SRAP Analysis on the Effect of Geographic Isolation on Population Genetic Structure of Populus davidiana in Tibetan-inhabited Regions in Southwest China

HE Cheng-zhong; LI Jia-man; YUN Tao; ZONG Dan; ZHOU An-pei; OU Guang-long; YIN Wu-yuan

Volume 28 Issue 2

Article Contents

Turn off MathJax

Article Navigation > Forest Research > 2015 > 28(2): 152-157

Citation:

SRAP Analysis on the Effect of Geographic Isolation on Population Genetic Structure of Populus davidiana in Tibetan-inhabited Regions in Southwest China

1.
Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, Yunnan, China
2.
Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming 650224, Yunnan, China
3.
Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, Yunnan, China

Received Date: 2014-09-20

Abstract

130 individuals of 9 Populus davidiana populations collected from 3 Tibetan-inhabited regions in southwest China were examined by sequence-related amplified polymorphism (SRAP) markers to determine the genetic structure. 99 polymorphic loci were obtained from the 9 populations using 7 selected primers, and its percentage was 59.28%. As analyzed by POPGENE soft, the average percentage of polymorphic loci (PPB=33.80%), Nei's genetic diversity (H=0.130 9) and Shannon's information index (I=0.213 7) indicated that P. davidiana distributed in Tibetan-inhabited regions in Southwest China held a lower level of genetic diversity than that of P. davidiana distributed in Northeast China. The coefficient gene differentiation (Gst) was 0.325 5, indicating more variation existed in the individuals of the populations. A weak correlation was found between geographic distance and genetic distance (r=0.349, P=94.5%), which indicated that the mountain barriers strongly influenced genetic differentiation among populations. Unweighted pair-group method with arithmetical averages cluster analyses (UPGMA) revealed that the Weixi population from Diqing region had grouped with the four populations from Ganzi region, while the Deqin and Xianggelila population from Diqing region had grouped with the two populations from Changdu region. According to the analysis on genetic structure of P. davidiana populations, it is proposed that natural population should be protected in situ, and the germplasm resources should be constructed to enhence the gene flow.
- Populus davidiana,
- SRAP markers,
- geographical isolation,
- genetic variation,
- genetic structure

