Comparison in Contents of Polyphenol and Flavonoid in Leaves of Eucommia ulmoides Germplasm

WEI Yan-xiu; LIU Pan-feng; DU Qing-xin; DU Hong-yan

Volume 29 Issue 4

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Article Navigation > Forest Research > 2016 > 29(4): 529-535

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Comparison in Contents of Polyphenol and Flavonoid in Leaves of Eucommia ulmoides Germplasm

1.
Non-timber Forest Research and Development Center of Chinese Academy of Forestry, the Eucommia Engineering Research Center of State Forestry Administration, Zhengzhou 450003, He'nan, China

Received Date: 2015-12-08

Abstract

[Objective] To compare the content difference of polyphenol and flavonoid in Eucommia ulmoides leaves, so as to evaluate and utilize E. ulmoides germplasm reasonably. [Method] HPLC and spectrophotometric methods were used to measure the contents of polyphenol, total flavonoid, isoquercitrin and quercetin of 105 E. ulmoides germplasm. [Result] The mean content of quercetin was 0.33 mg·g-1 with the highest variation coefficient of 42.42%, while the mean content of total flavonoid was 15.92 mg·g-1 with the lowest variation coefficient of 19.35%; the mean content of isoquercitrin, total polyphenol was 3.37 mg·g-1 and 42.74 mg·g-1, respectively, with the variation coefficient of 34.42% and 23.72%. No significant correlation was found in the contents of these four components between male and female plant leaves. There was highly significant difference in the four components among different regions (PE. ulmoides germplasm resources could be divided into four groups according to the content difference of the four components, in which the contents of flavonoid compounds and polyphenol in group Ⅲ including 13 materials were higher than that of the other groups. [Conclusion] The contents of polyphenol and flavonoid in leaves of E. ulmoides were high and abundant diversity and variation were found in different germplasms, which showed great potential for selection and improvement. Thus, it could provide basis materials for breeding Eucommia ulmoides germplasm.
- Eucommia ulmoides leaves,
- active ingredients,
- polyphenol,
- flavonoid,
- comparison

References

[1]	杜红岩,胡文臻,俞锐.杜仲产业绿皮书[M].北京:社会科学文献出版社,2013.
[2]	杜红岩,赵戈.论我国杜仲产业化与培育技术的发展[J].林业科学研究,2000,13(5):554-561
[3]	黄海燕, 杜红岩, 乌云塔娜,等. 基于SSR分子标记的杜仲遗传多样性体系建立[J]. 林业科学研究, 2013, 26(6):795-799.
[4]	杜红岩. 杜仲活性成分与药理研究的新进展[J]. 经济林研究, 2004, 21(2):58-61.
[5]	王璐, 杜兰英, 杜红岩. 杜仲饲料添加剂的研究进展[J]. 饲料研究,2014 (19):29-31.
[6]	艾伦强, 李婷婷, 何银生,等. 杜仲的应用研究进展[J]. 亚太传统医药,2010(10):163-165.
[7]	董尚胜, 翁蔚, 查森俊,等. 杜仲茶风味化学的研究 Ⅲ.复火对杜仲红茶品质成分的影响[J]. 林业科学研究, 2001, 14(1):93-96.
[8]	冯晗,周宏灏,欧阳冬生.杜仲的化学成分及药理作用研究进展[J].中国临床药理学和治疗学,2015,20(6):713-720.
[9]	Danielle R, Paul P, Michael A. Applications of mass spectrometry to plant phenols [J]. Tr AC Trends in Analytical Chemistry, 1999,18(5):362-372.
[10]	刘迪, 尚华, 宋晓宇. 杜仲叶多酚体内和体外抗氧化活性[J]. 食品研究与开发, 2013(9):5-8.
[11]	赵子龙,薛培凤,倪佩东,等.天然药物中总黄酮的提取工艺研究进展[J].内蒙古医学院学报,2012,34(6):512-516.
[12]	孙兰萍, 马龙, 张斌,等. 杜仲黄酮类化合物的研究进展[J]. 食品工业科技, 2009(3):359-363.
[13]	李欣, 刘严, 朱文学,等. 杜仲的化学成分及药理作用研究进展[J]. 食品工业科技, 2012, 33(10):378-382.
[14]	王艳芳, 王新华, 朱宇同. 槲皮素药理作用研究进展[J]. 天然产物研究与开发, 2003, 15(2):171-173.
[15]	袁超. 异槲皮苷及其金属配合物的光谱特征、抗氧化特性及药代动力学研究[D]. 太原:山西大学, 2010.
[16]	张琳杰, 彭胜, 张昌伟,等. 响应面法优化杜仲叶中总多酚超声波辅助提取工艺研究[J]. 食品工业科技, 2014, 35(8):228-232.
[17]	Kwan C Y, Chen Changxun, DEYAMA Takeshi, et al. Endothelium-dependent vasorelaxant effects of the aqueous extracts of the Eucommia ulmoides Oliv. leaf and bark: implications on their antihy-pertensive action [J].Vascular Pharmacology, 2003,40 (5): 229-235.
[18]	Kim H Y, Moon B H, Lee H J, et al. Flavonol glycosides from the leaves of Eucommia ulmoides O. with glycation inhibitory activity[J]. Journal of Ethnopharmacology, 2004, 93(2-3):227-230.
[19]	Xican L, Xiaoting W, Ling H. Correlation between antioxidant activities and phenolic contents of radix Angelicae sinensis (Danggui).[J]. Molecules, 2009, 14(12):5349-5361.
[20]	Stankovic M S, Niciforovic N, Topuzovic M, et al.Total phenolic content,flavonoid concentrations and antioxidant activity, of the whole plant and parts extracts Teucrium montanum L. var. Montanum, F. Supinum (L.) Reichenb[J]. Biotechnology & Biotechnological Equipment, 2011, 25(25):2222-2227.
[21]	徐兰波,张峰,丁艳霞,等.HPLC法同时测定杜仲叶中6个黄酮类成分的含量[J].药物分析杂志,2014(8):1422-1425.
[22]	Zhang L, Ravipati A S, Koyyalamudi S R, et al. Antioxidant and anti-inflammatory activities of selected medicinal plants containing phenolic and flavonoid compounds[J]. Journal of agricultural and food chemistry, 2011, 59(23): 12361-12367.
[23]	欧阳玉祝,吴道宏,李勇.几种药用植物总多酚含量及其抗氧化活性比较[J].中国野生植物资源,2012,31(2):20-23.
[24]	仲凤维,王迎,万晓,等.6个银杏新品种叶片黄酮内酯含量的测定[J].山东林业科技,2013(5):20-22.
[25]	贾冬冬, 李淑芬, 杨鸿玲. RP-HPLC法测定桑叶中的芦丁和异槲皮苷含量[J]. 食品科学, 2008, 29(8):499-501.
[26]	李奇, 张月婵, 宋建平,等. 不同产地罗布麻叶黄酮类成分分析[J]. 中药材, 2009, 32(9):1359-1362.
[27]	周浓, 杨敏, 王胤,等. 商品川楝子的品质评价[J]. 中药材, 2012, 35(7):1065-1070.
[28]	马丽媛, 齐国辉, 李保国,等. 黄连木雌、雄株叶片几种保护酶活性及主要内含物含量的比较[J]. 河北农业大学学报, 2012, 35(2):48-52.
[29]	辜夕容.不同种源无患子的种子品质差异分析[J].西南大学学报:自然科学版,2009,31(6):51-54.
[30]	孙建,刘红艳,赵应忠,等.芝麻种质资源叶绿素含量的多样性分析[J]. 江西农业学报,2009,21(12):5-9.

