Molecular Cloning and Function Analysis of PtSIP3 Gene from Populus&#215;euramericana cl.‘Zhonglin 46’Triploid

HU Bao-quan; WEI Tao; WANG Chun-guo; SONG Wen-qin; CHEN Cheng-bin

Volume 26 Issue S1

Article Contents

Turn off MathJax

Article Navigation > Forest Research > 2013 > 26(S1): 39-44

Citation:

Molecular Cloning and Function Analysis of PtSIP3 Gene from Populus×euramericana cl.‘Zhonglin 46’Triploid

1.
College of Life Sciences, Nankai University, Tianjin 300071, China

Received Date: 2013-08-22

Abstract

According to the preliminary results of microarray analysis, a high expression gene, PtSIP3, was cloned from Populus euramericana triploid, which generated from crosses between the maternal parent P. deltoides and the pollen parent P. nigra. The binary expression vector, pRI101- PtSIP3, was constructed and transformed into Arabidopsis. So far, the PtSIP3 transgenic plants were observed the remarkable phenotypic changes with inhibition of embryo and capsule development, improvement root development. Therefore, It is speculated that the rises of PtSIP3 expression may participate in the regulation of vegetative growth and promote root development in P. euramericana triploid.
- Populus euramericana,
- triploid,
- PtSIP3,
- gene expression

References

[1]	Stettler R, Zsuffa L, R.Wu. The role of hybridization in the genetic manipulation of Populus [J]. Biology of Populus and Its Implications for Management and Conservation (Part Ⅰ), 1996, 87-112
[2]	李善文, 张志毅, 何承忠. 中国杨树杂交育种研究进展[J]. 世界林业研究, 2004, 17(2): 37-41
[3]	Bradshaw Jr H, Stettler R. Stettler. Molecular genetics of growth and development in Populus. I. Triploidy in hybrid poplars[J]. Theoretical and Applied Genetics, 1993, 86(2-3): 301-307
[4]	Li Y, Kang X, Wang S, et al. Triploid induction in Populus alba x P. glandulosa by chromosome doubling of female gametes [J]. Silvae Genetica, 2008, 57(1): 37
[5]	Wu, R. Quantitative genetic variation of leaf size and shape in a mixed diploid and triploid population of Populus[J]. Genetical research, 2000, 75(2): 215-222
[6]	Sederoff, R. Regulatory science in forest biotechnology[J]. Tree Genetics & Genomes, 2007, 3(2): 71-74
[7]	Tuskan G A, Difazio S, Jansson S, et al. The genome of black cottonwood, Populus trichocarpa (Torr. & Gray) [J]. Science, 2006, 313(5793): 1596-1604
[8]	Tripathi V, Syed N, Laxmi A, et al. Role of CIPK6 in root growth and auxin transport [J]. Plant Signaling & Behavior, 2009, 4(7): 663-665
[9]	Kim M C, Chung W S, Yun D J, et al. Calcium and calmodulin-mediated regulation of gene expression in plants [J]. Molecular plant, 2009, 2(1): 13-21
