Virulence Bioassay of Different Metarhizium Strains Against Melanotus cribricollis Larvae

ZHANG Ya-bo; YE Bi-huan; LIU Zhen-yong; ZHANG Wei; SHU Jin-ping

The bamboo wireworm Melanotus cribricollis is a devastating pest of underground bamboo shoots in southern China. To explore an alternative control strategy with fungal biocontrol agents, the authors bioassayed the virulence of three Metarhizium strains against Melanotus cribricollis larvae (120-150 mg per capita) in small cups filled with 50 g soil containing 5×106 conidia per gram of soil. The application of the stains Metarhizium pingshaense WP08 and Metarhizium anisopliae 30104 resulted in the corrected mortalities of 90.0% and 66.3% during a 30-day period under controlled conditions, respectively, whereas Metarhizium anisopliae LRC112 showed no lethal effect on the larvae. Time-mortality analysis led to the median lethal time (LT50) estimates of 15.8 and 25.8 days for the two virulent strains. The high-virulence strain Metarhizium pingshaense WP08 was further assayed for its lethal effect on the larvae in contact of conidia mixed with corn bait and soil respectively. As a result, the soil mixture caused higher mortality (60.1%) and shorter LT50 (22.6 days) than the bait mixture (52.0%; 28.0 days).

1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China

2. Forest Pest Management and Quarantine Station of Deqing County, Zhejiang Provinc, Deqing 313299, Zhejiang, China

3. Forest Pest Management and Quarantine Station of Tongchuan City, Shaanxi Province, Tongchuan 727007, Shaanxi, China

Received Date: 2013-02-22

Abstract: The bamboo wireworm Melanotus cribricollis is a devastating pest of underground bamboo shoots in southern China. To explore an alternative control strategy with fungal biocontrol agents, the authors bioassayed the virulence of three Metarhizium strains against Melanotus cribricollis larvae (120-150 mg per capita) in small cups filled with 50 g soil containing 5×106 conidia per gram of soil. The application of the stains Metarhizium pingshaense WP08 and Metarhizium anisopliae 30104 resulted in the corrected mortalities of 90.0% and 66.3% during a 30-day period under controlled conditions, respectively, whereas Metarhizium anisopliae LRC112 showed no lethal effect on the larvae. Time-mortality analysis led to the median lethal time (LT50) estimates of 15.8 and 25.8 days for the two virulent strains. The high-virulence strain Metarhizium pingshaense WP08 was further assayed for its lethal effect on the larvae in contact of conidia mixed with corn bait and soil respectively. As a result, the soil mixture caused higher mortality (60.1%) and shorter LT50 (22.6 days) than the bait mixture (52.0%; 28.0 days).

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[1]	徐天森, 王浩杰. 中国竹子主要害虫[M]. 北京: 中国林业出版社, 2004:26-27, 45-50
[2]	Chalfant R B, Jansson R K, Seal D R, et al. Ecology and management of sweet potato insects[J].Annual Review of Entomology, 1990, 35(1):157-180
[3]	Parker W E, Howard J J. The biology and management of wireworms (Agriotes spp.) on potato with particular reference to U.K.[J].Agricultural and Forest Entomology, 2001, 3(2):85-98
[4]	Shamiyeh N B, Pereiira R, Straw R A, et al. Control of wireworms in potato[J].Arthropod Management ests, 1999, 24:164-165
[5]	Roberts D W, St Leger R J. Metarhizium spp., osmopolitan insect-pathogenic fungi:mycological aspects[J]. Advances in Applied Microbiology, 2004, 54:1-70
[6]	詹儒林, 覃伟权, 宋妍, 等. 海南椰心叶甲病原菌金龟子绿僵菌的分离、鉴定及其生防潜力[J].生态学报, 2007, 27(4):1558-1562
[7]	何学友, 蔡守平, 余培旺, 等. 金龟子绿僵菌MaY'I'TR.04菌株对松墨天牛成虫的致病力[J].昆虫学报, 2008, 51(1):102-107
[8]	Fox J S, Jaques R P. Note on the green-muscardine fungus, Metarrhuzium anisopliae (Metch.) Sor., as a control for wireworms[J]. The Canadian Entomologist, 1958, 15(5): 314-315
[9]	Filipchuk O D, Yaroshenko V A, Ismailov V Y, et al. Effectiveness of biological and chemical preparations against tobacco pests[J]. Agrokhimiya, 1995, 9(8): 81-86
[10]	Kabaluk J T, Goettel M, Erlandson M, et al. Metarhizium anisopliae as a biological control for wireworms and a report of some other naturally-occurring parasites[J]. IOBC/WPRS Bulletin, 2005, 28(2): 109-115
[11]	王鹏, 张亚波, 舒金平, 等. 金龟子绿僵菌小孢变种对筛胸梳爪叩甲幼虫致病力的生物测定[J].中国生物防治, 2010, 26(3):274-279
[12]	问锦曾, 雷仲仁, 谭正华, 等. 5株绿僵菌对东亚飞蝗的毒力测定[J].植物保护, 2003, 29(3):50-52
[13]	单乐天, 冯明光. 不同寄主及地理来源的16株绿僵菌对桃蚜的毒力比较[J].微生物学报, 2006, 46(4):602-607
[14]	Kabaluk J T, Ericsson J D. Metarhizium anisopliae seed treatment increases yield of yield corn when applied for wireworm control[J]. Agronomy Journal, 2007, 99(5):1377-1381
[15]	Ericsson, J D, KabalukJ T, Goettel M S, et al. Spinosad interacts synergistically with the insect pathogen Metarhizium anisopliae against two exotic wireworms, Agriotes lineatus and A. obscurus (Coleoptera: Elateridae)[J].J Econ Entomol, 2007, 100(1):31-38
[16]	黄勃, 樊美珍, 李增智. 绿僵菌属系统分类的研究进展(综述)[J].安徽农业大学学报, 2002, 29(2):169-172
[17]	Ansari M A, Evans M, Butt T M. Identification of pathogenic strains of entomopathogenic nematodes and fungi for wireworm control[J]. Crop Protection, 2009, 28(3):269-272
[18]	张亚波, 吴盼盼, 舒金平.等. 一株绿僵菌的鉴定及其生物学特性研究[J].林业科学, 2012, 48(12):134-140
[19]	周云娥, 白洪青, 舒金平. 筛胸梳爪叩甲生物学特性研究[J].浙江林业科技, 2008, 28(4):28-32

Virulence Bioassay of Different Metarhizium Strains Against Melanotus cribricollis Larvae

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