Properties Analysis of Lucilia sericata Larva Fat

YUAN Dong-qiang; HE Zhao; SUN Long; ZHAO Min; FENG Ying

Volume 27 Issue 6

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Properties Analysis of Lucilia sericata Larva Fat

1.
Research Institute of Resources Insects, Chinese Academy of Forestry, Key Laboratory of Cultivation and Utilization of Resources Insects, State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2014-06-15

Abstract

The fatty composition and thermodynamic property of Lucilia sericata larva fat were studied. The fat was extracted by four methods: solvent extraction, ultrasonic wave-microwave assisted extraction, soxhlet extraction and enzyme-assisted aqueous extraction. The fatty acids composition and thermodynamic property of the fat were analyzed by GC-MS and DSC respectively. 15 kinds of fatty acid were detected in the fat by solvent extraction, and 17 kinds of fatty acid were detected by the other three methods. Among them, oleic acid, linoleic acid, palmitic acid and palmitoleic acid were the major compositions, accounted for about 89% of total fatty acid. The results of quantitative analysis showed that the contents of oleic acid, palmitic acid and palmitoleic acid were 248.47 mg·g-1, 243.51 mg·g-1, and 174.96 mg·g-1 respectively. Unsaturated fatty acid ratio was higher, accounts for about 65% of the fatty acid content and there were also a little amount of linoleic acid, arachidonic acid and DHA. The thermodynamic characters of fats extracted by different methods were distinctive. The melting point and glass transition temperature were also distinctive. The results showed that the fat compositions of solvent extraction was the worst, the oil quality of ultrasonic wave-microwave assisted extraction was the best. The ultrasonic wave-microwave was the appropriate extraction method of the insect fat which has the value of development and utilization.
- Lucilia sericata Meigen,
- fatty acid,
- oil,
- qualitative,
- GC-MS,
- DSC

