Decolorization and Antioxidant Assay of Polysaccharide from White Wax Scale Using Macroporous Resin

HE Zhao; LI Xian; CHEN Zhi-yong; SUN Long; FENG Ying

Volume 27 Issue 1

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Decolorization and Antioxidant Assay of Polysaccharide from White Wax Scale Using Macroporous Resin

1.
Research Institute of Resources Insects, Chinese Academy of Forestry
2.
Laboratory of Cultivation and Utilization of Resources Insects, State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2013-09-20

Abstract

The decoloring effect of 12 macroporous resins on crude polysaccharide extracted from white wax scale, Ericeruspela pela (Chavannes), was studied. The cellular antioxidant activity of crude and decolorant polysaccharide on proliferation effect of SH-SY5Y cells induced by hydrogen peroxide were evaluated. The resin NKA-9 was selected for kinetic study based on the results of adsorption properties comparing of macroporous adsorption resins and the static adsorption test. The dynamic adsorption and desorption experiments were conducted to establish the optimum parameters of NKA-9 as: one volume solution of crude polysaccharide was loaded in column at the flow rate 1 bed volume per hour under the condition of 30 ℃ and pH 8.0. The decolorant polysaccharide was collected by washing adsorbate-laden column using five bed volume of deionized water. The decoloration rate was 71.4%, the yield of polysaccharide was 60%, the deproteinization rate was 51.9%. The results of cellular antioxidant activity assay showed that the cell livability could be increased only at the concentration of 1 000 μg·mL-1 crude polysaccharide. But the decolorant polysaccharide at the concentrations of 10 1 000 μg·mL-1 could reduce the cell death rate of SH-SY5Y. Among them, the decolorant polysaccharide at the concentrations of 1 000μg·mL-1 could reduce the cell death rate notably. The cellular antioxidant activity of decolorant polysaccharide was enhanced after decoloring by NKA-9.
- white wax scale,
- polysaccharide,
- decoloring,
- macroporous resin,
- antioxidant,
- human neuroblastoma cell line (SH-SY5Y)

