Effects of Zn Stress on Physiological Characteristics of Hylotelephium spectabile

WANG Ying; LIU Jing; YANG Guo-ting; GUAN Yang

Volume 29 Issue 4

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Article Navigation > Forest Research > 2016 > 29(4): 560-564

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Effects of Zn Stress on Physiological Characteristics of Hylotelephium spectabile

1.
Ecological Research Centre, College of Forestry, Northeast Forestry University, Harbin 150000, Heilongjiang, China
2.
Key Laboratory of Plant Biology, Harbin Normal University, Harbin 150025, Heilongjiang, China
3.
Forestry Bureau of Heilongjiang Province, Harbin 150000, Heilongjiang, China

Received Date: 2016-02-26

Abstract

[Objective] To study the tolerance of Hylotelephium spectabile to long-term ZnSO4 stress. [Method] Pot experiment was conducted to study the changes of leaves' physiological indexes of H. spectabile. The plants were treated with different concentrations ZnSO4·7H2O (0, 160 and 1 600 mg·kg-1) and the healthy leaves with the same size were collected as control material at the same position on the 0, 7th, 14th, 21th, 28th, and 35th days respectively. [Result] Under the stress of low concentration Zn, the electrical conductivity and the concentration of MDA of the leaf of H. spectabile didn't increased distinctively; the activity of protective enzyme such as SOD, POD and CAT increased; the concentration of proline, soluble sugar and soluble protein increased. Under the stress of high concentration Zn, the electrical conductivity and the concentration of MDA increased; the protective enzyme activity increased; the concentration of proline, soluble sugar and soluble protein changed slightly. [Conclusion] H. spectabile had a strong tolerance to Zn stress. Under low concentration Zn stress, H. spectabile showed a stronger ability to adjust, and under high concentration Zn stress, H. spectabile depended mainly on regulating protective enzyme system. In the process of continuous Zn stress, H. spectabile quickly made the corresponding physiological adaptation to Zn stress, and showed tolerance and a strong recovery ability.
- Hylotelephium spectabile,
- Zn stress,
- protective enzyme activity,
- osmoregulatory substance