[10]	Liu J, Zhu J K. A calcium sensor homolog required for plant salt tolerance [J]. Science, 1998, 280(5371): 1943-1945
[11]	Tripathi V, Parasuraman B, Laxmi A, et al. CIPK6, a CBL-interacting protein kinase is required for development and salt tolerance in plants[J]. The Plant Journal, 2009, 58(5): 778-790
[12]	Chen L, Ren F, Zhou L, et al. The Brassica napus Calcineurin B-Like 1/CBL-interacting protein kinase 6 (CBL1/CIPK6) component is involved in the plant response to abiotic stress and ABA signaling [J]. Journal of experimental botany, 2012, 63(17): 6211-6222
[13]	de la Torre F, Gutiérrez-Beltrán E, Pareja-Jaime Y, et al. The Tomato Calcium Sensor Cbl10 and Its Interacting Protein Kinase Cipk6 Define a Signaling Pathway in Plant Immunity [J]. The Plant Cell Online, 2013, 25(7):2748-2764
[14]	He L, Yang X, Wang L, et al. Molecular cloning and functional characterization of a novel cotton CBL-interacting protein kinase gene (< i>GhCIPK6) reveals its involvement in multiple abiotic stress tolerance in transgenic plants [J]. Biochemical and biophysical research communications, 2013
[15]	Wang R K, Li L L, Cao Z K, et al. Molecular cloning and functional characterization of a novel apple MdCIPK6L gene reveals its involvement in multiple abiotic stress tolerance in transgenic plants [J]. Plant Molecular Biology, 2012, 79(1-2): 123-135
[16]	Kiefer E, Heller W, Ernst D. A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites[J]. Plant Molecular Biology Reporter, 2000, 18(1): 33-39
[17]	Murray M, Thompson W F. Rapid isolation of high molecular weight plant DNA[J]. Nucleic acids research, 1980, 8(19): 4321-4326
[18]	王双双, 杨雪, 张伟. 钙调素类似蛋白参与拟南芥花粉萌发和花粉管生长的实验证据[J]. 中国科技论文在线, 2012
[19]	Hepler P K, Kunkel J G, Rounds C M, et al. Calcium entry into pollen tubes[J]. Trends in plant science 2012, 17(1):32-38
[20]	Konrad K R, Wudick M M, Feijó J A, et al. Calcium regulation of tip growth: new genes for old mechanisms[J]. Current opinion in plant biology 2011, 14(6):721-730
[21]	Michard E, Lima PT, Borges F, et al. Glutamate receptor-like genes form Ca²⁺ channels in pollen tubes and are regulated by pistil D-serine [J]. Science 2011, 332(6028):434-437
[22]	Steinhorst L, Kudla J. Calcium-a central regulator of pollen germination and tube growth [J]. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 2012
[23]	Yuasa T, Ishibashi Y, Lwaye-Inoue M, et al. A flower specific calcineurin B-like molecule (CBL)-interacting protein kinase (CIPK) homolog in tomato cultivar Micro-Tom (Solanum lycopersicum L.)[J]. American Journal of Plant Sciences, 2012, 3(6): 753-763.

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(2721) PDF downloads(1158) Cited by()

Proportional views

Molecular Cloning and Function Analysis of PtSIP3 Gene from Populus×euramericana cl.‘Zhonglin 46’Triploid

1. College of Life Sciences, Nankai University, Tianjin 300071, China

Received Date: 2013-08-22

Abstract: According to the preliminary results of microarray analysis, a high expression gene, PtSIP3, was cloned from Populus euramericana triploid, which generated from crosses between the maternal parent P. deltoides and the pollen parent P. nigra. The binary expression vector, pRI101- PtSIP3, was constructed and transformed into Arabidopsis. So far, the PtSIP3 transgenic plants were observed the remarkable phenotypic changes with inhibition of embryo and capsule development, improvement root development. Therefore, It is speculated that the rises of PtSIP3 expression may participate in the regulation of vegetative growth and promote root development in P. euramericana triploid.

HTML

Reference (23)

[1]	Stettler R, Zsuffa L, R.Wu. The role of hybridization in the genetic manipulation of Populus [J]. Biology of Populus and Its Implications for Management and Conservation (Part Ⅰ), 1996, 87-112
[2]	李善文, 张志毅, 何承忠. 中国杨树杂交育种研究进展[J]. 世界林业研究, 2004, 17(2): 37-41
[3]	Bradshaw Jr H, Stettler R. Stettler. Molecular genetics of growth and development in Populus. I. Triploidy in hybrid poplars[J]. Theoretical and Applied Genetics, 1993, 86(2-3): 301-307
[4]	Li Y, Kang X, Wang S, et al. Triploid induction in Populus alba x P. glandulosa by chromosome doubling of female gametes [J]. Silvae Genetica, 2008, 57(1): 37
[5]	Wu, R. Quantitative genetic variation of leaf size and shape in a mixed diploid and triploid population of Populus[J]. Genetical research, 2000, 75(2): 215-222
[6]	Sederoff, R. Regulatory science in forest biotechnology[J]. Tree Genetics & Genomes, 2007, 3(2): 71-74
[7]	Tuskan G A, Difazio S, Jansson S, et al. The genome of black cottonwood, Populus trichocarpa (Torr. & Gray) [J]. Science, 2006, 313(5793): 1596-1604
[8]	Tripathi V, Syed N, Laxmi A, et al. Role of CIPK6 in root growth and auxin transport [J]. Plant Signaling & Behavior, 2009, 4(7): 663-665
[9]	Kim M C, Chung W S, Yun D J, et al. Calcium and calmodulin-mediated regulation of gene expression in plants [J]. Molecular plant, 2009, 2(1): 13-21
[10]	Liu J, Zhu J K. A calcium sensor homolog required for plant salt tolerance [J]. Science, 1998, 280(5371): 1943-1945
[11]	Tripathi V, Parasuraman B, Laxmi A, et al. CIPK6, a CBL-interacting protein kinase is required for development and salt tolerance in plants[J]. The Plant Journal, 2009, 58(5): 778-790
[12]	Chen L, Ren F, Zhou L, et al. The Brassica napus Calcineurin B-Like 1/CBL-interacting protein kinase 6 (CBL1/CIPK6) component is involved in the plant response to abiotic stress and ABA signaling [J]. Journal of experimental botany, 2012, 63(17): 6211-6222
[13]	de la Torre F, Gutiérrez-Beltrán E, Pareja-Jaime Y, et al. The Tomato Calcium Sensor Cbl10 and Its Interacting Protein Kinase Cipk6 Define a Signaling Pathway in Plant Immunity [J]. The Plant Cell Online, 2013, 25(7):2748-2764
[14]	He L, Yang X, Wang L, et al. Molecular cloning and functional characterization of a novel cotton CBL-interacting protein kinase gene (< i>GhCIPK6) reveals its involvement in multiple abiotic stress tolerance in transgenic plants [J]. Biochemical and biophysical research communications, 2013
[15]	Wang R K, Li L L, Cao Z K, et al. Molecular cloning and functional characterization of a novel apple MdCIPK6L gene reveals its involvement in multiple abiotic stress tolerance in transgenic plants [J]. Plant Molecular Biology, 2012, 79(1-2): 123-135
[16]	Kiefer E, Heller W, Ernst D. A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites[J]. Plant Molecular Biology Reporter, 2000, 18(1): 33-39
[17]	Murray M, Thompson W F. Rapid isolation of high molecular weight plant DNA[J]. Nucleic acids research, 1980, 8(19): 4321-4326
[18]	王双双, 杨雪, 张伟. 钙调素类似蛋白参与拟南芥花粉萌发和花粉管生长的实验证据[J]. 中国科技论文在线, 2012
[19]	Hepler P K, Kunkel J G, Rounds C M, et al. Calcium entry into pollen tubes[J]. Trends in plant science 2012, 17(1):32-38
[20]	Konrad K R, Wudick M M, Feijó J A, et al. Calcium regulation of tip growth: new genes for old mechanisms[J]. Current opinion in plant biology 2011, 14(6):721-730
[21]	Michard E, Lima PT, Borges F, et al. Glutamate receptor-like genes form Ca²⁺ channels in pollen tubes and are regulated by pistil D-serine [J]. Science 2011, 332(6028):434-437
[22]	Steinhorst L, Kudla J. Calcium-a central regulator of pollen germination and tube growth [J]. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 2012
[23]	Yuasa T, Ishibashi Y, Lwaye-Inoue M, et al. A flower specific calcineurin B-like molecule (CBL)-interacting protein kinase (CIPK) homolog in tomato cultivar Micro-Tom (Solanum lycopersicum L.)[J]. American Journal of Plant Sciences, 2012, 3(6): 753-763.

Molecular Cloning and Function Analysis of PtSIP3 Gene from Populus×euramericana cl.‘Zhonglin 46’Triploid

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

Molecular Cloning and Function Analysis of PtSIP3 Gene from Populus×euramericana cl.‘Zhonglin 46’Triploid

HTML

Catalog

Export File

Citation

Format

Content