Pathogenicity of Aerospace Mutants of Paecilomyces lilacinus against Meloidogyne incognita

WANG Xi-zhuo; WANG Lai-fa; MENG Fan-li; GUO Min-wei; PIAO Chun-gen; WANG Yuan

[Objective] To select some highly pathogenic strains in the aerospace mutants of Paecilomyces lilacinus obtained from its original strain Sd carried by the Shenzhou-8 spacecraft as biological control agents against southern root-knot nematodes Meloidogyne incognita. [Method]Their pathogenicity against M. incognita was evaluated by comparing their hyphal parasitizing rates on nematode eggs, inhibiting effect of their fermentation broths on egg hatching and juveniles. Their activities of such enzymes as protease and chitinase were also investigated. Based on these results, three optimum mutants (Sd-m-9, Sd-m-16 and Sd-m-26) were adopted for pot bioassay against M. incognita on pepper seedlings. [Results]The mutants Sd-m-9, Sd-m-16 and Sd-m-26 possessed the most evident virulence against M. incognita than the original strain Sd. Analysis of the relationships between the activities of protease, chitinase of aerospace mutants and parasitic rates indicated that the parasitic rate was significantly and positively correlated with the activities of protease and chitinase. The pot experiment of pepper seedlings planted in autoclaved soil showed that strains Sd-m-9, Sd-m-16 and Sd-m-26 were more effective for controlling M. incognita, the root-knot index was reduced by 88.34%~89.70% as compared with that of the original strain Sd. [Conclusion]It is suggest that the aerospace mutants Sd-m-9, Sd-m-16 and Sd-m-26 are of application potential for biological control of M. incognita.

1. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry

Received Date: 2015-11-06

Abstract: [Objective] To select some highly pathogenic strains in the aerospace mutants of Paecilomyces lilacinus obtained from its original strain Sd carried by the Shenzhou-8 spacecraft as biological control agents against southern root-knot nematodes Meloidogyne incognita. [Method]Their pathogenicity against M. incognita was evaluated by comparing their hyphal parasitizing rates on nematode eggs, inhibiting effect of their fermentation broths on egg hatching and juveniles. Their activities of such enzymes as protease and chitinase were also investigated. Based on these results, three optimum mutants (Sd-m-9, Sd-m-16 and Sd-m-26) were adopted for pot bioassay against M. incognita on pepper seedlings. [Results]The mutants Sd-m-9, Sd-m-16 and Sd-m-26 possessed the most evident virulence against M. incognita than the original strain Sd. Analysis of the relationships between the activities of protease, chitinase of aerospace mutants and parasitic rates indicated that the parasitic rate was significantly and positively correlated with the activities of protease and chitinase. The pot experiment of pepper seedlings planted in autoclaved soil showed that strains Sd-m-9, Sd-m-16 and Sd-m-26 were more effective for controlling M. incognita, the root-knot index was reduced by 88.34%~89.70% as compared with that of the original strain Sd. [Conclusion]It is suggest that the aerospace mutants Sd-m-9, Sd-m-16 and Sd-m-26 are of application potential for biological control of M. incognita.

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Reference (17)

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Pathogenicity of Aerospace Mutants of Paecilomyces lilacinus against Meloidogyne incognita

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References

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通讯作者: 陈斌, bchen63@163.com

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