Identification and Characterization of a 1-Deoxy-D-xylulose 5-phosphate Synthase Gene From Pinus kesiya var. langbianensis

WANG Yi; ZHOU Xu; BI Wei; YANG Yu-ming; LI Jiang; WANG Juan

Volume 28 Issue 6

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Identification and Characterization of a 1-Deoxy-D-xylulose 5-phosphate Synthase Gene From Pinus kesiya var. langbianensis

1.
The Key Laboratory of Rare and Endangered Forest Plan ts of State Forestry Administration, Yunnan Academy of Forestry, Kunming 650201, China
2.
The Key Laboratory of Foresty Plant Cultivation and Utilization, Yunnan Academy of Forestry, Kunming 650201, Yunnan, China
3.
Southwest Forestry University, Kunming 650224, China

Received Date: 2015-01-19

Abstract

1-deoxy-D-xylulose 5-phosphate synthase(DXS) catalyzes the first and the rate-limiting step of the MEP pathway. In this study, the special primers were designed according to the transcriptome data of Pinus kesiya var. langbianensis(A. Chev.) Gaussen. The full length gene of PkDXS1 was cloned by RT-PCR and RACE, which consisted of 2223 bp open reading frame(ORF) encoding 740 amino acid, the deduced PkDXS1 protein shared 99% and 97% identities with DXS of Pinus densiflora Siebold & Zucc and Picea abies(L.) H. Karst., respectively. Analysis of the deduced amino acid sequence showed that PkDXS1 carried a chloroplast transit peptied, a thiamine diphosphate binding site, and a transketolase motif, which are the important characteristics of DXS enzymes in higher plants. Semiquantity-RT-PCR detection showed that DXS gene expression was up-regulated by wounding treatment.
- Pinus kesiya var. langbianensis(A. Chev.) Gaussen,
- 1-deoxy-d-xylulose 5-phosphate synthase gene,
- cDNA clone,
- gene function analysis

