The Effect of Forest Marsh and Fire Disturbance on Soil Microbial in Greater Xing'an Mountain

LIN Yin-hua; LU Ping; ZHAO Lu-an; TAN Fei; XU Yan-peng; JIA Xu-dong; LI Hui-ren; LIU Xue-shuang; WEI Chang-lei; WANG Li-zhong

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The Effect of Forest Marsh and Fire Disturbance on Soil Microbial in Greater Xing'an Mountain

1.
Institute of Wetland Research, Chinese Academy of Forestry, Beijing 100091
2.
Institute of Heilongjiang Provincial Monitoring and Planning of Forestry, Harbin 154080, Heilongjiang
3.
The Administration Bureau of Jiuwan Mountain National Natural Reserve, Liuzhou 545300, Guangxi
4.
Research Institute of Agriculture and Forestry, Greater xing'an Forestry Group, Jiagedaqi 165000, Heilongjiang

Received Date: 2015-06-03

Abstract

[Objective]The aim of this paper was studied the characteristic of soil microbiological community and its diversity at forest marsh development and fire disturbance, to understand the role of soil microbial community in the forest marsh conservation and restoration.[Methods]We used phospholipid fatty acids (PLFAs) to portrait the community composition and community level physiological profiles (CLPP) to describe the functional diversity of the microbial community. [Results]Total of 5 types of marsh were chosen in Natural reserve area of Nanwenhe in Greater xing'an Mountain, which included xing'an Larch-Ledum palustre-moss marsh, xing'an Larch-xing'an azalea-moss marsh, xing'an Larch-birch-carex marsh, and Larch-xing'an azalea-moss marsh by severe fire disturbance, xing'an Larch-birch-carex marsh by mild fire disturbance at 2006. It was found that the PLFAs of 16:00(16.29±5.62 nmol·g-1), 18:1ω8t(9.89±8.61 nmol·g-1)and 16:1ω7c(9.79±3.24 nmol·g-1)were dominance in microbial community, and the dominant species contributed significantly to variations in soil microbial biomass, especially Gram positive bacteria (cy19:0), Fungi(18:2ω6c) and Methane oxidizing bacteria(18:1ω8t). The rate of normal saturated fatty acid and monounsaturated fatty acid increased, and significantly increased after the interference of fire(p(pα-D-Lactose=2.87 p=0.080, FL-Threonine=3.00 p=0.078), and all of Tween 80, D-Mannitol, D-glucosaminic Acid were significantly difference by utilization of soil fungi(FTween 80=2.75 p=0.088, FD-Mannitol =3.53 p=0.047, FD-glucosaminic Acid=4.67 p=0.022),but the functional diversity of microbial community had not been effected by both the type of marsh and fire disturbance. [Conclusion]soil microbial biomass was correlated with the development stage of the marsh, and the soil microbial community structure was changed with the development stage of the marsh and fire disturbance. Soil bacteria and fungi were selective in the use of carbon resource.
- PLFA profiles,
- BIOLOG Eco-Plate,
- community level physiological profiling,
- soil property,
- discriminant function analysis

