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Citation:

Genetic Relationships among Paulownia elongata, Paulownia fortunei and Paulownia tomentosa Based on cpDNA rps16 Region Sequences

  • Received Date: 2015-05-23
  • [Objective] To explore the change of chloroplast genetic characteristics and laws of Paulownia elongata, P. fortunei and P. tomentosa and to discuss the genetic relationship among the three species.[Method] The genetic differences of the Chloroplast DNA rps16 region sequences of P. elongata, P. fortunei and P. tomentosa were analyzed by using sequencing method. The DNAs were extracted from each 15 individuals of P. elongata, P. fortunei and P. tomentosa, then were amplified with PCR to obtain specific fragment which were purified and sequenced. Quick sort were completed by the software Clustal X 2.0, multiple sequence were aligned by using software MEGA 4, and then the characteristics and the K2P genetic distance of the alignment sequence were also calculated.[Result] (1). The sequence lengths of rps16 region from P. elongata were measured by 916~933 bp; those from P. fortunei was calculated by 932 bp; and those from P. tomentosa were 916~918 bp after aligned. The length of rps16 sequence was 938 bp after aligned by the software Clustal X 2.0, and the average GC content was 34.31%. The amount of total variable loci detected from the aligned sequences was 10, accounting for 1.07% of the entire length of the sequence. The amount of variable loci (insertion-deletion site) was 9, accounting for 90% of the total variable loci, for 0.96% of the entire sequence. The amount of variable loci (substitution site) was 1, accounting for 10% of the total variable loci, for 0.11% of the entire sequence. (2). Total 10 variable loci were detected from the aligned sequences, of which 9(90%) sites were same between P. elongata and P. fortunei, while there was no same base between P. elongata and P. tomentosa.[Conclusion] According to the analysis on sequence characteristics and mutation sites of rps16 sequences from the three species of Genus Paulownia, in chloroplast genetic aspects, the results illustrated that there were much more similar plastid inheritances between P. elongata and P. fortunei than between P. elongata and P. tomentosa; indicating closer relationships between P. elongata and P. fortunei. From the above, P. elongata and P. fortunei could be inferred from the same maternal lineage.
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  • [1] 李芳东, 乔杰, 王保平, 等. 中国泡桐属种质资源图谱[M]. 北京:中国林业出版社, 2013:2-12.

    [2] 邱乾栋, 莫文娟, 王楠, 等. 白花泡桐材色优良单株的选择[J]. 林业科学研究. 2014, 27(2):277-283.

    [3] 王楠, 李芳东, 莫文娟, 等. 杂交泡桐"毛白33"与其回交一代的光合特性比较[J]. 东北林业大学学报, 2015, 43(6):51-56.

    [4] 王楠, 李芳东, 马跃, 等. 泡桐优良无性系"TF33" 干材表型性状杂种优势研究[J]. 林业科学研究, 2015, 28(3):402-408.

    [5]

    Schwarzbach A E, Donovan L A, Rieseberg L H. Transgressive character expression in a hybrid sunflower species[J]. American Journal of Botany, 2001, 88(2):270-277.
    [6]

    Rauscher J T, Doyle J J, Brown A H D. Internal transcribed spacer repeat-specific primers and the analysis of hybridization in the Glycine tomentella (Leguminosae) polyploid complex[J]. Molecular Ecology, 2002, 11(12):2691-2702.
    [7] 竺肇华. 泡桐属植物的分布中心及区系成分的探讨[J]. 林业科学, 1981, 19(3):271-280.

    [8] 熊金桥, 陈志远. 泡桐属的数量分类研究[J]. 植物研究, 1992, 12(2):185-188.

    [9] 卢龙斗, 谢龙旭, 杜启艳. 泡桐属七种植物的RAPD分析[J]. 广西植物, 2001, 21(4):335-338.

    [10] 马浩, 张冬梅, 李荣幸, 等. 泡桐属植物种类的RFLP分析[J]. 植物研究, 2001, 21(1):136-139.

    [11] 莫文娟, 傅建敏, 乔杰, 等. 泡桐属植物亲缘关系的ISSR分析[J]. 林业科学, 2013, 49(1):61-67.

    [12] 高婷. 利用DNA条形码技术鉴定药用双子叶植物[D]. 中国协和医科大学, 2010.

    [13] 任保青,陈之端. 植物DNA条形码技术[J]. 植物学报, 20l0, 45(1):1-12.

    [14] 杨燕, 费改顺, 贾正平, 等. 半夏人工栽培技术及分子标记技术研究新进展[J]. 中药材, 2010(2):312-317.

    [15] 陈仁芳,张泽,唐洲,等. 桑属ITS、TrnL-F、rps16序列与进化分析[J]. 中国农业科学, 2011, 44(8):1553-1561.

    [16] 张金梅, 王建秀, 夏涛, 等. 基于系统发育分析的DNA条形码技术在澄清芍药属牡丹组物种问题中的应用[J]. 中国科学:C辑, 2008, 38(12):1166-1176.

    [17] 侯鑫, 刘俊娥, 赵一之, 等. 基于ITS序列和trnL-F序列探讨小叶锦鸡儿, 中间锦鸡儿和柠条锦鸡儿的种间关系[J]. 植物分类学报, 2006, 44(2):126-134.