References

[1]	PrabhuInbaraj Michael,Muthuchelian Krishnaswamy. The effect of zinc stress combined with high irradiance stress on membrane damage and antioxidative response in bean seedlings[J]. Environ Exp Bot, 2011, 74:171-177.
[2]	Mrozek J E, Funicelli N A. Effect of zinc and lead on germination of Spartina alterniflora Loisel seeds at various salinities[J].Environ Exp Bot, 1982,22: 23-32.
[3]	Lingua G, Franchin C, Todeschini V, et al. Arbuscular mycorrhizal fungi differentially affect the response to high zinc concentrations of two registered poplarclones[J].Environ Pollut,2008,153:137-147.
[4]	Ebbs S D, Kochian L V. Toxicity of zinc and copper to Brassica species: implications for phytoremediation[J]. J Environ Qual, 1997,26:776-781.
[5]	龚红梅, 李卫国. 锌对植物的毒害及机理研究进展[J].安徽农业科学, 2009, 37(29):14009-14015.
[6]	Stoyanova Z, Doncheva S. The effect of zinc supply and succinate treatmenton plant growth and mineral uptake in pea plant[J]. Braz J Plant Physiol, 2002, 14:111-116.
[7]	王学东, 周菊红, 华珞. 植物对重金属的抗性机理及其植物修复研究进展[J]. 南水北调与水利科技, 2006, 4(2):43-46.
[8]	李挺强,杨肖娥,龙新宪.东南景天提取污染土壤中锌的潜力研究[J].水土保持学报,2004,18(6):79-83.
[9]	Whiting S N, Leake J R, McGrath S P, et al. Hyperaccumulation of Zn by Thlaspi Caerulescens can ameliorate Zn toxicity in the rhizosphere of co-cropped Thlaspi Arvense[J].Environmental,2001,35:3237-3241.
[10]	科学出版社. 中国植物志[M]. 北京: 科学出版社, 2004.
[11]	中国科学院植物研究所. 中国高等植物图鉴(第二册)[M].北京: 科学出版社, 1985.
[12]	李丹, 罗方军, 雷国莲. 三七与景天三七化学成分的对比实验研究[J]. 陕西中医学院学报, 2007,30(1): 49-51.
[13]	焦轶男,朱宏.长药景天对重金属镉胁迫的生理及形态响应[J].西北植物学报,2014,34(6):1173-1178.
[14]	王艳,代保清,辛士刚,等.长药景天对Cr、Pb、Cd、Hg、Co复合污染的生理响应及吸收特征[J].农业环境科学学报,2008,27(3):1051-1056.
[15]	郝再彬,苍晶. 植物生理实验[M]. 哈尔滨:哈尔滨工业大学出版社, 2004:113-115,67-68.
[16]	中国科学院上海植物生理研究所,上海市植物生理学会. 现代植物生理学实验指南. 第二版[M]. 北京:科学出版社, 2004: 302-303,305-306,314-315,316,127-128,303,95-96.
[17]	Raman Bassi, Shanti S. Sharma.Proline acculation in Wheat seedlings Exposed to Zinc and Copper[J].Phytochemistry,1993,33(6): 1339-1342.
[18]	王广林,张金池,王丽,等.铜、锌胁迫对丁香蓼生理指标的影响[J].南京林业大学学报,2009.33(4):43-47.
[19]	原海燕,黄苏珍,郭智,等.锌对镉胁迫下马蔺生长、镉积累及生理抗性的影响[J].应用生态学报,2007,18(9):2111-2116.
[20]	何冰,叶海波,杨肖娥.铅胁迫下不同生态型东南景天叶片抗氧化酶活性及叶绿素含量比较[J].农业环境科学学报,2003,22(3):274-278.
[21]	张义贤,张丽萍.重金属对大麦幼苗膜脂过氧化及脯氨酸和可溶性糖含量的影响[J]. 农业环境科学学报, 2006, 25(4):857-860.
[22]	时丽冉,陈红艳,崔兴国.干旱胁迫对地被菊膜脂过氧化和抗氧化酶活性的影响[J]. 北方园艺, 2010(9):96-98.
[23]	吴灵琼.Cd²⁺和Cu²⁺对美人蕉的氧化胁迫及抗性机理研究[J]. 农业环境科学学报,2007,26(4):1365-369.
[24]	胡宗达,杨远祥,朱雪梅,等.Pb, Zn对超富集植物(小鳞苔草)抗氧化酶活性的影响[J].水土保持学报,2007,21(6):86-91.
[25]	Woxny A,Krzeslowka M.Plant cell response to Pb[J]. Acta Societatis Botanicorum Polonioe,1993,62:101-105.
[26]	Weckx J E,Clifsters H M M. Zn phytotoxicity induces oxidative stress in primary leaves of Phaseolus vulgaris[J].Plant Physiology and Biochemistry,1997,35(5):405-410.
[27]	Mazhoudi S,Chaoui A,Ghorbal M H,et al. Response of antioxidant enzymes to excess copper in tomato(Lycopersicon esculentum Mill)[J].Plant Science,1997,127:129-137.
[28]	Spickett C M, Smirnoff N, Ratdiffe R G. Metabolic responses of maize roots to hyperosmotic ahock[J]. Plant Physiol, 1992, 99: 856-863.
[29]	彭立新,李德全,束怀瑞.植物在渗透胁迫下的渗透调节作用[J]. 天津农业科学,2002, 8 (1): 40-43.
[30]	王娟,李健全.逆境条件下植物体内渗透调节物质的积累与活性氧代谢[J]. 植物学通报, 2001, 18 (4): 459-465.
[31]	韩豫, 曹莹, 王绍斌, 等.铅胁迫对花生生理生化特性的影响[J]. 花生学报,2007 , 36(1):24-27.
[32]	丁海东,朱为民,杨少军,等. 镉、锌胁迫对番茄幼苗生长及脯氨酸和谷胱甘肽含量的影响[J]. 江苏农业学报, 2005, 21 (3):191-196.
[33]	Costa G, Spitz E. Influence of cadmiumon soluble carbohydrates, free amino acids, protein content of in vitro cultured Lupinus albus[J].Plant Sci, 1997, 128: 131-140.
[34]	Prasad M N V. Cadmium toxicity and tolerance in vascular plants[J].Environ & Exp Bot,1995,35(4): 525-545.
[35]	Tang Z C. The accumulation of free proline and its roles in waterstressed sorghum seedings[J]. Acta Phytophy Sin, 1989, 15 (1): 105-110.
[36]	Schat H, Sharma S S, Vooijs R.Heavy metal-induced accumulation of free proline in a metal-tolerant and a nontolerant ecotype of Silene vulgaris[J]. Physiol Planta, 1997, 101: 477-482.
[37]	冀宪领, 盖英萍, 刘训理, 等. 干旱胁迫对桑树生理生化特性的影响[J]. 蚕业科学, 2004, 30(2):117-122..
[38]	吴桂容,严重玲.镉对桐花树幼苗生长及渗透调节的影响[J]. 生态环境, 2006, 15 (5): 1003-1008.
[39]	李兆君,马国瑞,徐建民,等.植物适应重金属 C d 胁迫的生理及分子生物学机理[J]. 土壤通报, 2004, 35 (2): 234-238.
[40]	孙赛初,王焕校,李启任.水生维管束植物受锦污染后的生理变化及受害机制初探[J].植物生理学报,1985,11(2):113-121.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Effects of Zn Stress on Physiological Characteristics of Hylotelephium spectabile