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通讯作者: 陈斌, bchen63@163.com

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沈阳化工大学材料科学与工程学院沈阳 110142

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Comparison in Contents of Polyphenol and Flavonoid in Leaves of Eucommia ulmoides Germplasm

1. Non-timber Forest Research and Development Center of Chinese Academy of Forestry, the Eucommia Engineering Research Center of State Forestry Administration, Zhengzhou 450003, He'nan, China

Received Date: 2015-12-08

Abstract: [Objective] To compare the content difference of polyphenol and flavonoid in Eucommia ulmoides leaves, so as to evaluate and utilize E. ulmoides germplasm reasonably. [Method] HPLC and spectrophotometric methods were used to measure the contents of polyphenol, total flavonoid, isoquercitrin and quercetin of 105 E. ulmoides germplasm. [Result] The mean content of quercetin was 0.33 mg·g-1 with the highest variation coefficient of 42.42%, while the mean content of total flavonoid was 15.92 mg·g-1 with the lowest variation coefficient of 19.35%; the mean content of isoquercitrin, total polyphenol was 3.37 mg·g-1 and 42.74 mg·g-1, respectively, with the variation coefficient of 34.42% and 23.72%. No significant correlation was found in the contents of these four components between male and female plant leaves. There was highly significant difference in the four components among different regions (PE. ulmoides germplasm resources could be divided into four groups according to the content difference of the four components, in which the contents of flavonoid compounds and polyphenol in group Ⅲ including 13 materials were higher than that of the other groups. [Conclusion] The contents of polyphenol and flavonoid in leaves of E. ulmoides were high and abundant diversity and variation were found in different germplasms, which showed great potential for selection and improvement. Thus, it could provide basis materials for breeding Eucommia ulmoides germplasm.

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Reference (30)