References

[1]	Haase J, Brandl R, Scheu S, et al. Above and belowground interactions are mediated by nutrient availability[J]. Ecology, 2008, 89:3072-3081.
[2]	Bartelt-Ryser J, Joshi J, Schmid B, et al. Soil feedbacks of plant diversity on soil microbial communities and subsequent plant growth[J]. Plant Ecology, Evolution and Systematics, 2005,7: 27-49.
[3]	Thoms C, Gattinge A, Jacob M, et al. Direct and indirect effects of tree diversity drive soil microbial diversity in temperate deciduous forest[J]. Soil Biology & Biochemistry,2010,42:1558-1565.
[4]	Fierer N, Strickland M S, Liptzin D, et al. Global patterns in belowground communities[J]. Ecology Letters, 2009, 12: 1238-1249.
[5]	Merila P, Malmivaara-Lamsa M, Spetz P, et al. Soil organic matter quality as a link between microbial community structure and vegetation composition along a successional gradient in a boreal forest[J]. Applied Soil Ecology, 2010, 46: 259-267.
[6]	Cardinale BJ, Duffy JE, Gonzalez A, et al. Biodiversity loss and its impact on humanity[J]. Nature, 2012, 486: 59-67.
[7]	Bardgett R D, van der Putten W H. Belowground biodiversity and ecosystem functioning[J]. Nature, 2014, 515: 505-511.
[8]	Tscherkoa D, Hammesfahr U, Zeltner G, et al. Plant succession and rhizophere microbial communities in recently deglaciated alpine terrain[J]. Basic and Applied Ecology, 2005,6:367-383.
[9]	Andersen R, Grasset L, Thormann M N, et al. Changes in microbial community structure and function following Sphagnum peatland restoration[J]. Soil Biology & Biochemistry, 2010, 42,291-301.
[10]	Vázquez F J, Acea M J, Carballas T. Soil microbial populations after wildfire[J]. FEMS Microbiology Ecology, 1993,13: 93-103.
[11]	Fontúrbel MT, Barreiro A, Vega J A, et al. Effects of an experimental fire and post-fire stabilization treatments on soil microbial communities[J]. Geoderma, 2012,191:51-66.
[12]	白爱芹,傅伯杰,曲来叶,等,大兴安岭火烧迹地恢复初期土壤微生物群落特征[J].生态学报,2012,32(15): 4762-4771.
[13]	郑琼,崔晓阳,邸雪颖,等.不同林火强度对大兴安岭偃松林土壤微生物功能多样性的影响[J].林业科学,2012,48(5)95-100.
[14]	中国湿地植被编写委员会.《中国湿地植被》[S].北京:科学出版社1999.
[15]	Frostegård A, Bååth E, Tunlid A. Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty-acid analysis[J]. Soil Biology & Biochemistry, 1993, 25: 723-730.
[16]	Bååth E. The use of neutral lipid fatty acids to indicate the physiological conditions of soil fungi[J]. Microbial Ecology, 2003, 45: 373-383.
[17]	Frostegård Å, Tunlid A, Bååth E. Use and misuse of PLFA measurements in soils[J]. Soil Biology & Biochemistry,2011,43, 1621-1625.
[18]	Balser TC, Wixon D. Investigating biological control over soil carbon temperature sensitivity[J]. Global Change Biology, 2009,15:2935-2949.
[19]	甘秋妹,孙海龙,郑红,等.大兴安岭不同退化阶段土壤和植物C、N、P浓度及其化学计量特征[J].森林工程,2013,29(3): 1-5.
[20]	刘银良,闫敏华,孟宪明, 等.大兴安岭森林火灾对沼泽土壤的影响[J].地理科学,1995,15(4):378-384.
[21]	Joergensen R G, Wichern F. Quantitative assessment of the fungal contribution to microbial tissue in soil[J]. Soil Biology & Biochemistry, 2008, 40: 2977-2991.
[22]	Carrasco L, Gattinger A, Flieβbach A, et al. Estimation by PLFA of microbial community structure associated with the rhizosphere of Lygeum spartum and Piptatherwn miliaceum growing in semiarid mine tailings[J]. Microbial Ecology, 2010,60: 265-271.
[23]	Fang J, Barcelona M J, Alvarez P J. A direct comparison between fatty acid analysis and intact phospholipid profiling for microbial identification[J]. Soil Biology and Biochemistry, 2000, 31:881-887.
[24]	Thiet R K, Frey S D, Six J. Do growth yield efficiencies differ between soil microbial community differing in fungal: bacterial ratios? Reality check and methodological issue[J]. Soil Biology and Biochemistry, 2006,38: 837-844.
[25]	Vries de F T, Hoffland E, Van Eekeren N, et al. Fungal/bacterial ratios in grasslands with contrasting nitrogen management[J]. Soil Biology and Biochemistry, 2006, 38:2092-2103.
[26]	Diedhiou S,Dossa E L,Badiane A N,et al.Decomposition and spatial microbial heterogeneity associated with native shrubs in soils of agroecosystems in semi-arid Senegal[J]. Pedobiologia, 2009,52: 273-286.
[27]	张莉,党军,刘伟,等.高寒草甸连续围封与施肥对土壤微生物群落结构的影响[J].应用生态学报,2012,23 (11):3072-3078.
[28]	Marschner P, Kandeler E, Marschner B. Structure and function of the soil microbial community in a long-term fertilizer experiment[J]. Soil Biology and Biochemistry, 2003, 35: 453-461.
[29]	Opelt K , Berg C, Schönmann S, Eberl L, Berg G. High specificity but contrasting biodiversity of Sphagnum-associated bacterial and plant communities in bog ecosystems independent of the geographical region[J]. The ISME Journal, 2007, 1, 502-516.
[30]	Preston M D, Smemo K A, McLaughlin J W, et al. Peatland microbial communities and decomposition processes in the James Bay Lowlands, Canada[J]. Frontiers in Microbiology, 2012,3,1-15.
[31]	Pratt B, Riesen R,Johnston C G. PLFA Analyses of Microbial Communities Associated with PAH-Contaminated Riverbank Sediment[J]. Microbial Ecology, 2012,64:680-691.
[32]	丁维新,蔡祖聪.土壤甲烷氧化菌及水分状况对其活性的影响[J].中国生态农业学报, 2003,11(1):94-97.
[33]	倪永清,史学伟,郑晓吉,等.冻土甲烷循环微生物群落及其对全球变化的响应[J].生态学报,2011,31(13):3846-3855.
[34]	Hebel C L, Smith J E, Jr Cromack K. Invasive plant species and soil microbial response to wildfire burn severity in the Cascade Range of Oregon[J]. Applied Soil Ecology, 2009, 42:150-159.
[35]	Wang M, Qu L Y, Ma K M, et al. Soil microbial properties under different vegetation types on Mountain Han[J]. Science China Life Sciences, 2013, 56: 561-570.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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The Effect of Forest Marsh and Fire Disturbance on Soil Microbial in Greater Xing'an Mountain

