Expression Analysis of 5 miRNAs and Their Targets During Different Growth and Developmental Stages of Caragana intermedia

ZHU Jian-feng; LI Wan-feng; YANG Wen-hua; HAN Su-ying; QI Li-wang

Volume 26 Issue S1

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Expression Analysis of 5 miRNAs and Their Targets During Different Growth and Developmental Stages of Caragana intermedia

1.
Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
2.
Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China

Received Date: 2013-08-24

Abstract

The expression patterns of 5 miRNAs(cin-miR157a, cin-miR159a, cin-miR165a, cin-miR172b and cin-miR396a) and their targets during different organs in different developmental stages were assessed by qRT-PCR. The results showed that the expression patterns of all the miRNAs are of temporal and spatial specificity. 4 miRNAs(cin-miR159a, cin-miR165a, cin-miR172b and cin-miR396a) are highly expressed in vegetative organs of mature period; one miRNA (cin-miR157a) is highly expressed in juvenile stage and reproduction organs of mature period. The results also showed that three miRNAs (cin-miR157a, cin-miR172b and cin-miR396a) and their target genes were related to the growth and decline in different developmental stages and different organs. High expression of miRNA and its target gene expression are often low. When the miRNA expression is low, its target gene expression is often high. This result indicates that miRNA may play an important role during the growth and development of C. intermedia.
- Caragana intermedia,
- growth and development,
- microRNA,
- target genes,
- expression analysis

References

[1]	Ambros V. MicroRNAs: tiny regulators with great potential[J]. Cell, 2001, 107: 823-826
[2]	Bartel D P. MicroRNAs: genomics, biogenesis, mechanism, and function[J]. Cell, 2004, 116: 281-297
[3]	Carrington J C, Ambros V. Role of microRNAs in plant and animal development[J]. Science, 2003, 301: 336-338
[4]	Lee R C, Feinbaum R L, Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14[J]. Cell, 1993, 75: 843-854
[5]	Lagos-Quintana M, Rauhut R, Lendeckel W, et al. Identification of novel genes coding for small expressed RNAs[J]. Science, 2001, 294: 853-858
[6]	Lau N C, Lim L P, Weinstein E G, et al. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans[J]. Science, 2001, 294: 858-862
[7]	Lee R C, Ambros V. An extensive class of small RNAs in Caenorhabditis elegans[J]. Science, 2001, 294: 862-864
[8]	Llave C, Kasschau K D, Rector M A, et al. Endogenous and silencing-associated small RNAs in plants[J]. Plant Cell, 2002, 14: 1605-1619
[9]	Reinhart B J, Weinstein E G, Rhoades M W, et al. MicroRNAs in plants[J]. Genes Dev, 2002, 16: 1616-1626