References

[1]	Burtis C A, Ashwood E R, Aldrich J E. Tietz fundamentals of clinical chemistry[M]. WB Saunders company, 1996.
[2]	Gibney M J, Vorster H H, Kok F J. Introduction to human nutrition[J]. The human nutrition textbook series (1), 2002.
[3]	Voet D, Voet J G. Biochemistry. Hoboken[J]. John Wiley & Sons, 2004,1:591.
[4]	Mattson F H, Grundy S M. Comparison of effects of dietary saturated, monounsaturated, and polyunsaturated fatty acids on plasma lipids and lipoproteins in man.[J]. Journal of lipid research, 1985,26(2):194-202.
[5]	Goodnight S H, Harris W S, Connor W E, et al. Polyunsaturated fatty acids, hyperlipidemia, and thrombosis.[J]. Arteriosclerosis, Thrombosis, and Vascular Biology, 1982,2(2):87-113.
[6]	王华志, 王道波, 李秋霖, 等. 油脂中脂肪酸成分与人体健康[J]. 粮油加工, 2010(06):16-19.
[7]	何钊, 冯颖, 孙龙, 等. 黄粉虫多糖响应面法提取及抗氧化活性[J]. 食品与生物技术学报, 2011(05):641-647.
[8]	翁梁, 温鲁, 杨芳, 等. 不同提取方法对蛹虫草多糖抗氧化性的影响[J]. 食品科技, 2008(11):180-182.
[9]	Kerridge A, Lappin Scott H, Stevens J R. Antibacterial properties of larval secretions of the blowfly, Lucilia sericata[J]. Medical and Veterinary Entomology, 2005,19(3):333-337.
[10]	Casteels P, Ampe C, Jacobs F, et al. Apidaecins: antibacterial peptides from honeybees.[J]. The EMBO journal, 1989,8(8):2387.
[11]	陈淑媛. 蝇蛆油脂化学成分、理化特性及其对动物实验性烫伤治疗药效的研究[D]. 华中农业大学, 2007.
[12]	廉振民, 李文宾, 刘万霞, 等. 中国昆虫油脂的开发利用及研究现状[J]. 延安大学学报:自然科学版, 2008(01):59-63.
[13]	Van Huis A, Van Itterbeeck J, Klunder H, et al. Edible insects: future prospects for food and feed security[J]. FAO Forestry Paper (FAO), 2013.
[14]	王纲荣, 周克明, 陈红兵, 等. 南昌口岸蝇、蚊类的本底调查报告[J]. 中国国境卫生检疫杂志, 2001(05):287-289.
[15]	余小辉, 涂小云. 丝光绿蝇研究概况[J]. 中国媒介生物学及控制杂志, 2010(04).
[16]	张海生, 陈锦屏. 蜂蛹脂肪超声波提取工艺研究及脂肪酸成分分析[J]. 天然产物研究与开发, 2007(02):299-302.
[17]	段爱莉, 高贵田, 潘静. 气相色谱-质谱联用法对蚕蛹油中脂肪酸成分分析[J]. 蚕桑通报, 2011(01):14-16.
[18]	林春梅, 周鸣谦. 不同方法提取蚕蛹油的GC-MS分析[J]. 中国粮油学报, 2013(01):112-116.
[19]	李福伟, 王文亮, 李恩霞, 等. GC-MS法测定黄粉虫脂肪酸组成及含量的研究[J]. 食品研究与开发, 2008(10):92-94.
[20]	蒙松年, 肖小芹, 汪世平, 等. 美洲大蠊中脂溶性化学成分的GC-MS研究[J]. 中南药学, 2008(01):23-25.
[21]	郭澄, 魏道智. 超临界萃取蝼蛄脂肪酸成分及其气相色谱-质谱分析[J]. 分析化学, 2006(S1):15-18.
[22]	张旭, 董晓萍, 邓斌贝, 等. GC-MS分析蜣螂油脂的化学成分[J]. 华西药学杂志, 2006(03):247-248.
[23]	刘可春, 侯海荣, 韩利文, 等. 黄粉虫脂肪油中脂肪酸的GC-MS分析[J]. 中国食物与营养, 2008(10):30-31.
[24]	Lands W E. Long-term fat intake and biomarkers.[J]. The American journal of clinical nutrition, 1995,61(3):721S-725S.
[25]	Okada T, Furuhashi N, Kuromori Y, et al. Plasma palmitoleic acid content and obesity in children[J]. The American journal of clinical nutrition, 2005,82(4):747-750.
[26]	McGarry J D, Dobbins R L. Fatty acids, lipotoxicity and insulin secretion[J]. Diabetologia, 1999,42(2):128-138.
[27]	Maedler K, Spinas G A, Dyntar D, et al. Distinct effects of saturated and monounsaturated fatty acids on β-cell turnover and function[J]. Diabetes, 2001,50(1):69-76.
[28]	Lichtenstein A H, Appel L J, Brands M, et al. Diet and lifestyle recommendations revision 2006 A scientific statement from the American Heart Association nutrition committee[J]. Circulation, 2006,114(1):82-96.
[29]	Alonso A, Martínez-González M á. Olive oil consumption and reduced incidence of hypertension: the SUN study[J]. Lipids, 2004,39(12):1233-1238.
[30]	Perona J S, Vögler O, Sánchez-Domínguez J M, et al. Consumption of virgin olive oil influences membrane lipid composition and regulates intracellular signaling in elderly adults with type 2 diabetes mellitus[J]. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 2007,62(3):256-263.
[31]	Terés S, Barcelo-Coblijn G, Benet M, et al. Oleic acid content is responsible for the reduction in blood pressure induced by olive oil[J]. Proceedings of the National Academy of Sciences, 2008,105(37):13811-13816.
[32]	Wongtangtintharn S, Oku H, Iwasaki H, et al. Effect of branched-chain fatty acids on fatty acid biosynthesis of human breast cancer cells.[J]. Journal of nutritional science and vitaminology, 2004,50(2):137-143.
[33]	Wolk A, Vessby B, Ljung H, et al. Evaluation of a biological marker of dairy fat intake.[J]. The American journal of clinical nutrition, 1998,68(2):291-295.
[34]	Smedman A E, Gustafsson I, Berglund L G, et al. Pentadecanoic acid in serum as a marker for intake of milk fat: relations between intake of milk fat and metabolic risk factors[J]. The American journal of clinical nutrition, 1999,69(1):22-29.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Properties Analysis of Lucilia sericata Larva Fat

