Analysis on Differences of Protein in Stylar Canal During Early Pollination Associated with Self-incompatible Prunus domestica &#215; armeniaca ‘Fengweimeigui’

HE Xing-bo; LEI Li-li; YANG Shao-bin; FU Jian-min; LI Fang-dong

Volume 27 Issue 5

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Analysis on Differences of Protein in Stylar Canal During Early Pollination Associated with Self-incompatible Prunus domestica × armeniaca ‘Fengweimeigui’

1.
Paulownia R&D Center of China, State Forestry Administration, Zhengzhou 450003, Henan, China
2.
Non-timber Forestry R&D Center, Chinese Academy of Forestry, Zhengzhou 450003, Henan, China

Received Date: 2014-01-06

Abstract

This study focused on the differences of protein in stylar canal between that from self-incompatible Prunus domestica × armeniaca ‘Fengweimeigui’ and that from cross-pollination (ACP) (‘Fengweimeigui’ × ‘Konglongdan’). Two dimension difference gel electrophoresis (2D-DIGE) and mass spectrum (MS) detection techniques were used in this study. The results showed that there were 79 different protein spots in stylar canal between that from self-pollination and that from cross-pollination, and there was a huge difference, more than double, in terms of protein expression among 43 protein spots, including 6 up-expressed and 37 down-expressed in self-pollination stylar canal compared with that in cross-pollination stylar canal. Only 34 protein spots were identified unambiguously through mass spectrometry analysis, including 5 kinds which were catalytic protein, defence/stress protein, and structural protein, respectively.
- Prunus domestica×,
- armeniaca ‘Fengweimeigui’,
- self-incompatibility,
- stigma,
- proteomics

