Effect of Ultraviolet Radiation on Cunninghamia lanceolata Somatic Embryogenesis Plantlets

WU Hua; YAN Shan; CHEN Jin-hui; ZHENG Ren-hua; SHI Ji-sen; CHENG Tie-long

Volume 28 Issue 6

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Effect of Ultraviolet Radiation on Cunninghamia lanceolata Somatic Embryogenesis Plantlets

1.
College of Forestry, Nanjing Forestry University, Nanjing 210037, Jiangsu, China
2.
Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 2100037, Jiangsu, China

Received Date: 2015-03-07

Abstract

To study the effect of ultraviolet radiation on Chinese fir(Cunninghamia lanceolata(Lamb.) Hook), the somatic embryogenic plants(emblings) regenerated from Chinese fir were used as testing materials. The emblings were treated by different exposure days under ultraviolet light. The results showed that with the prolonging of exposure time under ultraviolet light, the chlorophyll concentration and chlorophyll fluorescence parameters decreased, while the MDA content increased with the activity of POD and soluble protein. It was revealed that the UV radiation could cause a large amount of MDA in leaf which may damage the cell membranes and further decrease the efficiency of photosynthesis of Chinese fir. Stresses may triggered the emblings responding pathway to protect damages by UV radiation through activating POD competence and soluble protein, but failed to save the plants under sever damages in the investigation, POD may excited the activity soluble protein to protect emblings avoiding from damage caused by ultraviolet radiation, but could not being effectively alleviate.
- Cunninghamia lanceolata,
- UV,
- physiological indicators,
- chlorophyll fluorescence parameters

References

[1]	Mckenzie RL, Aucamp PJ, Bais AF,et al. Changes in biologically-active ultraviolet Radiation reaching the earth's surface[J]. Photochemical and Photobiological Science,2007,6(3):218-231.
[2]	郑有军,刘建飞,王艳娜,等.增强UV-B辐射与其它因子符合作用对植物生长的影响研究[J].西北植物学报,2007,27(8):1702-1712.
[3]	蔡锡安,夏汉平,彭少麟.增强UV-B辐射对植物的影响[J].生态环境,2007,16(3):1044-1052.
[4]	ZHU Peng-jin, YANG Li. Ambient UV-B radiation inhibits the growth and physiology of Brassica napus L. on the Qinghai Tibetan plateau[J].Field crops Research,2015,171:79-85.