References

[1]	郭树平, 李春明. 中国山杨资源与发展现状[J]. 林业科技, 2012, 37(1): 48-52.
[2]	Zhang X, Wu N, Li C. Physiological and growth responses of Populus davidiana ecotypes to different soil water contents[J]. J Arid Environments, 2005, 60: 567-579.
[3]	Lee K M, Kim Y Y, Hyun J O. Genetic variation in populations of Populus davidiana Dode based on microsatellite marker analysis[J]. Genes and Genomics, 2011, 33: 163-171.
[4]	张金然, 尚洁, 王秋玉. 山杨杂种无性系的SSR分子标记遗传多样性[J]. 植物研究, 2006, 26(4): 447-451, 460.
[5]	白卉. 山杨遗传多样性研究与核心种质构建及利用[D]. 东北林业大学, 2010.
[6]	Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica[J]. Theor Appl Genet, 2001, 103: 455-461.
[7]	王从彦. SRAP标记在植物遗传多样性中的应用进展[J]. 生物技术, 2011, 21(5): 87-90.
[8]	Bharti A, Neelam R, Yadav V C, et al. Sequence-related amplified polymorphism (SRAP) molecular marker system and its applications in crop improvement[J]. Mol breading, 2012, 30: 1635-1648.
[9]	Robarts D W H, Wolfe A D. Sequence-related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology[J]. Applications in Plant Sciences, 2014, 2(7): 1400017.
[10]	李秀, 徐坤, 巩彪. 生姜种质遗传多样性和亲缘关系的SRAP分析[J]. 中国农业科学, 2014, 47(4): 718-726.
[11]	Babaei S, Talebi M, Masoud B, et al. Analysis of genetic diversity among saffron (Crocus sativus) accessions from different regions of Iran as revealed by SRAP markers[J]. Scientia Horticulturae, 2014, 171: 27-31.
[12]	Peng L, Ru M, Wang B Q, et al. Genetic diversity assessment of a germplasm collection of Salvia miltiorrhiza Bunge. based on morphology, ISSR and SRAP markers[J]. Biochemical Systematics and Ecology, 2014, 55: 84-92.
[13]	尹春英, 彭幼红, 罗建勋, 等. 杨属遗传多样性研究进展[J]. 植物生态学报, 2004, 28(5): 711-722.
[14]	葛颂, 洪德元. 生物多样性研究的原理方法[M]. 北京: 中国科学技术出版社, 1994.
[15]	Leberg P L. Genetic considerations in the design of introduction programs transactions of the north American wildlife[J]. Natural Resource Conference, 1990, 55: 609-619.
[16]	文亚峰, 韩文军, 吴顺. 植物遗传多样性及其影响因素[J]. 中南林业科技大学学报, 2010, 30(12): 80-86.
[17]	Hamriek J L, Godt M J W.Effects of life history traits on genetic diversity in plant species[J]. Philosophical Transactions of London Biological Sciences, 1996, 351: 1291-1298.
[18]	Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals[J]. Genetics, 1978, 89(3): 583-590.
[19]	Hamrick J L. Isozymes and the analysis of genetic structure in plant populations. In: Solitis D E et al (Eds.), Isoxymes in plant biology[M]. London: Chapman and Hall, 1990.
[20]	Grant V. The Evolutionary process: a critical study of evolutionary theory[M]. New York: Columbia University Press, 1991.
[21]	Serbner K T, Evans J E, Morreale S J. et al. Genetic divergence among populations of the yellow-bellied slider turtle (Pseudemys scripta) separated by aquatic and terrestrial habitats[J]. Copeia, 1986, 3: 691-700.
[22]	Starkin M. Gene flow and geographics structure of natural populations[J]. Science, 1987, 236: 787-792.
[23]	Manel S. Landscape genetics: combining landscape ecology and population genetics[J]. Trends in Ecology and Evolution, 2003, 18(4): 189-197.
[24]	Wright S. Evolution in mendelian population[J]. Genetics, 1931, 16: 91-96.
[25]	Slatkin M.Isolation by distance in equilibrium and non-equilibrium populations[J]. Evolution, 1993, 47: 264-279.
[26]	林立, 李纪元, 倪穗, 等. 岛屿地理隔离对山茶种群遗传结构的影响[J]. 林业科学研究, 2012, 25(3): 378-384.
[27]	Oromi N, Boix A R, Sanuy D, et al. Genetic variability in geographic populations of the natterjack toad (Bufo calamita)[J]. Ecology and Evolution, 2012, 2(8): 2018-2026.
[28]	Wang L J. Examining the full effects of landscape heterogeneity of spatial genetic variation: a multiple matrix regression approach for quantifying geographic and ecological isolation[J]. Evolution, 2013, 67(12): 3403-3411.
[29]	Orsini L, Vanoverbeke J, Swillen I, et al. Drivers of population genetic differentiation in the wild: isolation by dspersal limitation, isolation by adaptation and isolation by colonization[J]. Molecular Ecology, 2013, 22: 5983-5999.
[30]	余树全, 刘军, 付达荣, 等. 川西高原青阳派基因资源特点[J]. 浙江林学院学报, 2003, 20(1): 27-31.
[31]	刘友全, 付达荣. 川西高原青杨组基因资源及开发利用[J]. 中南林学院学报, 2004, 24(5): 129-131.
[32]	万雪琴, 张帆, 钟宇, 等. 中国西南地区乡土杨树基因资源的保护与利用[J]. 林业科学, 2009, 45(4): 139-144.

