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Cloning and Sequence Analysis of GAPDH Family Genes from Eucalyptus camaldulensis

  • Received Date: 2013-07-02
  • GAPDH is a typical structural protein in eukaryote organism, which plays an important role involved in the biosynthesis of cell composition and the expression of genetic information. In order to reveal the biological function of GAPDH in the growth and development of Eucalyptus, 3 genes were obtained and named as EC-GAP1, EC-GAP2 and EC-GAP3 by amplification with primers for GAPDH genes conserved regions and RACE method from the young leaf of Eucalyptus camaldulensis. The bioinformatics analysis suggested that the proteins encoded by corresponding genes owned the typical conserved domains of GAPDH and showed high homology with those of other plant species.
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Cloning and Sequence Analysis of GAPDH Family Genes from Eucalyptus camaldulensis

  • 1. China Eucalypt Research Centre, Zhanjiang 524022, Guangdong, China
  • 2. Department of Agronomy-Forestry Science, Huanghuai University, Zhumadian 463000, He'nan, China

Abstract: GAPDH is a typical structural protein in eukaryote organism, which plays an important role involved in the biosynthesis of cell composition and the expression of genetic information. In order to reveal the biological function of GAPDH in the growth and development of Eucalyptus, 3 genes were obtained and named as EC-GAP1, EC-GAP2 and EC-GAP3 by amplification with primers for GAPDH genes conserved regions and RACE method from the young leaf of Eucalyptus camaldulensis. The bioinformatics analysis suggested that the proteins encoded by corresponding genes owned the typical conserved domains of GAPDH and showed high homology with those of other plant species.

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