1. Research Institute of Resources Insects, Chinese Academy of Forestry, Key Laboratory of Cultivation and Utilization of Resources Insects, State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2014-06-15

Abstract: The fatty composition and thermodynamic property of Lucilia sericata larva fat were studied. The fat was extracted by four methods: solvent extraction, ultrasonic wave-microwave assisted extraction, soxhlet extraction and enzyme-assisted aqueous extraction. The fatty acids composition and thermodynamic property of the fat were analyzed by GC-MS and DSC respectively. 15 kinds of fatty acid were detected in the fat by solvent extraction, and 17 kinds of fatty acid were detected by the other three methods. Among them, oleic acid, linoleic acid, palmitic acid and palmitoleic acid were the major compositions, accounted for about 89% of total fatty acid. The results of quantitative analysis showed that the contents of oleic acid, palmitic acid and palmitoleic acid were 248.47 mg·g-1, 243.51 mg·g-1, and 174.96 mg·g-1 respectively. Unsaturated fatty acid ratio was higher, accounts for about 65% of the fatty acid content and there were also a little amount of linoleic acid, arachidonic acid and DHA. The thermodynamic characters of fats extracted by different methods were distinctive. The melting point and glass transition temperature were also distinctive. The results showed that the fat compositions of solvent extraction was the worst, the oil quality of ultrasonic wave-microwave assisted extraction was the best. The ultrasonic wave-microwave was the appropriate extraction method of the insect fat which has the value of development and utilization.

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Reference (34)

