The Structural and Phylogenetic Analysis of Late Expression Factor 8

LUO Ji; ZHOU Guo-ying; ZHU Ji-yu

Volume 28 Issue 2

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The Structural and Phylogenetic Analysis of Late Expression Factor 8

1.
College of Forestry, Central South University of Forestry and Technology, Changsha 410004, Hu'nan, China
2.
2.Guangxi Zhuang Autonomous Region Forestry Research Institute, Nanning 530002, Guangxi, China

Received Date: 2014-04-23

Abstract

Late expression factor 8 (lef-8) gene is an early expression gene, and codes the largest subunit of RNA polymerase in baculovirus. The authors sequenced the lef-8 gene of Buzura suppressaria nuclear polyhedrosis virus (BsNPV). It had 2 634 bp coding region and encoding 877 amino acid residues. Some signal sequences were found in untranslated region (UTR): possible promoter and transcription initiation site were found in 5'-UTR, and a putative polyadenylation signal sequence was found in 3'-UTR. But whether they had substantial function needs further research. The protein sequence of lef-8 gene in BsNPV was low homologous to model NPVs. But they had nearly the same signal sequence. By phylogenetic analysis of lef-8 gene in baculovirus, it was found that the nuclear polyhedrosis virus (NPV) and the granulosis virus (GV) was separated obviously to two branches, while the NPV broke into 4 branches. And evolutionary relationship in baculovirus was largely associated with its host.
- Buzura suppressaria,
- nuclear polyhedrosis virus,
- baculovirus,
- lef-8 gene,
- gene structure,
- phylogenetic analysis

References

[1]	梁光祥, 何国业, 蒙庆卓. 油桐尺蠖危害桉树用灭幼脲Ⅲ号防治试验初报[J]. 广西林业科学, 1998, 27(3): 41-44.
[2]	陈川, 张永安, 王玉珠, 等. 油桐尺蠖核型多角体病毒(BusuNPV)生物学与检测技术研究[J]. 林业科学研究, 2011, 24(3): 410-414.
[3]	祝庆荃, 刘宇兰, 刘军, 等. 油桐尺蠖核型多角体病毒对实验动物的毒性试[J]. 中国茶叶, 1982, (4): 30-32.
[4]	谢天恩, 彭辉银, 葛云垣, 等. 大尺蠖核型多角体病毒的生产及其应用[J]. 中国茶叶, 1980, (5): 18-20.
[5]	陈明树, 孙富林, 许大海. 油桐尺蠖核型多角体病毒生物学特性的研究[J]. 湖北农业科学, 1981, (12): 26-28.
[6]	彭辉银, 陈新文, 金锋, 等. 保存时间对油桐尺蠖核型多角体病毒杀虫剂毒力的影响[J]. 生物防治通报, 1993, 9(3): 119-121.
[7]	曾云添, 汤显春, 刘宇蓝, 等. 油桐尺蠖核型多角体病毒毒力的生物测定[J]. 广东茶叶科技, 1983, (4): 1-5.
[8]	孙松柏, 陈明树, 孙富林, 等. 油桐尺蠖核型多角体病毒DNA的限制性内切酶分析[J]. 华中农学院学报, 1985, 4(2): 75-80.
[9]	刘明富, 谢天恩. 原位化学切割法对油桐尺蠖核型多角体病毒两种相似多肽的比较研究[J]. 病毒学杂志, 1991, 6(1): 75-78.
[10]	梁布锋. 油桐尺蠖核型多角体病毒杀虫剂的血清学检测[J]. 湖北农业科学, 1990, (10): 20,28.
[11]	Acharya A, Gopinathan K. Characterization of late gene expression factors lef-9 and lef-8 from Bombyx mori nucleopolyhedrovirus[J]. Journal of General Virology, 2002, 83(8): 2015-2023.