References

[1]	徐明艳,邓桂香,凌万刚.思茅松良种利用方法的探讨[J].种子,2012,31(8):95-101.
[2]	董静曦,张剑,郭辉军.云南与广西产脂松树资源的对比分析[J].林业资源管理,2009,28(3):85-89.
[3]	Le Corre V, Kremer A. The genetic differentiation at quantitative trait loci under local adaptation[J]. Molecular ecology, 2012, 21(7):1548-1566.
[4]	Ribaut J M, de Vicente M C, Delannay X. Molecular breeding in developing countries:challenges and perspectives[J]. Current Opinion in Plant Biology, 2010, 13(2):213-218.
[5]	Zorrilla-Fontanesi Y, Cabeza A, Domínguez P, et al. Quantitative trait loci and underlying candidate genes controlling agronomical and fruit quality traits in octoploid strawberry(Fragaria×ananassa)[J]. Theoretical and applied genetics,2011,123(5):755-778.
[6]	Induri B R, Ellis D R, Slavov G T, et al. Identification of quantitative trait loci and candidate genes for cadmium tolerance in Populus[J]. Tree physiology, 2012,32(5):626-638.
[7]	Mauricio R. Mapping quantitative trait loci in plants:uses and caveats for evolutionary biology[J]. Nature Reviews Genetics,2001,2(5):370-381.
[8]	Salvi S, Tuberosa R. To clone or not to clone plant QTLs:present and future challenges[J]. Trends in plant science, 2005, 10(6):297-304.
[9]	Longhi S, Hamblin M T, Trainotti L, et al. A candidate gene based approach validates Md-PG1 as the main responsible for a QTL impacting fruit texture in apple(Malus x domestica Borkh)[J]. BMC plant biology,2013,13(1):37.
[10]	Wang L, Wang A, Huang X, et al. Mapping quantitative trait loci at high resolution through sequencing-based genotyping of rice recombinant inbred lines[J]. Theoretical and applied genetics,2011,122(2):327-340.
[11]	Guggenheim J A, McMahon G, Kemp J P, et al. A genome-wide association study for corneal curvature identifies the platelet-derived growth factor receptor alpha gene as a quantitative trait locus for eye size in white Europeans[J]. Molecular vision,2013,19:243.
[12]	Rodrigues K C D S, Lima J C, Fett A G. Pine oleoresin:tapping green chemicals, biofuels, food protection, and carbon sequestration from multipurpose trees[J]. Food and Energy Security,2013, 1(2):81-93.
[13]	Tholl D. Terpene synthases and the regulation,diversity and biological roles of terpene metabolism[J]. Current Opinion in Plant Biology,2006,9(1):1-8.
[14]	Vranová E, Coman D, Gruissem W. Network analysis of the MVA and MEP pathways for isoprenoid synthesis[J]. Annual review of plant biology, 2013,64:665-700.
[15]	李思广,付玉嫔,蒋云东.40个高产脂思茅松无性系的松脂化学组成特征[J].西部林业科学,2008,37(2):61-65.
[16]	Xiang S, Usunow G, Lange G, et al. 1-Deoxy-d-Xylulose 5-Phosphate Synthase(DXS), a crucial enzyme for isoprenoids biosynthesis. In isoprenoid synthesis in plants and microorganisms[M]. New York:Springer. 2013, 17-28.
[17]	Walter M H, Floss D S, Paetzold H, et al. Control of plastidial isoprenoid precursor supply:divergent 1-Deoxy-d-Xylulose 5-Phosphate synthase(DXS) isogenes regulate the allocation to primary or secondary metabolism. In isoprenoid synthesis in plants and microorganisms[M]. New York:Springer. 2013, 251-270.
[18]	Enfissi E, Fraser P D, Lois L M, et al. Metabolic engineering of the mevalonate and non-mevalonate isopentenyl diphosphate-forming pathways for the production of health-promoting isoprenoids in tomato[J]. Plant Biotechnology Journal, 2005,3(1):17-27.
[19]	Kim Y B, Kim S M, Kang M K, et al. Regulation of resin acid synthesis in Pinus densiflora by differential transcription of genes encoding multiple 1-deoxy-d-xylulose 5-phosphate synthase and 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase genes[J]. Tree Physiology,2009,29(5):737-749.
[20]	Battilana J, Costantini L, Emanuelli F, et al. The 1-deoxy-d-xylulose 5-phosphate synthase gene co-localizes with a major QTL affecting monoterpene content in grapevine[J]. Theoretical and applied genetics, 2009, 118(4):653-669.
[21]	Xu Y, Liu J, Liang L, et al. Molecular cloning and characterization of three cDNAs encoding 1-deoxy-d-xylulose-5-phosphate synthase in Aquilaria sinensis(Lour.) Gilg[J].Plant Physiology and Biochemistry, 2014,82:133-141.
[22]	Peng G, Wang C, Song S, et al. The role of 1-deoxy-d-xylulose-5-phosphate synthase and phytoene synthase gene family in citrus carotenoid accumulation[J].Plant Physiology and Biochemistry, 2013,71:67-76.
[23]	Phillips M A, Walter M H, Ralph S G, et al. Functional identification and differential expression of 1-deoxy-D-xylulose 5-phosphate synthase in induced terpenoid resin formation of Norway spruce(Picea abies)[J].Plant molecular biology, 2007, 65(3):243-257.
[24]	Azevedo H, Lino-Neto T, Tavares R M. An improved method for high-quality RNA isolation from needles of adult maritime pine trees[J]. Plant molecular biology reporter, 2003,21(4):333-338.
[25]	王雁,李贞,刘小侠,等.白皮松总RNA3种提取方法的比较研究[J].安徽农业科学,2011,39(23):13958-13959.
[26]	Zhang M, Li K, Zhang C, et al. Identification and characterization of class 1 DXS gene encoding 1-deoxy-D-xylulose-5-phosphate synthase, the first committed enzyme of the MEP pathway from soybean[J]. Molecular biology reports, 2009, 36(5):879-887.
[27]	张文勇,刘永刚.思茅松人工林遗传改良研究现状与展望[J].广西林业科学,2010,39(2):93-96.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Identification and Characterization of a 1-Deoxy-D-xylulose 5-phosphate Synthase Gene From Pinus kesiya var. langbianensis

