Analysis of MYB Transcription Factor Involved in Development of Secondary Vascular System in Tobacco and Populus tomentosa

GUO Wei; ZHAO Shu-tang; LU Meng-zhu

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Analysis of MYB Transcription Factor Involved in Development of Secondary Vascular System in Tobacco and Populus tomentosa

1.
Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Received Date: 2013-05-14

Abstract

Tobacco plants have typical secondary vascular development process and can be used to study the function of genes related to secondary vascular development. 48 pairs of MYB orthologous genes obtained from tobacco and poplar were identified by searching in the entire poplar genome sequence with MYB transcription factor obtained from transcriptome of tobacco vascular tissues as probes, and were divided into 23 subgroups. The analysis of gene expression levels obtained using RNA-seq in tobacco and poplar suggested that 15 pairs of MYB orthologous genes shared similar expression patterns both in tobacco and poplar. The results of expression analysis using real time RT-PCR in different tissues of tobacco and poplar indicated that the MYB orthologous genes shared similar expression patterns both in tobacco and poplar. It is thus feasible to rapidly analyze the function of MYB transcription factors in vascular development process with tobacco.
- tobacco,
- Populus tomentosa,
- MYB,
- secondary growth

References

[1]	Plomion C,Leprovost G,Stoke A. Wood Formation in Trees[J]. Plant Physiology, 2001,127(4):1513-1523.
[2]	Groover A T. What genes make a tree a tree?[J]. Trends Plant Sci, 2005,10(5):210-214.
[3]	Boerjan W,Ralph J,Baucher M. Lignin biosynthesis[J]. Annual review of plant biology, 2003,54:519-546.
[4]	Wang M,Qi X,Zhao S. Dynamic changes in transcripts during regeneration of the secondary vascular system in Populus tomentosa Carr. revealed by cDNA microarrays[J]. BMC genomics, 2009,10:215.
[5]	Schrader J,Nilsson J,Mellerowicz E. A high-resolution transcript profile across the wood-forming meristem of poplar identifies potential regulators of cambial stem cell identity[J]. The Plant cell, 2004,16(9):2278-2292.
[6]	NairnCJ,Lennon, D M,Wood-Jones A. Carbohydrate-related genes and cell wall biosynthesis in vascular tissues of loblolly pine (Pinus taeda)[J]. Tree physiology, 2008,28(7):1099-1110.
[7]	Yang J,KamdemD P,Keathley D E. Seasonal changes in gene expression at the sapwood-heartwood transition zone of black locust (Robinia pseudoacacia) revealed by cDNA microarray analysis[J]. Tree physiology, 2004,24(4):461-474.
[8]	Paux E,Carocha V,Marques C. Transcript profiling of Eucalyptus xylem genes during tension wood formation[J]. The New phytologist, 2005,167(1):89-100.
[9]	Pavy N,BoyleB,Nelson C. Identification of conserved core xylem gene sets: conifer cDNA microarray development, transcript profiling and computational analyses[J]. The New phytologist, 2008,180(4):766-786.