[12]	Crouch E A, Cox L T, Morales K G, et al. Inter-subunit interactions of the Autographa californica M nucleopolyhedrovirus RNA polymerase[J]. Virology, 2007, 367(2): 265-274.
[13]	Jose J, Jalali S K, Shivalingaswamy T M, et al. Molecular Characterization of Nucleopolyhedrovirus of Three Lepidopteran Pests Using Late Expression Factor-8 Gene[J]. Indian Journal of Virology, 2013, 24(1): 59-65.
[14]	Blissard G W, Kogan P H, Wei R, et al. A synthetic early promoter from a baculovirus: Roles of the TATA box and conserved start site CAGT sequence in basal levels of transcription[J]. Virology, 1992, 190(2): 783-793.
[15]	Kogan P H, Chen X, Blissard G W. Overlapping TATA-dependent and TATA-independent early promoter activities in the baculovirus gp64 envelope fusion protein gene[J]. Journal of Virology, 1995, 69(3): 1452-1461.
[16]	Xing K, Deng R, Wang J, et al. Analysis and prediction of baculovirus promoter sequences[J]. Virus Research, 2005, 113(1): 64-71.
[17]	Huang H, Wang M, Deng F, et al. The ha72 Core Gene of Baculovirus Is Essential for Budded Virus Production and Occlusion-Derived Virus Embedding, and Amino Acid K22 Plays an Important Role in Its Function[J]. Journal of Virology, 2014, 88(1): 705-709.
[18]	Simón O, Palma L, Beperet I, et al. Sequence comparison between three geographically distinct Spodoptera frugiperda multiple nucleopolyhedrovirus isolates: Detecting positively selected genes[J]. Journal of Invertebrate Pathology, 2011, 107(1): 33-42.
[19]	Tang X D, Xiao Q, Ma X C, et al. Morphology and genome of Euproctis pseudoconspersa nucleopolyhedrovirus[J]. Virus Genes 2009, 38(3): 495-506.
[20]	Ahrens C H, Russell R L Q, Funk C J, et al. The Sequence of the Orgyia pseudotsugata Multinucleocapsid Nuclear Polyhedrosis Virus Genome[J]. Virology, 1997, 229(2): 381-399.
[21]	丁铲. 病毒的进化与传播[J]. 中国家禽, 2010, 32(11): 35-37.
[22]	Zhu Z, Yin F, Liu X, et al. Genome Sequence and Analysis of Buzura suppressaria Nucleopolyhedrovirus: A Group II Alphabaculovirus[J]. Plos One, 2014, 9(1): e86450.
[23]	Thumbi D K, Eveleigh R J M, Lucarotti C J, et al. Complete Sequence, Analysis and Organization of the Orgyia leucostigma Nucleopolyhedrovirus Genome[J]. Viruses, 2011, 3(11): 2301-2327.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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The Structural and Phylogenetic Analysis of Late Expression Factor 8

1. College of Forestry, Central South University of Forestry and Technology, Changsha 410004, Hu'nan, China

2. 2.Guangxi Zhuang Autonomous Region Forestry Research Institute, Nanning 530002, Guangxi, China

Received Date: 2014-04-23

Abstract: Late expression factor 8 (lef-8) gene is an early expression gene, and codes the largest subunit of RNA polymerase in baculovirus. The authors sequenced the lef-8 gene of Buzura suppressaria nuclear polyhedrosis virus (BsNPV). It had 2 634 bp coding region and encoding 877 amino acid residues. Some signal sequences were found in untranslated region (UTR): possible promoter and transcription initiation site were found in 5'-UTR, and a putative polyadenylation signal sequence was found in 3'-UTR. But whether they had substantial function needs further research. The protein sequence of lef-8 gene in BsNPV was low homologous to model NPVs. But they had nearly the same signal sequence. By phylogenetic analysis of lef-8 gene in baculovirus, it was found that the nuclear polyhedrosis virus (NPV) and the granulosis virus (GV) was separated obviously to two branches, while the NPV broke into 4 branches. And evolutionary relationship in baculovirus was largely associated with its host.

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Reference (23)

[1]	梁光祥, 何国业, 蒙庆卓. 油桐尺蠖危害桉树用灭幼脲Ⅲ号防治试验初报[J]. 广西林业科学, 1998, 27(3): 41-44.
[2]	陈川, 张永安, 王玉珠, 等. 油桐尺蠖核型多角体病毒(BusuNPV)生物学与检测技术研究[J]. 林业科学研究, 2011, 24(3): 410-414.
[3]	祝庆荃, 刘宇兰, 刘军, 等. 油桐尺蠖核型多角体病毒对实验动物的毒性试[J]. 中国茶叶, 1982, (4): 30-32.
[4]	谢天恩, 彭辉银, 葛云垣, 等. 大尺蠖核型多角体病毒的生产及其应用[J]. 中国茶叶, 1980, (5): 18-20.
[5]	陈明树, 孙富林, 许大海. 油桐尺蠖核型多角体病毒生物学特性的研究[J]. 湖北农业科学, 1981, (12): 26-28.
[6]	彭辉银, 陈新文, 金锋, 等. 保存时间对油桐尺蠖核型多角体病毒杀虫剂毒力的影响[J]. 生物防治通报, 1993, 9(3): 119-121.
[7]	曾云添, 汤显春, 刘宇蓝, 等. 油桐尺蠖核型多角体病毒毒力的生物测定[J]. 广东茶叶科技, 1983, (4): 1-5.
[8]	孙松柏, 陈明树, 孙富林, 等. 油桐尺蠖核型多角体病毒DNA的限制性内切酶分析[J]. 华中农学院学报, 1985, 4(2): 75-80.
[9]	刘明富, 谢天恩. 原位化学切割法对油桐尺蠖核型多角体病毒两种相似多肽的比较研究[J]. 病毒学杂志, 1991, 6(1): 75-78.
[10]	梁布锋. 油桐尺蠖核型多角体病毒杀虫剂的血清学检测[J]. 湖北农业科学, 1990, (10): 20,28.
[11]	Acharya A, Gopinathan K. Characterization of late gene expression factors lef-9 and lef-8 from Bombyx mori nucleopolyhedrovirus[J]. Journal of General Virology, 2002, 83(8): 2015-2023.
[12]	Crouch E A, Cox L T, Morales K G, et al. Inter-subunit interactions of the Autographa californica M nucleopolyhedrovirus RNA polymerase[J]. Virology, 2007, 367(2): 265-274.
[13]	Jose J, Jalali S K, Shivalingaswamy T M, et al. Molecular Characterization of Nucleopolyhedrovirus of Three Lepidopteran Pests Using Late Expression Factor-8 Gene[J]. Indian Journal of Virology, 2013, 24(1): 59-65.
[14]	Blissard G W, Kogan P H, Wei R, et al. A synthetic early promoter from a baculovirus: Roles of the TATA box and conserved start site CAGT sequence in basal levels of transcription[J]. Virology, 1992, 190(2): 783-793.
[15]	Kogan P H, Chen X, Blissard G W. Overlapping TATA-dependent and TATA-independent early promoter activities in the baculovirus gp64 envelope fusion protein gene[J]. Journal of Virology, 1995, 69(3): 1452-1461.
[16]	Xing K, Deng R, Wang J, et al. Analysis and prediction of baculovirus promoter sequences[J]. Virus Research, 2005, 113(1): 64-71.
[17]	Huang H, Wang M, Deng F, et al. The ha72 Core Gene of Baculovirus Is Essential for Budded Virus Production and Occlusion-Derived Virus Embedding, and Amino Acid K22 Plays an Important Role in Its Function[J]. Journal of Virology, 2014, 88(1): 705-709.
[18]	Simón O, Palma L, Beperet I, et al. Sequence comparison between three geographically distinct Spodoptera frugiperda multiple nucleopolyhedrovirus isolates: Detecting positively selected genes[J]. Journal of Invertebrate Pathology, 2011, 107(1): 33-42.
[19]	Tang X D, Xiao Q, Ma X C, et al. Morphology and genome of Euproctis pseudoconspersa nucleopolyhedrovirus[J]. Virus Genes 2009, 38(3): 495-506.
[20]	Ahrens C H, Russell R L Q, Funk C J, et al. The Sequence of the Orgyia pseudotsugata Multinucleocapsid Nuclear Polyhedrosis Virus Genome[J]. Virology, 1997, 229(2): 381-399.
[21]	丁铲. 病毒的进化与传播[J]. 中国家禽, 2010, 32(11): 35-37.
[22]	Zhu Z, Yin F, Liu X, et al. Genome Sequence and Analysis of Buzura suppressaria Nucleopolyhedrovirus: A Group II Alphabaculovirus[J]. Plos One, 2014, 9(1): e86450.
[23]	Thumbi D K, Eveleigh R J M, Lucarotti C J, et al. Complete Sequence, Analysis and Organization of the Orgyia leucostigma Nucleopolyhedrovirus Genome[J]. Viruses, 2011, 3(11): 2301-2327.

The Structural and Phylogenetic Analysis of Late Expression Factor 8

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通讯作者: 陈斌, bchen63@163.com

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The Structural and Phylogenetic Analysis of Late Expression Factor 8

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