1. Institute of Wetland Research, Chinese Academy of Forestry, Beijing 100091

2. Institute of Heilongjiang Provincial Monitoring and Planning of Forestry, Harbin 154080, Heilongjiang

3. The Administration Bureau of Jiuwan Mountain National Natural Reserve, Liuzhou 545300, Guangxi

4. Research Institute of Agriculture and Forestry, Greater xing'an Forestry Group, Jiagedaqi 165000, Heilongjiang

Received Date: 2015-06-03

Abstract: [Objective]The aim of this paper was studied the characteristic of soil microbiological community and its diversity at forest marsh development and fire disturbance, to understand the role of soil microbial community in the forest marsh conservation and restoration.[Methods]We used phospholipid fatty acids (PLFAs) to portrait the community composition and community level physiological profiles (CLPP) to describe the functional diversity of the microbial community. [Results]Total of 5 types of marsh were chosen in Natural reserve area of Nanwenhe in Greater xing'an Mountain, which included xing'an Larch-Ledum palustre-moss marsh, xing'an Larch-xing'an azalea-moss marsh, xing'an Larch-birch-carex marsh, and Larch-xing'an azalea-moss marsh by severe fire disturbance, xing'an Larch-birch-carex marsh by mild fire disturbance at 2006. It was found that the PLFAs of 16:00(16.29±5.62 nmol·g-1), 18:1ω8t(9.89±8.61 nmol·g-1)and 16:1ω7c(9.79±3.24 nmol·g-1)were dominance in microbial community, and the dominant species contributed significantly to variations in soil microbial biomass, especially Gram positive bacteria (cy19:0), Fungi(18:2ω6c) and Methane oxidizing bacteria(18:1ω8t). The rate of normal saturated fatty acid and monounsaturated fatty acid increased, and significantly increased after the interference of fire(p(pα-D-Lactose=2.87 p=0.080, FL-Threonine=3.00 p=0.078), and all of Tween 80, D-Mannitol, D-glucosaminic Acid were significantly difference by utilization of soil fungi(FTween 80=2.75 p=0.088, FD-Mannitol =3.53 p=0.047, FD-glucosaminic Acid=4.67 p=0.022),but the functional diversity of microbial community had not been effected by both the type of marsh and fire disturbance. [Conclusion]soil microbial biomass was correlated with the development stage of the marsh, and the soil microbial community structure was changed with the development stage of the marsh and fire disturbance. Soil bacteria and fungi were selective in the use of carbon resource.

