Genome Size Determination of Several Dendrolimus Species in China

ZHANG Su-fang; ZHANG Zhen; WANG Hong-bin; KONG Xiang-bo; LUO Ji-tong; YANG Zhong-wu

Volume 27 Issue 5

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Genome Size Determination of Several Dendrolimus Species in China

1.
Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China
2.
Forest pest and disease control station of the Guangxi Zhuang Autonomous Region, Nanning 530022, Guangxi, China
3.
Forest pest and disease control station of Guilin city, the Guangxi Zhuang Autonomous Region, Guilin 541001, China

Received Date: 2013-12-06

Abstract

The genome size of three important Dendrolimus species in South China were measured by flow cytometry, with chicken erythrocyte DNA content as control. The results showed that the genome size of D. punctatus was 563.36±7.26 Mb, and the 2C DNA content of female and male were 1.1756±0.0164 and 1.1286±0.0134 pg, respectively. The genome size of D. kikuchii was 719.30±9.70 Mb, and the 2C DNA content of female and male were 1.4492±0.0212 and 1.4428±0.0184 pg, respectively. The genome size of D. houi was 586.82±8.94 Mb, and the 2C DNA content of female and male were 1.244±0.0182 and 1.156±0.0184 pg, respectively. Statistical analysis indicated that the genome size of D. kikuchii was much greater than that of D. houi and D. punctatus, and the genome size of D. houi was a little larger than that of the D. punctatus. For all the three species, the genome size of the female was larger than that of the male. Genome size determinations of these Dendrolimus species can be used as the basis of further comparison among the molecular differentiation of these closely related and sympatric species, and also provide important information for genomic library construction and sequencing in the future.
- flow cytometry,
- genome size,
- Dendrolimus,
- chicken erythrocyte

