Physiological Responses of Rhus chinensis under Lead Stress

SHI Xiang; WANG Shu-feng; PAN Hong-wei; SUN Hai-jing; CHEN Yi-tai; JIANG Ze-ping

Volume 29 Issue 1

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Physiological Responses of Rhus chinensis under Lead Stress

1.
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding of Zhejiang Province, Hangzhou 311400, Zhejiang, China
2.
Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Received Date: 2015-04-09

Abstract

[Objective]A greenhouse pot experiment was conducted to evaluate the potential of Rhus chinensis Mill for phytoremediation of lead-contaminated soil.[Method]The response of container seedlings to Pb concentrations (0, 400 and 1 000 mg·kg-1) in the soil was studied. Seedling growth, chlorophyll, carotenoid, chlorophyll fluorescence, malondialdehyde (MDA), nutrient elements, Pb accumulation and translocation were assessed.[Result]The results showed that R. chinensis did not show visual symptoms of Pb toxicity. Compared to the control, the biomass increased slightly at low concentration (400 mg·kg-1). Under Pb stress conditions, the root elongation was restrained, while the radial growth of root was promoted and the fine root was developed better. No differences were observed in photosynthetic pigments, chlorophyll fluorescence, and MDA. During the experiment, only small quantity of Pb were uptake by R. chinensis, and most of the Pb absorbed by plants was retained in the roots. However, at the high concentration (1 000 mg·kg-1), R. chinensis transported metal to the shoots better, and the translocation factor (TF) value was 0.66. Under the Pb stress, oxalic acid of root exudates was increased significantly; while the root could be induced malic acid and citric acid, and the concentration increased with Pb concentration in soil. [Conclusion]R. chinensis was found to have Pb tolerance and phytoremediation potential in Pb-contaminated soils.
- lead,
- Rhus chinensis,
- physiological response,
- root morphology,
- root exudate

