Polyphasic Identification of Two Rhizobium Strains from Woody Legumes in Jianfengling of Hainan Province

CHENG Tao; JIAO Ru-zhen; GAO Jun-lian

Two Rhizobium strains RIF200835 and RIF200845 isolated from woody legumes in Jianfengling National Nature Reserve, Hainan province, were analyzed by molecular methods. Their 16S rDNA sequences showed 98.28% and 98.51% of similality to R. miluonense CCUAU 41251T. respectively. The two strains were also investigated for compositions of fatty acid and quinone. The results showed that they were basically similar to the model strain in content but slightly different in components. The DNA G + C contents of the two strains were 63.68% and 60.56%, falling in the range of 57%65% of the genus of Rhizobia. Their housekeep genes (atpD, glnII, recA and rpoB) formed a clade seperating from the model strain R. miluonense CCUAU 41251T. The results showed that the two strains might be new species of Rhizobium.

1. Research Insititute of Forestry, Chinese Academy of Foresry, Beijing 100091, China

2. Key Laboratory of Forest Cultivation, State Forestry Administration, Beijing 100091, China

3. Research Center of Agricultural Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received Date: 2014-05-14

Abstract: Two Rhizobium strains RIF200835 and RIF200845 isolated from woody legumes in Jianfengling National Nature Reserve, Hainan province, were analyzed by molecular methods. Their 16S rDNA sequences showed 98.28% and 98.51% of similality to R. miluonense CCUAU 41251T. respectively. The two strains were also investigated for compositions of fatty acid and quinone. The results showed that they were basically similar to the model strain in content but slightly different in components. The DNA G + C contents of the two strains were 63.68% and 60.56%, falling in the range of 57%65% of the genus of Rhizobia. Their housekeep genes (atpD, glnII, recA and rpoB) formed a clade seperating from the model strain R. miluonense CCUAU 41251T. The results showed that the two strains might be new species of Rhizobium.

HTML

Reference (15)

[1]	朱剑光, 尉亚辉, 吴异舟. 花生慢生根瘤菌的分离与鉴定[J]. 生物技术, 2006, 16(2):45-48.
[2]	姚志恒. 基因在费氏中华根瘤菌中的验证及对结瘤的影响[J].江西农业学报, 2009, 21(2):8-10, 54.
[3]	FAO(国际粮农组织).豆科植物根瘤菌剂及其利用[M].北京:农业科技出版社, 1988.1921.
[4]	陈文峰, 陈文新.我国豆科植物根瘤菌资源多样性及应用基础研究, 生物学通报, 38(7), 1-3, 2003.
[5]	宁国赞.刘惠琴.马小彤.中国豆科牧草根瘤菌资源的采集保藏及利用[J].草地学报, 1999.7(2);165-172.
[6]	G史晓霞;师尚礼;杨晶;王正凤豆科植物根瘤菌分类研究进展草原与草坪 2006.02.20.
[7]	Terefework Z, Kaijalainen S, Lindstrom K, et al. AFLP fingerprinting as a tool to study the genetic diversity of Rhizobium galegae isolated from Galega orientalis and Galega officinali. Journal of Biotechnology. 2001, 91(23): 169-180.
[8]	Zhu Z L. Utilizing chemical and organic fertilizer reasonable, exp loring friendly fertilizer system [ J ]. Publication of Chinese Academy Science, 2003, 2: 89293.
[9]	彭玉红. 海南尖峰岭木本豆科植物根瘤菌资源研究中国林业科学研究院硕士论文 2009, 05.01.
[10]	Sasser, M. Identification of bacteria by gas chromatography of cellular fatty acids. Newark, DE: MIDI Technical Note . 1990: 101.
[11]	刘天岩. 鸡眼草根瘤菌的多相分类与分子进化研究中国农业大学硕士论文 2013, 05.
[12]	陈坚. 根际 ACC 脱氨酶活性细菌的筛选与多相分类及其对番茄的促生作用研究首都师范大学硕士论文 2012, 05.21.
[13]	Marmur, J. A procedure for isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology. 1961, 3(2): 208-218.
[14]	Johnson, J. L..DNA reassociation and RNA hybridisation of bacteria nucleic acids. Methods in Microbiology. 1995, 18, 33-74.
[15]	Deley, J.. Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. Journal of Bacteriology. 1970, 101(3): 738-754.

Polyphasic Identification of Two Rhizobium Strains from Woody Legumes in Jianfengling of Hainan Province

Abstract

References

Proportional views

通讯作者: 陈斌, bchen63@163.com

Proportional views