Analysis of Genetic Difference and Relationship of Camellia meiocarpa Native Varieties by Morphology and AFLP Markers
-
1.
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China
-
2.
Fujian Academy of Forestry Sciences, Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China, State Forestry Administration, Fuzhou 350012, Fujian, China
-
Received Date:
2013-03-05
-
Abstract
The genetic difference of four native Camellia meiocarpa varieties were studied based on their morphological characters and AFLP markers. The results showed that a relatively high level of genetic variation of fruit, leaf and flower traits was detected in the four varieties, and the variation coefficient (CV) of fresh fruit number per 500 g was the largest (76.91%), but that of unsaturated fatty acid was the smallest (3.31%). The fruit characters had higher variation than that of leaf and flower traits. The fruit size (fruit longitudinal diameter × fruit transverse diameter) had significantly or extremely significantly positive correlation with the leaf length, leaf circumference and corolla diameter. And the local variety Yichunbaipi had higher variation than that of the other three varieties. 25 pairs of AFLP primers were selected to amplify the genomic DNAs of the four C. meiocarpa varieties and C. oleifera. A total of 921 AFLP bands were obtained, in which 706 bands were polymorphic makers, and the percentage of polymorphism was 75.7%. The genetic similarity coefficient between the varieties Yangshi and Yichunbaipi was the largest (0.698 2), while that of the variety Longyan and C. oleifera was the smallest(0.558 1). Cluster analysis by unweighted pair-group method with arithmetic mean (UPGMA) showed that C. meiocarpa and C. oleifera could be well distinguished both by AFLP or morphological markers, but the cluster results based on AFLP makers for the four C. meiocarpa varieties were not fully consistent with that based on their morphological characters.
-
-
References
|
[1]
|
庄瑞林. 中国油茶[M]. 2版. 北京: 中国林业出版社, 2008: 65-67
|
|
[2]
|
李振纪. 油茶[M]. 北京: 中国林业出版社, 1981: 5-32
|
|
[3]
|
柏云爱, 宋大海, 张富强, 等. 油茶籽油与橄榄油营养价值的比较[J]. 中国油脂, 2008, 33(3): 39-41
|
|
[4]
|
廖书娟, 吉当玲, 童荣华. 茶油脂肪酸组成及其营养保健功能[J]. 粮食与油脂, 2005(6): 7-9
|
|
[5]
|
熊年康, 郭 江, 陈祥平, 等. 油茶优良农家品种龙眼茶的丰产性状研究[J]. 福建林业科技, 1987(2): 29-36
|
|
[6]
|
庄瑞林, 蔡肖群. 油茶优良农家品种全国区域性评比试验研究[J]. 广西林业科技, 1993, 22(2): 46-53
|
|
[7]
|
黄 勇. 小果油茶遗传多样性分析及杂交渐渗研究. 北京: 中国林业科学研究院, 2011
|
|
[8]
|
姚小华, 王亚萍, 王开良, 等. 地理经纬度对油茶籽中脂肪及脂肪酸组成的影响[J]. 中国油脂, 2011, 36(4): 31-34
|
|
[9]
|
张智俊, 谭晓风, 陈永忠. 油茶总RNA及mRNA的分离与纯化[J]. 中南林学院学报, 2003, 23(2): 76-78
|
|
[10]
|
Bachem C W B, Oomen R J F J, Visser R G F. Transcript imaging with cDNA-AFLP: a step-by-step protocol[J]. Plant Molecular Biology Reporter, 1998, 16(2): 157-173 |
|
[11]
|
Bachem C W B, van der Hoeven R S, de Bruijn S M, et al. Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: analysis of gene expression during potato tuber development[J]. Plant Journal, 1996, 9(5): 745-753 |
|
[12]
|
Rolf F J. NTSYS-pc: Numerical taxonomy and multivariate analysis system. Version 2.1[M]. Setauket, New York: Exeter Software, 2000 |
|
[13]
|
胡先骕. 中国山茶科小志Ⅰ[J]. 科学通报, 1957(6): 170
|
|
[14]
|
张宏达, 任善湘. 中国山茶科新植物[J]. 中山大学学报: 自然科学版, 1992, 31(1): 74-76
|
|
[15]
|
中国科学院中国植物志编辑委员会. 中国植物志:第49卷 第3分册 山茶科(一) 山茶亚科[M]. 北京: 科学出版社, 1998: 13-14
|
|
[16]
|
闵天禄. 世界山茶属的研究[M]. 昆明: 云南科技出版社, 2000: 296-301
|
|
[17]
|
姚小华. 油茶高效实用栽培技术[M]. 北京: 科学出版社, 2010: 1-3
|
|
[18]
|
姚小华, 王开良, 黄 勇, 等. 小果油茶不同居群种仁含油率及脂肪酸组分变异特征分析及评价[J]. 林业科学研究, 2013, 26(5): 533-541
|
-
-
Proportional views
-
DownLoad: