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Citation:

Genetic Variation and Conservation Units Analysis in Pinus yunnanensis Natural Populations

  • Received Date: 2015-06-12
  • The intra-specific variation of 20Pinus yunnanensis Franch. natural populations collected from the main distribution areas was analyzed. The results revealed higher genetic variation within populations than among populations based on SSR data and the genetic variation existed mainly within populations. There were not significantly genetic differences among populations. Based on SSR data and Nei's genetic similarity and the level of genetic diversity, the conservation units of P. yunnanensis were analyzed by using stepwise clustering and preferred sampling. The representativeness of sampling were evaluated by T-test among primary populations, conservation units and remainder populations. The results showed this method was effective to establish the conservation units. The CU6 composed of six P. yunnanensis natural populations had the 30% germplasm samples of primary populations. The retention ratio of number of alleles, effective number of alleles, Shannon's information index and expected heterozygosity were 98.03%, 105.36%, 103.99% and 105.56%, respectively. The genetic diversity of conservation units was higher than the remainder populations, which indicated that the populations sampled from primary populations for conversation units were representative and effective.
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Genetic Variation and Conservation Units Analysis in Pinus yunnanensis Natural Populations

  • 1. Southwest Forestry University, Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province, Kunming 650224, Yunnan, China
  • 2. Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming 650204, Yunnan, China
  • 3. Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, Yunnan, China

Abstract: The intra-specific variation of 20Pinus yunnanensis Franch. natural populations collected from the main distribution areas was analyzed. The results revealed higher genetic variation within populations than among populations based on SSR data and the genetic variation existed mainly within populations. There were not significantly genetic differences among populations. Based on SSR data and Nei's genetic similarity and the level of genetic diversity, the conservation units of P. yunnanensis were analyzed by using stepwise clustering and preferred sampling. The representativeness of sampling were evaluated by T-test among primary populations, conservation units and remainder populations. The results showed this method was effective to establish the conservation units. The CU6 composed of six P. yunnanensis natural populations had the 30% germplasm samples of primary populations. The retention ratio of number of alleles, effective number of alleles, Shannon's information index and expected heterozygosity were 98.03%, 105.36%, 103.99% and 105.56%, respectively. The genetic diversity of conservation units was higher than the remainder populations, which indicated that the populations sampled from primary populations for conversation units were representative and effective.

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