Over-expressing FBL1 Receptor Led to Root Formation and Growth of Populus alba&#215;P. glandulosa cl.'84K'

SHU Wen-bo; ZHAO Shu-tang; ZHANG Jing-jing; ZHOU Yi-hua; LU Meng-zhu

Volume 28 Issue 6

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Over-expressing FBL1 Receptor Led to Root Formation and Growth of Populus alba×P. glandulosa cl.'84K'

1.
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, Jiangsu, China
2.
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Received Date: 2015-06-15

Abstract

Poplar is one of the major fast-growing tree species in China, but little was known about the effect of auxin on fast growth and secondary growth of poplar plants. In this study, PtrFBL1 for auxin receptor gene was cloned in poplar, the over-expression vector of PMDC32-PtrFBL1 based on PMDC32 was constructed and 17PtrFBL1 over-expressed plants of P. alba × P. glandulosa cl. '84K' were obtained. The root, growth and photosynthetic indexes of 3 transgenic lines and the control were analyzed. The results showed that the total root length and total root area of transgenic lines were higher than those of the non-transgenic control lines, and the differences were all significant or extremely significant. On the other hand, the mean adventitious root length, mean adventitious root diameter and root biomass were not significant. The plant height, average internode length, average internode diameter and ratio of height and diameter were higher than these of the control lines. In addition to lower limitation of stoma(Ls), all the other indexes, i.e. the stomatal conductance(Cd), water use efficiency(WUE), light use efficiency(LUE) and relative contents of chlorophyll were higher than those of the controls, and the differences from most transgenic lines reached significant or extremely significant levels. The transgenic lines increased the root areas, and had stronger photosynthetic efficiency and rate of water consumption, and promoted biomass accumulation on ground, thus had the accelerated speed of growth. Therefore, the PtrFBL1 gene has the potential to be manipulated to improve the growth.
- over-expressing,
- FBL1,
- Populus alba ×,
- Populus glandulosa cl. '84K',
- root formation

References

[1]	Lau S, Shao N, Bock R, et al. Auxin signaling in algal lineages:fact or myth?[J].Trends in plant science, 2009, 14(4):182-188.
[2]	Salehin, M., Bagchi, R., Estelle, M. SCF^TIR1/AFB-based auxin perception:mechanism and role in plant growth and development[J]. The Plant Cell, 2015, 27(1):9-19.
[3]	Yu H, Moss B L, Jang S S, et al. Mutations in the TIR1 auxin receptor that increase affinity for auxin/indole-3-acetic acid proteins result in auxin hypersensitivity[J]. Plant physiology, 2013, 162(1):295-303.
[4]	Villalobos L I A C, Lee S, De Oliveira C, et al. A combinatorial TIR1/AFB-Aux/IAA co-receptor system for differential sensing of auxin[J]. Nature Chemical Biology, 2012, 8(5):477-485.
[5]	Dharmasiri N, Dharmasiri S, Estelle M. The F-box protein TIR1 is an auxin receptor[J]. Nature, 2005, 435:441-445.
[6]	Parry G, Calderon-Villalobos L, Prigge M, et al. Complex regulation of the TIR1/AFB family of auxin receptors[J]. Proceedings of the National Academy of Sciences, 2009, 106:22540-22545.
[7]	Hu Z, Keçeli M A, Piisilä M, et al. F-box protein AFB4 plays a crucial role in plant growth, development and innate immunity[J]. Cell research, 2012, 22:777-781.
[8]	Ruegger M, Dewey E, Gray W M, et al. The TIR1 protein of Arabidopsis functions in auxin response and is related to human SKP2 and yeast Grr1p[J]. Genes & Development, 1998, 12(2):198-207.
[9]	Ren Z, Li Z, Miao Q, et al. The auxin receptor homologue in Solanum lycopersicum stimulates tomato fruit set and leaf morphogenesis[J]. Journal of experimental botany, 2011, 62(8):2815-2826.
[10]	Bian H, Xie Y, Guo F, et al. Distinctive expression patterns and roles of the miRNA393/TIR1 homolog module in regulating flag leaf inclination and primary and crown root growth in rice(Oryza sativa)[J]. New Phytologist, 2012, 196(1):149-161.
[11]	甘肖梅.桂林岩溶石山阴香光合生理生态特性研究.广西:广西师范大学硕士论文, 2010.
[12]	Peer W A. From perception to attenuation:auxin signalling and responses[J]. Current opinion in plant biology,2013,16(5):561-568.
[13]	Jurado S, Díaz-Triviño S, Abraham Z, et al. SKP2A, an F-box protein that regulates cell division, is degraded via the ubiquitin pathway[J]. The Plant Journal, 2008, 53(5):828-841.
[14]	Ljung K. Auxin metabolism and homeostasis during plant development[J]. Development, 2013, 40:943-950.
[15]	Xie Q, Frugis G, Colgan D, et al. Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development[J]. Genes & Development, 2000, 14(23):3024-3036.
[16]	Guseman J M, Hellmuth A, Lanctot A, et al. Auxin-induced degradation dynamics set the pace for lateral root development[J]. Development, 2015, 142(5):905-909.
[17]	Piya S, Shrestha S K, Binder B, et al. Protein-protein interaction and gene co-expression maps of ARFs and Aux/IAAs in Arabidopsis[J]. Frontiers in Plant Science, 2014, 5:1-9.
[18]	Gupta A, Singh M, Laxmi A. Interaction between glucose and brassinosteroid during the regulation of lateral root development in Arabidopsis[J]. Plant Physiology, 2015, 168(1):307-320.
[19]	Xia K, Wang R, Ou X, et al. OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice[J]. PLoS One, 2012, 7(1):e30039.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Over-expressing FBL1 Receptor Led to Root Formation and Growth of Populus alba×P. glandulosa cl.'84K'

1. Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, Jiangsu, China

2. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Received Date: 2015-06-15

Abstract: Poplar is one of the major fast-growing tree species in China, but little was known about the effect of auxin on fast growth and secondary growth of poplar plants. In this study, PtrFBL1 for auxin receptor gene was cloned in poplar, the over-expression vector of PMDC32-PtrFBL1 based on PMDC32 was constructed and 17PtrFBL1 over-expressed plants of P. alba × P. glandulosa cl. '84K' were obtained. The root, growth and photosynthetic indexes of 3 transgenic lines and the control were analyzed. The results showed that the total root length and total root area of transgenic lines were higher than those of the non-transgenic control lines, and the differences were all significant or extremely significant. On the other hand, the mean adventitious root length, mean adventitious root diameter and root biomass were not significant. The plant height, average internode length, average internode diameter and ratio of height and diameter were higher than these of the control lines. In addition to lower limitation of stoma(Ls), all the other indexes, i.e. the stomatal conductance(Cd), water use efficiency(WUE), light use efficiency(LUE) and relative contents of chlorophyll were higher than those of the controls, and the differences from most transgenic lines reached significant or extremely significant levels. The transgenic lines increased the root areas, and had stronger photosynthetic efficiency and rate of water consumption, and promoted biomass accumulation on ground, thus had the accelerated speed of growth. Therefore, the PtrFBL1 gene has the potential to be manipulated to improve the growth.

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[1]	Lau S, Shao N, Bock R, et al. Auxin signaling in algal lineages:fact or myth?[J].Trends in plant science, 2009, 14(4):182-188.
[2]	Salehin, M., Bagchi, R., Estelle, M. SCF^TIR1/AFB-based auxin perception:mechanism and role in plant growth and development[J]. The Plant Cell, 2015, 27(1):9-19.
[3]	Yu H, Moss B L, Jang S S, et al. Mutations in the TIR1 auxin receptor that increase affinity for auxin/indole-3-acetic acid proteins result in auxin hypersensitivity[J]. Plant physiology, 2013, 162(1):295-303.
[4]	Villalobos L I A C, Lee S, De Oliveira C, et al. A combinatorial TIR1/AFB-Aux/IAA co-receptor system for differential sensing of auxin[J]. Nature Chemical Biology, 2012, 8(5):477-485.
[5]	Dharmasiri N, Dharmasiri S, Estelle M. The F-box protein TIR1 is an auxin receptor[J]. Nature, 2005, 435:441-445.
[6]	Parry G, Calderon-Villalobos L, Prigge M, et al. Complex regulation of the TIR1/AFB family of auxin receptors[J]. Proceedings of the National Academy of Sciences, 2009, 106:22540-22545.
[7]	Hu Z, Keçeli M A, Piisilä M, et al. F-box protein AFB4 plays a crucial role in plant growth, development and innate immunity[J]. Cell research, 2012, 22:777-781.
[8]	Ruegger M, Dewey E, Gray W M, et al. The TIR1 protein of Arabidopsis functions in auxin response and is related to human SKP2 and yeast Grr1p[J]. Genes & Development, 1998, 12(2):198-207.
[9]	Ren Z, Li Z, Miao Q, et al. The auxin receptor homologue in Solanum lycopersicum stimulates tomato fruit set and leaf morphogenesis[J]. Journal of experimental botany, 2011, 62(8):2815-2826.
[10]	Bian H, Xie Y, Guo F, et al. Distinctive expression patterns and roles of the miRNA393/TIR1 homolog module in regulating flag leaf inclination and primary and crown root growth in rice(Oryza sativa)[J]. New Phytologist, 2012, 196(1):149-161.
[11]	甘肖梅.桂林岩溶石山阴香光合生理生态特性研究.广西:广西师范大学硕士论文, 2010.
[12]	Peer W A. From perception to attenuation:auxin signalling and responses[J]. Current opinion in plant biology,2013,16(5):561-568.
[13]	Jurado S, Díaz-Triviño S, Abraham Z, et al. SKP2A, an F-box protein that regulates cell division, is degraded via the ubiquitin pathway[J]. The Plant Journal, 2008, 53(5):828-841.
[14]	Ljung K. Auxin metabolism and homeostasis during plant development[J]. Development, 2013, 40:943-950.
[15]	Xie Q, Frugis G, Colgan D, et al. Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development[J]. Genes & Development, 2000, 14(23):3024-3036.
[16]	Guseman J M, Hellmuth A, Lanctot A, et al. Auxin-induced degradation dynamics set the pace for lateral root development[J]. Development, 2015, 142(5):905-909.
[17]	Piya S, Shrestha S K, Binder B, et al. Protein-protein interaction and gene co-expression maps of ARFs and Aux/IAAs in Arabidopsis[J]. Frontiers in Plant Science, 2014, 5:1-9.
[18]	Gupta A, Singh M, Laxmi A. Interaction between glucose and brassinosteroid during the regulation of lateral root development in Arabidopsis[J]. Plant Physiology, 2015, 168(1):307-320.
[19]	Xia K, Wang R, Ou X, et al. OsTIR1 and OsAFB2 downregulation via OsmiR393 overexpression leads to more tillers, early flowering and less tolerance to salt and drought in rice[J]. PLoS One, 2012, 7(1):e30039.

Over-expressing FBL1 Receptor Led to Root Formation and Growth of Populus alba×P. glandulosa cl.'84K'

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通讯作者: 陈斌, bchen63@163.com

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Over-expressing FBL1 Receptor Led to Root Formation and Growth of Populus alba×P. glandulosa cl.'84K'

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