References

[1]	陈昌洁. 松毛虫综合管理[M]. 北京: 中国林业出版社, 1990.
[2]	萧刚柔. 中国森林昆虫[M]. 北京: 中国林业出版社, 1992.
[3]	侯陶谦. 中国松毛虫[M]. 北京: 科学出版社, 1987.
[4]	李天生. 松毛虫监测与防治方法研究进展[J]. 昆虫知识, 2000,37(2): 122-128.
[5]	Kong X-B, Liu K-W, Wang H-B, et al. Identification and Behavioral Evaluation of Sex Pheromone Components of the Chinese Pine Caterpillar Moth, Dendrolimus tabulaeformis[J]. PLoS ONE, 2012,7(3): e33381.
[6]	孔祥波, 赵成华, 高伟. 4种松毛虫性信息素成分及其在近缘种生殖隔离中的作用[J]. 科学通报, 2001,46(17): 1435-1439.
[7]	张真, 李典谟, 查光济. 马尾松毛虫种群动态的时间序列分析及复杂性动态研究[J]. 生态学报, 2002,7: 1061-1067.
[8]	张爱兵, 孔祥波, 李典谟, 等. 中国松毛虫属八个种和亚种亲缘关系的DNA指纹证据[J]. 昆虫学报, 2004,47(2): 236-242.
[9]	Zhang S F, Zhang Z, Kong X B, et al. Molecular characterization and phylogenetic analysis of three odorant binding protein gene transcripts in Dendrolimus (Lepidoptera: Lasiocampidae)[J]. Insect Science: n/a-n/a. DOI: 10.1111/1744-7917.12074
[10]	Doležel J, Bartos J, Voglmayr H, et al. Nuclear DNA content and genome size of trout and human[J]. Cytometry A, 2003,51(2): 127-128.
[11]	Gregory T R. The Bigger the C-Value, the Larger the Cell: Genome Size and Red Blood Cell Size in Vertebrates[J]. Blood Cells, Molecules, and Diseases, 2001,27(5): 830-843.
[12]	Griffith O L, Moodie G E E, Civetta A. Genome size and longevity in fish[J]. Experimental Gerontology, 2003,38(3): 333-337.
[13]	Vinogradov A E. Genome size and extinction risk in vertebrates[J]. Proceedings of the Royal Society of London Series B: Biological Sciences, 2004,271(1549): 1701-1705.
[14]	Vinogradov A E. Selfish DNA is maladaptive: evidence from the plant Red List[J]. Trends in Genetics, 2003,19(11): 609-614.
[15]	薛建, 程家安, 张传溪. 昆虫基因组及其大小[J]. 昆虫学报, 2009,52(8): 901-906.
[16]	Boulesteix M, Weiss M, Biémont* C. Differences in Genome Size Between Closely Related Species: The Drosophila melanogaster Species Subgroup[J]. Molecular Biology and Evolution, 2006,23(1): 162-167.
[17]	Petrov D A. Evolution of genome size: new approaches to an old problem[J]. Trends in Genetics, 2001,17(1): 23-28.
[18]	Birstein V J, Poletaev A I, Goncharov B F. DNA content in eurasian sturgeon species determined by flow cytometry[J]. Cytometry, 1993,14(4): 377-383.
[19]	Johnston J S, Bennett M D, Rayburn A L, et al. Reference standards for determination of DNA content of plant nuclei[J]. American Journal of Botany, 1999,86(5): 609-613.
[20]	Gregory T R. Animal Genome Size Database. [M]. 2005.
[21]	左连富, 王风荣. 流式细胞术样品制备技术[M]. 北京: 华夏出版社, 1991.
[22]	group Ba, Xia Q, Zhou Z, et al. A Draft Sequence for the Genome of the Domesticated Silkworm (Bombyx mori)[J]. Science, 2004,306(5703): 1937-1940.
[23]	Thomas C A. The Genetic Organization of Chromosomes[J]. Annual Review of Genetics, 1971,5(1): 237-256.
[24]	蔡邦华, 候?瘦漬氠疋瓫榎漮渠慾狛祫??槴潂沤濊杂种???や〝?????????扫牦?嬬??崹??爬攱朴漨爴礩?吠″刴???猵?献洼慢汲氾?椲渵摝攠汵?扱椬愠玁?感?搠敾瓛敫犄浂榤湗愠滕璌?潊晝?朠攆湫潦涥攬?猱椹稹攲?嬨?崩??吲爸攭渳搲献?楢湲￣?攲渶敝琠楗掫珪??㈠ヘゝ??ㄠ??????????????松毛虫不同地理种群RAPD遗传多样性分析[J]. 农业生物技术学报, 2009,17(1): 178-179.
[25]	南宫自艳, 高宝嘉, 刘军侠. 四种松毛虫不地理种群遗传多样性的等位酶分析[J]. 昆虫学报, 2008,51(4): 417-423.
[26]	Tan Q, Yan X F, Wen J B, et al. Phylogenetic relationship of seven Dendrolimus (Lepidoptera: Lasiocampidae) species based on the ultrastructure of male moths' antennae and antennal sensilla[J]. Microscopy Research and Technique, 2012,75(12): 1700-1710.
[27]	Grime J, Mowforth M. Variation in genome size-an ecological interpretation[J]. Nature, 1982,299: 151-153.
[28]	Gregory T R. Nucleotypic effects without nuclei: genome size and erythrocyte size in mammals.[J]. Genome, 2000,43: 895-901.
[29]	Reeves G, Francis D, Davies M, et al. Genome size is negatively correlated with altitude in natural populations of Dactylis glomerata[J]. Annals of Botany, 1998,82: 99-105.
[30]	Charles A, David D. Variation in nuclear DNA content across environmental gradients: a quantile regression analysis[J]. Ecology Letters, 2002,5: 66-76.
[31]	Hall S, Dvorak W, Johnston JS, et al. Flow cytometric analysis of DNA content for tropical and temperate new world pines[J]. Annals of Botany, 2000,86: 1081-1086.
[32]	Vinogradov A. Larger Genomes for molluskan land pioneers[J]. Genome, 2000,43: 211-212.
[33]	Ebeling A, Atkin N, Setzer P. Genome sizes of teleostean fishes: increase in some deep-sea species[J]. American Naturalist, 1971,105: 549-561.
[34]	Vieira C, Nardon C, Arpin C, et al. Evolution of genome size in Drosophila in the invader's genome being invaded by transposable element?[J]. Molecular Biology and Evolution, 2002,19(7): 1154-1161.
[35]	Finston T, Hebert P, Foottit R. Genome size variation in aphids[J]. Insect Biochemistry and Molecular Biology, 1995,25(2): 189-196.
[36]	Tsutsui N, Suarez A, Spagna J, et al. The evolution of genome size in ants[J]. BMC