[5]	Anclia G, Dobribova, Vassilena Krasteva. Damage and protection of the photosynthetic apparatus from UV-B radiation. I. Effect of ascorbate[J].Journal of plant physiology,2013, 170:251-257.
[6]	GAO Qin, ZHANG Li-Xin. Ultraviolent-B-induced oxidative stress and antioxidant defense systemresponses in ascorbate-deficient vtc1 mutant of Arabidopsis thaliana[J].J plant Physiol.2008,165(2):138-148.
[7]	Zlatko S,Zlatev,Fernando J. C. Plant physiological responses to UV-B radiation[J].Emirates Journal of Food & Agriculture, 2012. 24(6):481-501.
[8]	熊日荣.不同杉木无性系在若干逆境下叶绿素荧光参数的比较分析.福建:福建农林大学,2011.
[9]	曲弈,吴华,施季森,等.体胚再生植株低温胁迫的响应机制[J].林业科技开发,2014(05):49-52.
[10]	陈金慧,周小红,施季森.杉木体细胞胚胎发生及植株再生方法,中国,201010275858.0. 2010.
[11]	高俊风.植物生理学实验指导[M].北京:高等教育出版社,2004:211-221.
[12]	张志良,霍伟菁.植物生理学实验指导[M].北京:高等教育出版社,2004,67-69.
[13]	李合生.植物生理生化实验理论与技术[M].北京:高等教育出版社,2001:216-220.
[14]	路文静,李弈松.植物生理学实验指导[M].北京:中国林业出版社,2012:169-233.
[15]	张莉娜,安黎哲,冯虎元,等.增强UV-B辐射和干旱对春小麦光合作用及其生长的影响[J].西北植物学报,2010,30(5):981-986.
[16]	师生波,尚艳霞,朱鹏锦,等.增补UV-B辐射对高山植物美丽风毛菊光合作用和光合色素的影响[J].草地学报,2010,18(5):607-614.
[17]	陈隆升,陈永忠,彭邵锋,等.油茶对低磷胁迫的生理生化效应研究[J].林业科学研究,2010,23(5):782-786.
[18]	白志英,李存东,赵金锋.干旱胁迫对小麦代换系叶绿素荧光参数的影响及染色体效应初步分析[J].中国农业科学,2011,44(1):47-57.
[19]	褚建民,孟平,张劲松,等.土壤水分胁迫对欧李幼苗光合及叶绿素荧光特性的影响[J].林业科学研究,2008,21(3):295-300.
[20]	SUN Zhi-Yang, JI Kong-Shu. Effects of drought stress on chlorophyll fluorescence of four hybrid tulip clones[J].North-West for University, 2010, 25(4):35-39.
[21]	王兆,刘晓曦,郑国华.低温胁迫对彩叶草光合作用及叶绿素荧光的影响[J].浙江农业学报,2015,27(1):49-56.
[22]	黄亚成,秦云霞.植物中活性氧的研究进展[J].中国农学通报,2012,28(36):219-226.
[23]	孙业民,马兰,李朝周.不同类型酸胁迫对云杉叶细胞膜及其保护系统损伤机制的比较[J].林业科学,2012,48(6):56-61.
[24]	吴建勋,张姗姗.Cr、Co、Pb单一胁迫对浮萍SOD、POD、MDA的影响[J].中国农业通报,2013,29(15):188-194.
[25]	尹永强,胡建斌,邓明军.植物叶片抗氧化系统及其对逆境胁迫的响应研究进展[J].中国农学通报,2007,23(1):105-109.
[26]	Fedinai I, Hidma J, Velitchkova M, et al. UV-B induced stress responses in three rice euhivars[J]. Biologia Plantarum, 2010,54(3):571-574.
[27]	聂磊,刘鸿先,彭少麟.增强UV-B辐射对柚树苗生长和生理特性效应研究[J].生态科学,2001,20(3):31-38.
[28]	罗丽琼陈宗瑜.紫外线-B辐射对植物DNA及蛋白质的影响[J].生态学杂志,2006,25(5):572-576.
[29]	刘敏,李荣贵,范海,等.UV-B辐射对烟草光合色素和几种酶的影响[J].西北植物学报,2007,27(2):291-296.
[30]	郭欣慰,黄丛林,吴忠义,等.植物类黄酮生物合成的分子调控[J].北方园艺2011(04):204-207.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Effect of Ultraviolet Radiation on Cunninghamia lanceolata Somatic Embryogenesis Plantlets

