Transcriptome Analysis of Male White Wax Scale Pupae

YU Shu-hui; QI Qian; SUN Tao; WANG Xue-qing; YANG Pu; FENG Ying

Volume 29 Issue 3

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Transcriptome Analysis of Male White Wax Scale Pupae

1.
Research Institute of Resources Insects, Chinese Academy of Forestry, Key laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2016-01-18

Abstract

[Objective] To obtain the transcriptomic data and characterize the transcriptome of pupal stage of male white wax scale insect, Ericerus pela.[Method] The high-throughput sequencing was performed by Illumina HiSeq 2000, and bioinformatics analysis were carried out subsequently. Three heat shock protein genes (hsps) were selected for expression profile analysis cross different developmental stages using quantitative real-time PCR (qRT-PCR).[Result] The transcriptomic sequencing generated 63957 unigenes, 63272 open reading frame (ORF), and 9561simple sequence repeat (SSR) markers. The average length of the unigenes was 674 bp. A total of 14327 unigenes were annotated in different databases. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the putative function of many genes coincided the physiological characterization of pupal stage.[Conclusion] The data and analysis of the transcriptome could lay a foundation for further gene functional research and proteomic analysis of E. pela.
- Ericerus pela,
- pupae,
- transcriptome,
- unigene,
- heat shock protein

References

[1]	Yang P, Chen X M, Liu W W, et al. Transcriptome analysis of sexually dimorphic Chinese white wax scale insects reveals key differences in developmental programs and transcription factor expression[J]. Sci Rep, 2015, 5:8141.
[2]	Truman J W, Riddiford L M. The origins of insect metamorphosis[J]. Nature,1999, 401(6752):447-452.
[3]	White K P, Rifkin S A, Hurban P, et al. Microarray analysis of Drosophila development during metamorphosis[J]. Science, 1999, 286(5447):2179-2184.
[4]	Fu Q, Liu P C, Wang J X, et al. Proteomic identification of differentially expressed and phosphorylated proteins in epidermis involved in larval-pupal metamorphosis of Helicoverpa armigera[J]. BMC Genomics, 2009, 10:600.
[5]	Man'e-Padr'os D, Cruz J, Vilaplana L, et al. The hormonal pathway controlling cell death during metamorphosis in a hemimetabolous insect[J]. Dev Biol, 2010, 346(1):150-160.
[6]	Gu J, Huang L X, Gong Y J, et al. De novo characterization of transcriptome and gene expression dynamics in epidermis during the larval-pupal metamorphosis of common cutworm[J]. Insect Biochem Mol Biol, 2013, 43(9):794-808.
[7]	Soares M P, Barchuk A R, Simoes A C, et al. Genes involved in thoracic exoskeleton formation during the pupal-to-adult molt in a social insect model, Apis mellifera[J]. BMC Genomics, 2013, 14:576.
[8]	Yang P, Zhu J Y, Gong Z J, et al. Transcriptome analysis of the Chinese white wax scale Ericerus pela with focus on genes involved in wax biosynthesis[J]. PLoS One, 2012, 7(4):e35719.
[9]	Yu S H, Yang P, Sun T, et al. Transcriptomic and proteomic analyses on the supercooling ability and mining of antifreeze proteins of the Chinese white wax scale insect[J] Insect Sci, 2016, 1:1-8.