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Genome Size Determination of Several Dendrolimus Species in China

1. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing 100091, China

2. Forest pest and disease control station of the Guangxi Zhuang Autonomous Region, Nanning 530022, Guangxi, China

3. Forest pest and disease control station of Guilin city, the Guangxi Zhuang Autonomous Region, Guilin 541001, China

Received Date: 2013-12-06

Abstract: The genome size of three important Dendrolimus species in South China were measured by flow cytometry, with chicken erythrocyte DNA content as control. The results showed that the genome size of D. punctatus was 563.36±7.26 Mb, and the 2C DNA content of female and male were 1.1756±0.0164 and 1.1286±0.0134 pg, respectively. The genome size of D. kikuchii was 719.30±9.70 Mb, and the 2C DNA content of female and male were 1.4492±0.0212 and 1.4428±0.0184 pg, respectively. The genome size of D. houi was 586.82±8.94 Mb, and the 2C DNA content of female and male were 1.244±0.0182 and 1.156±0.0184 pg, respectively. Statistical analysis indicated that the genome size of D. kikuchii was much greater than that of D. houi and D. punctatus, and the genome size of D. houi was a little larger than that of the D. punctatus. For all the three species, the genome size of the female was larger than that of the male. Genome size determinations of these Dendrolimus species can be used as the basis of further comparison among the molecular differentiation of these closely related and sympatric species, and also provide important information for genomic library construction and sequencing in the future.

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Reference (36)