[10]	Du J,Xie H L,Zhang D Q. Regeneration of the secondary vascular system in poplar as a novel system to investigate gene expression by a proteomic approach[J]. Proteomics, 2006,6(3):881-895.
[11]	Lipsick J S. One billion years of Myb[J]. Oncogene, 1996,13(2):223-235.
[12]	Stracke R,Werber M,Weisshaar B. The R2R3-MYB gene family in Arabidopsis thaliana[J]. Curr Opin Plant Biol, 2001,4(5):447-456.
[13]	Ogata K,Morikawa S,Nakamura H. Comparison of the free and DNA-complexed forms of the DNA-binding domain from c-Myb[J]. Nature structural biology, 1995,2(4):309-320.
[14]	Ma Q H,Wang C,Zhu H H. TaMYB4 cloned from wheat regulates lignin biosynthesis through negatively controlling the transcripts of both cinnamyl alcohol dehydrogenase and cinnamoyl-CoA reductase genes[J]. Biochimie, 2011,93(7):1179-1186.
[15]	Zhong R,Lee C,Zhou J. A battery of transcription factors involved in the regulation of secondary cell wall biosynthesis in Arabidopsis[J]. The Plant cell, 2008,20(10):2763-2782.
[16]	Nieminen K M,Kauppinen L,Helariutta Y. A weed for wood? Arabidopsis as a genetic model for xylem development[J]. Plant Physiol, 2004,135(2):653-659.
[17]	Cook C M,Daudi A,Millar D J. Transcriptional changes related to secondary wall formation in xylem of transgenic lines of tobacco altered for lignin or xylan content which show improved saccharification[J]. Phytochemistry, 2012,74:79-89.
[18]	Al-Ahmad H,Galili S,Gressel J. Tandem constructs to mitigate transgene persistence: tobacco as a model[J]. Molecular Ecology, 2004,13(3):697-710.
[19]	Huang C J,Zhang T,Li F F. Development and application of an efficient virus-induced gene silencing system in Nicotiana tabacum using geminivirus alphasatellite[J]. Journal of Zhejiang University Science B, 2011,12(2):83-92.
[20]	Zhu X,Pattathil S,Mazumder K. Virus-induced gene silencing offers a functional genomics platform for studying plant cell wall formation[J]. Molecular plant, 2010,3(5):818-833.
[21]	Mortazavi A,Williams B A,McCue K. Mapping and quantifying mammalian transcriptomes by RNA-Seq[J]. Nature methods, 2008,5(7):621-628.
[22]	Livak K J,Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods, 2001,25(4):402-408.
[23]	Dubos C,Stracke R,Grotewold E. MYB transcription factors in Arabidopsis[J]. Trends Plant Sci, 2010,15(10):573-581.
[24]	Newman L J,Perazza D E,Juda L. Involvement of the R2R3-MYB, AtMYB61, in the ectopic lignification and dark-photomorphogenic components of the det3 mutant phenotype[J]. The Plant journal : for cell and molecular biology, 2004,37(2):239-250.
[25]	Reyes J L,Chua N H. ABA induction of miR159 controls transcript levels of two MYB factors during Arabidopsis seed germination[J]. The Plant journal : for cell and molecular biology, 2007,49(4):592-606.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Analysis of MYB Transcription Factor Involved in Development of Secondary Vascular System in Tobacco and Populus tomentosa

1. Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Received Date: 2013-05-14

Abstract: Tobacco plants have typical secondary vascular development process and can be used to study the function of genes related to secondary vascular development. 48 pairs of MYB orthologous genes obtained from tobacco and poplar were identified by searching in the entire poplar genome sequence with MYB transcription factor obtained from transcriptome of tobacco vascular tissues as probes, and were divided into 23 subgroups. The analysis of gene expression levels obtained using RNA-seq in tobacco and poplar suggested that 15 pairs of MYB orthologous genes shared similar expression patterns both in tobacco and poplar. The results of expression analysis using real time RT-PCR in different tissues of tobacco and poplar indicated that the MYB orthologous genes shared similar expression patterns both in tobacco and poplar. It is thus feasible to rapidly analyze the function of MYB transcription factors in vascular development process with tobacco.

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Reference (25)

[1]	Plomion C,Leprovost G,Stoke A. Wood Formation in Trees[J]. Plant Physiology, 2001,127(4):1513-1523.
[2]	Groover A T. What genes make a tree a tree?[J]. Trends Plant Sci, 2005,10(5):210-214.
[3]	Boerjan W,Ralph J,Baucher M. Lignin biosynthesis[J]. Annual review of plant biology, 2003,54:519-546.
[4]	Wang M,Qi X,Zhao S. Dynamic changes in transcripts during regeneration of the secondary vascular system in Populus tomentosa Carr. revealed by cDNA microarrays[J]. BMC genomics, 2009,10:215.
[5]	Schrader J,Nilsson J,Mellerowicz E. A high-resolution transcript profile across the wood-forming meristem of poplar identifies potential regulators of cambial stem cell identity[J]. The Plant cell, 2004,16(9):2278-2292.
[6]	NairnCJ,Lennon, D M,Wood-Jones A. Carbohydrate-related genes and cell wall biosynthesis in vascular tissues of loblolly pine (Pinus taeda)[J]. Tree physiology, 2008,28(7):1099-1110.
[7]	Yang J,KamdemD P,Keathley D E. Seasonal changes in gene expression at the sapwood-heartwood transition zone of black locust (Robinia pseudoacacia) revealed by cDNA microarray analysis[J]. Tree physiology, 2004,24(4):461-474.
[8]	Paux E,Carocha V,Marques C. Transcript profiling of Eucalyptus xylem genes during tension wood formation[J]. The New phytologist, 2005,167(1):89-100.
[9]	Pavy N,BoyleB,Nelson C. Identification of conserved core xylem gene sets: conifer cDNA microarray development, transcript profiling and computational analyses[J]. The New phytologist, 2008,180(4):766-786.
[10]	Du J,Xie H L,Zhang D Q. Regeneration of the secondary vascular system in poplar as a novel system to investigate gene expression by a proteomic approach[J]. Proteomics, 2006,6(3):881-895.
[11]	Lipsick J S. One billion years of Myb[J]. Oncogene, 1996,13(2):223-235.
[12]	Stracke R,Werber M,Weisshaar B. The R2R3-MYB gene family in Arabidopsis thaliana[J]. Curr Opin Plant Biol, 2001,4(5):447-456.
[13]	Ogata K,Morikawa S,Nakamura H. Comparison of the free and DNA-complexed forms of the DNA-binding domain from c-Myb[J]. Nature structural biology, 1995,2(4):309-320.
[14]	Ma Q H,Wang C,Zhu H H. TaMYB4 cloned from wheat regulates lignin biosynthesis through negatively controlling the transcripts of both cinnamyl alcohol dehydrogenase and cinnamoyl-CoA reductase genes[J]. Biochimie, 2011,93(7):1179-1186.
[15]	Zhong R,Lee C,Zhou J. A battery of transcription factors involved in the regulation of secondary cell wall biosynthesis in Arabidopsis[J]. The Plant cell, 2008,20(10):2763-2782.
[16]	Nieminen K M,Kauppinen L,Helariutta Y. A weed for wood? Arabidopsis as a genetic model for xylem development[J]. Plant Physiol, 2004,135(2):653-659.
[17]	Cook C M,Daudi A,Millar D J. Transcriptional changes related to secondary wall formation in xylem of transgenic lines of tobacco altered for lignin or xylan content which show improved saccharification[J]. Phytochemistry, 2012,74:79-89.
[18]	Al-Ahmad H,Galili S,Gressel J. Tandem constructs to mitigate transgene persistence: tobacco as a model[J]. Molecular Ecology, 2004,13(3):697-710.
[19]	Huang C J,Zhang T,Li F F. Development and application of an efficient virus-induced gene silencing system in Nicotiana tabacum using geminivirus alphasatellite[J]. Journal of Zhejiang University Science B, 2011,12(2):83-92.
[20]	Zhu X,Pattathil S,Mazumder K. Virus-induced gene silencing offers a functional genomics platform for studying plant cell wall formation[J]. Molecular plant, 2010,3(5):818-833.
[21]	Mortazavi A,Williams B A,McCue K. Mapping and quantifying mammalian transcriptomes by RNA-Seq[J]. Nature methods, 2008,5(7):621-628.
[22]	Livak K J,Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods, 2001,25(4):402-408.
[23]	Dubos C,Stracke R,Grotewold E. MYB transcription factors in Arabidopsis[J]. Trends Plant Sci, 2010,15(10):573-581.
[24]	Newman L J,Perazza D E,Juda L. Involvement of the R2R3-MYB, AtMYB61, in the ectopic lignification and dark-photomorphogenic components of the det3 mutant phenotype[J]. The Plant journal : for cell and molecular biology, 2004,37(2):239-250.
[25]	Reyes J L,Chua N H. ABA induction of miR159 controls transcript levels of two MYB factors during Arabidopsis seed germination[J]. The Plant journal : for cell and molecular biology, 2007,49(4):592-606.

Analysis of MYB Transcription Factor Involved in Development of Secondary Vascular System in Tobacco and Populus tomentosa

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通讯作者: 陈斌, bchen63@163.com

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Analysis of MYB Transcription Factor Involved in Development of Secondary Vascular System in Tobacco and Populus tomentosa

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