References

[1]	李芳东,杜红岩,杨绍彬,等. ‘风味玫瑰'—极早熟杏李杂交新品种[J].中国果树,2007,(3):91-96.
[2]	?27] 杨绍彬,李芳东,杜红岩,等.杏李种间杂交品种适宜授粉组合筛选试验[J].中国果树,2008(5):28-30.
[3]	李芳东,杜红岩,杨绍彬,等.杏李种间杂交极早熟新品种风味玫瑰[J].中国果树,2007,(3):5-7.
[4]	王熙龙,曹振强.风味玫瑰杏李早期丰产栽培技术[J]. 北方果树,2005(4):29.
[5]	李小远,马玉磊,刘阳,等.小报春不同授粉组合亲和性研究[J].西北植物学报,2012,32(12):2426-2431.
[6]	雷家军,吴超.卷丹与亚洲百合种间杂交花粉管生长的荧光观察[J].东北农业大学学报,2012,43(1):117-120.
[7]	罗春香,缑红星,盛瑞芳,等.杏李杂交种风味玫瑰引种表现及主要栽培技术[J].山西果树,2011,1:14-15.
[8]	陈超,李保国,齐国辉,等.果树自交不亲和机理及应用研究进展. 河北林果研究,2005,20(1):86-90.
[9]	吴燕,陈学森,冯建荣,等. 杏杂种一代群体S 基因的遗传研究. 园艺学报,2005,32(4):397-402.
[10]	Silva N F, Goring D R. Mechanisms of self-incompatibility in flowering plants[J]. Cellular and Molecular Life Sciences,2001,58: 1988-2007.
[11]	Foster E, Levesque-Lemay M, Schneiderman D,et al. Characterization of a gene highly expressed in the Brassica napus pistil that encodes a novel proline-rich protein[J]. Sex Plant Reprod, 2005,17:261-267.
[12]	Wang X, Zafian P, Choudhary M,et al. The PR5K receptor protein kinase from Arabidopsis thaliana is structurally related to a family of plant defense proteins[J]. Proceedings of the National Academy of Sciences of the USA, 1996, 93:2598-2602.
[13]	Furuyama T, Dzelzkalns V A. A novel calcium-binding protein is expressed in brassica pistils and anthers late in flower development[J]. Plant Molecular Biology,1999,39:729-737.
[14]	Fukai E, Nishio T, Nasrallah M. Molecular genetic analysis of the candidate gene for MOD, a locus required for self-incompatibility in Brassica rapa[J]. Molecular Genetics and Genomics,2001,265:519-525.
[15]	Sassa H, Hirano H. Style-specific and developmentally regulated accumulation of a glycosylated thaumatin/PR5-like protein in Japanese pear (Pyrus serotina Rehd.)[J]. Planta,1998,205:514-521.
[16]	De Graaf B H J, Knuiman B A, Derksen J, et al. Characterization and localization of the transmitting tissue-specific PELPIII proteins of Nocotiana tabacum[J]. Journal of Experimental Botany, 2003, 54(380):55-63.
[17]	De Graaf B H J, Knuiman B A, van Der Weerden G M, et al. The PELPIII glycoprotein's in Solanaceae : stylar expression and transfer into pollen tube walls[J]. Sex Plant Reprod,2004,16:245-252.
[18]	Expression of an isoflavone reductase-like gene enhanced by pollen tube growth in pistils of Solanum tuberosum[J]. Plant Molecular Biology,1997,33:923-929.
[19]	O'Brien M, Bertrand C, Matton D P. Characterization of a fertilization-induced and developmentally regulated plasma- membrane aquaporin expressed in reproductive tissue, in the wild potato Solanum chacoense Bitt[J]. Planta,2002,215: 485-493.
[20]	Kuboyama T. A novel thaumatin-like protein gene of tobacco is specifically expressed in the transmitting tissue of stigma and style[J]. Sex Plant Reprod,1998,11:251-256.
[21]	Luu D T, Marty-Mazars D, Trick M, et al. Pollen-stigma adhesion in Brassica spp involves SLG and SLR1 glycoproteins[J]. Plant Cell, 1999,11(2):251-262.