[1]	陈昌洁. 松毛虫综合管理[M]. 北京: 中国林业出版社, 1990.
[2]	萧刚柔. 中国森林昆虫[M]. 北京: 中国林业出版社, 1992.
[3]	侯陶谦. 中国松毛虫[M]. 北京: 科学出版社, 1987.
[4]	李天生. 松毛虫监测与防治方法研究进展[J]. 昆虫知识, 2000,37(2): 122-128.
[5]	Kong X-B, Liu K-W, Wang H-B, et al. Identification and Behavioral Evaluation of Sex Pheromone Components of the Chinese Pine Caterpillar Moth, Dendrolimus tabulaeformis[J]. PLoS ONE, 2012,7(3): e33381.
[6]	孔祥波, 赵成华, 高伟. 4种松毛虫性信息素成分及其在近缘种生殖隔离中的作用[J]. 科学通报, 2001,46(17): 1435-1439.
[7]	张真, 李典谟, 查光济. 马尾松毛虫种群动态的时间序列分析及复杂性动态研究[J]. 生态学报, 2002,7: 1061-1067.
[8]	张爱兵, 孔祥波, 李典谟, 等. 中国松毛虫属八个种和亚种亲缘关系的DNA指纹证据[J]. 昆虫学报, 2004,47(2): 236-242.
[9]	Zhang S F, Zhang Z, Kong X B, et al. Molecular characterization and phylogenetic analysis of three odorant binding protein gene transcripts in Dendrolimus (Lepidoptera: Lasiocampidae)[J]. Insect Science: n/a-n/a. DOI: 10.1111/1744-7917.12074
[10]	Doležel J, Bartos J, Voglmayr H, et al. Nuclear DNA content and genome size of trout and human[J]. Cytometry A, 2003,51(2): 127-128.
[11]	Gregory T R. The Bigger the C-Value, the Larger the Cell: Genome Size and Red Blood Cell Size in Vertebrates[J]. Blood Cells, Molecules, and Diseases, 2001,27(5): 830-843.
[12]	Griffith O L, Moodie G E E, Civetta A. Genome size and longevity in fish[J]. Experimental Gerontology, 2003,38(3): 333-337.
[13]	Vinogradov A E. Genome size and extinction risk in vertebrates[J]. Proceedings of the Royal Society of London Series B: Biological Sciences, 2004,271(1549): 1701-1705.
[14]	Vinogradov A E. Selfish DNA is maladaptive: evidence from the plant Red List[J]. Trends in Genetics, 2003,19(11): 609-614.
[15]	薛建, 程家安, 张传溪. 昆虫基因组及其大小[J]. 昆虫学报, 2009,52(8): 901-906.
[16]	Boulesteix M, Weiss M, Biémont* C. Differences in Genome Size Between Closely Related Species: The Drosophila melanogaster Species Subgroup[J]. Molecular Biology and Evolution, 2006,23(1): 162-167.
[17]	Petrov D A. Evolution of genome size: new approaches to an old problem[J]. Trends in Genetics, 2001,17(1): 23-28.
[18]	Birstein V J, Poletaev A I, Goncharov B F. DNA content in eurasian sturgeon species determined by flow cytometry[J]. Cytometry, 1993,14(4): 377-383.
[19]	Johnston J S, Bennett M D, Rayburn A L, et al. Reference standards for determination of DNA content of plant nuclei[J]. American Journal of Botany, 1999,86(5): 609-613.
[20]	Gregory T R. Animal Genome Size Database. [M]. 2005.
[21]	左连富, 王风荣. 流式细胞术样品制备技术[M]. 北京: 华夏出版社, 1991.
[22]	group Ba, Xia Q, Zhou Z, et al. A Draft Sequence for the Genome of the Domesticated Silkworm (Bombyx mori)[J]. Science, 2004,306(5703): 1937-1940.
[23]	Thomas C A. The Genetic Organization of Chromosomes[J]. Annual Review of Genetics, 1971,5(1): 237-256.
[24]	蔡邦华, 候?瘦漬氠疋瓫榎漮渠慾狛祫??槴潂沤濊杂种???や〝?????????扫牦?嬬??崹??爬攱朴漨爴礩?吠″刴???猵?献洼慢汲氾?椲渵摝攠汵?扱椬愠玁?感?搠敾瓛敫犄浂榤湗愠滕璌?潊晝?朠攆湫潦涥攬?猱椹稹攲?嬨?崩??吲爸攭渳搲献?楢湲￣?攲渶敝琠楗掫珪??㈠ヘゝ??ㄠ??????????????松毛虫不同地理种群RAPD遗传多样性分析[J]. 农业生物技术学报, 2009,17(1): 178-179.
[25]	南宫自艳, 高宝嘉, 刘军侠. 四种松毛虫不地理种群遗传多样性的等位酶分析[J]. 昆虫学报, 2008,51(4): 417-423.
[26]	Tan Q, Yan X F, Wen J B, et al. Phylogenetic relationship of seven Dendrolimus (Lepidoptera: Lasiocampidae) species based on the ultrastructure of male moths' antennae and antennal sensilla[J]. Microscopy Research and Technique, 2012,75(12): 1700-1710.
[27]	Grime J, Mowforth M. Variation in genome size-an ecological interpretation[J]. Nature, 1982,299: 151-153.
[28]	Gregory T R. Nucleotypic effects without nuclei: genome size and erythrocyte size in mammals.[J]. Genome, 2000,43: 895-901.
[29]	Reeves G, Francis D, Davies M, et al. Genome size is negatively correlated with altitude in natural populations of Dactylis glomerata[J]. Annals of Botany, 1998,82: 99-105.
[30]	Charles A, David D. Variation in nuclear DNA content across environmental gradients: a quantile regression analysis[J]. Ecology Letters, 2002,5: 66-76.
[31]	Hall S, Dvorak W, Johnston JS, et al. Flow cytometric analysis of DNA content for tropical and temperate new world pines[J]. Annals of Botany, 2000,86: 1081-1086.
[32]	Vinogradov A. Larger Genomes for molluskan land pioneers[J]. Genome, 2000,43: 211-212.
[33]	Ebeling A, Atkin N, Setzer P. Genome sizes of teleostean fishes: increase in some deep-sea species[J]. American Naturalist, 1971,105: 549-561.
[34]	Vieira C, Nardon C, Arpin C, et al. Evolution of genome size in Drosophila in the invader's genome being invaded by transposable element?[J]. Molecular Biology and Evolution, 2002,19(7): 1154-1161.
[35]	Finston T, Hebert P, Foottit R. Genome size variation in aphids[J]. Insect Biochemistry and Molecular Biology, 1995,25(2): 189-196.
[36]	Tsutsui N, Suarez A, Spagna J, et al. The evolution of genome size in ants[J]. BMC

Genome Size Determination of Several Dendrolimus Species in China

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通讯作者: 陈斌, bchen63@163.com

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Genome Size Determination of Several Dendrolimus Species in China

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