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Get Citation

PDF

XML

Article views(3505) PDF downloads(1235) Cited by()

Proportional views

SRAP Analysis on the Effect of Geographic Isolation on Population Genetic Structure of Populus davidiana in Tibetan-inhabited Regions in Southwest China

1. Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Southwest Forestry University, Kunming 650224, Yunnan, China

2. Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming 650224, Yunnan, China

3. Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, Yunnan, China

Received Date: 2014-09-20

Abstract: 130 individuals of 9 Populus davidiana populations collected from 3 Tibetan-inhabited regions in southwest China were examined by sequence-related amplified polymorphism (SRAP) markers to determine the genetic structure. 99 polymorphic loci were obtained from the 9 populations using 7 selected primers, and its percentage was 59.28%. As analyzed by POPGENE soft, the average percentage of polymorphic loci (PPB=33.80%), Nei's genetic diversity (H=0.130 9) and Shannon's information index (I=0.213 7) indicated that P. davidiana distributed in Tibetan-inhabited regions in Southwest China held a lower level of genetic diversity than that of P. davidiana distributed in Northeast China. The coefficient gene differentiation (Gst) was 0.325 5, indicating more variation existed in the individuals of the populations. A weak correlation was found between geographic distance and genetic distance (r=0.349, P=94.5%), which indicated that the mountain barriers strongly influenced genetic differentiation among populations. Unweighted pair-group method with arithmetical averages cluster analyses (UPGMA) revealed that the Weixi population from Diqing region had grouped with the four populations from Ganzi region, while the Deqin and Xianggelila population from Diqing region had grouped with the two populations from Changdu region. According to the analysis on genetic structure of P. davidiana populations, it is proposed that natural population should be protected in situ, and the germplasm resources should be constructed to enhence the gene flow.

HTML

Reference (32)

