High-throughput Transcriptome Identification and Flavonoids Metabolic Pathways in Nitraria sphaerocarpa

MA Jing; DENG Nan; CHU Jian-min; JI Jing; SHI Sheng-qing; JIANG Ze-ping; CHENG Tie-long

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High-throughput Transcriptome Identification and Flavonoids Metabolic Pathways in Nitraria sphaerocarpa

1.
Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China
2.
Nanjing Forestry University, Nanjing 210037, Jiangsu, China

Received Date: 2015-07-06

Abstract

[Objective]To understand the biosynthetic pathway of flavonoids and its roles in the adaptation to abiotic stresses in Nitraria sphaerocarpa. [Method]High-throughput RNA-seq technology was used to generate the transcriptome of current-year leaves, and then the related bioinformatics analysis was performed. [Result]The results showed that 13 013 444 raw reads and 48 921 unigenes were acquired after de novo assembly; 30 407 annotations were acquired from Nr database, 19 671 from Swiss-Prot database, 9 273 from COG database, 46 153 from GO database and 13 654 from KEGG database after function annotation against five databases; 186 unigenes were associated with flavonoid biosynthesisby the analysis of KEGG pathway. [Conclusion]The better transcriptomic data was obtained, and it was rich in the genes involved in flavonoids biosynthesis, which would provide references for the studies of its resistance, medicinal value in future.
- Nitraria sphaerocarpa,
- transcriptome,
- RNA-seq,
- flavonoids

References

[1]	潘晓玲,沈观冕,陈鹏. 白刺属植物的分类学及系统学研究[J]. 云南植物研究,1999,21(3):287-295.
[2]	刘殿君,吴波,李永华,等. 极端干旱区增雨加速泡泡刺群落土壤碳排放[J]. 生态学报,2012,32(17):5396-5404.
[3]	Cheng T, Chen J, Zhang J, et al. Physiological and proteomic analyses of leaves from the halophyte Tangut Nitraria reveals diverse response pathways critical for high salinity tolerance[J]. Front Plant Sci, 2015, 6: 30.
[4]	Li Q Y, Zhao W Z, Fang H Y. Adaptation of Nitraria sphaerocarpa to wind-blown sand environments at the edge of a desert oasis[J]. J Environ Sci (China), 2007, 19(4): 482-487.
[5]	Gravel E, Poupon E. Biosynthesis and biomimetic synthesis of alkaloids isolated from plants of the Nitraria and Myrioneuron genera: an unusual lysine-based metabolism[J]. Nat Prod Rep, 2010, 27(1): 32-56.
[6]	Halim A F, Saad H E, Hashish N E. Flavonol glycosides from Nitraria retusa[J]. Phytochemistry, 1995, 40(1): 349-351.