[22]	Doughty J, Dixon S, Hiscock S J, et al. PCP-A1, a defensin-like Brassica pollen coat protein that binds the S locus glycoprotein, is the product of gametophytic gene expression[J]. Plant Cell, 1998, 10(8):1333-1347.
[23]	Samuel M A, Chong Y T, Haasen K E, et al. Cellular pathways regulating responses to compatible and self-incompatible pollen in Brassica and Arabidopsis stigmas intersect at Exo70A1, a putative component of the exocyst complex[J]. Plant Cell,2009,21 (9):2655-2671.
[24]	Dai S, Chen T, Chong K, et al. Proteomics identification of differentially expressed associated with pollen germination and tube growth reveals characteristics of germinated Oryza sativa pollen[J]. Mol Cell Proteomics,2007,6 (2):207-230.
[25]	Sheoran I S, Pederson E J, Ross A R, et al. Dynamics of protein expression during pollen germination in canola(Brassica napus)[J]. Planta, 2009,230 (4):779-793.
[26]	Wang Z, Wan g Z, Shi L,et al. Proteomic alterations of Brassica napus root in response to boron deficiency[J]. Plant Mol Biol,2010, 74(3):265-278.
[27]	Yao Y, Sun H, Xu F, et al. Comparative proteome analysis of metabolic changes by low phosphorus stress in two Brassica napus genotypes[J]. Planta, 2011, 233(3):523-537.
[28]	郑洲,陈学森,冯宝春,等.杏品种授粉生物学研究[J].果树学报,2004,21(6):327-331.
[29]	张绍铃,房经贵,杨记磙.果树自交不亲和性的遗传与生理机制及其研究[J].果树学报,2001,18(1):49-52.
[30]	Pieton JM, Steer MW. A model for the mechanism of tip extension in pollen tubes [J]. Journal of Theoretical Biology,1982, 98: 15-20.
[31]	Pierson ES. Rhodamine-phalloidin staining of F-actin in pollen after dimethysulphoxide permeabilization[J]. Sex Plant Reproduction, 1988,1:83-87.
[32]	Lazzaro M D micrdilaments. The spormatogenous body cell of the conifer Picea abies contains actin Protoplasma[J]. 1998, 201:194-201.
[33]	张鹏.杨树杂交不亲和性的细胞学及生理生化基础.北京:北京林业大学,2013.
[34]	Brewbaker J L, Kwack B H. The essential role of calcium ion in pollen germination and pollen tube growth[J]. Am J Bot, 1963, 50 (9): 859-865.
[35]	Rathore K S, Cork R J, Robinson K R. A cytoplasmic gradient of Ca²⁺ is correlated with the growth of lily pollen tubes[J]. Dev Biol, 1991, (148):612-619.
[36]	Gu Y.Q, Vernoud V, Fu Y, et al. ROPgTPase regulation of pollen tubegrowth through the dynamics of tip-localized F-actin[J]. J. Exp. Bot,2003,(54):93-10.
[37]	尚桂军. TteRRF, AmCBL1初步晶体学和嗜热菌ATCC27502-ATP合酶晶体生长研究.北京:北京林业大学. 2010.
[38]	Okuda S, Tsutsui H, Shiina K, et al. Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells[J]. nature, 458 (7236):357-361.
[39]	Amien S, Kliwer I, Márton M L, et al. Defensin-like ZmES 4 mediates pollen tube burst in maizevia opening of the potassium channel KZM1[J]. PLoS Biol, 2010, 8 (6):e1000388.
[40]	Kessler S A, Shimosato-Asano H, Keinath N F, et al. Conserved molecular components for pollen tube reception and fungal invasion[J]. Science, 2010, 330:968-971.
[41]	张水军,曾千春,卢秀萍,等.植物富含甘氨酸蛋白的研究进展[J].中国农学通报,2010,26(4):54-58.
[42]	孙培亮,王志亮,李金明,等.热休克蛋白70研究新进展[J].中国兽医学报,2007,27(2):284-288.
[43]	Lehmann S, Funck D, Szabados L,et al. Proline metabo-lism and transport in plant development[J]. Amino Acids, 2010,39:949-962

