Stigma Receptivity and Characteristics of Pollen Tube Growth of Corylus kweichowensis

HUO Hong-liang; ZHANG Ri-qing; MA Qing-hua; ZHAO Tian-tian; LIANG Li-song; WANG Gui-xi

Volume 27 Issue 3

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Stigma Receptivity and Characteristics of Pollen Tube Growth of Corylus kweichowensis

1.
College of Forestry, Central South University of Forestry &Technology, Changsha 410004, Hu'nan, China
2.
Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, State Key Laboratory of Tree Genetics and Breeding, Beijing 100091, China

Received Date: 2013-11-25

Abstract

This study is to determine the optimum receptive period of the stigma, the feasible pollination method, the suitable pollination environment for the cross breeding of Corylus. Taking C. kweichowensis as experiment material, C. heterophylla and C. heterophylla × C. avellana as control groups, the female inflorescences were pollinated with mixed pollen of C. kweichowensis, C. heterophylla and C. heterophylla × C. avellana at different blooming periods, using different pollination methods and under different environments. The pollinated female inflorescences were excised at different time, the pollen adhesion number, germination rate and the pollen tube were observed by fluorescent microscopy. The results showed that the descending order of the stigma receptivity of C. kweichowensis in different blooming periods was florescence period > terminal florescence period > red dot period. The stigma receptivity of loose inflorescences was higher than that of the straight cluster inflorescences. The method of parallel brush pollination was more efficient than the method of point pollination. When the pollinated inflorescences were incubated in a petri dish with double-layer wet filter paper, both the germination and growth speeds of pollen tubes were faster than that in the greenhouse and the field, indicating that humid and mild environment was more suitable for the adhesion and germination of the pollen, as well as the pollen tube growth of C. kweichowensis. The temperature and humidity and the germplasm of the pollinated inflorescences had important effects on the germination of the pollen. The speed of pollen tube growth was strongly influenced by environmental temperature and humidity, but had nothing to do with germplasm.
- Corylus kweichowensis,
- blooming period,
- inflorescence morphology,
- stigma receptivity,
- pollen tube,
- fluorescence microscopy observation