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Reference (35)

[1]	Haase J, Brandl R, Scheu S, et al. Above and belowground interactions are mediated by nutrient availability[J]. Ecology, 2008, 89:3072-3081.
[2]	Bartelt-Ryser J, Joshi J, Schmid B, et al. Soil feedbacks of plant diversity on soil microbial communities and subsequent plant growth[J]. Plant Ecology, Evolution and Systematics, 2005,7: 27-49.
[3]	Thoms C, Gattinge A, Jacob M, et al. Direct and indirect effects of tree diversity drive soil microbial diversity in temperate deciduous forest[J]. Soil Biology & Biochemistry,2010,42:1558-1565.
[4]	Fierer N, Strickland M S, Liptzin D, et al. Global patterns in belowground communities[J]. Ecology Letters, 2009, 12: 1238-1249.
[5]	Merila P, Malmivaara-Lamsa M, Spetz P, et al. Soil organic matter quality as a link between microbial community structure and vegetation composition along a successional gradient in a boreal forest[J]. Applied Soil Ecology, 2010, 46: 259-267.
[6]	Cardinale BJ, Duffy JE, Gonzalez A, et al. Biodiversity loss and its impact on humanity[J]. Nature, 2012, 486: 59-67.
[7]	Bardgett R D, van der Putten W H. Belowground biodiversity and ecosystem functioning[J]. Nature, 2014, 515: 505-511.
[8]	Tscherkoa D, Hammesfahr U, Zeltner G, et al. Plant succession and rhizophere microbial communities in recently deglaciated alpine terrain[J]. Basic and Applied Ecology, 2005,6:367-383.
[9]	Andersen R, Grasset L, Thormann M N, et al. Changes in microbial community structure and function following Sphagnum peatland restoration[J]. Soil Biology & Biochemistry, 2010, 42,291-301.
[10]	Vázquez F J, Acea M J, Carballas T. Soil microbial populations after wildfire[J]. FEMS Microbiology Ecology, 1993,13: 93-103.
[11]	Fontúrbel MT, Barreiro A, Vega J A, et al. Effects of an experimental fire and post-fire stabilization treatments on soil microbial communities[J]. Geoderma, 2012,191:51-66.
[12]	白爱芹,傅伯杰,曲来叶,等,大兴安岭火烧迹地恢复初期土壤微生物群落特征[J].生态学报,2012,32(15): 4762-4771.
[13]	郑琼,崔晓阳,邸雪颖,等.不同林火强度对大兴安岭偃松林土壤微生物功能多样性的影响[J].林业科学,2012,48(5)95-100.
[14]	中国湿地植被编写委员会.《中国湿地植被》[S].北京:科学出版社1999.
[15]	Frostegård A, Bååth E, Tunlid A. Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty-acid analysis[J]. Soil Biology & Biochemistry, 1993, 25: 723-730.
[16]	Bååth E. The use of neutral lipid fatty acids to indicate the physiological conditions of soil fungi[J]. Microbial Ecology, 2003, 45: 373-383.
[17]	Frostegård Å, Tunlid A, Bååth E. Use and misuse of PLFA measurements in soils[J]. Soil Biology & Biochemistry,2011,43, 1621-1625.
[18]	Balser TC, Wixon D. Investigating biological control over soil carbon temperature sensitivity[J]. Global Change Biology, 2009,15:2935-2949.
[19]	甘秋妹,孙海龙,郑红,等.大兴安岭不同退化阶段土壤和植物C、N、P浓度及其化学计量特征[J].森林工程,2013,29(3): 1-5.
[20]	刘银良,闫敏华,孟宪明, 等.大兴安岭森林火灾对沼泽土壤的影响[J].地理科学,1995,15(4):378-384.
