Growth and Physiological Response of Nitraria tangutorum to Salt Stress

NI Jian-wei; YANG Xiu-yan; ZHANG Hua-xin; WU Hai-wen; XU Xiu-yu; LIU Tao

Volume 28 Issue 2

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Growth and Physiological Response of Nitraria tangutorum to Salt Stress

1.
Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
2.
Research Center of Saline and Alkali Land of State Forestry Administration, Beijing 100091, China
3.
Guangdong Academy of Forestry, Guangzhou 510520, Guangdong, China

Received Date: 2014-06-30

Abstract

Taking Nitraria tangutorum suspension cells as material and treated by different salt concentrations (0, 100, 150, 200 and 250 mmol·L-1 NaCl), the growth characteristics of cells and the dynamic changes and correlations of physiological indexes with time, including soluble protein, soluble sugar, proline, peroxidase (POD), superoxide dismutase (SOD) and malondialdehyde (MDA), were analyzed. The results are as follows. (1)Salt treatment could significantly affect the growth of suspension cells, the cells growth conform to the Logistic growth curve model and the growth of suspension cells treated by 100 mmol·L-1 NaCl was the fastest, and with the salt concentration increasing the growth were inhibited. (2)Salt treatment could significantly affect the physiological indexes, and at the early stages of culture all the indexes increased with the salt concentration increasing. While this trend decreased gradually with the extending of incubation time and the Proline, SOD, MDA increased in initial stage and then decreased with the salt concentration increasing in the later period of culture. Under same salt concentration, the soluble sugar got to the maximum at first in the osmotic adjustment index and the SOD got to the maximum in initial stage in the antioxidant enzymes index. And they are the first line of defensing the salt stress in osmotic adjustment and antioxidant enzymes respectively. (3)The fresh weight of suspension cells was significant and fearfully significant negatively correlated with the contents of soluble sugar and MDA. All the physiological indexes showed a significantly positive correlation. It indicated that the balance of cells internal physiological metabolic processes was regulated by various substances synergies under the salt environment.
- Nitraria tangutorum,
- halophytes,
- suspended cells,
- physiological index,
- salt stress,
- salt-tolerance mechanisms

