Relationship between Endogenous Hormone Content and Embryo Growth and the Seed Germination of Paris polyphylla var. yunnansensis

PU Mei; SUN Yong-yu; GAO Cheng-jie; LI Kun

[Objective]To study the variation of embryo length and endogenous hormone content in seed during stratification so as to understand the dormancy mechanism of Paris polyphylla var. yunnansensis seed. [Method]The enzyme linked immunosorbent assay (ELISA) was used to measure the hormone contents of seed in various stages. [Result]The results showed that during the process of seed germination, the abscisic acid (ABA) content reduced from 54.34 ng·g-1·FW to 32.16 ng·g-1·FW, both the gibberellin (GA) and zeatin riboside (ZR) contents increased markedly, the indoleacetic acid (IAA) content fluctuated significantly, but increased gradually as a whole. With the extension of stratification time, the ABA content in the dormant seed reduced, while the GA content and the ratio of GA/ABA continued to rise, but they were still significantly lower than that in the germinating seed (pp[Conclusion]The results suggest that reducing ABA content alone may not be enough to release the dormancy of P. polyphylla var. yunnansensis during seed germination, and the increasing of GA content and the GA/ABA ratio reaching to a certain threshold may be able to release seed dormancy and promote the development of embryo effectively. Due to the combination of the various hormones, the seed gradually entered the dormancy breaking state and germination state after 40 days' stratification.

1. Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, Yunnan, China

Received Date: 2015-11-09

Abstract: [Objective]To study the variation of embryo length and endogenous hormone content in seed during stratification so as to understand the dormancy mechanism of Paris polyphylla var. yunnansensis seed. [Method]The enzyme linked immunosorbent assay (ELISA) was used to measure the hormone contents of seed in various stages. [Result]The results showed that during the process of seed germination, the abscisic acid (ABA) content reduced from 54.34 ng·g-1·FW to 32.16 ng·g-1·FW, both the gibberellin (GA) and zeatin riboside (ZR) contents increased markedly, the indoleacetic acid (IAA) content fluctuated significantly, but increased gradually as a whole. With the extension of stratification time, the ABA content in the dormant seed reduced, while the GA content and the ratio of GA/ABA continued to rise, but they were still significantly lower than that in the germinating seed (pp[Conclusion]The results suggest that reducing ABA content alone may not be enough to release the dormancy of P. polyphylla var. yunnansensis during seed germination, and the increasing of GA content and the GA/ABA ratio reaching to a certain threshold may be able to release seed dormancy and promote the development of embryo effectively. Due to the combination of the various hormones, the seed gradually entered the dormancy breaking state and germination state after 40 days' stratification.

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Reference (18)

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Relationship between Endogenous Hormone Content and Embryo Growth and the Seed Germination of Paris polyphylla var. yunnansensis

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通讯作者: 陈斌, bchen63@163.com

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