Pollen Viability and Preservation of Dendrobium lindleyi

DENG Xi-mei; ZHENG Bao-qiang; GUO Xin; WANG Yan

The pollen of Dendrobium lindleyi was employed as experimental material to investigate the determination method of pollen viability and pollen storage. The results showed that: (1) The optimum culture medium was 100 g·L-1 sucrose + 100 mg·L-1 H3BO3 + 80 mg·L-1 CaCl2. TTC staining was not adapted to detect pollen viability of Den. lindleyi. (2) The pollen germinating rate was over 65% during 0~8 days after blooming. As pollen parents, the male receptivity was over 85% during this period, which showed a better result than bud stage and droop stage. (3) Different storage methods had different effects on pollen viability of Den. lindleyi. The viability of pollen stored at dry condition in 4 ℃ declined to 0 after 80 days. However, after stored at humid condition in 4 ℃, freeze-wetting and freeze-drying in -20 ℃ for 90 days, the pollen viabilities were about 1.4%, 21.7%, and 6.4% respectively. As pollen parents, the pollen freeze-wetting stored in -20 ℃ for 90 days could still set seeds normally, while the setting rate was obviously decreased when using other storage methods.

1. Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China

Received Date: 2012-12-31

Abstract: The pollen of Dendrobium lindleyi was employed as experimental material to investigate the determination method of pollen viability and pollen storage. The results showed that: (1) The optimum culture medium was 100 g·L-1 sucrose + 100 mg·L-1 H3BO3 + 80 mg·L-1 CaCl2. TTC staining was not adapted to detect pollen viability of Den. lindleyi. (2) The pollen germinating rate was over 65% during 0~8 days after blooming. As pollen parents, the male receptivity was over 85% during this period, which showed a better result than bud stage and droop stage. (3) Different storage methods had different effects on pollen viability of Den. lindleyi. The viability of pollen stored at dry condition in 4 ℃ declined to 0 after 80 days. However, after stored at humid condition in 4 ℃, freeze-wetting and freeze-drying in -20 ℃ for 90 days, the pollen viabilities were about 1.4%, 21.7%, and 6.4% respectively. As pollen parents, the pollen freeze-wetting stored in -20 ℃ for 90 days could still set seeds normally, while the setting rate was obviously decreased when using other storage methods.

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[1]	王雁, 李振坚, 彭红明. 石斛兰资源生产应用[M]. 北京:中国林业出版社, 2007:11.
[2]	吉占和, 陈心启, 罗毅波,等. 中国植物志:第19卷[M]. 北京: 科学出版社, 2006: 78.
[3]	Pacini E.Orchid pollen dispersal units and reproductive consequences[C]//Tiiu K, Arditti J, Sek M W. Orchid biology: reviews and perspectives X. Springer-Verlag New York Inc, 2009:185-194.
[4]	郑宝强, 王雁, 彭镇华,等.杂种卡特兰花粉萌发和花粉贮藏性研究[J]. 热带亚热带植物学报, 2012,20 (1):13-18.
[5]	郭丽霞, 莫饶. 海南原生墨兰花粉萌发试验初报[J]. 热带农业科学, 2007,23 (6) :594-597.
[6]	胡适宜, 杨坚. 春兰花粉壁的结构及其与花粉粘合的关系[J]. 植物学报, 1989,31(6):414-421.
[7]	Pritchard H W, Prendergast F G. Factors influencing the germination and storage characteristics of orchid pollen [M]//Pritchard H W. Modern Methods in Orchid Conservation: The Role of Physiology, Ecology and Management. Cambridge: Cambridge University Press, 1989: 1-16.
[8]	律春燕, 王雁, 朱向涛,等.黄牡丹花粉生活力测定方法的比较研究[J].林业科学研究, 2010,23 (2):272-277.
[9]	朱惜晨. 芍药花粉生活力测定与杂交亲本选择初步研究[J]. 福建林业科技, 2007, 34(2):121-123.
[10]	李枝林, 王玉英, 王卜琼. 兰花远缘杂交育种技术研究[J].中国野生植物资源, 2007, 26 (4) : 53-56.
[11]	李振坚, 亢秀萍, 王雁. 重唇石斛传粉生物学与显微研究动态[J].西北植物学报, 2009,29 (9):1804-1810.
[12]	吴应祥. 中国兰花[M].北京:中国林业出版社, 1993: 106-107.
[13]	欧阳英. 几种兰科花卉的离体保存技术研究[D]. 北京:北京林业大学,2010.

Pollen Viability and Preservation of Dendrobium lindleyi

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