1. The Key Laboratory of Rare and Endangered Forest Plan ts of State Forestry Administration, Yunnan Academy of Forestry, Kunming 650201, China

2. The Key Laboratory of Foresty Plant Cultivation and Utilization, Yunnan Academy of Forestry, Kunming 650201, Yunnan, China

3. Southwest Forestry University, Kunming 650224, China

Received Date: 2015-01-19

Abstract: 1-deoxy-D-xylulose 5-phosphate synthase(DXS) catalyzes the first and the rate-limiting step of the MEP pathway. In this study, the special primers were designed according to the transcriptome data of Pinus kesiya var. langbianensis(A. Chev.) Gaussen. The full length gene of PkDXS1 was cloned by RT-PCR and RACE, which consisted of 2223 bp open reading frame(ORF) encoding 740 amino acid, the deduced PkDXS1 protein shared 99% and 97% identities with DXS of Pinus densiflora Siebold & Zucc and Picea abies(L.) H. Karst., respectively. Analysis of the deduced amino acid sequence showed that PkDXS1 carried a chloroplast transit peptied, a thiamine diphosphate binding site, and a transketolase motif, which are the important characteristics of DXS enzymes in higher plants. Semiquantity-RT-PCR detection showed that DXS gene expression was up-regulated by wounding treatment.

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Reference (27)