References

[2] Navrot N, Rouhier N, Gelhaye E, et al. Reactive oxygen species generation and antioxidant systems in plant mitochondria[J]. Physiologia Plantarum,2007, 129(1): 185-195.
[2]	Parida A K, Das A B. Salt tolerance and salinity effects on plants: a review[J]. Ecotoxicology and Environmental Safety,2005, 60(3): 324-349.
[3] 廖岩,彭友贵,陈桂珠. 植物耐盐性机理研究进展[J]. 生态学报,2007, 27(5): 2077-2089.
[4] 李彦,张英鹏,孙明,等. 盐分胁迫对植物的影响及植物耐盐机理研究进展[J]. 中国农学通报, 2008, 24(1): 258-265.
[5] 杨秀艳,张华新,张丽,等. NaCl胁迫对唐古特白刺幼苗生长及离子吸收、运输与分配的影响[J]. 林业科学,2013, 49(9): 165-171.
[6] Adams P, Thomas J C, Vernon D M, et al. Distinct cellular and organismic responses to salt stress[J]. Plant and Cell Physiology,1992, 33(8): 1215-1223.
[7] Banu M N A, Hoque M A, Watanabe-Sugimoto M, et al. Proline and glycinebetaine induce antioxidant defense gene expression and suppress cell death in cultured tobacco cells under salt stress[J]. Journal of Plant Physiology,2009, 166(2): 146-156.
[8] 陈秉初,苏维埃. 苜蓿悬浮细胞对盐胁迫的反应和适应[J]. 实验生物学报,1995, 28(4): 349-354.
[9] 赵素然,梁宇. 毛白杨愈伤组织悬浮细胞耐盐性研究[J]. 广西植物,2000, 20(4): 351-355.
[10]	房江育,王贺,张福锁. 硅对盐胁迫烟草悬浮细胞的影响[J]. 作物学报, 2003, 29(4): 610-614.
[11]	周荣仁,邰根福,杨燮荣. 烟草悬浮培养细胞耐盐性的研究[J]. 植物生理学通讯, 1984(5): 13-16.
[12]	刘璞,孟令军,张会霞,等. cAMP参与烟草悬浮细胞的ABA信号转导(英文)[J]. Acta Botanica Sinica, 2002, 44(12): 1432-1437.
[13]	Duval I, Brochu V, Simard M, et al. Thaxtomin A induces programmed cell death in Arabidopsis thaliana suspension-cultured cells[J]. Planta,2005, 222(5): 820-831.
[14]	Kim J K, Bamba T, Harada K, et al. Time-course metabolic profiling in Arabidopsis thaliana cell cultures after salt stress treatment[J]. Journal of Experimental Botany,2007, 58(3): 415-424.
[15]	Chen J, Cheng T, Wang P, et al. Salinity-induced changes in protein expression in the halophytic plant Nitraria sphaerocarpa[J]. Journal of Proteomics,2012, 75(17): 5226-5243.
[16]	马启慧. 耐盐碱植物白刺的开发与利用[J]. 黑龙江农业科学,2007(5): 116-117.
[17]	王尚德,康向阳. 唐古特白刺研究现状与建议[J]. 植物遗传资源学报,2005(2): 231-235.
[18]	李红,章英才,张鹏. 白刺属植物研究综述[J]. 农业科学研究,2006(4): 61-64.
[19]	王文,蒋文兰,谢忠奎,等. NaCl胁迫对唐古特白刺幼苗生理指标的影响[J]. 草地学报,2012, 20(5): 907-913.
[20]	Yang Y, Shi R, Wei X, et al. Effect of salinity on antioxidant enzymes in calli of the halophyte Nitraria tangutorum Bobr[J]. Plant Cell, Tissue and Organ Culture,2010, 102(3): 387-395.
[21]	崔永忠,廖声熙,崔凯,等. 贵州山区山苍子苗年生长规律[J]. 林业科学研究,2013, 26(4): 501-505.
[22]	刘萍,李明军. 植物生理学实验技术[M]. 北京: 科学出版社, 2007.
[23]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[24]	宋纯鹏,王学路. 植物生理学[M]. 北京: 科学出版社, 2009.
[25]	Hoque A, Okuma E, Banu N A, et al. Exogenous proline mitigates the detrimental effects of salt stress more than exogenous betaine by increasing antioxidant enzyme activities[J]. Journal of Plant Physiology,2007, 164(5): 553-561.
[26]	Shalata A, Tal M. The effect of salt stress on lipid peroxidation and antioxidants in the leaf of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii[J]. Physiologia Plantarum,1998, 104(2): 169-174.
[27]	倪建伟,武香,张华新,等. 3 种白刺耐盐性的对比分析[J]. 林业科学研究,2012, 25(1): 48-53.
[28]	赵可夫. 盐生植物及其对盐渍生境的适应生理[M]. 北京:科学出版社, 2005.
[29]	孙志宾,齐兴云,张洪涛,等. NaCl 对拟南芥 (Arabidopsis thaliana)生长与渗透调节的影响[J]. 山东科学,2006, 19(3): 7-14.
[30]	Hoque A, Okuma E, Banu N A, et al. Exogenous proline mitigates the detrimental effects of salt stress more than exogenous betaine by increasing antioxidant enzyme activities[J]. Journal of Plant Physiology,2007, 164(5): 553-561.
[31]	王宝山,姚敦义. 盐胁迫对沙枣愈伤组织膜透性, 膜脂过氧化和 SOD 活性的影响[J]. 河北农业大学学报,1993, 16(3): 20-24.
[32]	沈静,杨青川,曹致中,等. 低温胁迫对野牛草细胞膜和保护酶活性的影响[J]. 中国草地学报,2010(2): 98-102.
[33]	Hernández J A, Ferrer M A, Jiménez A, et al. Antioxidant Systems and O₂·^-/H₂O₂ Production in the Apoplast of Pea Leaves. Its Relation with Salt-Induced Necrotic Lesions in Minor Veins[J]. Plant Physiology,2001, 127(3): 817-831.
[34]	Wang C, Xu C, Wei J, et al. Enhanced tonoplast H⁺-ATPase activity and superoxide dismutase activity in the halophyte Suaeda salsa containing high level of betacyanin[J]. Journal of Plant Growth Regulation,2008, 27(1): 58-67.
[35]	Tang D, Shi S, Li D, et al. Physiological and biochemical responses of Scytonema javanicum (cyanobacterium) to salt stress[J]. Journal of Arid Environments,2007, 71(3): 312-320.
[36]	Chaparzadeh N, D’Amico M L, Khavari-Nejad R, et al. Antioxidative responses of Calendula officinalis under salinity conditions[J]. Plant Physiology and Biochemistry,2004, 42(9): 695-701.
[37]	武香,倪建伟,张华新,等. 盐胁迫对 3 种白刺渗透调节物质的影响[J]. 东北林业大学学报, 2012, 40(1): 44-47.
[38]	杨升,刘正祥,张华新,等. 3个树种苗期耐盐性综合评价及指标筛选[J]. 林业科学,2013, 49(1): 91-98.
[39]	Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry,1976, 72(1): 248-254.
[40]	代莉慧,蔡禄,张鲁刚,等. NaCl 和 Na₂CO₃ 胁迫对盐爪爪种子萌发过程中生理生化变化的研究[J]. 种子,2012, 30(11): 53-55.
[41]	Xiong L, Zhu J K. Molecular and genetic aspects of plant responses to osmotic stress[J]. Plant, Cell and Environment,2002, 25(2): 131-139.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Growth and Physiological Response of Nitraria tangutorum to Salt Stress

1. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

2. Research Center of Saline and Alkali Land of State Forestry Administration, Beijing 100091, China

3. Guangdong Academy of Forestry, Guangzhou 510520, Guangdong, China

Received Date: 2014-06-30

Abstract: Taking Nitraria tangutorum suspension cells as material and treated by different salt concentrations (0, 100, 150, 200 and 250 mmol·L-1 NaCl), the growth characteristics of cells and the dynamic changes and correlations of physiological indexes with time, including soluble protein, soluble sugar, proline, peroxidase (POD), superoxide dismutase (SOD) and malondialdehyde (MDA), were analyzed. The results are as follows. (1)Salt treatment could significantly affect the growth of suspension cells, the cells growth conform to the Logistic growth curve model and the growth of suspension cells treated by 100 mmol·L-1 NaCl was the fastest, and with the salt concentration increasing the growth were inhibited. (2)Salt treatment could significantly affect the physiological indexes, and at the early stages of culture all the indexes increased with the salt concentration increasing. While this trend decreased gradually with the extending of incubation time and the Proline, SOD, MDA increased in initial stage and then decreased with the salt concentration increasing in the later period of culture. Under same salt concentration, the soluble sugar got to the maximum at first in the osmotic adjustment index and the SOD got to the maximum in initial stage in the antioxidant enzymes index. And they are the first line of defensing the salt stress in osmotic adjustment and antioxidant enzymes respectively. (3)The fresh weight of suspension cells was significant and fearfully significant negatively correlated with the contents of soluble sugar and MDA. All the physiological indexes showed a significantly positive correlation. It indicated that the balance of cells internal physiological metabolic processes was regulated by various substances synergies under the salt environment.