References

[1]	Soodan R K, Pakade Y B, Nagpal A, et al. Analytical techniques for estimation of heavy metals in soil ecosystem: A tabulated review[J]. Talanta, 2014, 125: 405-410.
[2]	Bolan N, Kunhikrishnan A, Thangarajan R, et al. Remediation of heavy metal(loid)s contaminated soils; To mobilize or to immobilize?[J]. Journal of Hazardous Materials, 2014, 266:141-166.
[3]	Sharma P, Dubey R S. Lead toxicity in plants[J]. Brazilian Journal of Plant Physiology, 2005, 17(1): 35-52.
[4]	段德超, 于明革, 施积炎. 植物对铅的吸收、转运、累积和解毒机制研究进展[J]. 应用生态学报, 2014, 25(1) : 287-296.
[5]	Uzu G, Sobanska S, Aliouane Y, et al. Study of lead phytoavailability for atmospheric industrial micronic and sub-micronic particles in relation with lead speciation[J]. Environmental Pollution, 2009, 157(4): 1178-1185.
[6]	Punamiya P, Datta R, Sarkar D, et al. Symbiotic role of Glomus mosseae in phytoextraction of lead in vetiver grass[Chrysopogon zizanioides (L.)][J]. Journal of Hazardous Materials, 2010, 177(1-3): 465-474.
[7]	Wang H H, Shan X Q, Wen B, et al. Effect of indole-3-acetic acid on lead accumulation in maize (Zea mays L.) seedlings and the relevant antioxidant response[J]. Environmental and Experimental Botany, 2007, 61(3): 246-253.
[8]	Wojas S, Ruszczyńska A, Bulska E, et al. Ca²⁺-dependent plant response to Pb²⁺ is regulated by LCT1[J]. Environmental Pollution, 2007, 147(3): 584-592.
[9]	Sahi S V, Bryant N L, Sharma N C, et al. Characterization of a lead hyperaccumulator shrub, Sesbania drummondii[J]. Environmental Science & Technology, 2002, 36(21): 4676-4680.
[10]	Tian S K, Lu L L, Yang X E, et al. Spatial imaging and speciation of lead in the accumulator plant Sedum alfredii by microscopically focused synchrotron X-ray investigation[J]. Environmental Science & Technology, 2010, 44(15): 5920-5926.
[11]	Meyers D E R, Auchterlonie G J, Webb R I, et al. Uptake and localisation of lead in the root system of Brassica juncea[J]. Environmental Pollution, 2008, 153(2): 323-332.
[12]	Kopittke P M, Asher C J, Kopittke R A, et al. Toxic effects of Pb²⁺ on growth of cowpea (Vigna unguiculata)[J]. Environmental Pollution, 2007, 150(2): 280-287.
[13]	Tang Y T, Qiu R L, Zeng X W, et al. Lead, zinc cadmium accumulation and growth simulation in Arabis paniculata Franch[J]. Environmental and Experimental Botany, 2009, 66(1): 126-134.
[14]	Strycharz S, Newman L. Use of native plants for remediation of trichloroethylene: I. Deciduous trees[J]. International Journal of Phytoremediation, 2009, 11(2): 150-170.
[15]	Baccioa D D, Castagna A, Tognetti R, et al. Early responses to cadmium of two poplar clones that differ in stress tolerance[J]. Journal of Plant Physiology, 2014, 171(18): 1693-1705.
[16]	Evlard A, Sergeant K, Printz B, et al. A multiple-level study of metal tolerance in Salix fragilis and Salix aurita clones[J]. Journal of Proteomics, 2014, 101: 113-129.
[17]	de Souza S C R, de Andrade S A L, de Souza L A, et al. Lead tolerance and phytoremediation potential of Brazilian leguminous tree species at the seedling stage[J]. Journal of Environmental Management, 2012, 110: 299-307.
[18]	Rascio N, Navari-Izzo F. Heavy metal hyperaccumulating plants: How and why do they do it? And whatmakes them so interesting?[J]. Plant Science, 2011, 180(2): 169-181.
[19]	Bhargava A, Carmona F F, Bhargava M, et al. Approaches for enhanced phytoextraction of heavy metals[J]. Journal of Environmental Management, 2012, 105: 103-120.
[20]	Shu W S, Ye Z H, Zhang Z Q, et al. Natural colonization of plants on five lead/zinc mine tailings in southern China[J]. Restoration Ecology, 2005, 13(1): 49-60.
[21]	Lowther J R. Use of a single sulphuric acid-hydrogen peroxide digest for the analysis of Pinus radiata needles[J]. Communications in Soil Science and Plant Analysis, 1980, 11(2): 175-188.
[22]	Mukherjee S K, Asanuma S. Possible role of cellular phosphate pool and subsequent accumulation of inorganic phosphate on the aluminum tolerance in Bradyrhizobium japonicum[J]. Soil Biology & Biochemistry, 1998, 30(12): 1511-1156.
[23]	鲍士旦. 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2000: 46-109
[24]	Lichtenthaler F W, Cuny E, Weprek S. Eine einfache und leistungsfähige synthese acylierter glyculosylbromide aus hydroxyglycal-estern[J]. Angewandte Chemie, 1983, 95(11): 906-908
[25]	Aravind P, Prasad M N V. Zinc alleviates cadmium-induced oxidative stress in Ceratophyllum demersum L.: a free floating freshwater macrophyte[J]. Plant Physiology and Biochemistry, 2003, 41(4): 391-397.
[26]	乔冬梅. 基于黑麦草根系分泌有机酸的铅污染修复机理研究[D]. 北京: 中国农业科学院, 2010.
[27]	Pérez-Esteban J, Escolástico C, Ruiz-Fernández J, et al. Bioavailability and extraction of heavy metals from contaminated soil by Atriplex halimus[J]. Environmental and Experimental Botany, 2013, 88: 53-59.
[28]	Pottier M, García de la Torre VS, Victor C, et al. Genotypic variations in the dynamics of metal concentrations in poplar leaves: A field study with a perspective on phytoremediation[J]. Environmental Pollution, 2015, 199: 72-83.
[29]	Keller C, Hammer D, Kayser A, et al. Root development and heavy metal phytoextraction efficiency: comparison of different plant species in the field[J]. Plant and Soil, 2003, 249(1): 67-81.
[30]	王树凤. 柳树对重金属铅、镉响应的基因型差异及其耐性机制研究[D]. 杭州: 浙江大学, 2015.
[31]	Wang S F, Shi X, Sun H J, et al. Variations in metal tolerance and accumulation in three hydroponically cultivated varieties of Salix integra treated with lead[J]. PLoS One, 2014, 9(9): e108568
[32]	Kumar A, Prasad M N V. Lead-induced toxicity and interference in chlorophyll fluorescence in Talinum triangulare grown hydroponically[J]. Photosynthetica, 2015, 53 (1): 66-71.
[33]	胡筑兵, 陈亚华, 王桂萍, 等. 铜胁迫对玉米幼苗生长、叶绿素荧光参数和抗氧化酶[J]. 植物学通报, 2006, 23(2): 129-137.
[34]	万雪琴, 张帆, 夏新莉, 等. 镉处理对杨树光合作用及叶绿素荧光参数的影响[J]. 林业科学, 2008, 44(6): 73-78.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Physiological Responses of Rhus chinensis under Lead Stress

1. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding of Zhejiang Province, Hangzhou 311400, Zhejiang, China

2. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

Received Date: 2015-04-09

Abstract: [Objective]A greenhouse pot experiment was conducted to evaluate the potential of Rhus chinensis Mill for phytoremediation of lead-contaminated soil.[Method]The response of container seedlings to Pb concentrations (0, 400 and 1 000 mg·kg-1) in the soil was studied. Seedling growth, chlorophyll, carotenoid, chlorophyll fluorescence, malondialdehyde (MDA), nutrient elements, Pb accumulation and translocation were assessed.[Result]The results showed that R. chinensis did not show visual symptoms of Pb toxicity. Compared to the control, the biomass increased slightly at low concentration (400 mg·kg-1). Under Pb stress conditions, the root elongation was restrained, while the radial growth of root was promoted and the fine root was developed better. No differences were observed in photosynthetic pigments, chlorophyll fluorescence, and MDA. During the experiment, only small quantity of Pb were uptake by R. chinensis, and most of the Pb absorbed by plants was retained in the roots. However, at the high concentration (1 000 mg·kg-1), R. chinensis transported metal to the shoots better, and the translocation factor (TF) value was 0.66. Under the Pb stress, oxalic acid of root exudates was increased significantly; while the root could be induced malic acid and citric acid, and the concentration increased with Pb concentration in soil. [Conclusion]R. chinensis was found to have Pb tolerance and phytoremediation potential in Pb-contaminated soils.

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Reference (34)

