及其生理功能[J]. 植物生理学通讯, 1999, 35(1): 54-59
[4]	盛仙永, 胡正海, 林金星. 泛素/蛋白酶体途径及其在高等植物有性生殖中的作用[J]. 西北植物学报, 2004, 24( 8): 1527-1536
[5]	Ingvardsen C, Veierskov B. Ubiquitin and proteasome-dependent proteolysis in plants[J]. Physiologia Plantarum, 2001, 112(4): 451-459
[6]	Moon J, Parry G, Estelle M. The ubiquitin-proteasome pathway and plant development[J]. Plant Cell, 2004, 16(12): 3181-3195
[7]	Smalle J, Vierstra R D. The ubiquitin 26S proteasome proteolytic pathway[J]. Annual Review of Plant Biology, 2004, 55: 555-590
[8]	Dreher K, Callis J. Ubiquitin, hormones and biotic stress in plants[J]. Annals of Botany, 2007, 99(5): 787-822
[9]	Zhang Y, Yang C, Li Y, et al. SDIR1 is a RING finger E3 ligase that positively regulates stress-responsive abscisic acid signaling in Arabidopsis[J]. Plant Cell, 2007, 19(6): 1912-1929
[10]	Shi S Q, Shi Z, Jiang Z P, et al. Effects of exogenous GABA on gene expression of Caragana intermedia roots under NaCl stress: regulatory roles for H₂O₂ and ethylene production[J]. Plant, Cell & Environment, 2009, 33(2):149-162
[11]	王金利, 史胜青, 贾利强, 等. 植物泛素结合酶E2功能研究进展[J]. 生物技术通报, 2010(4): 7-10
[12]	徐晨曦, 姜静, 刘甜甜, 等. 柽柳泛素结合酶基因(E2s)的序列分析及功能验证[J]. 东北林业大学学报, 2007, 35(11): 1-4
[13]	黄海杰, 陈雄庭. 植物泛素/26S蛋白酶体途径研究进展[J]. 中国生物工程杂志, 2008, 28(7): 127-132
[14]	许传俊, 李玲. 泛素/26S 蛋白酶体途径与植物的生长发育[J]. 西北植物学报, 2007, 27(3): 635-643
[15]	Gohre V, Spallek T, Haweker H, et al. Plant pattern-recognition receptor FLS2 is directed for degradation by the bacterial ubiquitin ligase AvrPtoB[J]. Current Biology, 2008, 18 (23): 1824-1832
[16]	Lai J, Chen H, Teng K, et al. RKP, a RING finger E3 ligase induced by BSCTV C4 protein, affects geminivirus in fection by regulation of he plant cell cycle[J]. Plant Journal, 2009, 57(5): 905-917
[17]	Lee H K, Cho S K, Son O S, et al. Drought stress-induced Rma1H1, a RING membrane-anchor E3 ubiquitin ligase homolog, regulates aquaporin levels via ubiquitination in transgenic Arabidopsis plants[J]. Plant Cell, 2009, 21 (2): 622-641
[18]	Pan J, Fujioka S, Peng J, et al. The E3 ubiquitin ligase SCFTIR1/AFB and membrane sterols play key roles in auxin regulation of endocytosis, recycling, and plasma membrane accumulation of the auxin efflux transporter PIN2 in Arabidopsis thaliana[J]. Plant Cell, 2009, 21(2): 568-580
[19]	郭静怡. 油茶泛素结合酶E2基因全长cDNA克隆及其RNAi干扰载体的构建[D]. 长沙: 中南林业科技大学, 2011
[20]	Mortazavi A, Williams B A, McCue K, et al. Mapping and quantifying mammalian transcriptomes by RNA-Seq[J]. Nature Methods, 2008, 5(7): 621-628
[21]	Altschul S F, Madden T L, Schaffer A A, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs[J]. Nucleic Acids Research, 1997, 25(17): 3389-3402
[22]	de Castro E, Sigrist C J, Gattiker A, et al. ScanProsite: detection of PROSITE signature matches and ProRule-associated functional and structural residues in proteins[J]. Nucleic Acids Research, 2006, 34(web server issue): 362-365
[23]	Marchler-bauer A, Anderson J B, Cherukuri P F, et al. CDD: a Conserved Domain Database for protein classification[J]. Nucleic Acids Research, 2005, 33(database issue): 192-196
[24]	易乐飞, 刘楚吾, 王萍, 等. 条斑紫菜泛素结合酶基因的cDNA序列克隆与分析[J]. 水产学报, 2009, 33(5): 719-726
[25]	Schwede T, Kopp J, Guex N, et al. SWISS-MODEL: an automated protein homology-modeling server[J]. Nucleic Acids Research, 2003, 31(13): 3381-3385
[26]	陈建明, 余应年. 真核泛素缀合途径研究进展[J]. 中国病理生理杂志, 2000, 16(2): 175-178
[27]	Plafker S M, Plafker K S, Weissman A M, et al. Ubiquitin charging of human class Ⅲ ubqintin-conjugating enzymes triggers their nuclear import[J]. Journal of Cell Biology, 2004, 167(4): 649-659
[28]	Matuschewski K, Hauser H P, Treier M, et al. Identification of a novel family of ubqintin-conjugating enzymes with distinct amino-terminal extensions[J]. Journal of Biological Chemistry, 1996, 271(5): 2789-2794
[29]	Jentsch S. The ubiquitin-conjugation system[J]. Annual Review of Genetics, 1992, 26: 179-207
[30]	赖燕, 徐波, 林明, 等. 辣椒泛素交联酶cDNA的分离及其在UV-B作用下的表达分析[J]. 福建农林大学学报: 自然科学版, 2008, 37(2): 162-165

Characterization of a Novel Ubiquitin-conjugating Enzyme from Camellia oleifera

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通讯作者: 陈斌, bchen63@163.com

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Characterization of a Novel Ubiquitin-conjugating Enzyme from Camellia oleifera

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