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Analysis on Differences of Protein in Stylar Canal During Early Pollination Associated with Self-incompatible Prunus domestica × armeniaca ‘Fengweimeigui’

1. Paulownia R&D Center of China, State Forestry Administration, Zhengzhou 450003, Henan, China

2. Non-timber Forestry R&D Center, Chinese Academy of Forestry, Zhengzhou 450003, Henan, China

Received Date: 2014-01-06

Abstract: This study focused on the differences of protein in stylar canal between that from self-incompatible Prunus domestica × armeniaca ‘Fengweimeigui’ and that from cross-pollination (ACP) (‘Fengweimeigui’ × ‘Konglongdan’). Two dimension difference gel electrophoresis (2D-DIGE) and mass spectrum (MS) detection techniques were used in this study. The results showed that there were 79 different protein spots in stylar canal between that from self-pollination and that from cross-pollination, and there was a huge difference, more than double, in terms of protein expression among 43 protein spots, including 6 up-expressed and 37 down-expressed in self-pollination stylar canal compared with that in cross-pollination stylar canal. Only 34 protein spots were identified unambiguously through mass spectrometry analysis, including 5 kinds which were catalytic protein, defence/stress protein, and structural protein, respectively.

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Reference (43)

[1]	李芳东,杜红岩,杨绍彬,等. ‘风味玫瑰'—极早熟杏李杂交新品种[J].中国果树,2007,(3):91-96.
[2]	?27] 杨绍彬,李芳东,杜红岩,等.杏李种间杂交品种适宜授粉组合筛选试验[J].中国果树,2008(5):28-30.
[3]	李芳东,杜红岩,杨绍彬,等.杏李种间杂交极早熟新品种风味玫瑰[J].中国果树,2007,(3):5-7.
[4]	王熙龙,曹振强.风味玫瑰杏李早期丰产栽培技术[J]. 北方果树,2005(4):29.
[5]	李小远,马玉磊,刘阳,等.小报春不同授粉组合亲和性研究[J].西北植物学报,2012,32(12):2426-2431.
[6]	雷家军,吴超.卷丹与亚洲百合种间杂交花粉管生长的荧光观察[J].东北农业大学学报,2012,43(1):117-120.
[7]	罗春香,缑红星,盛瑞芳,等.杏李杂交种风味玫瑰引种表现及主要栽培技术[J].山西果树,2011,1:14-15.
[8]	陈超,李保国,齐国辉,等.果树自交不亲和机理及应用研究进展. 河北林果研究,2005,20(1):86-90.
[9]	吴燕,陈学森,冯建荣,等. 杏杂种一代群体S 基因的遗传研究. 园艺学报,2005,32(4):397-402.
[10]	Silva N F, Goring D R. Mechanisms of self-incompatibility in flowering plants[J]. Cellular and Molecular Life Sciences,2001,58: 1988-2007.
[11]	Foster E, Levesque-Lemay M, Schneiderman D,et al. Characterization of a gene highly expressed in the Brassica napus pistil that encodes a novel proline-rich protein[J]. Sex Plant Reprod, 2005,17:261-267.
[12]	Wang X, Zafian P, Choudhary M,et al. The PR5K receptor protein kinase from Arabidopsis thaliana is structurally related to a family of plant defense proteins[J]. Proceedings of the National Academy of Sciences of the USA, 1996, 93:2598-2602.
[13]	Furuyama T, Dzelzkalns V A. A novel calcium-binding protein is expressed in brassica pistils and anthers late in flower development[J]. Plant Molecular Biology,1999,39:729-737.
[14]	Fukai E, Nishio T, Nasrallah M. Molecular genetic analysis of the candidate gene for MOD, a locus required for self-incompatibility in Brassica rapa[J]. Molecular Genetics and Genomics,2001,265:519-525.
[15]	Sassa H, Hirano H. Style-specific and developmentally regulated accumulation of a glycosylated thaumatin/PR5-like protein in Japanese pear (Pyrus serotina Rehd.)[J]. Planta,1998,205:514-521.
[16]	De Graaf B H J, Knuiman B A, Derksen J, et al. Characterization and localization of the transmitting tissue-specific PELPIII proteins of Nocotiana tabacum[J]. Journal of Experimental Botany, 2003, 54(380):55-63.
[17]	De Graaf B H J, Knuiman B A, van Der Weerden G M, et al. The PELPIII glycoprotein's in Solanaceae : stylar expression and transfer into pollen tube walls[J]. Sex Plant Reprod,2004,16:245-252.
[18]	Expression of an isoflavone reductase-like gene enhanced by pollen tube growth in pistils of Solanum tuberosum[J]. Plant Molecular Biology,1997,33:923-929.
[19]	O'Brien M, Bertrand C, Matton D P. Characterization of a fertilization-induced and developmentally regulated plasma- membrane aquaporin expressed in reproductive tissue, in the wild potato Solanum chacoense Bitt[J]. Planta,2002,215: 485-493.
