• 中国中文核心期刊
  • 中国科学引文数据库(CSCD)核心库来源期刊
  • 中国科技论文统计源期刊(CJCR)
  • 第二届国家期刊奖提名奖

Citation:

Isolation and Expression Analysis of DNA Methyltransferase Fragments in Populus×euramericana

  • Received Date: 2013-08-20
  • Fragments of eight specific DNA methyltransferases belonging to MET, CMT, DRM and DNMT2 were isolated from triploid Populus euramericana by using homology cloning techniques. The homology analysis indicated that five out of eight DNA methyltransferases showed 100% homology with the corresponding sequences of diploid, and splicing changes in other three genes were detected. Real-time quantitative PCR analysis detected the difference in transcript expression of these eight DNA methyltransferases in different materials, different tissues and different growth phases. Further analysis indicated that the DNA methylation status in the same tissues of different ploidy poplars was involved in different expression of different DNA methylation. Based on the results of analyzing the transcript expression of these DNA methyltransferases in different growth phases, it suggested that the members of MET gene family (PnD1 and PnD2) and CMT gene family (PnD3 and PnD4) may participate in antagonistic regulation and coordinate regulation, respectively. In addition, the results indicated that the PnD6 which belongs to DNMT2 gene family was weakly expressed in different tissues except in the stem tip of triploid P. euramericana. It implied that PnD6 may play an important role in the fast-growing of triploid P. euramericana. All these results suggested that DNA methyltransferases gene may be involved in the entire process of leaf morphogenesis.
  • 加载中
  • [1]

    Tariq M, Saze H, Probst A V, et al. Erasure of CpG methylation in Arabidopsis alters patterns of histone H3 methylation in heterochromatin[J]. Proc Natl Acad Sci USA, 2003, 100(15): 8823-8827
    [2]

    Bartee L, Bender J. Two Arabidopsis methylation-deficiency mutations confer only partial effects on a methylated endogenous gene family[J]. Nucleic Acids Res, 2001, 29(10): 2127-2134
    [3]

    Lindroth, A M, Cao X, Jackson J P, et al. Requirement of CHROMOMETHYLASE3 for maintenance of CpXpG methylation[J]. Science, 2001, 292(5524):2077-2080
    [4]

    Cao X, Springer N M, Muszynski M G, et al. Conserved plant genes with similarity to mammalian de novo DNA methyltransferases[J]. Proc Natl Acad Sci USA, 2000, 97(9): 4979-4984
    [5]

    Mueller T D, Feigon, J. Solution structures of UBA domains reveal a conserved hydrophobic surface for protein-protein interactions[J]. J Mol Biol, 2002, 319(5): 1243-1255
    [6]

    Goll M G, Kirpekar F, Maggert K A, et al. Methylation of tRNAAsp by the DNA methyltransferase homolog Dnmt2[J]. Science, 2006, 311(5759): 395-398
    [7]

    Hu B, Han C, Wang B, Song W, et al. Cytosine methylation at CG and CNG sites is differential during the development of triploid black poplar[J]. J Plant Biochem Biotechnol, 2012, DOI:10.1007/s13562-012-0170-x
    [8]

    Kiefer E, Heller W, Ernst D. A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites[J]. Plant Mol Biol Rep, 2000, 18(1): 33-39
    [9]

    Chan S W L, Henderson I R, Jacobsen S E. Gardening the genome: DNA methylation in Arabidopsis thaliana[J]. Nat Rev Genet, 2005, 6(5): 351-360
    [10]

    Yamauchi T, Moritoh S, Johzuka-Hisatomi Y, et al. Alternative splicing of the rice OsMET1 genes encoding maintenance DNA methyltransferase[J]. Journal of plant physiology, 2008, 165(17):1774-1782
    [11]

    Teerawanichpan P, Krittanai P, Chauvatcharin N, et al. Purification and characterization of rice DNA methyltransferase[J]. Plant physiology and biochemistry, 2009, 47(8):671-680
    [12]

    Zhang X, Yazaki J, Sundaresan A, et al. Genome-wide high-resolution mapping and functional analysis of DNA methylation in arabidopsis[J]. Cell, 2006, 126(6):1189-1201
    [13]

    Suzuki M M, Bird A. DNA methylation landscapes: provocative insights from epigenomics[J]. Nat Rev Genet, 2008, 9(6):465-476
    [14]

    Xiao W, Custard K D, Brown R C, et al. DNA methylation is critical for Arabidopsis embryogenesis and seed viability[J]. The Plant Cell Online, 2006, 18(4):805-814
    [15]

    Cokus S J, Feng S, Zhang X, et al. Shotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterning[J]. Nature, 2008, 452(7184):215-219
    [16]

    Chan S W, Henderson I R, Jacobsen S E. Gardening the genome: DNA methylation in Arabidopsis thaliana[J]. Nat Rev Genet, 2005, 6(5):351-360
    [17]

    Cao X, Jacobsen S E. Locus-specific control of asymmetric and CpNpG methylation by the DRM and CMT3 methyltransferase genes[J]. Proc Natl Acad Sci U S A, 2002, 99(Suppl 4): 16491-16498
    [18]

    Cao X, Jacobsen S E. Role of the Arabidopsis DRM methyltransferases in de novo DNA methylation and gene silencing[J]. Curr Biol, 2002, 12(13): 1138-1144
    [19]

    Rangani G, Khodakovskaya M, Alimohammadi M, et al. Site-specific methylation in gene coding region underlies transcriptional silencing of the Phytochrome A epiallele in Arabidopsis thaliana[J]. Plant molecular biology, 2012, 79: 191-202
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article views(2752) PDF downloads(1271) Cited by()

Proportional views

Isolation and Expression Analysis of DNA Methyltransferase Fragments in Populus×euramericana

  • 1. College of Life Sciences, Nankai University, Tianjin 300071, China

Abstract: Fragments of eight specific DNA methyltransferases belonging to MET, CMT, DRM and DNMT2 were isolated from triploid Populus euramericana by using homology cloning techniques. The homology analysis indicated that five out of eight DNA methyltransferases showed 100% homology with the corresponding sequences of diploid, and splicing changes in other three genes were detected. Real-time quantitative PCR analysis detected the difference in transcript expression of these eight DNA methyltransferases in different materials, different tissues and different growth phases. Further analysis indicated that the DNA methylation status in the same tissues of different ploidy poplars was involved in different expression of different DNA methylation. Based on the results of analyzing the transcript expression of these DNA methyltransferases in different growth phases, it suggested that the members of MET gene family (PnD1 and PnD2) and CMT gene family (PnD3 and PnD4) may participate in antagonistic regulation and coordinate regulation, respectively. In addition, the results indicated that the PnD6 which belongs to DNMT2 gene family was weakly expressed in different tissues except in the stem tip of triploid P. euramericana. It implied that PnD6 may play an important role in the fast-growing of triploid P. euramericana. All these results suggested that DNA methyltransferases gene may be involved in the entire process of leaf morphogenesis.

Reference (19)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return