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Reference (41)

[1]	[2] Navrot N, Rouhier N, Gelhaye E, et al. Reactive oxygen species generation and antioxidant systems in plant mitochondria[J]. Physiologia Plantarum,2007, 129(1): 185-195.
[2]	Parida A K, Das A B. Salt tolerance and salinity effects on plants: a review[J]. Ecotoxicology and Environmental Safety,2005, 60(3): 324-349.
[3]	[3] 廖岩,彭友贵,陈桂珠. 植物耐盐性机理研究进展[J]. 生态学报,2007, 27(5): 2077-2089.
[4]	[4] 李彦,张英鹏,孙明,等. 盐分胁迫对植物的影响及植物耐盐机理研究进展[J]. 中国农学通报, 2008, 24(1): 258-265.
[5]	[5] 杨秀艳,张华新,张丽,等. NaCl胁迫对唐古特白刺幼苗生长及离子吸收、运输与分配的影响[J]. 林业科学,2013, 49(9): 165-171.
[6]	[6] Adams P, Thomas J C, Vernon D M, et al. Distinct cellular and organismic responses to salt stress[J]. Plant and Cell Physiology,1992, 33(8): 1215-1223.
[7]	[7] Banu M N A, Hoque M A, Watanabe-Sugimoto M, et al. Proline and glycinebetaine induce antioxidant defense gene expression and suppress cell death in cultured tobacco cells under salt stress[J]. Journal of Plant Physiology,2009, 166(2): 146-156.
[8]	[8] 陈秉初,苏维埃. 苜蓿悬浮细胞对盐胁迫的反应和适应[J]. 实验生物学报,1995, 28(4): 349-354.
[9]	[9] 赵素然,梁宇. 毛白杨愈伤组织悬浮细胞耐盐性研究[J]. 广西植物,2000, 20(4): 351-355.
[10]	房江育,王贺,张福锁. 硅对盐胁迫烟草悬浮细胞的影响[J]. 作物学报, 2003, 29(4): 610-614.
[11]	周荣仁,邰根福,杨燮荣. 烟草悬浮培养细胞耐盐性的研究[J]. 植物生理学通讯, 1984(5): 13-16.
[12]	刘璞,孟令军,张会霞,等. cAMP参与烟草悬浮细胞的ABA信号转导(英文)[J]. Acta Botanica Sinica, 2002, 44(12): 1432-1437.
[13]	Duval I, Brochu V, Simard M, et al. Thaxtomin A induces programmed cell death in Arabidopsis thaliana suspension-cultured cells[J]. Planta,2005, 222(5): 820-831.
[14]	Kim J K, Bamba T, Harada K, et al. Time-course metabolic profiling in Arabidopsis thaliana cell cultures after salt stress treatment[J]. Journal of Experimental Botany,2007, 58(3): 415-424.
[15]	Chen J, Cheng T, Wang P, et al. Salinity-induced changes in protein expression in the halophytic plant Nitraria sphaerocarpa[J]. Journal of Proteomics,2012, 75(17): 5226-5243.
[16]	马启慧. 耐盐碱植物白刺的开发与利用[J]. 黑龙江农业科学,2007(5): 116-117.
[17]	王尚德,康向阳. 唐古特白刺研究现状与建议[J]. 植物遗传资源学报,2005(2): 231-235.
[18]	李红,章英才,张鹏. 白刺属植物研究综述[J]. 农业科学研究,2006(4): 61-64.
[19]	王文,蒋文兰,谢忠奎,等. NaCl胁迫对唐古特白刺幼苗生理指标的影响[J]. 草地学报,2012, 20(5): 907-913.
[20]	Yang Y, Shi R, Wei X, et al. Effect of salinity on antioxidant enzymes in calli of the halophyte Nitraria tangutorum Bobr[J]. Plant Cell, Tissue and Organ Culture,2010, 102(3): 387-395.
[21]	崔永忠,廖声熙,崔凯,等. 贵州山区山苍子苗年生长规律[J]. 林业科学研究,2013, 26(4): 501-505.
[22]	刘萍,李明军. 植物生理学实验技术[M]. 北京: 科学出版社, 2007.
[23]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[24]	宋纯鹏,王学路. 植物生理学[M]. 北京: 科学出版社, 2009.