    [18]

    Wallander E, Albert V A. Phylogeny and classification of Oleaceae based on rps16 and trnL-F sequence data[J]. American Journal of Botany, 2000, 87(12):1827-1841.
    [19]

    Baker W J, Hedderson T A, Dransfield J. Molecular phylogenetics of subfamily Calamoideae (Palmae) based on nrDNA ITS and cpDNA rps16 intron sequence data[J]. Molecular phylogenetics and evolution, 2000, 14(2):195-217.
    [20]

    Robbrecht E, Manen J F. The major evolutionary lineages of the coffee family (Rubiaceae, angiosperms). Combined analysis (n DNA and cp DNA) to infer the position of Coptosapelta and Luculia, and super tree construction based on rbcL, rps16, trnL-trnF and atpB-rbcL data. A new classification in two subfamilies, Cinchonoideae and Rubioideae[J]. Systematics and Geography of Plants, 2006:85-145.
    [21] 李芳东, 袁德义, 莫文娟, 等. 白花泡桐种源遗传多样性的ISSR分析[J]. 中南林业科技大学学报, 2011, 31(7):1-7.

    [22] 莫文娟, 袁德义, 李芳东, 等. 白花泡桐种源的遗传多样性和遗传分化研究[J]. 植物研究, 2011, 31(5):585-591.

    [23]

    Thompson J D, Gibson T J, Plewniak F, et al. The CLUSTAL_X windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic acids research, 1997, 25(24):4876-4882.
    [24]

    Tamura K, Dudley J, Nei M, et al. MEGA4:molecular evolutionary genetics analysis (MEGA) software version 4.0.[J]. Molecular Biology and Evolution, 2007, 24:1596-1599.
    [25]

    Vinnersten A, Reeves G. Phylogenetic relationships within Colchicaceae[J]. American Journal of Botany, 2003, 90(10):1455-1462.
    [26] 袁长春, 何雪宝, 袁秋梅, 等. 绿绒蒿自然杂交种及其亲本cpDNA trnL-trnF 基因的遗传学分析[J]. 云南植物研究, 2007, 29(1):103-108.

    [27] 宋育红, 张君诚, 张杭颖. 长柄石杉不同地理居群叶绿体DNA trnL-trnF 序列变异与聚类分析[J]. 应用与环境生物学报, 2014, 20(003):443-448.

    [28]

    Qi W, Yang H, Xue Y, et al. Inheritance of chloroplast and mitochondrial DNA in Chinese fir (Cunninghamia lanceolata)[J]. Acta Botanica Sinica, 1998, 41(7):695-699.
    [29] 李宗然. 泡桐研究进展[M]. 北京:中国林业出版社, 1995:226-230.

    [30] 胡惠蓉, 陈志远. 泡桐属叶表皮毛状体扫描电镜观察[J]. 华中农业大学学报, 1996, 15(2):190-193

    [31] 梁作椭. 泡桐属植物CAT、AWYI、ADH同工酶研究[D]. 武汉:华中农业大学, 1996.

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Genetic Relationships among Paulownia elongata, Paulownia fortunei and Paulownia tomentosa Based on cpDNA rps16 Region Sequences

  • 1. Forestry Experiment Center of North China, Chinese Academy of Forestry, Beijing 102300, China
  • 2. Non-timber Forestry Research & Development Center, Chinese Academy of Forestry, Zhengzhou 450003, He'nan, China
  • 3. College of Biological Sciences and Technology, Beijing Forest University, Beijing 100083, China

Abstract: [Objective] To explore the change of chloroplast genetic characteristics and laws of Paulownia elongata, P. fortunei and P. tomentosa and to discuss the genetic relationship among the three species.[Method] The genetic differences of the Chloroplast DNA rps16 region sequences of P. elongata, P. fortunei and P. tomentosa were analyzed by using sequencing method. The DNAs were extracted from each 15 individuals of P. elongata, P. fortunei and P. tomentosa, then were amplified with PCR to obtain specific fragment which were purified and sequenced. Quick sort were completed by the software Clustal X 2.0, multiple sequence were aligned by using software MEGA 4, and then the characteristics and the K2P genetic distance of the alignment sequence were also calculated.[Result] (1). The sequence lengths of rps16 region from P. elongata were measured by 916~933 bp; those from P. fortunei was calculated by 932 bp; and those from P. tomentosa were 916~918 bp after aligned. The length of rps16 sequence was 938 bp after aligned by the software Clustal X 2.0, and the average GC content was 34.31%. The amount of total variable loci detected from the aligned sequences was 10, accounting for 1.07% of the entire length of the sequence. The amount of variable loci (insertion-deletion site) was 9, accounting for 90% of the total variable loci, for 0.96% of the entire sequence. The amount of variable loci (substitution site) was 1, accounting for 10% of the total variable loci, for 0.11% of the entire sequence. (2). Total 10 variable loci were detected from the aligned sequences, of which 9(90%) sites were same between P. elongata and P. fortunei, while there was no same base between P. elongata and P. tomentosa.[Conclusion] According to the analysis on sequence characteristics and mutation sites of rps16 sequences from the three species of Genus Paulownia, in chloroplast genetic aspects, the results illustrated that there were much more similar plastid inheritances between P. elongata and P. fortunei than between P. elongata and P. tomentosa; indicating closer relationships between P. elongata and P. fortunei. From the above, P. elongata and P. fortunei could be inferred from the same maternal lineage.

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