[10]	Altschul S F, Madden T L, Schäffer A A, et al. Gapped BLAST and PSI-BLAST:a new generation of protein database search programs[J]. Nucleic Acids Res, 1997,25(17):3389-3402.
[11]	Deng Y Y, Li J Q, Wu S F, et al. Integrated nr database in protein annotation system and its localization[J]. Computer Engineering, 2006, 32(5):71-74.
[12]	Apweiler R, Bairoch A, Wu C H, et al. UniProt:the universal protein knowledgebase[J]. Nucleic Acids Res, 2004, 32:115-119.
[13]	Ashburner M, Ball C A, Blake J A, et al. Gene ontology:tool for the unification of biology. The Gene Ontology Consortium[J]. Nat Genet, 2000, 25(1):25-29.
[14]	Tatusov R L, Galperin M Y, Natale D A, et al. The COG database:a tool for genome-scale analysis of protein functions and evolution[J]. Nucleic Acids Res, 2000, 28(1):33-36.
[15]	Kanehisa M, Goto S, Kawashima S, et al. The KEGG resource for deciphering the genome[J]. Nucleic Acids Res, 2004, 32:277-280.
[16]	Arimoto M, Yamagishi K, Wang J, et al. Molecular breeding of lignin-degrading brown-rot fungus Gloeophyllum trabeum by homologous expression of laccase gene[J]. AMB Express, 2015, 5(1):81.
[17]	Liu W W, Yang P, Chen X M, et al. Cloning and expression analysis of four heat shock protein genes in Ericerus pela (Homoptera:Coccidae)[J]. J Insect Sci, 2014, 14(142):1-9.
[18]	Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods, 2001, 25(4):402-408.
[19]	Angelini D R, Kaufman T C. Functional analyses in the milkweed bug Oncopeltus fasciatus (Hemiptera) support a role for Wnt signaling in body segmentation but not appendage development[J]. Dev Biol, 2005, 283(2):409-423.
[20]	Zhu J Y, Yang P, Zhang Z, et al. Transcriptomic immune response of Tenebrio molitor pupae to parasitization by Scleroderma guani[J]. PLoS One, 2013, 8(1):e54411.
[21]	Yang P, Chen X M. Protein profiles of Chinese white wax scale, Ericerus pela, at the male pupal stage by high-throughput proteomics[J]. Arch Insect Biochem Physiol, 2014, 87(4):214-233.
[22]	Caplan A J, Jackson S, Smith D. Hsp90 reaches new heights. Conference on the Hsp90 chaperone machine[J]. EMBO Rep, 2003, 4(2):126-130.
[23]	Mahroof R, Zhu K Y, Neven L, et al. Expression patterns of three heat shock protein 70 genes among developmental stages of the red flour beetle, Tribolium castaneum (Coleoptera:Tenebrionidae)[J]. Mol Integr Physiol, 2005, 141(2):247-256.
[24]	Tachibana S, Numata H, Goto S G. Gene expression of heat-shock proteins (Hsp23, Hsp70 and Hsp90) during and after larval diapauses in the blow fly Lucilia sericata[J]. J Insect Physiol, 2005, 51(6):641-647.
[25]	de Andrade A, Siviero F, Rezende-Teixeira P, et al. Molecular characterization of a putative heat shock protein cognate gene in Rhynchosciara Americana[J]. Chromosome Res, 2009, 17(7):935-945.
[26]	Zhang Q R, Denlinger D L. Molecular characterization of heat shock protein 90, 70 and 70 cognate cDNAs and their expression patterns during thermal stress and pupal diapause in the corn earworm[J]. J Insect Physiol, 2010, 56(2):138-150.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Transcriptome Analysis of Male White Wax Scale Pupae