[25]	Hoque A, Okuma E, Banu N A, et al. Exogenous proline mitigates the detrimental effects of salt stress more than exogenous betaine by increasing antioxidant enzyme activities[J]. Journal of Plant Physiology,2007, 164(5): 553-561.
[26]	Shalata A, Tal M. The effect of salt stress on lipid peroxidation and antioxidants in the leaf of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii[J]. Physiologia Plantarum,1998, 104(2): 169-174.
[27]	倪建伟,武香,张华新,等. 3 种白刺耐盐性的对比分析[J]. 林业科学研究,2012, 25(1): 48-53.
[28]	赵可夫. 盐生植物及其对盐渍生境的适应生理[M]. 北京:科学出版社, 2005.
[29]	孙志宾,齐兴云,张洪涛,等. NaCl 对拟南芥 (Arabidopsis thaliana)生长与渗透调节的影响[J]. 山东科学,2006, 19(3): 7-14.
[30]	Hoque A, Okuma E, Banu N A, et al. Exogenous proline mitigates the detrimental effects of salt stress more than exogenous betaine by increasing antioxidant enzyme activities[J]. Journal of Plant Physiology,2007, 164(5): 553-561.
[31]	王宝山,姚敦义. 盐胁迫对沙枣愈伤组织膜透性, 膜脂过氧化和 SOD 活性的影响[J]. 河北农业大学学报,1993, 16(3): 20-24.
[32]	沈静,杨青川,曹致中,等. 低温胁迫对野牛草细胞膜和保护酶活性的影响[J]. 中国草地学报,2010(2): 98-102.
[33]	Hernández J A, Ferrer M A, Jiménez A, et al. Antioxidant Systems and O₂·^-/H₂O₂ Production in the Apoplast of Pea Leaves. Its Relation with Salt-Induced Necrotic Lesions in Minor Veins[J]. Plant Physiology,2001, 127(3): 817-831.
[34]	Wang C, Xu C, Wei J, et al. Enhanced tonoplast H⁺-ATPase activity and superoxide dismutase activity in the halophyte Suaeda salsa containing high level of betacyanin[J]. Journal of Plant Growth Regulation,2008, 27(1): 58-67.
[35]	Tang D, Shi S, Li D, et al. Physiological and biochemical responses of Scytonema javanicum (cyanobacterium) to salt stress[J]. Journal of Arid Environments,2007, 71(3): 312-320.
[36]	Chaparzadeh N, D’Amico M L, Khavari-Nejad R, et al. Antioxidative responses of Calendula officinalis under salinity conditions[J]. Plant Physiology and Biochemistry,2004, 42(9): 695-701.
[37]	武香,倪建伟,张华新,等. 盐胁迫对 3 种白刺渗透调节物质的影响[J]. 东北林业大学学报, 2012, 40(1): 44-47.
[38]	杨升,刘正祥,张华新,等. 3个树种苗期耐盐性综合评价及指标筛选[J]. 林业科学,2013, 49(1): 91-98.
[39]	Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry,1976, 72(1): 248-254.
[40]	代莉慧,蔡禄,张鲁刚,等. NaCl 和 Na₂CO₃ 胁迫对盐爪爪种子萌发过程中生理生化变化的研究[J]. 种子,2012, 30(11): 53-55.
[41]	Xiong L, Zhu J K. Molecular and genetic aspects of plant responses to osmotic stress[J]. Plant, Cell and Environment,2002, 25(2): 131-139.

Growth and Physiological Response of Nitraria tangutorum to Salt Stress

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通讯作者: 陈斌, bchen63@163.com

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Growth and Physiological Response of Nitraria tangutorum to Salt Stress

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