[1]	Burtis C A, Ashwood E R, Aldrich J E. Tietz fundamentals of clinical chemistry[M]. WB Saunders company, 1996.
[2]	Gibney M J, Vorster H H, Kok F J. Introduction to human nutrition[J]. The human nutrition textbook series (1), 2002.
[3]	Voet D, Voet J G. Biochemistry. Hoboken[J]. John Wiley & Sons, 2004,1:591.
[4]	Mattson F H, Grundy S M. Comparison of effects of dietary saturated, monounsaturated, and polyunsaturated fatty acids on plasma lipids and lipoproteins in man.[J]. Journal of lipid research, 1985,26(2):194-202.
[5]	Goodnight S H, Harris W S, Connor W E, et al. Polyunsaturated fatty acids, hyperlipidemia, and thrombosis.[J]. Arteriosclerosis, Thrombosis, and Vascular Biology, 1982,2(2):87-113.
[6]	王华志, 王道波, 李秋霖, 等. 油脂中脂肪酸成分与人体健康[J]. 粮油加工, 2010(06):16-19.
[7]	何钊, 冯颖, 孙龙, 等. 黄粉虫多糖响应面法提取及抗氧化活性[J]. 食品与生物技术学报, 2011(05):641-647.
[8]	翁梁, 温鲁, 杨芳, 等. 不同提取方法对蛹虫草多糖抗氧化性的影响[J]. 食品科技, 2008(11):180-182.
[9]	Kerridge A, Lappin Scott H, Stevens J R. Antibacterial properties of larval secretions of the blowfly, Lucilia sericata[J]. Medical and Veterinary Entomology, 2005,19(3):333-337.
[10]	Casteels P, Ampe C, Jacobs F, et al. Apidaecins: antibacterial peptides from honeybees.[J]. The EMBO journal, 1989,8(8):2387.
[11]	陈淑媛. 蝇蛆油脂化学成分、理化特性及其对动物实验性烫伤治疗药效的研究[D]. 华中农业大学, 2007.
[12]	廉振民, 李文宾, 刘万霞, 等. 中国昆虫油脂的开发利用及研究现状[J]. 延安大学学报:自然科学版, 2008(01):59-63.
[13]	Van Huis A, Van Itterbeeck J, Klunder H, et al. Edible insects: future prospects for food and feed security[J]. FAO Forestry Paper (FAO), 2013.
[14]	王纲荣, 周克明, 陈红兵, 等. 南昌口岸蝇、蚊类的本底调查报告[J]. 中国国境卫生检疫杂志, 2001(05):287-289.
[15]	余小辉, 涂小云. 丝光绿蝇研究概况[J]. 中国媒介生物学及控制杂志, 2010(04).
[16]	张海生, 陈锦屏. 蜂蛹脂肪超声波提取工艺研究及脂肪酸成分分析[J]. 天然产物研究与开发, 2007(02):299-302.
[17]	段爱莉, 高贵田, 潘静. 气相色谱-质谱联用法对蚕蛹油中脂肪酸成分分析[J]. 蚕桑通报, 2011(01):14-16.
[18]	林春梅, 周鸣谦. 不同方法提取蚕蛹油的GC-MS分析[J]. 中国粮油学报, 2013(01):112-116.
[19]	李福伟, 王文亮, 李恩霞, 等. GC-MS法测定黄粉虫脂肪酸组成及含量的研究[J]. 食品研究与开发, 2008(10):92-94.
[20]	蒙松年, 肖小芹, 汪世平, 等. 美洲大蠊中脂溶性化学成分的GC-MS研究[J]. 中南药学, 2008(01):23-25.
[21]	郭澄, 魏道智. 超临界萃取蝼蛄脂肪酸成分及其气相色谱-质谱分析[J]. 分析化学, 2006(S1):15-18.
[22]	张旭, 董晓萍, 邓斌贝, 等. GC-MS分析蜣螂油脂的化学成分[J]. 华西药学杂志, 2006(03):247-248.
[23]	刘可春, 侯海荣, 韩利文, 等. 黄粉虫脂肪油中脂肪酸的GC-MS分析[J]. 中国食物与营养, 2008(10):30-31.
[24]	Lands W E. Long-term fat intake and biomarkers.[J]. The American journal of clinical nutrition, 1995,61(3):721S-725S.
[25]	Okada T, Furuhashi N, Kuromori Y, et al. Plasma palmitoleic acid content and obesity in children[J]. The American journal of clinical nutrition, 2005,82(4):747-750.
[26]	McGarry J D, Dobbins R L. Fatty acids, lipotoxicity and insulin secretion[J]. Diabetologia, 1999,42(2):128-138.
[27]	Maedler K, Spinas G A, Dyntar D, et al. Distinct effects of saturated and monounsaturated fatty acids on β-cell turnover and function[J]. Diabetes, 2001,50(1):69-76.
[28]	Lichtenstein A H, Appel L J, Brands M, et al. Diet and lifestyle recommendations revision 2006 A scientific statement from the American Heart Association nutrition committee[J]. Circulation, 2006,114(1):82-96.
[29]	Alonso A, Martínez-González M á. Olive oil consumption and reduced incidence of hypertension: the SUN study[J]. Lipids, 2004,39(12):1233-1238.
[30]	Perona J S, Vögler O, Sánchez-Domínguez J M, et al. Consumption of virgin olive oil influences membrane lipid composition and regulates intracellular signaling in elderly adults with type 2 diabetes mellitus[J]. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 2007,62(3):256-263.
[31]	Terés S, Barcelo-Coblijn G, Benet M, et al. Oleic acid content is responsible for the reduction in blood pressure induced by olive oil[J]. Proceedings of the National Academy of Sciences, 2008,105(37):13811-13816.
[32]	Wongtangtintharn S, Oku H, Iwasaki H, et al. Effect of branched-chain fatty acids on fatty acid biosynthesis of human breast cancer cells.[J]. Journal of nutritional science and vitaminology, 2004,50(2):137-143.
[33]	Wolk A, Vessby B, Ljung H, et al. Evaluation of a biological marker of dairy fat intake.[J]. The American journal of clinical nutrition, 1998,68(2):291-295.
[34]	Smedman A E, Gustafsson I, Berglund L G, et al. Pentadecanoic acid in serum as a marker for intake of milk fat: relations between intake of milk fat and metabolic risk factors[J]. The American journal of clinical nutrition, 1999,69(1):22-29.

Properties Analysis of Lucilia sericata Larva Fat

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通讯作者: 陈斌, bchen63@163.com

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Properties Analysis of Lucilia sericata Larva Fat

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