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Study on the Difference in Expression Profiles of microRNA Among Leaf Organs of Triploid and Diploid Populus×euramericana cl.‘Zhonglin46’

  • Received Date: 2013-08-20
  • Using total RNA extracted by CTAB method as templates, the expression profiles of 5 miRNAs in young and mature leaf organs of Populus×euramericana cl.‘Zhonglin46’were studied by real-time stem-loop RT-PCR. The results showed that the expression of miR159, miR167, miR171, and miR319 in triploid young leaves were higher than those in diploid, however, the expression of miR164 in mature leaves of triploid was higher than that in diploid. In addition, the expression analysis of the corresponding miRNA target genes showed that the expression of the miRNAs and their corresponding targets were negative correlation in diploid and triploid P.×euramericana cl.‘Zhonglin46’. these findings suggested that these miRNA target genes may be related to the formation of fast-growing traits showed by triploid Populus.
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  • [1]

    Lee R C,Feinbaum R L, Ambros V.The C. elegans heterochronic gene lin- 4 enc-odes small RNAs with antisense complementarity to lin- 14 [J].Cell, 1993, 75(5):843-854
    [2]

    Wightman B,Ha I,Ruvkun G.Posttranscriptional regulation of the heterochronic gene lin- 14 by lin- 4 mediates temporal pattern formation in C. elegans [J].Cell,1993,75(5):855-862
    [3]

    Reinhart B J,Weinstein E G,Rhoades M W, et al. MicroRNAs in plants [J].Genes & Development,2002,16(13):1616-1626
    [4]

    Schwab R, Palatnik J F, Riester M, et al. Specific effects of microRNAs on the plant transcriptome [J]. Developmental Cell, 2005, 8(4):517-527
    [5]

    Jacobsen S E, Running M P, Meyerowitz E M. Disruption of an RNA helicase/RNAse Ⅲ gene in Arabidopsis causes unregulated cell division in floral meristems [J]. Development, 1999, 126(23):5231-5243
    [6]

    Wang J W, Wang L J, Mao Y B, et al. Control of root cap formation by MicroRNA-targeted auxin response factors in Arabidopsis[J]. Plant Cell, 2005, 17(8):2204-2216
    [7]

    Williams L, Grigg S P, Xie M, et al. Regulation of Arabidopsis shoot apical meristem and lateral organ formation by microRNA miR166g and its AtHD-ZIP target genes [J]. Development, 2005, 132(16):3657-3668
    [8] 陈成彬, 齐力旺, 张守攻,等. 三倍体杨树核型分析[J]. 武汉植物学研究,2004, 22(6):565-567

    [9]

    Chen C F, Ridzon D A, Brootner A J, et al. Real-time quantification of microRNAs by stem-loop RT-PCR [J]. Nucleic Acids Research, 2005, 33(20):179-187
    [10]

    Kiefer E, Heller W, Ernst D. A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites [J]. Plant Mol Biol Rep, 2000, 18(1): 33-39
    [11]

    Palatnik J F,Allen E.Control of leaf morphogenesis by miRNAs[J].Nature,2003, 425(6955):257-263
    [12]

    Allen R S, Li J, Stable M I, et al. Genetic analysis reveals functional redundancy and the major target genes of the Arabidopsis miR159 family [J]. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(41):16371-16376
    [13]

    Bonnet E, Wuyts J, Rouzé P, et al. Detection of 91 potential conserved plant microRNAs in Arabidopsis thaliana and Oryza sativa identifies important target genes [J]. Proceedings of the National Academy of Sciences of the United States of America,2004, 101(31): 11511-11516
    [14]

    Guo M,Davis D,Birchler J A.Dosage effects on gene expression in a maize ploidy series[J].Genetics, 1996,142(4):1349-1355
    [15]

    Tariq M,Paszkowski,J.DNA and histone methylation in plants [J].Trends in Genetics, 2004, 20(6):244-251
    [16]

    Nath U,Crawford B C,Carpenter R, et al.Genetic control of surface curvature [J].Science,2003, 299(5611):1404-1407
    [17]

    Palatnik J F,Allen E,Wu X, et al.Control of leaf morphogenesis by microRNAs [J].Nature,2003, 425(6955):257-263
    [18]

    Ori N,Cohen A R,Etzioni A, et al.Regulation of LANCEOLATE by miR319 is required for compound-leaf development in tomato[J].Nature Genetics, 2007, 39(6):787-791
    [19]

    Masterson J.Stomatal size in fossil plants: evidence for polyploidy in majority of angiosperms[J].Science, 1994, 264(5157):421-424
    [20]

    Chen Z J,Ni Z.Mechanisms of genomic rearrangements and gene expression changes in plant polyploids[J].Bioessays, 2006, 28(3):240-252
    [21] 谭建新,孙玉洁. 表观基因组学研究方法进展与评价[J].遗传, 2009, 31(1):7-16

    [22]

    Kim J, Woo H, Kim J, et al. Trifurcate feed-forward regulation of age-dependent cell death involving miR164 in Arabidopsis[J]. Science, 2009, 323(5917):1053-1057
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Study on the Difference in Expression Profiles of microRNA Among Leaf Organs of Triploid and Diploid Populus×euramericana cl.‘Zhonglin46’

  • 1. College of Life Sciences, Nankai University, Tianjin 300071, China

Abstract: Using total RNA extracted by CTAB method as templates, the expression profiles of 5 miRNAs in young and mature leaf organs of Populus×euramericana cl.‘Zhonglin46’were studied by real-time stem-loop RT-PCR. The results showed that the expression of miR159, miR167, miR171, and miR319 in triploid young leaves were higher than those in diploid, however, the expression of miR164 in mature leaves of triploid was higher than that in diploid. In addition, the expression analysis of the corresponding miRNA target genes showed that the expression of the miRNAs and their corresponding targets were negative correlation in diploid and triploid P.×euramericana cl.‘Zhonglin46’. these findings suggested that these miRNA target genes may be related to the formation of fast-growing traits showed by triploid Populus.

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