1. College of Forestry, Nanjing Forestry University, Nanjing 210037, Jiangsu, China

2. Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 2100037, Jiangsu, China

Received Date: 2015-03-07

Abstract: To study the effect of ultraviolet radiation on Chinese fir(Cunninghamia lanceolata(Lamb.) Hook), the somatic embryogenic plants(emblings) regenerated from Chinese fir were used as testing materials. The emblings were treated by different exposure days under ultraviolet light. The results showed that with the prolonging of exposure time under ultraviolet light, the chlorophyll concentration and chlorophyll fluorescence parameters decreased, while the MDA content increased with the activity of POD and soluble protein. It was revealed that the UV radiation could cause a large amount of MDA in leaf which may damage the cell membranes and further decrease the efficiency of photosynthesis of Chinese fir. Stresses may triggered the emblings responding pathway to protect damages by UV radiation through activating POD competence and soluble protein, but failed to save the plants under sever damages in the investigation, POD may excited the activity soluble protein to protect emblings avoiding from damage caused by ultraviolet radiation, but could not being effectively alleviate.

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Reference (30)

[1]	Mckenzie RL, Aucamp PJ, Bais AF,et al. Changes in biologically-active ultraviolet Radiation reaching the earth's surface[J]. Photochemical and Photobiological Science,2007,6(3):218-231.
[2]	郑有军,刘建飞,王艳娜,等.增强UV-B辐射与其它因子符合作用对植物生长的影响研究[J].西北植物学报,2007,27(8):1702-1712.
[3]	蔡锡安,夏汉平,彭少麟.增强UV-B辐射对植物的影响[J].生态环境,2007,16(3):1044-1052.
[4]	ZHU Peng-jin, YANG Li. Ambient UV-B radiation inhibits the growth and physiology of Brassica napus L. on the Qinghai Tibetan plateau[J].Field crops Research,2015,171:79-85.
[5]	Anclia G, Dobribova, Vassilena Krasteva. Damage and protection of the photosynthetic apparatus from UV-B radiation. I. Effect of ascorbate[J].Journal of plant physiology,2013, 170:251-257.
[6]	GAO Qin, ZHANG Li-Xin. Ultraviolent-B-induced oxidative stress and antioxidant defense systemresponses in ascorbate-deficient vtc1 mutant of Arabidopsis thaliana[J].J plant Physiol.2008,165(2):138-148.
[7]	Zlatko S,Zlatev,Fernando J. C. Plant physiological responses to UV-B radiation[J].Emirates Journal of Food & Agriculture, 2012. 24(6):481-501.
[8]	熊日荣.不同杉木无性系在若干逆境下叶绿素荧光参数的比较分析.福建:福建农林大学,2011.
[9]	曲弈,吴华,施季森,等.体胚再生植株低温胁迫的响应机制[J].林业科技开发,2014(05):49-52.
[10]	陈金慧,周小红,施季森.杉木体细胞胚胎发生及植株再生方法,中国,201010275858.0. 2010.
[11]	高俊风.植物生理学实验指导[M].北京:高等教育出版社,2004:211-221.
[12]	张志良,霍伟菁.植物生理学实验指导[M].北京:高等教育出版社,2004,67-69.
[13]	李合生.植物生理生化实验理论与技术[M].北京:高等教育出版社,2001:216-220.
[14]	路文静,李弈松.植物生理学实验指导[M].北京:中国林业出版社,2012:169-233.
[15]	张莉娜,安黎哲,冯虎元,等.增强UV-B辐射和干旱对春小麦光合作用及其生长的影响[J].西北植物学报,2010,30(5):981-986.
[16]	师生波,尚艳霞,朱鹏锦,等.增补UV-B辐射对高山植物美丽风毛菊光合作用和光合色素的影响[J].草地学报,2010,18(5):607-614.
[17]	陈隆升,陈永忠,彭邵锋,等.油茶对低磷胁迫的生理生化效应研究[J].林业科学研究,2010,23(5):782-786.
[18]	白志英,李存东,赵金锋.干旱胁迫对小麦代换系叶绿素荧光参数的影响及染色体效应初步分析[J].中国农业科学,2011,44(1):47-57.
[19]	褚建民,孟平,张劲松,等.土壤水分胁迫对欧李幼苗光合及叶绿素荧光特性的影响[J].林业科学研究,2008,21(3):295-300.
[20]	SUN Zhi-Yang, JI Kong-Shu. Effects of drought stress on chlorophyll fluorescence of four hybrid tulip clones[J].North-West for University, 2010, 25(4):35-39.
[21]	王兆,刘晓曦,郑国华.低温胁迫对彩叶草光合作用及叶绿素荧光的影响[J].浙江农业学报,2015,27(1):49-56.
[22]	黄亚成,秦云霞.植物中活性氧的研究进展[J].中国农学通报,2012,28(36):219-226.
[23]	孙业民,马兰,李朝周.不同类型酸胁迫对云杉叶细胞膜及其保护系统损伤机制的比较[J].林业科学,2012,48(6):56-61.
[24]	吴建勋,张姗姗.Cr、Co、Pb单一胁迫对浮萍SOD、POD、MDA的影响[J].中国农业通报,2013,29(15):188-194.
[25]	尹永强,胡建斌,邓明军.植物叶片抗氧化系统及其对逆境胁迫的响应研究进展[J].中国农学通报,2007,23(1):105-109.
[26]	Fedinai I, Hidma J, Velitchkova M, et al. UV-B induced stress responses in three rice euhivars[J]. Biologia Plantarum, 2010,54(3):571-574.
[27]	聂磊,刘鸿先,彭少麟.增强UV-B辐射对柚树苗生长和生理特性效应研究[J].生态科学,2001,20(3):31-38.
[28]	罗丽琼陈宗瑜.紫外线-B辐射对植物DNA及蛋白质的影响[J].生态学杂志,2006,25(5):572-576.
[29]	刘敏,李荣贵,范海,等.UV-B辐射对烟草光合色素和几种酶的影响[J].西北植物学报,2007,27(2):291-296.
[30]	郭欣慰,黄丛林,吴忠义,等.植物类黄酮生物合成的分子调控[J].北方园艺2011(04):204-207.

Effect of Ultraviolet Radiation on Cunninghamia lanceolata Somatic Embryogenesis Plantlets

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通讯作者: 陈斌, bchen63@163.com

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Effect of Ultraviolet Radiation on Cunninghamia lanceolata Somatic Embryogenesis Plantlets

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