[10]	Kurihara Y, Watanabe Y. Arabidopsis micro-RNA biogenesis through Dicer-like 1 protein functions[J]. Proc Natl Acad Sci USA, 2004, 101: 12753-12758
[11]	Qi Y, Denli A M, Hannon G J. Biochemical specialization within Arabidopsis RNA silencing pathways[J]. Molecular Cell, 2005, 19: 421-428
[12]	Jones-Rhoades M W, Bartel D P, Bartel B. MicroRNAs and their regulatory roles in plants[J]. Annu Rev Plant Biol, 2006, 57: 19-53.
[13]	Chiou T J, Aung K, Lin S I, et al. Regulation of phosphate homeostasis by MicroRNA in Arabidopsis[J]. Plant Cell, 2006, 18: 412-421
[14]	Zhang B, Pan X, Cobb G P, et al. Plant microRNA: a small regulatory molecule with big impact [J]. Developmental Biology, 2006, 289: 3-16
[15]	Jia S S, Zhou G K. Isolation and identification of isoflavones in the root of Caragana intermedia Kuang[J]. Zhong Yao Tong Bao, 1988, 13: 34-35, 59
[16]	牛西午. 中国锦鸡儿属植物资源研究—分布及分种描述[J]. 西北植物学报, 1999, 19 (5): 107-133
[17]	牛西午. 柠条研究[M]. 北京:科学出版社, 2003: 1-2
[18]	Shi S Q, Shi Z, Jiang Z P, et al. Effects of exogenous GABA on gene expression of Caragana intermedia roots under NaCl stress: regulatory roles for H₂O₂ and ethylene production [J]. Plant Cell and Environment,2010, 33: 149-162
[19]	Xu Z Z, Zhou G S, Wang Y H. Combined effects of elevated CO₂ and soil drought on carbon and nitrogen allocation of the desert shrub Caragana intermedia[J]. Plant and Soil, 2007, 301: 87-97
[20]	Guo W H, Li B, Zhang X S, et al. Effects of water stress on water use efficiency and water balance components of Hippophae rhamnoides and Caragana intermedia in the soil-plant-atmosphere continuum [J]. Agroforestry Systems, 2010, 80: 423-435
[21]	De Paola D, Cattonaro F, Pignone D, et al. The miRNAome of globe artichoke: conserved and novel micro RNAs and target analysis[J]. Bmc Genomics, 2012, 13: 41
[22]	Xie F, Frazier T P, Zhang B. Identification and characterization of microRNAs and their targets in the bioenergy plant switchgrass (Panicum virgatum)[J]. Planta, 2010, 232: 417-434
[23]	Willmann M R, Poethig R S. Time to grow up: the temporal role of smallRNAs in plants[J]. Curr Opin Plant Biol, 2005, 8: 548-552
[24]	Wang J W, Czech B, Weigel D. miR156-regulated SPL transcription factors define an endogenous flowering pathway in Arabidopsis thaliana[J]. Cell, 2009, 138: 738-749
[25]	Wu G, Park M Y, Conway S R, et al. The sequential action of miR156 and miR172 regulates developmental timing in Arabidopsis[J]. Cell, 2009, 138: 750-759
[26]	Chuck G, Meeley R, Hake S. Floral meristem initiation and meristem cell fate are regulated by the maize AP2 genes ids1 and sid1 [J]. Development, 2008, 135: 3013-3019
[27]	Rodriguez R E, Mecchia M A, Debernardi J M, et al. Control of cell proliferation in Arabidopsis thaliana by microRNA miR396[J]. Development, 2010, 137: 103-112