[1]	徐明艳,邓桂香,凌万刚.思茅松良种利用方法的探讨[J].种子,2012,31(8):95-101.
[2]	董静曦,张剑,郭辉军.云南与广西产脂松树资源的对比分析[J].林业资源管理,2009,28(3):85-89.
[3]	Le Corre V, Kremer A. The genetic differentiation at quantitative trait loci under local adaptation[J]. Molecular ecology, 2012, 21(7):1548-1566.
[4]	Ribaut J M, de Vicente M C, Delannay X. Molecular breeding in developing countries:challenges and perspectives[J]. Current Opinion in Plant Biology, 2010, 13(2):213-218.
[5]	Zorrilla-Fontanesi Y, Cabeza A, Domínguez P, et al. Quantitative trait loci and underlying candidate genes controlling agronomical and fruit quality traits in octoploid strawberry(Fragaria×ananassa)[J]. Theoretical and applied genetics,2011,123(5):755-778.
[6]	Induri B R, Ellis D R, Slavov G T, et al. Identification of quantitative trait loci and candidate genes for cadmium tolerance in Populus[J]. Tree physiology, 2012,32(5):626-638.
[7]	Mauricio R. Mapping quantitative trait loci in plants:uses and caveats for evolutionary biology[J]. Nature Reviews Genetics,2001,2(5):370-381.
[8]	Salvi S, Tuberosa R. To clone or not to clone plant QTLs:present and future challenges[J]. Trends in plant science, 2005, 10(6):297-304.
[9]	Longhi S, Hamblin M T, Trainotti L, et al. A candidate gene based approach validates Md-PG1 as the main responsible for a QTL impacting fruit texture in apple(Malus x domestica Borkh)[J]. BMC plant biology,2013,13(1):37.
[10]	Wang L, Wang A, Huang X, et al. Mapping quantitative trait loci at high resolution through sequencing-based genotyping of rice recombinant inbred lines[J]. Theoretical and applied genetics,2011,122(2):327-340.
[11]	Guggenheim J A, McMahon G, Kemp J P, et al. A genome-wide association study for corneal curvature identifies the platelet-derived growth factor receptor alpha gene as a quantitative trait locus for eye size in white Europeans[J]. Molecular vision,2013,19:243.
[12]	Rodrigues K C D S, Lima J C, Fett A G. Pine oleoresin:tapping green chemicals, biofuels, food protection, and carbon sequestration from multipurpose trees[J]. Food and Energy Security,2013, 1(2):81-93.
[13]	Tholl D. Terpene synthases and the regulation,diversity and biological roles of terpene metabolism[J]. Current Opinion in Plant Biology,2006,9(1):1-8.
[14]	Vranová E, Coman D, Gruissem W. Network analysis of the MVA and MEP pathways for isoprenoid synthesis[J]. Annual review of plant biology, 2013,64:665-700.
[15]	李思广,付玉嫔,蒋云东.40个高产脂思茅松无性系的松脂化学组成特征[J].西部林业科学,2008,37(2):61-65.
[16]	Xiang S, Usunow G, Lange G, et al. 1-Deoxy-d-Xylulose 5-Phosphate Synthase(DXS), a crucial enzyme for isoprenoids biosynthesis. In isoprenoid synthesis in plants and microorganisms[M]. New York:Springer. 2013, 17-28.
[17]	Walter M H, Floss D S, Paetzold H, et al. Control of plastidial isoprenoid precursor supply:divergent 1-Deoxy-d-Xylulose 5-Phosphate synthase(DXS) isogenes regulate the allocation to primary or secondary metabolism. In isoprenoid synthesis in plants and microorganisms[M]. New York:Springer. 2013, 251-270.
[18]	Enfissi E, Fraser P D, Lois L M, et al. Metabolic engineering of the mevalonate and non-mevalonate isopentenyl diphosphate-forming pathways for the production of health-promoting isoprenoids in tomato[J]. Plant Biotechnology Journal, 2005,3(1):17-27.
[19]	Kim Y B, Kim S M, Kang M K, et al. Regulation of resin acid synthesis in Pinus densiflora by differential transcription of genes encoding multiple 1-deoxy-d-xylulose 5-phosphate synthase and 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase genes[J]. Tree Physiology,2009,29(5):737-749.
[20]	Battilana J, Costantini L, Emanuelli F, et al. The 1-deoxy-d-xylulose 5-phosphate synthase gene co-localizes with a major QTL affecting monoterpene content in grapevine[J]. Theoretical and applied genetics, 2009, 118(4):653-669.
[21]	Xu Y, Liu J, Liang L, et al. Molecular cloning and characterization of three cDNAs encoding 1-deoxy-d-xylulose-5-phosphate synthase in Aquilaria sinensis(Lour.) Gilg[J].Plant Physiology and Biochemistry, 2014,82:133-141.
[22]	Peng G, Wang C, Song S, et al. The role of 1-deoxy-d-xylulose-5-phosphate synthase and phytoene synthase gene family in citrus carotenoid accumulation[J].Plant Physiology and Biochemistry, 2013,71:67-76.
[23]	Phillips M A, Walter M H, Ralph S G, et al. Functional identification and differential expression of 1-deoxy-D-xylulose 5-phosphate synthase in induced terpenoid resin formation of Norway spruce(Picea abies)[J].Plant molecular biology, 2007, 65(3):243-257.
[24]	Azevedo H, Lino-Neto T, Tavares R M. An improved method for high-quality RNA isolation from needles of adult maritime pine trees[J]. Plant molecular biology reporter, 2003,21(4):333-338.
[25]	王雁,李贞,刘小侠,等.白皮松总RNA3种提取方法的比较研究[J].安徽农业科学,2011,39(23):13958-13959.
[26]	Zhang M, Li K, Zhang C, et al. Identification and characterization of class 1 DXS gene encoding 1-deoxy-D-xylulose-5-phosphate synthase, the first committed enzyme of the MEP pathway from soybean[J]. Molecular biology reports, 2009, 36(5):879-887.
[27]	张文勇,刘永刚.思茅松人工林遗传改良研究现状与展望[J].广西林业科学,2010,39(2):93-96.

Identification and Characterization of a 1-Deoxy-D-xylulose 5-phosphate Synthase Gene From Pinus kesiya var. langbianensis

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通讯作者: 陈斌, bchen63@163.com

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Identification and Characterization of a 1-Deoxy-D-xylulose 5-phosphate Synthase Gene From Pinus kesiya var. langbianensis

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