[21]	Joergensen R G, Wichern F. Quantitative assessment of the fungal contribution to microbial tissue in soil[J]. Soil Biology & Biochemistry, 2008, 40: 2977-2991.
[22]	Carrasco L, Gattinger A, Flieβbach A, et al. Estimation by PLFA of microbial community structure associated with the rhizosphere of Lygeum spartum and Piptatherwn miliaceum growing in semiarid mine tailings[J]. Microbial Ecology, 2010,60: 265-271.
[23]	Fang J, Barcelona M J, Alvarez P J. A direct comparison between fatty acid analysis and intact phospholipid profiling for microbial identification[J]. Soil Biology and Biochemistry, 2000, 31:881-887.
[24]	Thiet R K, Frey S D, Six J. Do growth yield efficiencies differ between soil microbial community differing in fungal: bacterial ratios? Reality check and methodological issue[J]. Soil Biology and Biochemistry, 2006,38: 837-844.
[25]	Vries de F T, Hoffland E, Van Eekeren N, et al. Fungal/bacterial ratios in grasslands with contrasting nitrogen management[J]. Soil Biology and Biochemistry, 2006, 38:2092-2103.
[26]	Diedhiou S,Dossa E L,Badiane A N,et al.Decomposition and spatial microbial heterogeneity associated with native shrubs in soils of agroecosystems in semi-arid Senegal[J]. Pedobiologia, 2009,52: 273-286.
[27]	张莉,党军,刘伟,等.高寒草甸连续围封与施肥对土壤微生物群落结构的影响[J].应用生态学报,2012,23 (11):3072-3078.
[28]	Marschner P, Kandeler E, Marschner B. Structure and function of the soil microbial community in a long-term fertilizer experiment[J]. Soil Biology and Biochemistry, 2003, 35: 453-461.
[29]	Opelt K , Berg C, Schönmann S, Eberl L, Berg G. High specificity but contrasting biodiversity of Sphagnum-associated bacterial and plant communities in bog ecosystems independent of the geographical region[J]. The ISME Journal, 2007, 1, 502-516.
[30]	Preston M D, Smemo K A, McLaughlin J W, et al. Peatland microbial communities and decomposition processes in the James Bay Lowlands, Canada[J]. Frontiers in Microbiology, 2012,3,1-15.
[31]	Pratt B, Riesen R,Johnston C G. PLFA Analyses of Microbial Communities Associated with PAH-Contaminated Riverbank Sediment[J]. Microbial Ecology, 2012,64:680-691.
[32]	丁维新,蔡祖聪.土壤甲烷氧化菌及水分状况对其活性的影响[J].中国生态农业学报, 2003,11(1):94-97.
[33]	倪永清,史学伟,郑晓吉,等.冻土甲烷循环微生物群落及其对全球变化的响应[J].生态学报,2011,31(13):3846-3855.
[34]	Hebel C L, Smith J E, Jr Cromack K. Invasive plant species and soil microbial response to wildfire burn severity in the Cascade Range of Oregon[J]. Applied Soil Ecology, 2009, 42:150-159.
[35]	Wang M, Qu L Y, Ma K M, et al. Soil microbial properties under different vegetation types on Mountain Han[J]. Science China Life Sciences, 2013, 56: 561-570.

The Effect of Forest Marsh and Fire Disturbance on Soil Microbial in Greater Xing'an Mountain

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通讯作者: 陈斌, bchen63@163.com

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The Effect of Forest Marsh and Fire Disturbance on Soil Microbial in Greater Xing'an Mountain

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