[20]	Kuboyama T. A novel thaumatin-like protein gene of tobacco is specifically expressed in the transmitting tissue of stigma and style[J]. Sex Plant Reprod,1998,11:251-256.
[21]	Luu D T, Marty-Mazars D, Trick M, et al. Pollen-stigma adhesion in Brassica spp involves SLG and SLR1 glycoproteins[J]. Plant Cell, 1999,11(2):251-262.
[22]	Doughty J, Dixon S, Hiscock S J, et al. PCP-A1, a defensin-like Brassica pollen coat protein that binds the S locus glycoprotein, is the product of gametophytic gene expression[J]. Plant Cell, 1998, 10(8):1333-1347.
[23]	Samuel M A, Chong Y T, Haasen K E, et al. Cellular pathways regulating responses to compatible and self-incompatible pollen in Brassica and Arabidopsis stigmas intersect at Exo70A1, a putative component of the exocyst complex[J]. Plant Cell,2009,21 (9):2655-2671.
[24]	Dai S, Chen T, Chong K, et al. Proteomics identification of differentially expressed associated with pollen germination and tube growth reveals characteristics of germinated Oryza sativa pollen[J]. Mol Cell Proteomics,2007,6 (2):207-230.
[25]	Sheoran I S, Pederson E J, Ross A R, et al. Dynamics of protein expression during pollen germination in canola(Brassica napus)[J]. Planta, 2009,230 (4):779-793.
[26]	Wang Z, Wan g Z, Shi L,et al. Proteomic alterations of Brassica napus root in response to boron deficiency[J]. Plant Mol Biol,2010, 74(3):265-278.
[27]	Yao Y, Sun H, Xu F, et al. Comparative proteome analysis of metabolic changes by low phosphorus stress in two Brassica napus genotypes[J]. Planta, 2011, 233(3):523-537.
[28]	郑洲,陈学森,冯宝春,等.杏品种授粉生物学研究[J].果树学报,2004,21(6):327-331.
[29]	张绍铃,房经贵,杨记磙.果树自交不亲和性的遗传与生理机制及其研究[J].果树学报,2001,18(1):49-52.
[30]	Pieton JM, Steer MW. A model for the mechanism of tip extension in pollen tubes [J]. Journal of Theoretical Biology,1982, 98: 15-20.
[31]	Pierson ES. Rhodamine-phalloidin staining of F-actin in pollen after dimethysulphoxide permeabilization[J]. Sex Plant Reproduction, 1988,1:83-87.
[32]	Lazzaro M D micrdilaments. The spormatogenous body cell of the conifer Picea abies contains actin Protoplasma[J]. 1998, 201:194-201.
[33]	张鹏.杨树杂交不亲和性的细胞学及生理生化基础.北京:北京林业大学,2013.
[34]	Brewbaker J L, Kwack B H. The essential role of calcium ion in pollen germination and pollen tube growth[J]. Am J Bot, 1963, 50 (9): 859-865.
[35]	Rathore K S, Cork R J, Robinson K R. A cytoplasmic gradient of Ca²⁺ is correlated with the growth of lily pollen tubes[J]. Dev Biol, 1991, (148):612-619.
[36]	Gu Y.Q, Vernoud V, Fu Y, et al. ROPgTPase regulation of pollen tubegrowth through the dynamics of tip-localized F-actin[J]. J. Exp. Bot,2003,(54):93-10.
[37]	尚桂军. TteRRF, AmCBL1初步晶体学和嗜热菌ATCC27502-ATP合酶晶体生长研究.北京:北京林业大学. 2010.
[38]	Okuda S, Tsutsui H, Shiina K, et al. Defensin-like polypeptide LUREs are pollen tube attractants secreted from synergid cells[J]. nature, 458 (7236):357-361.
[39]	Amien S, Kliwer I, Márton M L, et al. Defensin-like ZmES 4 mediates pollen tube burst in maizevia opening of the potassium channel KZM1[J]. PLoS Biol, 2010, 8 (6):e1000388.
[40]	Kessler S A, Shimosato-Asano H, Keinath N F, et al. Conserved molecular components for pollen tube reception and fungal invasion[J]. Science, 2010, 330:968-971.
[41]	张水军,曾千春,卢秀萍,等.植物富含甘氨酸蛋白的研究进展[J].中国农学通报,2010,26(4):54-58.
[42]	孙培亮,王志亮,李金明,等.热休克蛋白70研究新进展[J].中国兽医学报,2007,27(2):284-288.
[43]	Lehmann S, Funck D, Szabados L,et al. Proline metabo-lism and transport in plant development[J]. Amino Acids, 2010,39:949-962

Analysis on Differences of Protein in Stylar Canal During Early Pollination Associated with Self-incompatible Prunus domestica × armeniaca ‘Fengweimeigui’

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通讯作者: 陈斌, bchen63@163.com

Proportional views

Analysis on Differences of Protein in Stylar Canal During Early Pollination Associated with Self-incompatible Prunus domestica × armeniaca ‘Fengweimeigui’

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