1. Ecological Research Centre, College of Forestry, Northeast Forestry University, Harbin 150000, Heilongjiang, China

2. Key Laboratory of Plant Biology, Harbin Normal University, Harbin 150025, Heilongjiang, China

3. Forestry Bureau of Heilongjiang Province, Harbin 150000, Heilongjiang, China

Received Date: 2016-02-26

Abstract: [Objective] To study the tolerance of Hylotelephium spectabile to long-term ZnSO4 stress. [Method] Pot experiment was conducted to study the changes of leaves' physiological indexes of H. spectabile. The plants were treated with different concentrations ZnSO4·7H2O (0, 160 and 1 600 mg·kg-1) and the healthy leaves with the same size were collected as control material at the same position on the 0, 7th, 14th, 21th, 28th, and 35th days respectively. [Result] Under the stress of low concentration Zn, the electrical conductivity and the concentration of MDA of the leaf of H. spectabile didn't increased distinctively; the activity of protective enzyme such as SOD, POD and CAT increased; the concentration of proline, soluble sugar and soluble protein increased. Under the stress of high concentration Zn, the electrical conductivity and the concentration of MDA increased; the protective enzyme activity increased; the concentration of proline, soluble sugar and soluble protein changed slightly. [Conclusion] H. spectabile had a strong tolerance to Zn stress. Under low concentration Zn stress, H. spectabile showed a stronger ability to adjust, and under high concentration Zn stress, H. spectabile depended mainly on regulating protective enzyme system. In the process of continuous Zn stress, H. spectabile quickly made the corresponding physiological adaptation to Zn stress, and showed tolerance and a strong recovery ability.

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Reference (40)