[1]	杜红岩,胡文臻,俞锐.杜仲产业绿皮书[M].北京:社会科学文献出版社,2013.
[2]	杜红岩,赵戈.论我国杜仲产业化与培育技术的发展[J].林业科学研究,2000,13(5):554-561
[3]	黄海燕, 杜红岩, 乌云塔娜,等. 基于SSR分子标记的杜仲遗传多样性体系建立[J]. 林业科学研究, 2013, 26(6):795-799.
[4]	杜红岩. 杜仲活性成分与药理研究的新进展[J]. 经济林研究, 2004, 21(2):58-61.
[5]	王璐, 杜兰英, 杜红岩. 杜仲饲料添加剂的研究进展[J]. 饲料研究,2014 (19):29-31.
[6]	艾伦强, 李婷婷, 何银生,等. 杜仲的应用研究进展[J]. 亚太传统医药,2010(10):163-165.
[7]	董尚胜, 翁蔚, 查森俊,等. 杜仲茶风味化学的研究 Ⅲ.复火对杜仲红茶品质成分的影响[J]. 林业科学研究, 2001, 14(1):93-96.
[8]	冯晗,周宏灏,欧阳冬生.杜仲的化学成分及药理作用研究进展[J].中国临床药理学和治疗学,2015,20(6):713-720.
[9]	Danielle R, Paul P, Michael A. Applications of mass spectrometry to plant phenols [J]. Tr AC Trends in Analytical Chemistry, 1999,18(5):362-372.
[10]	刘迪, 尚华, 宋晓宇. 杜仲叶多酚体内和体外抗氧化活性[J]. 食品研究与开发, 2013(9):5-8.
[11]	赵子龙,薛培凤,倪佩东,等.天然药物中总黄酮的提取工艺研究进展[J].内蒙古医学院学报,2012,34(6):512-516.
[12]	孙兰萍, 马龙, 张斌,等. 杜仲黄酮类化合物的研究进展[J]. 食品工业科技, 2009(3):359-363.
[13]	李欣, 刘严, 朱文学,等. 杜仲的化学成分及药理作用研究进展[J]. 食品工业科技, 2012, 33(10):378-382.
[14]	王艳芳, 王新华, 朱宇同. 槲皮素药理作用研究进展[J]. 天然产物研究与开发, 2003, 15(2):171-173.
[15]	袁超. 异槲皮苷及其金属配合物的光谱特征、抗氧化特性及药代动力学研究[D]. 太原:山西大学, 2010.
[16]	张琳杰, 彭胜, 张昌伟,等. 响应面法优化杜仲叶中总多酚超声波辅助提取工艺研究[J]. 食品工业科技, 2014, 35(8):228-232.
[17]	Kwan C Y, Chen Changxun, DEYAMA Takeshi, et al. Endothelium-dependent vasorelaxant effects of the aqueous extracts of the Eucommia ulmoides Oliv. leaf and bark: implications on their antihy-pertensive action [J].Vascular Pharmacology, 2003,40 (5): 229-235.
[18]	Kim H Y, Moon B H, Lee H J, et al. Flavonol glycosides from the leaves of Eucommia ulmoides O. with glycation inhibitory activity[J]. Journal of Ethnopharmacology, 2004, 93(2-3):227-230.
[19]	Xican L, Xiaoting W, Ling H. Correlation between antioxidant activities and phenolic contents of radix Angelicae sinensis (Danggui).[J]. Molecules, 2009, 14(12):5349-5361.
[20]	Stankovic M S, Niciforovic N, Topuzovic M, et al.Total phenolic content,flavonoid concentrations and antioxidant activity, of the whole plant and parts extracts Teucrium montanum L. var. Montanum, F. Supinum (L.) Reichenb[J]. Biotechnology & Biotechnological Equipment, 2011, 25(25):2222-2227.
[21]	徐兰波,张峰,丁艳霞,等.HPLC法同时测定杜仲叶中6个黄酮类成分的含量[J].药物分析杂志,2014(8):1422-1425.
[22]	Zhang L, Ravipati A S, Koyyalamudi S R, et al. Antioxidant and anti-inflammatory activities of selected medicinal plants containing phenolic and flavonoid compounds[J]. Journal of agricultural and food chemistry, 2011, 59(23): 12361-12367.
[23]	欧阳玉祝,吴道宏,李勇.几种药用植物总多酚含量及其抗氧化活性比较[J].中国野生植物资源,2012,31(2):20-23.
[24]	仲凤维,王迎,万晓,等.6个银杏新品种叶片黄酮内酯含量的测定[J].山东林业科技,2013(5):20-22.
[25]	贾冬冬, 李淑芬, 杨鸿玲. RP-HPLC法测定桑叶中的芦丁和异槲皮苷含量[J]. 食品科学, 2008, 29(8):499-501.
[26]	李奇, 张月婵, 宋建平,等. 不同产地罗布麻叶黄酮类成分分析[J]. 中药材, 2009, 32(9):1359-1362.
[27]	周浓, 杨敏, 王胤,等. 商品川楝子的品质评价[J]. 中药材, 2012, 35(7):1065-1070.
[28]	马丽媛, 齐国辉, 李保国,等. 黄连木雌、雄株叶片几种保护酶活性及主要内含物含量的比较[J]. 河北农业大学学报, 2012, 35(2):48-52.
[29]	辜夕容.不同种源无患子的种子品质差异分析[J].西南大学学报:自然科学版,2009,31(6):51-54.
[30]	孙建,刘红艳,赵应忠,等.芝麻种质资源叶绿素含量的多样性分析[J]. 江西农业学报,2009,21(12):5-9.

Comparison in Contents of Polyphenol and Flavonoid in Leaves of Eucommia ulmoides Germplasm

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通讯作者: 陈斌, bchen63@163.com

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Comparison in Contents of Polyphenol and Flavonoid in Leaves of Eucommia ulmoides Germplasm

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