References

[1]	陈晓鸣, 冯颖. 资源昆虫学概论[M]. 北京:科学出版社, 2009:29-30
[2]	冯颖, 陈晓鸣, 马艳, 等. 白蜡虫免疫调节作用试验研究[J]. 林业科学研究, 2006, 19(2):221-224
[3]	冯颖, 陈晓鸣, 何钊, 等. 白蜡虫抗突变实验与主要功效成分分析[J]. 林业科学研究, 2006, 19(3):284-288
[4]	何钊, 冯颖, 孙龙, 等. 黄粉虫多糖响应面法提取及抗氧化活性[J]. 食品与生物技术学报, 2011, 30(5):641-647
[5]	孙龙, 冯颖, 何钊, 等. 蟑螂水溶性多糖提取、分析及免疫活性研究[J]. 林业科学研究, 2009, 22(2):256-261
[6]	孙龙, 冯颖, 何钊, 等. 蚕蛹多糖的碱液提取及免疫活性初步研究[J]. 林业科学研究, 2007, 20(6):782-786
[7]	何钊, 孙龙, 冯颖, 等. 白蜡虫多糖的提取及单糖组分分析[J]. 林业科学研究, 2008, 21(6):792-796
[8]	杨云, 田润涛, 苗明三, 等. 大枣渣多糖活性炭脱色工艺研究[J]. 河南中医学院学报, 2004, 19(1):35-36
[9]	Jian-Hua Xie, Ming-Yue Shen, Shao-Ping Nie, et al. Decolorization of polysaccharides solution from cyclocarya paliurus (batal.) Iljinskaja using ultrasound/h2o2 process[J]. Carbohydrate Polymers, 2011, 84(1):255-261
[10]	卢国勇, 孟江, 廖华卫. 鱼腥草多糖双氧水脱色工艺研究[J]. 时珍国医国药, 2011, 22(3):671-673
[11]	李文芳, 向昌国, 罗庆华, 等. 南瓜藤蔓中多糖脱色工艺的研究及其含量变化[J]. 食品科学, 2006, 27(12):442-445
[12]	宿玮, 王彦超, 张翠玉, 等. 海地瓜多糖脱色工艺研究[J]. 离子交换与吸附, 2011, 27(6):521-529
[13]	王丽波, 赵玉, 徐雅琴. 南瓜多糖大孔吸附树脂纯化工艺[J]. 农业机械学报, 2010(9):138-142
[14]	Liu J, Luo J, Sun Y, et al. A simple method for the simultaneous decoloration and deproteinization of crude levan extract from paenibacillus polymyxa ejs-3 by macroporous resin[J]. Bioresour Technol, 2010, 101(15):6077-6083
[15]	王松柏, 秦雪梅, 郭小青, 等. 树脂对防风粗多糖脱色效果[J]. 应用化学, 2005, 22(12):1308-1311
[16]	杨云, 冯卫生, 雷高明. 大枣渣多糖精制纯化工艺的研究[J]. 中药材, 2006, 29(1):78-80
[17]	付学鹏, 杨晓杰. 植物多糖脱色技术的研究[J]. 食品研究与开发, 2007, 28(11):166-169
[18]	肖丽霞, 于洪涛, 胡晓松. 香菇多糖的树脂脱色工艺研究[J]. 食品与机械, 2011, 27(6):241-244
[19]	谢红旗, 周春山. 香菇多糖脱色工艺研究[J]. 离子交换与吸附, 2007, 23(2):158-165
[20]	王元凤, 金征宇. 茶多糖脱色的研究[J]. 食品与发酵工业, 2004, 30(12):60-65
[21]	Cho E S, Lee K W, Lee H J. Cocoa procyanidins protect pc12 cells from hydrogen-peroxide-induced apoptosis by inhibiting activation of p38 mapk and jnk[J]. Mutat Res, 2008, 640(1-2):123-130
[22]	Xiu-Lian Chang, Dong Wang, Bi-Yun Chen, et al. Adsorption and desorption properties of macroporous resins for anthocyanins from the calyx extract of roselle (Hibiscus sabdariffa L.)[J]. Journal of Agricultural and Food Chemistry, 2012, 60(9):2368-2376

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Decolorization and Antioxidant Assay of Polysaccharide from White Wax Scale Using Macroporous Resin

1. Research Institute of Resources Insects, Chinese Academy of Forestry

2. Laboratory of Cultivation and Utilization of Resources Insects, State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2013-09-20

Abstract: The decoloring effect of 12 macroporous resins on crude polysaccharide extracted from white wax scale, Ericeruspela pela (Chavannes), was studied. The cellular antioxidant activity of crude and decolorant polysaccharide on proliferation effect of SH-SY5Y cells induced by hydrogen peroxide were evaluated. The resin NKA-9 was selected for kinetic study based on the results of adsorption properties comparing of macroporous adsorption resins and the static adsorption test. The dynamic adsorption and desorption experiments were conducted to establish the optimum parameters of NKA-9 as: one volume solution of crude polysaccharide was loaded in column at the flow rate 1 bed volume per hour under the condition of 30 ℃ and pH 8.0. The decolorant polysaccharide was collected by washing adsorbate-laden column using five bed volume of deionized water. The decoloration rate was 71.4%, the yield of polysaccharide was 60%, the deproteinization rate was 51.9%. The results of cellular antioxidant activity assay showed that the cell livability could be increased only at the concentration of 1 000 μg·mL-1 crude polysaccharide. But the decolorant polysaccharide at the concentrations of 10 1 000 μg·mL-1 could reduce the cell death rate of SH-SY5Y. Among them, the decolorant polysaccharide at the concentrations of 1 000μg·mL-1 could reduce the cell death rate notably. The cellular antioxidant activity of decolorant polysaccharide was enhanced after decoloring by NKA-9.