[1]	PrabhuInbaraj Michael,Muthuchelian Krishnaswamy. The effect of zinc stress combined with high irradiance stress on membrane damage and antioxidative response in bean seedlings[J]. Environ Exp Bot, 2011, 74:171-177.
[2]	Mrozek J E, Funicelli N A. Effect of zinc and lead on germination of Spartina alterniflora Loisel seeds at various salinities[J].Environ Exp Bot, 1982,22: 23-32.
[3]	Lingua G, Franchin C, Todeschini V, et al. Arbuscular mycorrhizal fungi differentially affect the response to high zinc concentrations of two registered poplarclones[J].Environ Pollut,2008,153:137-147.
[4]	Ebbs S D, Kochian L V. Toxicity of zinc and copper to Brassica species: implications for phytoremediation[J]. J Environ Qual, 1997,26:776-781.
[5]	龚红梅, 李卫国. 锌对植物的毒害及机理研究进展[J].安徽农业科学, 2009, 37(29):14009-14015.
[6]	Stoyanova Z, Doncheva S. The effect of zinc supply and succinate treatmenton plant growth and mineral uptake in pea plant[J]. Braz J Plant Physiol, 2002, 14:111-116.
[7]	王学东, 周菊红, 华珞. 植物对重金属的抗性机理及其植物修复研究进展[J]. 南水北调与水利科技, 2006, 4(2):43-46.
[8]	李挺强,杨肖娥,龙新宪.东南景天提取污染土壤中锌的潜力研究[J].水土保持学报,2004,18(6):79-83.
[9]	Whiting S N, Leake J R, McGrath S P, et al. Hyperaccumulation of Zn by Thlaspi Caerulescens can ameliorate Zn toxicity in the rhizosphere of co-cropped Thlaspi Arvense[J].Environmental,2001,35:3237-3241.
[10]	科学出版社. 中国植物志[M]. 北京: 科学出版社, 2004.
[11]	中国科学院植物研究所. 中国高等植物图鉴(第二册)[M].北京: 科学出版社, 1985.
[12]	李丹, 罗方军, 雷国莲. 三七与景天三七化学成分的对比实验研究[J]. 陕西中医学院学报, 2007,30(1): 49-51.
[13]	焦轶男,朱宏.长药景天对重金属镉胁迫的生理及形态响应[J].西北植物学报,2014,34(6):1173-1178.
[14]	王艳,代保清,辛士刚,等.长药景天对Cr、Pb、Cd、Hg、Co复合污染的生理响应及吸收特征[J].农业环境科学学报,2008,27(3):1051-1056.
[15]	郝再彬,苍晶. 植物生理实验[M]. 哈尔滨:哈尔滨工业大学出版社, 2004:113-115,67-68.
[16]	中国科学院上海植物生理研究所,上海市植物生理学会. 现代植物生理学实验指南. 第二版[M]. 北京:科学出版社, 2004: 302-303,305-306,314-315,316,127-128,303,95-96.
[17]	Raman Bassi, Shanti S. Sharma.Proline acculation in Wheat seedlings Exposed to Zinc and Copper[J].Phytochemistry,1993,33(6): 1339-1342.
[18]	王广林,张金池,王丽,等.铜、锌胁迫对丁香蓼生理指标的影响[J].南京林业大学学报,2009.33(4):43-47.
[19]	原海燕,黄苏珍,郭智,等.锌对镉胁迫下马蔺生长、镉积累及生理抗性的影响[J].应用生态学报,2007,18(9):2111-2116.
[20]	何冰,叶海波,杨肖娥.铅胁迫下不同生态型东南景天叶片抗氧化酶活性及叶绿素含量比较[J].农业环境科学学报,2003,22(3):274-278.
[21]	张义贤,张丽萍.重金属对大麦幼苗膜脂过氧化及脯氨酸和可溶性糖含量的影响[J]. 农业环境科学学报, 2006, 25(4):857-860.
[22]	时丽冉,陈红艳,崔兴国.干旱胁迫对地被菊膜脂过氧化和抗氧化酶活性的影响[J]. 北方园艺, 2010(9):96-98.
[23]	吴灵琼.Cd²⁺和Cu²⁺对美人蕉的氧化胁迫及抗性机理研究[J]. 农业环境科学学报,2007,26(4):1365-369.
[24]	胡宗达,杨远祥,朱雪梅,等.Pb, Zn对超富集植物(小鳞苔草)抗氧化酶活性的影响[J].水土保持学报,2007,21(6):86-91.
[25]	Woxny A,Krzeslowka M.Plant cell response to Pb[J]. Acta Societatis Botanicorum Polonioe,1993,62:101-105.
[26]	Weckx J E,Clifsters H M M. Zn phytotoxicity induces oxidative stress in primary leaves of Phaseolus vulgaris[J].Plant Physiology and Biochemistry,1997,35(5):405-410.
[27]	Mazhoudi S,Chaoui A,Ghorbal M H,et al. Response of antioxidant enzymes to excess copper in tomato(Lycopersicon esculentum Mill)[J].Plant Science,1997,127:129-137.
[28]	Spickett C M, Smirnoff N, Ratdiffe R G. Metabolic responses of maize roots to hyperosmotic ahock[J]. Plant Physiol, 1992, 99: 856-863.
[29]	彭立新,李德全,束怀瑞.植物在渗透胁迫下的渗透调节作用[J]. 天津农业科学,2002, 8 (1): 40-43.
[30]	王娟,李健全.逆境条件下植物体内渗透调节物质的积累与活性氧代谢[J]. 植物学通报, 2001, 18 (4): 459-465.
[31]	韩豫, 曹莹, 王绍斌, 等.铅胁迫对花生生理生化特性的影响[J]. 花生学报,2007 , 36(1):24-27.
[32]	丁海东,朱为民,杨少军,等. 镉、锌胁迫对番茄幼苗生长及脯氨酸和谷胱甘肽含量的影响[J]. 江苏农业学报, 2005, 21 (3):191-196.
[33]	Costa G, Spitz E. Influence of cadmiumon soluble carbohydrates, free amino acids, protein content of in vitro cultured Lupinus albus[J].Plant Sci, 1997, 128: 131-140.
[34]	Prasad M N V. Cadmium toxicity and tolerance in vascular plants[J].Environ & Exp Bot,1995,35(4): 525-545.
[35]	Tang Z C. The accumulation of free proline and its roles in waterstressed sorghum seedings[J]. Acta Phytophy Sin, 1989, 15 (1): 105-110.
[36]	Schat H, Sharma S S, Vooijs R.Heavy metal-induced accumulation of free proline in a metal-tolerant and a nontolerant ecotype of Silene vulgaris[J]. Physiol Planta, 1997, 101: 477-482.
[37]	冀宪领, 盖英萍, 刘训理, 等. 干旱胁迫对桑树生理生化特性的影响[J]. 蚕业科学, 2004, 30(2):117-122..
[38]	吴桂容,严重玲.镉对桐花树幼苗生长及渗透调节的影响[J]. 生态环境, 2006, 15 (5): 1003-1008.
[39]	李兆君,马国瑞,徐建民,等.植物适应重金属 C d 胁迫的生理及分子生物学机理[J]. 土壤通报, 2004, 35 (2): 234-238.
[40]	孙赛初,王焕校,李启任.水生维管束植物受锦污染后的生理变化及受害机制初探[J].植物生理学报,1985,11(2):113-121.

Effects of Zn Stress on Physiological Characteristics of Hylotelephium spectabile

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通讯作者: 陈斌, bchen63@163.com

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Effects of Zn Stress on Physiological Characteristics of Hylotelephium spectabile

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