[1]	郭树平, 李春明. 中国山杨资源与发展现状[J]. 林业科技, 2012, 37(1): 48-52.
[2]	Zhang X, Wu N, Li C. Physiological and growth responses of Populus davidiana ecotypes to different soil water contents[J]. J Arid Environments, 2005, 60: 567-579.
[3]	Lee K M, Kim Y Y, Hyun J O. Genetic variation in populations of Populus davidiana Dode based on microsatellite marker analysis[J]. Genes and Genomics, 2011, 33: 163-171.
[4]	张金然, 尚洁, 王秋玉. 山杨杂种无性系的SSR分子标记遗传多样性[J]. 植物研究, 2006, 26(4): 447-451, 460.
[5]	白卉. 山杨遗传多样性研究与核心种质构建及利用[D]. 东北林业大学, 2010.
[6]	Li G, Quiros C F. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica[J]. Theor Appl Genet, 2001, 103: 455-461.
[7]	王从彦. SRAP标记在植物遗传多样性中的应用进展[J]. 生物技术, 2011, 21(5): 87-90.
[8]	Bharti A, Neelam R, Yadav V C, et al. Sequence-related amplified polymorphism (SRAP) molecular marker system and its applications in crop improvement[J]. Mol breading, 2012, 30: 1635-1648.
[9]	Robarts D W H, Wolfe A D. Sequence-related amplified polymorphism (SRAP) markers: A potential resource for studies in plant molecular biology[J]. Applications in Plant Sciences, 2014, 2(7): 1400017.
[10]	李秀, 徐坤, 巩彪. 生姜种质遗传多样性和亲缘关系的SRAP分析[J]. 中国农业科学, 2014, 47(4): 718-726.
[11]	Babaei S, Talebi M, Masoud B, et al. Analysis of genetic diversity among saffron (Crocus sativus) accessions from different regions of Iran as revealed by SRAP markers[J]. Scientia Horticulturae, 2014, 171: 27-31.
[12]	Peng L, Ru M, Wang B Q, et al. Genetic diversity assessment of a germplasm collection of Salvia miltiorrhiza Bunge. based on morphology, ISSR and SRAP markers[J]. Biochemical Systematics and Ecology, 2014, 55: 84-92.
[13]	尹春英, 彭幼红, 罗建勋, 等. 杨属遗传多样性研究进展[J]. 植物生态学报, 2004, 28(5): 711-722.
[14]	葛颂, 洪德元. 生物多样性研究的原理方法[M]. 北京: 中国科学技术出版社, 1994.
[15]	Leberg P L. Genetic considerations in the design of introduction programs transactions of the north American wildlife[J]. Natural Resource Conference, 1990, 55: 609-619.
[16]	文亚峰, 韩文军, 吴顺. 植物遗传多样性及其影响因素[J]. 中南林业科技大学学报, 2010, 30(12): 80-86.
[17]	Hamriek J L, Godt M J W.Effects of life history traits on genetic diversity in plant species[J]. Philosophical Transactions of London Biological Sciences, 1996, 351: 1291-1298.
[18]	Nei M. Estimation of average heterozygosity and genetic distance from a small number of individuals[J]. Genetics, 1978, 89(3): 583-590.
[19]	Hamrick J L. Isozymes and the analysis of genetic structure in plant populations. In: Solitis D E et al (Eds.), Isoxymes in plant biology[M]. London: Chapman and Hall, 1990.
[20]	Grant V. The Evolutionary process: a critical study of evolutionary theory[M]. New York: Columbia University Press, 1991.
[21]	Serbner K T, Evans J E, Morreale S J. et al. Genetic divergence among populations of the yellow-bellied slider turtle (Pseudemys scripta) separated by aquatic and terrestrial habitats[J]. Copeia, 1986, 3: 691-700.
[22]	Starkin M. Gene flow and geographics structure of natural populations[J]. Science, 1987, 236: 787-792.
[23]	Manel S. Landscape genetics: combining landscape ecology and population genetics[J]. Trends in Ecology and Evolution, 2003, 18(4): 189-197.
[24]	Wright S. Evolution in mendelian population[J]. Genetics, 1931, 16: 91-96.
[25]	Slatkin M.Isolation by distance in equilibrium and non-equilibrium populations[J]. Evolution, 1993, 47: 264-279.
[26]	林立, 李纪元, 倪穗, 等. 岛屿地理隔离对山茶种群遗传结构的影响[J]. 林业科学研究, 2012, 25(3): 378-384.
[27]	Oromi N, Boix A R, Sanuy D, et al. Genetic variability in geographic populations of the natterjack toad (Bufo calamita)[J]. Ecology and Evolution, 2012, 2(8): 2018-2026.
[28]	Wang L J. Examining the full effects of landscape heterogeneity of spatial genetic variation: a multiple matrix regression approach for quantifying geographic and ecological isolation[J]. Evolution, 2013, 67(12): 3403-3411.
[29]	Orsini L, Vanoverbeke J, Swillen I, et al. Drivers of population genetic differentiation in the wild: isolation by dspersal limitation, isolation by adaptation and isolation by colonization[J]. Molecular Ecology, 2013, 22: 5983-5999.
[30]	余树全, 刘军, 付达荣, 等. 川西高原青阳派基因资源特点[J]. 浙江林学院学报, 2003, 20(1): 27-31.
[31]	刘友全, 付达荣. 川西高原青杨组基因资源及开发利用[J]. 中南林学院学报, 2004, 24(5): 129-131.
[32]	万雪琴, 张帆, 钟宇, 等. 中国西南地区乡土杨树基因资源的保护与利用[J]. 林业科学, 2009, 45(4): 139-144.

SRAP Analysis on the Effect of Geographic Isolation on Population Genetic Structure of Populus davidiana in Tibetan-inhabited Regions in Southwest China

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views

SRAP Analysis on the Effect of Geographic Isolation on Population Genetic Structure of Populus davidiana in Tibetan-inhabited Regions in Southwest China

HTML

Catalog

Export File

Citation

Format

Content