Preparation of Polyclonal Antibody against Ericerus pela FAR1 Peptide

HU Yan-hong; YANG Pu; CHEN Xiao-ming; XU Dong-li

Fatty acyl-CoA reductase(FAR1) plays a key role in the white wax secretion of Ericerus pela. In present study, the artificially synthesized polypeptide of E. pela FAR1 was used as antigen for the generation of polyclonal antibody. The obtained antibodies were purified by using activated sepharose and analyzed by SDS-PAGE. The concentration and titer of the polyclonal antibody were determined by BCA KIT and ELISA respectively. The results indicated that the titer (1:512000) and the concentration (0.7 mg·mL-1) of anti-FAR1 polyclonal antibody could meet the requirements for later experiments, and the polyclonal antibody could be used to further study the location of FAR1 protein in the later research.

1. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, Jiangsu, China

2. Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, Yunnan, China

Received Date: 2013-08-29

Abstract: Fatty acyl-CoA reductase(FAR1) plays a key role in the white wax secretion of Ericerus pela. In present study, the artificially synthesized polypeptide of E. pela FAR1 was used as antigen for the generation of polyclonal antibody. The obtained antibodies were purified by using activated sepharose and analyzed by SDS-PAGE. The concentration and titer of the polyclonal antibody were determined by BCA KIT and ELISA respectively. The results indicated that the titer (1:512000) and the concentration (0.7 mg·mL-1) of anti-FAR1 polyclonal antibody could meet the requirements for later experiments, and the polyclonal antibody could be used to further study the location of FAR1 protein in the later research.

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Preparation of Polyclonal Antibody against Ericerus pela FAR1 Peptide

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