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Reference (22)

[1]	陈晓鸣, 冯颖. 资源昆虫学概论[M]. 北京:科学出版社, 2009:29-30
[2]	冯颖, 陈晓鸣, 马艳, 等. 白蜡虫免疫调节作用试验研究[J]. 林业科学研究, 2006, 19(2):221-224
[3]	冯颖, 陈晓鸣, 何钊, 等. 白蜡虫抗突变实验与主要功效成分分析[J]. 林业科学研究, 2006, 19(3):284-288
[4]	何钊, 冯颖, 孙龙, 等. 黄粉虫多糖响应面法提取及抗氧化活性[J]. 食品与生物技术学报, 2011, 30(5):641-647
[5]	孙龙, 冯颖, 何钊, 等. 蟑螂水溶性多糖提取、分析及免疫活性研究[J]. 林业科学研究, 2009, 22(2):256-261
[6]	孙龙, 冯颖, 何钊, 等. 蚕蛹多糖的碱液提取及免疫活性初步研究[J]. 林业科学研究, 2007, 20(6):782-786
[7]	何钊, 孙龙, 冯颖, 等. 白蜡虫多糖的提取及单糖组分分析[J]. 林业科学研究, 2008, 21(6):792-796
[8]	杨云, 田润涛, 苗明三, 等. 大枣渣多糖活性炭脱色工艺研究[J]. 河南中医学院学报, 2004, 19(1):35-36
[9]	Jian-Hua Xie, Ming-Yue Shen, Shao-Ping Nie, et al. Decolorization of polysaccharides solution from cyclocarya paliurus (batal.) Iljinskaja using ultrasound/h2o2 process[J]. Carbohydrate Polymers, 2011, 84(1):255-261
[10]	卢国勇, 孟江, 廖华卫. 鱼腥草多糖双氧水脱色工艺研究[J]. 时珍国医国药, 2011, 22(3):671-673
[11]	李文芳, 向昌国, 罗庆华, 等. 南瓜藤蔓中多糖脱色工艺的研究及其含量变化[J]. 食品科学, 2006, 27(12):442-445
[12]	宿玮, 王彦超, 张翠玉, 等. 海地瓜多糖脱色工艺研究[J]. 离子交换与吸附, 2011, 27(6):521-529
[13]	王丽波, 赵玉, 徐雅琴. 南瓜多糖大孔吸附树脂纯化工艺[J]. 农业机械学报, 2010(9):138-142
[14]	Liu J, Luo J, Sun Y, et al. A simple method for the simultaneous decoloration and deproteinization of crude levan extract from paenibacillus polymyxa ejs-3 by macroporous resin[J]. Bioresour Technol, 2010, 101(15):6077-6083
[15]	王松柏, 秦雪梅, 郭小青, 等. 树脂对防风粗多糖脱色效果[J]. 应用化学, 2005, 22(12):1308-1311
[16]	杨云, 冯卫生, 雷高明. 大枣渣多糖精制纯化工艺的研究[J]. 中药材, 2006, 29(1):78-80
[17]	付学鹏, 杨晓杰. 植物多糖脱色技术的研究[J]. 食品研究与开发, 2007, 28(11):166-169
[18]	肖丽霞, 于洪涛, 胡晓松. 香菇多糖的树脂脱色工艺研究[J]. 食品与机械, 2011, 27(6):241-244
[19]	谢红旗, 周春山. 香菇多糖脱色工艺研究[J]. 离子交换与吸附, 2007, 23(2):158-165
[20]	王元凤, 金征宇. 茶多糖脱色的研究[J]. 食品与发酵工业, 2004, 30(12):60-65
[21]	Cho E S, Lee K W, Lee H J. Cocoa procyanidins protect pc12 cells from hydrogen-peroxide-induced apoptosis by inhibiting activation of p38 mapk and jnk[J]. Mutat Res, 2008, 640(1-2):123-130
[22]	Xiu-Lian Chang, Dong Wang, Bi-Yun Chen, et al. Adsorption and desorption properties of macroporous resins for anthocyanins from the calyx extract of roselle (Hibiscus sabdariffa L.)[J]. Journal of Agricultural and Food Chemistry, 2012, 60(9):2368-2376

Decolorization and Antioxidant Assay of Polysaccharide from White Wax Scale Using Macroporous Resin

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Decolorization and Antioxidant Assay of Polysaccharide from White Wax Scale Using Macroporous Resin

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