[1]	Soodan R K, Pakade Y B, Nagpal A, et al. Analytical techniques for estimation of heavy metals in soil ecosystem: A tabulated review[J]. Talanta, 2014, 125: 405-410.
[2]	Bolan N, Kunhikrishnan A, Thangarajan R, et al. Remediation of heavy metal(loid)s contaminated soils; To mobilize or to immobilize?[J]. Journal of Hazardous Materials, 2014, 266:141-166.
[3]	Sharma P, Dubey R S. Lead toxicity in plants[J]. Brazilian Journal of Plant Physiology, 2005, 17(1): 35-52.
[4]	段德超, 于明革, 施积炎. 植物对铅的吸收、转运、累积和解毒机制研究进展[J]. 应用生态学报, 2014, 25(1) : 287-296.
[5]	Uzu G, Sobanska S, Aliouane Y, et al. Study of lead phytoavailability for atmospheric industrial micronic and sub-micronic particles in relation with lead speciation[J]. Environmental Pollution, 2009, 157(4): 1178-1185.
[6]	Punamiya P, Datta R, Sarkar D, et al. Symbiotic role of Glomus mosseae in phytoextraction of lead in vetiver grass[Chrysopogon zizanioides (L.)][J]. Journal of Hazardous Materials, 2010, 177(1-3): 465-474.
[7]	Wang H H, Shan X Q, Wen B, et al. Effect of indole-3-acetic acid on lead accumulation in maize (Zea mays L.) seedlings and the relevant antioxidant response[J]. Environmental and Experimental Botany, 2007, 61(3): 246-253.
[8]	Wojas S, Ruszczyńska A, Bulska E, et al. Ca²⁺-dependent plant response to Pb²⁺ is regulated by LCT1[J]. Environmental Pollution, 2007, 147(3): 584-592.
[9]	Sahi S V, Bryant N L, Sharma N C, et al. Characterization of a lead hyperaccumulator shrub, Sesbania drummondii[J]. Environmental Science & Technology, 2002, 36(21): 4676-4680.
[10]	Tian S K, Lu L L, Yang X E, et al. Spatial imaging and speciation of lead in the accumulator plant Sedum alfredii by microscopically focused synchrotron X-ray investigation[J]. Environmental Science & Technology, 2010, 44(15): 5920-5926.
[11]	Meyers D E R, Auchterlonie G J, Webb R I, et al. Uptake and localisation of lead in the root system of Brassica juncea[J]. Environmental Pollution, 2008, 153(2): 323-332.
[12]	Kopittke P M, Asher C J, Kopittke R A, et al. Toxic effects of Pb²⁺ on growth of cowpea (Vigna unguiculata)[J]. Environmental Pollution, 2007, 150(2): 280-287.
[13]	Tang Y T, Qiu R L, Zeng X W, et al. Lead, zinc cadmium accumulation and growth simulation in Arabis paniculata Franch[J]. Environmental and Experimental Botany, 2009, 66(1): 126-134.
[14]	Strycharz S, Newman L. Use of native plants for remediation of trichloroethylene: I. Deciduous trees[J]. International Journal of Phytoremediation, 2009, 11(2): 150-170.
[15]	Baccioa D D, Castagna A, Tognetti R, et al. Early responses to cadmium of two poplar clones that differ in stress tolerance[J]. Journal of Plant Physiology, 2014, 171(18): 1693-1705.
[16]	Evlard A, Sergeant K, Printz B, et al. A multiple-level study of metal tolerance in Salix fragilis and Salix aurita clones[J]. Journal of Proteomics, 2014, 101: 113-129.
[17]	de Souza S C R, de Andrade S A L, de Souza L A, et al. Lead tolerance and phytoremediation potential of Brazilian leguminous tree species at the seedling stage[J]. Journal of Environmental Management, 2012, 110: 299-307.
[18]	Rascio N, Navari-Izzo F. Heavy metal hyperaccumulating plants: How and why do they do it? And whatmakes them so interesting?[J]. Plant Science, 2011, 180(2): 169-181.
[19]	Bhargava A, Carmona F F, Bhargava M, et al. Approaches for enhanced phytoextraction of heavy metals[J]. Journal of Environmental Management, 2012, 105: 103-120.
[20]	Shu W S, Ye Z H, Zhang Z Q, et al. Natural colonization of plants on five lead/zinc mine tailings in southern China[J]. Restoration Ecology, 2005, 13(1): 49-60.
[21]	Lowther J R. Use of a single sulphuric acid-hydrogen peroxide digest for the analysis of Pinus radiata needles[J]. Communications in Soil Science and Plant Analysis, 1980, 11(2): 175-188.
[22]	Mukherjee S K, Asanuma S. Possible role of cellular phosphate pool and subsequent accumulation of inorganic phosphate on the aluminum tolerance in Bradyrhizobium japonicum[J]. Soil Biology & Biochemistry, 1998, 30(12): 1511-1156.
[23]	鲍士旦. 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2000: 46-109
[24]	Lichtenthaler F W, Cuny E, Weprek S. Eine einfache und leistungsfähige synthese acylierter glyculosylbromide aus hydroxyglycal-estern[J]. Angewandte Chemie, 1983, 95(11): 906-908
[25]	Aravind P, Prasad M N V. Zinc alleviates cadmium-induced oxidative stress in Ceratophyllum demersum L.: a free floating freshwater macrophyte[J]. Plant Physiology and Biochemistry, 2003, 41(4): 391-397.
[26]	乔冬梅. 基于黑麦草根系分泌有机酸的铅污染修复机理研究[D]. 北京: 中国农业科学院, 2010.
[27]	Pérez-Esteban J, Escolástico C, Ruiz-Fernández J, et al. Bioavailability and extraction of heavy metals from contaminated soil by Atriplex halimus[J]. Environmental and Experimental Botany, 2013, 88: 53-59.
[28]	Pottier M, García de la Torre VS, Victor C, et al. Genotypic variations in the dynamics of metal concentrations in poplar leaves: A field study with a perspective on phytoremediation[J]. Environmental Pollution, 2015, 199: 72-83.
[29]	Keller C, Hammer D, Kayser A, et al. Root development and heavy metal phytoextraction efficiency: comparison of different plant species in the field[J]. Plant and Soil, 2003, 249(1): 67-81.
[30]	王树凤. 柳树对重金属铅、镉响应的基因型差异及其耐性机制研究[D]. 杭州: 浙江大学, 2015.
[31]	Wang S F, Shi X, Sun H J, et al. Variations in metal tolerance and accumulation in three hydroponically cultivated varieties of Salix integra treated with lead[J]. PLoS One, 2014, 9(9): e108568
[32]	Kumar A, Prasad M N V. Lead-induced toxicity and interference in chlorophyll fluorescence in Talinum triangulare grown hydroponically[J]. Photosynthetica, 2015, 53 (1): 66-71.
[33]	胡筑兵, 陈亚华, 王桂萍, 等. 铜胁迫对玉米幼苗生长、叶绿素荧光参数和抗氧化酶[J]. 植物学通报, 2006, 23(2): 129-137.
[34]	万雪琴, 张帆, 夏新莉, 等. 镉处理对杨树光合作用及叶绿素荧光参数的影响[J]. 林业科学, 2008, 44(6): 73-78.

Physiological Responses of Rhus chinensis under Lead Stress

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通讯作者: 陈斌, bchen63@163.com

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Physiological Responses of Rhus chinensis under Lead Stress

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