1. Research Institute of Resources Insects, Chinese Academy of Forestry, Key laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2016-01-18

Abstract: [Objective] To obtain the transcriptomic data and characterize the transcriptome of pupal stage of male white wax scale insect, Ericerus pela.[Method] The high-throughput sequencing was performed by Illumina HiSeq 2000, and bioinformatics analysis were carried out subsequently. Three heat shock protein genes (hsps) were selected for expression profile analysis cross different developmental stages using quantitative real-time PCR (qRT-PCR).[Result] The transcriptomic sequencing generated 63957 unigenes, 63272 open reading frame (ORF), and 9561simple sequence repeat (SSR) markers. The average length of the unigenes was 674 bp. A total of 14327 unigenes were annotated in different databases. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the putative function of many genes coincided the physiological characterization of pupal stage.[Conclusion] The data and analysis of the transcriptome could lay a foundation for further gene functional research and proteomic analysis of E. pela.

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Reference (26)

[1]	Yang P, Chen X M, Liu W W, et al. Transcriptome analysis of sexually dimorphic Chinese white wax scale insects reveals key differences in developmental programs and transcription factor expression[J]. Sci Rep, 2015, 5:8141.
[2]	Truman J W, Riddiford L M. The origins of insect metamorphosis[J]. Nature,1999, 401(6752):447-452.
[3]	White K P, Rifkin S A, Hurban P, et al. Microarray analysis of Drosophila development during metamorphosis[J]. Science, 1999, 286(5447):2179-2184.
[4]	Fu Q, Liu P C, Wang J X, et al. Proteomic identification of differentially expressed and phosphorylated proteins in epidermis involved in larval-pupal metamorphosis of Helicoverpa armigera[J]. BMC Genomics, 2009, 10:600.
[5]	Man'e-Padr'os D, Cruz J, Vilaplana L, et al. The hormonal pathway controlling cell death during metamorphosis in a hemimetabolous insect[J]. Dev Biol, 2010, 346(1):150-160.
[6]	Gu J, Huang L X, Gong Y J, et al. De novo characterization of transcriptome and gene expression dynamics in epidermis during the larval-pupal metamorphosis of common cutworm[J]. Insect Biochem Mol Biol, 2013, 43(9):794-808.
[7]	Soares M P, Barchuk A R, Simoes A C, et al. Genes involved in thoracic exoskeleton formation during the pupal-to-adult molt in a social insect model, Apis mellifera[J]. BMC Genomics, 2013, 14:576.
[8]	Yang P, Zhu J Y, Gong Z J, et al. Transcriptome analysis of the Chinese white wax scale Ericerus pela with focus on genes involved in wax biosynthesis[J]. PLoS One, 2012, 7(4):e35719.
[9]	Yu S H, Yang P, Sun T, et al. Transcriptomic and proteomic analyses on the supercooling ability and mining of antifreeze proteins of the Chinese white wax scale insect[J] Insect Sci, 2016, 1:1-8.
[10]	Altschul S F, Madden T L, Schäffer A A, et al. Gapped BLAST and PSI-BLAST:a new generation of protein database search programs[J]. Nucleic Acids Res, 1997,25(17):3389-3402.
[11]	Deng Y Y, Li J Q, Wu S F, et al. Integrated nr database in protein annotation system and its localization[J]. Computer Engineering, 2006, 32(5):71-74.
[12]	Apweiler R, Bairoch A, Wu C H, et al. UniProt:the universal protein knowledgebase[J]. Nucleic Acids Res, 2004, 32:115-119.
[13]	Ashburner M, Ball C A, Blake J A, et al. Gene ontology:tool for the unification of biology. The Gene Ontology Consortium[J]. Nat Genet, 2000, 25(1):25-29.
[14]	Tatusov R L, Galperin M Y, Natale D A, et al. The COG database:a tool for genome-scale analysis of protein functions and evolution[J]. Nucleic Acids Res, 2000, 28(1):33-36.
[15]	Kanehisa M, Goto S, Kawashima S, et al. The KEGG resource for deciphering the genome[J]. Nucleic Acids Res, 2004, 32:277-280.
[16]	Arimoto M, Yamagishi K, Wang J, et al. Molecular breeding of lignin-degrading brown-rot fungus Gloeophyllum trabeum by homologous expression of laccase gene[J]. AMB Express, 2015, 5(1):81.
[17]	Liu W W, Yang P, Chen X M, et al. Cloning and expression analysis of four heat shock protein genes in Ericerus pela (Homoptera:Coccidae)[J]. J Insect Sci, 2014, 14(142):1-9.
[18]	Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method[J]. Methods, 2001, 25(4):402-408.
[19]	Angelini D R, Kaufman T C. Functional analyses in the milkweed bug Oncopeltus fasciatus (Hemiptera) support a role for Wnt signaling in body segmentation but not appendage development[J]. Dev Biol, 2005, 283(2):409-423.
[20]	Zhu J Y, Yang P, Zhang Z, et al. Transcriptomic immune response of Tenebrio molitor pupae to parasitization by Scleroderma guani[J]. PLoS One, 2013, 8(1):e54411.
[21]	Yang P, Chen X M. Protein profiles of Chinese white wax scale, Ericerus pela, at the male pupal stage by high-throughput proteomics[J]. Arch Insect Biochem Physiol, 2014, 87(4):214-233.
[22]	Caplan A J, Jackson S, Smith D. Hsp90 reaches new heights. Conference on the Hsp90 chaperone machine[J]. EMBO Rep, 2003, 4(2):126-130.
[23]	Mahroof R, Zhu K Y, Neven L, et al. Expression patterns of three heat shock protein 70 genes among developmental stages of the red flour beetle, Tribolium castaneum (Coleoptera:Tenebrionidae)[J]. Mol Integr Physiol, 2005, 141(2):247-256.
[24]	Tachibana S, Numata H, Goto S G. Gene expression of heat-shock proteins (Hsp23, Hsp70 and Hsp90) during and after larval diapauses in the blow fly Lucilia sericata[J]. J Insect Physiol, 2005, 51(6):641-647.
[25]	de Andrade A, Siviero F, Rezende-Teixeira P, et al. Molecular characterization of a putative heat shock protein cognate gene in Rhynchosciara Americana[J]. Chromosome Res, 2009, 17(7):935-945.
[26]	Zhang Q R, Denlinger D L. Molecular characterization of heat shock protein 90, 70 and 70 cognate cDNAs and their expression patterns during thermal stress and pupal diapause in the corn earworm[J]. J Insect Physiol, 2010, 56(2):138-150.

Transcriptome Analysis of Male White Wax Scale Pupae

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通讯作者: 陈斌, bchen63@163.com

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Transcriptome Analysis of Male White Wax Scale Pupae

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