Isolation and Expression Analysis on Vernicia fordii Oleosin Gene of Five VfOLE Isoforms

WU Qing-ke; YANG Su-su; WANG Yang-dong; GAO Ming; CHEN Yi-cun

Plant lipid in oilseeds is generally stored in a dispersive and stable sub-cellular droplet "oil body". And oleosin protein, as the main structural protein of oil body, is responsible for its functional modification. Oleosin plays an important role in the process of oilseed maturation, dormancy and germination. In this study, five oleosin isoforms, named VfOLE1, VfOLE2, VfOLE3, VfOLE4, VfOLE5, were isolated from Vernicia fordii (tung tree). Quantitative real time PCR method was employed to identify the expression mode of oleosin gene of the 5 isoforms, and the results showed that the five VfOLEs were all expressed exclusively in the oilseeds. Furthermore, the rule of the five VfOLEs expression in the tung tree developing seeds was in accordance with those of tung oil and α-eleostearic acid accumulation. In addition, the VfOLE1 and VfOLE2 expression increased immediately and higher than those of the other VfOLEs in tung oil and α-eleostearic acid biosynthesis.

1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China

Received Date: 2013-11-02

Abstract: Plant lipid in oilseeds is generally stored in a dispersive and stable sub-cellular droplet "oil body". And oleosin protein, as the main structural protein of oil body, is responsible for its functional modification. Oleosin plays an important role in the process of oilseed maturation, dormancy and germination. In this study, five oleosin isoforms, named VfOLE1, VfOLE2, VfOLE3, VfOLE4, VfOLE5, were isolated from Vernicia fordii (tung tree). Quantitative real time PCR method was employed to identify the expression mode of oleosin gene of the 5 isoforms, and the results showed that the five VfOLEs were all expressed exclusively in the oilseeds. Furthermore, the rule of the five VfOLEs expression in the tung tree developing seeds was in accordance with those of tung oil and α-eleostearic acid accumulation. In addition, the VfOLE1 and VfOLE2 expression increased immediately and higher than those of the other VfOLEs in tung oil and α-eleostearic acid biosynthesis.

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Reference (21)

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Isolation and Expression Analysis on Vernicia fordii Oleosin Gene of Five VfOLE Isoforms

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