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Expression Analysis of 5 miRNAs and Their Targets During Different Growth and Developmental Stages of Caragana intermedia

1. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

2. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China

Received Date: 2013-08-24

Abstract: The expression patterns of 5 miRNAs(cin-miR157a, cin-miR159a, cin-miR165a, cin-miR172b and cin-miR396a) and their targets during different organs in different developmental stages were assessed by qRT-PCR. The results showed that the expression patterns of all the miRNAs are of temporal and spatial specificity. 4 miRNAs(cin-miR159a, cin-miR165a, cin-miR172b and cin-miR396a) are highly expressed in vegetative organs of mature period; one miRNA (cin-miR157a) is highly expressed in juvenile stage and reproduction organs of mature period. The results also showed that three miRNAs (cin-miR157a, cin-miR172b and cin-miR396a) and their target genes were related to the growth and decline in different developmental stages and different organs. High expression of miRNA and its target gene expression are often low. When the miRNA expression is low, its target gene expression is often high. This result indicates that miRNA may play an important role during the growth and development of C. intermedia.

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Reference (27)

[1]	Ambros V. MicroRNAs: tiny regulators with great potential[J]. Cell, 2001, 107: 823-826
[2]	Bartel D P. MicroRNAs: genomics, biogenesis, mechanism, and function[J]. Cell, 2004, 116: 281-297
[3]	Carrington J C, Ambros V. Role of microRNAs in plant and animal development[J]. Science, 2003, 301: 336-338
[4]	Lee R C, Feinbaum R L, Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14[J]. Cell, 1993, 75: 843-854
[5]	Lagos-Quintana M, Rauhut R, Lendeckel W, et al. Identification of novel genes coding for small expressed RNAs[J]. Science, 2001, 294: 853-858
[6]	Lau N C, Lim L P, Weinstein E G, et al. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans[J]. Science, 2001, 294: 858-862
[7]	Lee R C, Ambros V. An extensive class of small RNAs in Caenorhabditis elegans[J]. Science, 2001, 294: 862-864
[8]	Llave C, Kasschau K D, Rector M A, et al. Endogenous and silencing-associated small RNAs in plants[J]. Plant Cell, 2002, 14: 1605-1619
[9]	Reinhart B J, Weinstein E G, Rhoades M W, et al. MicroRNAs in plants[J]. Genes Dev, 2002, 16: 1616-1626
[10]	Kurihara Y, Watanabe Y. Arabidopsis micro-RNA biogenesis through Dicer-like 1 protein functions[J]. Proc Natl Acad Sci USA, 2004, 101: 12753-12758
[11]	Qi Y, Denli A M, Hannon G J. Biochemical specialization within Arabidopsis RNA silencing pathways[J]. Molecular Cell, 2005, 19: 421-428
[12]	Jones-Rhoades M W, Bartel D P, Bartel B. MicroRNAs and their regulatory roles in plants[J]. Annu Rev Plant Biol, 2006, 57: 19-53.
[13]	Chiou T J, Aung K, Lin S I, et al. Regulation of phosphate homeostasis by MicroRNA in Arabidopsis[J]. Plant Cell, 2006, 18: 412-421
[14]	Zhang B, Pan X, Cobb G P, et al. Plant microRNA: a small regulatory molecule with big impact [J]. Developmental Biology, 2006, 289: 3-16
[15]	Jia S S, Zhou G K. Isolation and identification of isoflavones in the root of Caragana intermedia Kuang[J]. Zhong Yao Tong Bao, 1988, 13: 34-35, 59
[16]	牛西午. 中国锦鸡儿属植物资源研究—分布及分种描述[J]. 西北植物学报, 1999, 19 (5): 107-133
[17]	牛西午. 柠条研究[M]. 北京:科学出版社, 2003: 1-2
[18]	Shi S Q, Shi Z, Jiang Z P, et al. Effects of exogenous GABA on gene expression of Caragana intermedia roots under NaCl stress: regulatory roles for H₂O₂ and ethylene production [J]. Plant Cell and Environment,2010, 33: 149-162
[19]	Xu Z Z, Zhou G S, Wang Y H. Combined effects of elevated CO₂ and soil drought on carbon and nitrogen allocation of the desert shrub Caragana intermedia[J]. Plant and Soil, 2007, 301: 87-97
[20]	Guo W H, Li B, Zhang X S, et al. Effects of water stress on water use efficiency and water balance components of Hippophae rhamnoides and Caragana intermedia in the soil-plant-atmosphere continuum [J]. Agroforestry Systems, 2010, 80: 423-435
[21]	De Paola D, Cattonaro F, Pignone D, et al. The miRNAome of globe artichoke: conserved and novel micro RNAs and target analysis[J]. Bmc Genomics, 2012, 13: 41
[22]	Xie F, Frazier T P, Zhang B. Identification and characterization of microRNAs and their targets in the bioenergy plant switchgrass (Panicum virgatum)[J]. Planta, 2010, 232: 417-434
[23]	Willmann M R, Poethig R S. Time to grow up: the temporal role of smallRNAs in plants[J]. Curr Opin Plant Biol, 2005, 8: 548-552
[24]	Wang J W, Czech B, Weigel D. miR156-regulated SPL transcription factors define an endogenous flowering pathway in Arabidopsis thaliana[J]. Cell, 2009, 138: 738-749
[25]	Wu G, Park M Y, Conway S R, et al. The sequential action of miR156 and miR172 regulates developmental timing in Arabidopsis[J]. Cell, 2009, 138: 750-759
[26]	Chuck G, Meeley R, Hake S. Floral meristem initiation and meristem cell fate are regulated by the maize AP2 genes ids1 and sid1 [J]. Development, 2008, 135: 3013-3019
[27]	Rodriguez R E, Mecchia M A, Debernardi J M, et al. Control of cell proliferation in Arabidopsis thaliana by microRNA miR396[J]. Development, 2010, 137: 103-112

Expression Analysis of 5 miRNAs and Their Targets During Different Growth and Developmental Stages of Caragana intermedia

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通讯作者: 陈斌, bchen63@163.com

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Expression Analysis of 5 miRNAs and Their Targets During Different Growth and Developmental Stages of Caragana intermedia

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