References

[1]	张宇和, 柳鎏, 梁维坚, 等. 中国果树志·板栗: 榛子卷[M]. 北京: 中国林业出版社, 2005: 190-200
[2]	Rahemi M, Mojadad D. Effect of pollen source on nut and kernel characteristics of hazelnut[J]. Acta Hort, 2001, 556: 371-376
[3]	Dafni A. Pollination Ecology, a Practical Approach[M]. New York: Oxford University Press, 1992:59–89
[4]	叶要妹, 张俊卫, 齐迎春. 百日草柱头可授性和花粉生活力的研究[J]. 中国农业科学, 2007, 40(10): 2376-2381
[5]	赵剑颖, 张华丽, 张西西. 万寿菊花粉活力及柱头可授性研究[J]. 中国农学通报, 2012, 28(19): 159-163
[6]	马春英, 屈平, 王文全. 甘草花粉超微鉴定及花粉活力、柱头可授性荧光显微镜观察[J]. 植物遗传资源学报, 2011, 12(3): 396-401
[7]	律春燕, 王雁, 朱向涛. 黄牡丹与芍药组间杂交花粉与柱头识别的解剖学研究[J]. 西北植物学报, 2009, 29(10): 1988-1994
[8]	杨芩, 付燕, 王永清. 阴雨对'大五星’枇杷柱头可授性和花粉活力的影响[J]. 北方园艺, 2011, 86(3): 221-224
[9]	王其刚, 张颢, 蹇洪英. 月季柱头形态发育进程与可授性[J]. 西北农业学报, 2012, 21(6): 169-173
[10]	刘宁. 花柱和柱头的结构[J]. 生物学通报, 1998, 33(4): 15-17
[11]	刘剑锋, 颜堃, 程云清, 等. 榛子花粉生活力和柱头可授性与结实特征研究[J]. 北京林业大学学报, 2012, 34(3): 58-63
[12]	Mehlenbacher S A. Chilling requirements of hazelnut cultivars[J]. Scientia Horticulturae, 2001, 47: 271-282
[13]	王君, 康向阳, 刘立成,等. 青杨派树种雌蕊柱头可授性及最佳授粉时期[J]. 北京林业大学学报, 2009, 31(4): 30-35
[14]	Hampson C R, Azarenko A N, Soleldner A. Pollen-stigma interactions following compatible and incompatible pollinations in hazelnut[J]. Horticultural Science, 1993, 118: 814-819
[15]	Thompson M M. Genetics of incompatibility in Corylus avellana L.[J].Theoretical and Applied Genetics, 1979(54): 133-116
[16]	Germain E. The reproduction of hazelnut (Corylus avellana L.) : A review[J]. Acta Horticulturae, 1994, 351: 195-210
[17]	Thompson M M. Incompatibility alleles in Corylus avellana cultivars[J].Theoretical and Applied Genetics, 1979, 54: 29-33
[18]	Fabrizio, Ciampolini. The structure and cytochemistry of the stigma-style complex of Corylus avellana L.‘Tonda Gentile delle Langhe’ (Corylaceae) [J]. Annals of Botany,1998, 81: 513-518
[19]	Cheryl R, Hampson, Anita N A. Pollen-Stigma Interactions following Compatible and Incompatible Pollinations in Hazelnut[J]. Journal of the American Society for Horticultural,1993, 118(6): 814-819
[20]	李艳华, 康向阳. 白杨派树种雌蕊柱头可授性及其检测方法的研究[J]. 西北植物学报, 2007, 27(5): 859-863
[21]	刘林德, 张洪军, 祝宁, 等. 刺五加花粉活力和柱头可授性的研究[J]. 植物研究, 2001, 21(3): 375-379
[22]	徐汉卿. 植物学[M]. 北京: 中国农业出版社, 1996:176-198
[23]	李合生. 现代植物生理学(第二版)[M]. 北京: 高等教育出版社, 2001: 305-310
[24]	Heslop-Harrison J. Germination of Corylus avellana L. (hazel) pollen: hydration and the function of the oncus[J]. Acta Botanica Neerlandica, 1986, 35: 265-284
[25]	Hampson C R, Azarenko A N, Soeldner A. Pollen-stigma interactions following compatible and incompatible pollinations in hazelnut[J]. Journal of the American Society for Horticultural, 1993, 118: 814-819
[26]	Mehlenbacher S A. Testing compatibility of hazelnut crossesusing using fluorescence microscopy[J]. ISHS Acta Horticulturae, 1997, 445:167-172

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Stigma Receptivity and Characteristics of Pollen Tube Growth of Corylus kweichowensis

1. College of Forestry, Central South University of Forestry &Technology, Changsha 410004, Hu'nan, China

2. Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, State Key Laboratory of Tree Genetics and Breeding, Beijing 100091, China

Received Date: 2013-11-25

Abstract: This study is to determine the optimum receptive period of the stigma, the feasible pollination method, the suitable pollination environment for the cross breeding of Corylus. Taking C. kweichowensis as experiment material, C. heterophylla and C. heterophylla × C. avellana as control groups, the female inflorescences were pollinated with mixed pollen of C. kweichowensis, C. heterophylla and C. heterophylla × C. avellana at different blooming periods, using different pollination methods and under different environments. The pollinated female inflorescences were excised at different time, the pollen adhesion number, germination rate and the pollen tube were observed by fluorescent microscopy. The results showed that the descending order of the stigma receptivity of C. kweichowensis in different blooming periods was florescence period > terminal florescence period > red dot period. The stigma receptivity of loose inflorescences was higher than that of the straight cluster inflorescences. The method of parallel brush pollination was more efficient than the method of point pollination. When the pollinated inflorescences were incubated in a petri dish with double-layer wet filter paper, both the germination and growth speeds of pollen tubes were faster than that in the greenhouse and the field, indicating that humid and mild environment was more suitable for the adhesion and germination of the pollen, as well as the pollen tube growth of C. kweichowensis. The temperature and humidity and the germplasm of the pollinated inflorescences had important effects on the germination of the pollen. The speed of pollen tube growth was strongly influenced by environmental temperature and humidity, but had nothing to do with germplasm.