[7]	Du J, Yan P, Dong Y, et al. Phenological response of Nitraria tangutorum to climate change in Minqin County, Gansu Province, Northwest China[J]. Int J Biometeorol, 2010,54(5): 583-593.
[8]	白潇,李毅,苏世平,等. 不同居群唐古特白刺叶片解剖特征对生境的响应研究[J]. 西北植物学报,2013,23(10):1986-1993.
[9]	张艳艳,刘威,宣亚楠,等. 水杨酸对盐胁迫下唐古特白刺活性氧代谢和细胞膜透性抑制的缓解效应[J]. 东北林业大学学报,2013,41(12):56-59.
[10]	唐欣,王瑞辉,杨秀艳,等. 唐古特白刺液泡膜Na⁺/H⁺逆向运输蛋白基因NtNHX1的克隆与表达分析[J]. 林业科学,2014,27(3):38-44.
[11]	Chen J, Cheng T, Wang P, et al. Salinity-induced changes in protein expression in the halophytic plant Nitraria sphaerocarpa[J]. J Proteomics, 2012, 75(17): 5226-5243.
[12]	Saija A, Scalese M, Lanza M, et al. Flavonoids as antioxidant agents: importance of their interaction with biomembranes[J]. Free Radic Biol Med, 1995, 19(4): 481-486.
[13]	Cheynier V, Comte G, Davies K M, et al. Plant phenolics: recent advances on their biosynthesis, genetics, and ecophysiology[J]. Plant Physiol Biochem, 2013,72:1-20.
[14]	文海涛,赵红英,肖凤霞,等. 化州柚查尔酮合成酶基因克隆与序列分析[J]. 生物学杂志,2011,28(1):39-41.
[15]	Shimada N. A cluster of genes encodes the two types of chalcone is omerase involved in the biosynthesis of general falavonoids and legume specific 5-deoxy (iso) flavonoids in Lotus japonicus[J]. Plant Physiol, 2003, 131(3): 941-951.
[16]	张丹凤. 蒙古黄芪异黄酮合成酶基因的克隆及序列分析[D]. 福州:福建农林大学, 2004.
[17]	郝大程,马培,穆军,等. 中药植物虎杖根的高通量转录组测序及转录组特性分析[J]. 中国科学:生命科学,2012,42(5):398-412.
[18]	邓楠,史胜青,常二梅,等. 基于中麻黄萌发种子转录组的黄酮类化合物合成途径基因的挖掘[J]. 林业科学研究,2014, 27(6):758-763.
[19]	邓楠,史胜青,常二梅,等. 膜果麻黄种子不同发育时期的转录组测序分析[J]. 东北林业大学学报,2015,43(2):28-32.
[20]	王洪伦,李玉林,索有瑞. 白刺种子黄酮类化合物最佳提取工艺研究[J]. 食品科学,2004,25(7):97-99.
[21]	高喆,王佳,特布沁,等. 唐古特白刺叶和茎黄酮含量分析[J]. 中国草地学报,2014,36(3):116-120.
[22]	Shi C Y, Yang H, Wei C L, et al. Deep sequencing of the Camellia sinensis transcriptome revealed candidate genes for major metabolic pathways of tea-specific compounds[J]. BMC Genomics, 2011, 12: 131.
[23]	Tang Q, Ma X, Mo C, et al. An efficient approach to finding Siraitia grosvenorii triterpene biosynthetic genes by RNA-seq and digital gene expression analysis[J]. BMC Genomics, 2011,12: 343.
[24]	王晓梅,曹稳根. 黄酮类化合物药理作用的研究进展[J]. 宿州学院学报,2008,22(1):105-107.
[25]	廖头根,汪秋安,方伟琴,等. 新型查尔酮类化合物的合成及其生物活性研究[J]. 有机化学,2006,26(5):685-689.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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High-throughput Transcriptome Identification and Flavonoids Metabolic Pathways in Nitraria sphaerocarpa

1. Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

2. Nanjing Forestry University, Nanjing 210037, Jiangsu, China

Received Date: 2015-07-06

Abstract: [Objective]To understand the biosynthetic pathway of flavonoids and its roles in the adaptation to abiotic stresses in Nitraria sphaerocarpa. [Method]High-throughput RNA-seq technology was used to generate the transcriptome of current-year leaves, and then the related bioinformatics analysis was performed. [Result]The results showed that 13 013 444 raw reads and 48 921 unigenes were acquired after de novo assembly; 30 407 annotations were acquired from Nr database, 19 671 from Swiss-Prot database, 9 273 from COG database, 46 153 from GO database and 13 654 from KEGG database after function annotation against five databases; 186 unigenes were associated with flavonoid biosynthesisby the analysis of KEGG pathway. [Conclusion]The better transcriptomic data was obtained, and it was rich in the genes involved in flavonoids biosynthesis, which would provide references for the studies of its resistance, medicinal value in future.

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Reference (25)

[1]	潘晓玲,沈观冕,陈鹏. 白刺属植物的分类学及系统学研究[J]. 云南植物研究,1999,21(3):287-295.
[2]	刘殿君,吴波,李永华,等. 极端干旱区增雨加速泡泡刺群落土壤碳排放[J]. 生态学报,2012,32(17):5396-5404.
[3]	Cheng T, Chen J, Zhang J, et al. Physiological and proteomic analyses of leaves from the halophyte Tangut Nitraria reveals diverse response pathways critical for high salinity tolerance[J]. Front Plant Sci, 2015, 6: 30.
[4]	Li Q Y, Zhao W Z, Fang H Y. Adaptation of Nitraria sphaerocarpa to wind-blown sand environments at the edge of a desert oasis[J]. J Environ Sci (China), 2007, 19(4): 482-487.
[5]	Gravel E, Poupon E. Biosynthesis and biomimetic synthesis of alkaloids isolated from plants of the Nitraria and Myrioneuron genera: an unusual lysine-based metabolism[J]. Nat Prod Rep, 2010, 27(1): 32-56.
[6]	Halim A F, Saad H E, Hashish N E. Flavonol glycosides from Nitraria retusa[J]. Phytochemistry, 1995, 40(1): 349-351.
[7]	Du J, Yan P, Dong Y, et al. Phenological response of Nitraria tangutorum to climate change in Minqin County, Gansu Province, Northwest China[J]. Int J Biometeorol, 2010,54(5): 583-593.
[8]	白潇,李毅,苏世平,等. 不同居群唐古特白刺叶片解剖特征对生境的响应研究[J]. 西北植物学报,2013,23(10):1986-1993.
[9]	张艳艳,刘威,宣亚楠,等. 水杨酸对盐胁迫下唐古特白刺活性氧代谢和细胞膜透性抑制的缓解效应[J]. 东北林业大学学报,2013,41(12):56-59.
[10]	唐欣,王瑞辉,杨秀艳,等. 唐古特白刺液泡膜Na⁺/H⁺逆向运输蛋白基因NtNHX1的克隆与表达分析[J]. 林业科学,2014,27(3):38-44.
[11]	Chen J, Cheng T, Wang P, et al. Salinity-induced changes in protein expression in the halophytic plant Nitraria sphaerocarpa[J]. J Proteomics, 2012, 75(17): 5226-5243.
[12]	Saija A, Scalese M, Lanza M, et al. Flavonoids as antioxidant agents: importance of their interaction with biomembranes[J]. Free Radic Biol Med, 1995, 19(4): 481-486.
[13]	Cheynier V, Comte G, Davies K M, et al. Plant phenolics: recent advances on their biosynthesis, genetics, and ecophysiology[J]. Plant Physiol Biochem, 2013,72:1-20.
[14]	文海涛,赵红英,肖凤霞,等. 化州柚查尔酮合成酶基因克隆与序列分析[J]. 生物学杂志,2011,28(1):39-41.
[15]	Shimada N. A cluster of genes encodes the two types of chalcone is omerase involved in the biosynthesis of general falavonoids and legume specific 5-deoxy (iso) flavonoids in Lotus japonicus[J]. Plant Physiol, 2003, 131(3): 941-951.
[16]	张丹凤. 蒙古黄芪异黄酮合成酶基因的克隆及序列分析[D]. 福州:福建农林大学, 2004.
[17]	郝大程,马培,穆军,等. 中药植物虎杖根的高通量转录组测序及转录组特性分析[J]. 中国科学:生命科学,2012,42(5):398-412.
[18]	邓楠,史胜青,常二梅,等. 基于中麻黄萌发种子转录组的黄酮类化合物合成途径基因的挖掘[J]. 林业科学研究,2014, 27(6):758-763.
[19]	邓楠,史胜青,常二梅,等. 膜果麻黄种子不同发育时期的转录组测序分析[J]. 东北林业大学学报,2015,43(2):28-32.
[20]	王洪伦,李玉林,索有瑞. 白刺种子黄酮类化合物最佳提取工艺研究[J]. 食品科学,2004,25(7):97-99.
[21]	高喆,王佳,特布沁,等. 唐古特白刺叶和茎黄酮含量分析[J]. 中国草地学报,2014,36(3):116-120.
[22]	Shi C Y, Yang H, Wei C L, et al. Deep sequencing of the Camellia sinensis transcriptome revealed candidate genes for major metabolic pathways of tea-specific compounds[J]. BMC Genomics, 2011, 12: 131.
[23]	Tang Q, Ma X, Mo C, et al. An efficient approach to finding Siraitia grosvenorii triterpene biosynthetic genes by RNA-seq and digital gene expression analysis[J]. BMC Genomics, 2011,12: 343.
[24]	王晓梅,曹稳根. 黄酮类化合物药理作用的研究进展[J]. 宿州学院学报,2008,22(1):105-107.
[25]	廖头根,汪秋安,方伟琴,等. 新型查尔酮类化合物的合成及其生物活性研究[J]. 有机化学,2006,26(5):685-689.

High-throughput Transcriptome Identification and Flavonoids Metabolic Pathways in Nitraria sphaerocarpa

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References

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通讯作者: 陈斌, bchen63@163.com

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High-throughput Transcriptome Identification and Flavonoids Metabolic Pathways in Nitraria sphaerocarpa

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