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Reference (26)

[1]	张宇和, 柳鎏, 梁维坚, 等. 中国果树志·板栗: 榛子卷[M]. 北京: 中国林业出版社, 2005: 190-200
[2]	Rahemi M, Mojadad D. Effect of pollen source on nut and kernel characteristics of hazelnut[J]. Acta Hort, 2001, 556: 371-376
[3]	Dafni A. Pollination Ecology, a Practical Approach[M]. New York: Oxford University Press, 1992:59–89
[4]	叶要妹, 张俊卫, 齐迎春. 百日草柱头可授性和花粉生活力的研究[J]. 中国农业科学, 2007, 40(10): 2376-2381
[5]	赵剑颖, 张华丽, 张西西. 万寿菊花粉活力及柱头可授性研究[J]. 中国农学通报, 2012, 28(19): 159-163
[6]	马春英, 屈平, 王文全. 甘草花粉超微鉴定及花粉活力、柱头可授性荧光显微镜观察[J]. 植物遗传资源学报, 2011, 12(3): 396-401
[7]	律春燕, 王雁, 朱向涛. 黄牡丹与芍药组间杂交花粉与柱头识别的解剖学研究[J]. 西北植物学报, 2009, 29(10): 1988-1994
[8]	杨芩, 付燕, 王永清. 阴雨对'大五星’枇杷柱头可授性和花粉活力的影响[J]. 北方园艺, 2011, 86(3): 221-224
[9]	王其刚, 张颢, 蹇洪英. 月季柱头形态发育进程与可授性[J]. 西北农业学报, 2012, 21(6): 169-173
[10]	刘宁. 花柱和柱头的结构[J]. 生物学通报, 1998, 33(4): 15-17
[11]	刘剑锋, 颜堃, 程云清, 等. 榛子花粉生活力和柱头可授性与结实特征研究[J]. 北京林业大学学报, 2012, 34(3): 58-63
[12]	Mehlenbacher S A. Chilling requirements of hazelnut cultivars[J]. Scientia Horticulturae, 2001, 47: 271-282
[13]	王君, 康向阳, 刘立成,等. 青杨派树种雌蕊柱头可授性及最佳授粉时期[J]. 北京林业大学学报, 2009, 31(4): 30-35
[14]	Hampson C R, Azarenko A N, Soleldner A. Pollen-stigma interactions following compatible and incompatible pollinations in hazelnut[J]. Horticultural Science, 1993, 118: 814-819
[15]	Thompson M M. Genetics of incompatibility in Corylus avellana L.[J].Theoretical and Applied Genetics, 1979(54): 133-116
[16]	Germain E. The reproduction of hazelnut (Corylus avellana L.) : A review[J]. Acta Horticulturae, 1994, 351: 195-210
[17]	Thompson M M. Incompatibility alleles in Corylus avellana cultivars[J].Theoretical and Applied Genetics, 1979, 54: 29-33
[18]	Fabrizio, Ciampolini. The structure and cytochemistry of the stigma-style complex of Corylus avellana L.‘Tonda Gentile delle Langhe’ (Corylaceae) [J]. Annals of Botany,1998, 81: 513-518
[19]	Cheryl R, Hampson, Anita N A. Pollen-Stigma Interactions following Compatible and Incompatible Pollinations in Hazelnut[J]. Journal of the American Society for Horticultural,1993, 118(6): 814-819
[20]	李艳华, 康向阳. 白杨派树种雌蕊柱头可授性及其检测方法的研究[J]. 西北植物学报, 2007, 27(5): 859-863
[21]	刘林德, 张洪军, 祝宁, 等. 刺五加花粉活力和柱头可授性的研究[J]. 植物研究, 2001, 21(3): 375-379
[22]	徐汉卿. 植物学[M]. 北京: 中国农业出版社, 1996:176-198
[23]	李合生. 现代植物生理学(第二版)[M]. 北京: 高等教育出版社, 2001: 305-310
[24]	Heslop-Harrison J. Germination of Corylus avellana L. (hazel) pollen: hydration and the function of the oncus[J]. Acta Botanica Neerlandica, 1986, 35: 265-284
[25]	Hampson C R, Azarenko A N, Soeldner A. Pollen-stigma interactions following compatible and incompatible pollinations in hazelnut[J]. Journal of the American Society for Horticultural, 1993, 118: 814-819
[26]	Mehlenbacher S A. Testing compatibility of hazelnut crossesusing using fluorescence microscopy[J]. ISHS Acta Horticulturae, 1997, 445:167-172

Stigma Receptivity and Characteristics of Pollen Tube Growth of Corylus kweichowensis

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Stigma Receptivity and Characteristics of Pollen Tube Growth of Corylus kweichowensis

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