Screening and Identification of Antagonistic Bacteria Against Three Pathogenic Fungi of Orchid

WANG Shi-yan; XU Lu; WEI Li; CAO Xiao-lu; LIU Lei; LI Lu-bin

Volume 29 Issue 5

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Screening and Identification of Antagonistic Bacteria Against Three Pathogenic Fungi of Orchid

1.
Research Institute of Forestry, Chinese Academy of Forestry, State Key Laboratory of Tree Genetics and Breeding, Beijing 100091, China
2.
Environmental Manitering Center of Handan, Handan Hebei, 056002, China

Received Date: 2015-12-21

Abstract

[Objective] The purpose of this study is to obtain the bacterial strains with high antagonism against Colletotrichum gloeosporioides, Fusarium oxysporum and F. solani, the fungal pathogens of orchid. [Method] In this study,the method of dual culture on plate was used to screen the bacterial strains. [Result] It was found that there were 5 strains which could inhibit all the three pathogens and the strain GT312 had the highest antagonistic activity. The results of phylogenetic analysis of 16S rRNA gene sequence revealed that GT312 had 99.93% similarity with Bacillus amyloliquefaciens type strain FZB42T. [Conclusion] Based on the morphologic and physiological characteristics, the strain GT312 was identified as B. amyloliquefaciens. It could be used as a potential bio-control agent of orchid diseases.
- Colletotrichum gloeosporioides,
- Fusarium oxysporum,
- Fusarium solani,
- antagonism,
- screening,
- identify,
- Bacillus amyloliquefaciens

References

[1]	易绮斐,刘东明,陈红锋,等. 兰花主要病害及其防治[J]. 植物保护,2004,30(1):71-73.
[2]	姜英华,胡白石,刘凤权. 植物土传病原菌拮抗细菌的筛选与鉴定[J]. 中国生物防治,2005,21(4):260-264.
[3]	李潞滨,李术娜,李佳,等. 大花惠兰根腐病拮抗细菌ZL7-5菌株的筛选与鉴定[J]. 园艺学报,2008,35(11):1647-1652.
[4]	毕博,孟庆龙,包京姗. 玉竹褐斑病拮抗细菌的分离筛选和鉴定[J]. 生物技术通报,2015,31(9):152-157.
[5]	El Arbi A,Rochex A,Chataigné G,et al. The Tunisian oasis ecosystem is a source of antagonistic Bacillus spp. producing diverse antifungal lipopeptides[J]. Research in microbiology,2016,167(1):46-57.
[6]	葛慈斌,刘波,蓝江林,等. 生防菌JK-2对尖孢镰孢菌抑制特性的研究[J]. 福建农业学报,2009,24(1):29-34.
[7]	张晶. 库姆塔格沙漠植物相关细菌多样性研究[D]. 中国林业科学研究院:中国林业科学研究院,2014.
[8]	王华磊. 结皮微生物资源菌株筛选及其代谢产物研究[D]. 河北联合大学:河北联合大学,2014.
[9]	秦晓丹. 金佛山兰内生及根圈微生物多样性[D]. 中国林业科学研究院:中国林业科学研究院,2014.
[10]	沈萍,陈向东. 微生物学实验(第4版)[M]. 北京:高等教育出版社,2010.
[11]	东秀珠,蔡妙英. 《常见细菌系统鉴定手册》第一版[M]. 北京:科学出版社,2001.
[12]	布坎南,吉本斯. 《伯杰细菌鉴定手册》(第八版)[M]. 北京:科学出版社,1984.
[13]	许璐. 库姆塔格沙漠土壤微生物多样性研究[D]. 中国林业科学研究院:中国林业科学研究院,2014.
[14]	卢彩鸽,张殿朋,刘伟成,等. 一株甘蓝枯萎病拮抗细菌的筛选、鉴定及其抑菌活性测定[J]. 华北农学报,2014,29(1):195-202.
[15]	Priest F G,Goodfellow M,Shute L A,et al. Bacillus amyloliquefaciens sp.nov., nom.rev[J]. International Journal of Systematic and Evolutionary Microbiology,1987,37(1):69-71.
[16]	庞丽杰,王文革. Streptomyces sp.(S-msu2)菌株对几种树木病原拮抗效应的研究[J]. 防护林科技,2015,(12):33-35.
[17]	李潞滨,庄彩云,李术娜,等. 兰花炭疽病拮抗细菌8-59菌株的分离与鉴定[J]. 河北农业大学学报,2008,31(3):65-68.
[18]	曹晓璐,刘慧娟,郭晓军,等. 兰花炭疽病拮抗细菌5A5-3菌株的初步筛选和鉴定[J]. 林业科学研究,2013,26(5):598-602.
[19]	吕纪涛,魏莉,曹晓璐,等. 胶孢炭疽菌拮抗菌株的筛选、鉴定及抑菌物质分析[J]. 河南农业科学,2015,44(7):89-93.
[20]	连彩,郭晓军,朱宝成,等. 兰花枯萎病拮抗细菌的筛选与鉴定[J]. 华北农学报,2012,27(2):222-225.
[21]	王倩,李术娜,朱宝成,等. 兰花炭疽病拮抗细菌Bacillus megaterium 1-12菌株的产芽孢条件优化[J]. 河北大学学报(自然科学版),2008,(5):523-528.
[22]	Sajitha K L,Dev S A,Quantification of antifungal lipopeptide gene expression levels in Bacillus subtilis B1 during antagonism against sapstain fungus on rubberwood[J]. Biological Control,2016,96:78-85.
[23]	谢丽华,高虹,陈明丽,等. 枯草芽孢杆菌SB-24对尖孢镰孢菌拮抗机理[J]. 土壤与作物,2015,4(2):91-95.
[24]	黄曦,许兰兰,黄荣韶,等. 枯草芽孢杆菌在抑制植物病原菌中的研究进展[J]. 生物技术通报,2010,(1):24-28.
[25]	Di Francesco A,Martini C,Mari M. Biological control of postharvest diseases by microbial antagonists:how many mechanisms of action[J]. European Journal of Plant Pathology,2016:1-7.
[26]	Sen S, Rai M,Acharya R,et al. Biological control of pathogens causing the Cymbidium pseudobulb rot complex using fluorescent Pseudomonas strain BRL-1[J]. Journal of Plant Pathology,2009:751-755.
[27]	Idris E S E,Iglesias D J,Talon M,et al. Tryptophan-dependent production of indole-3-acetic acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42[J]. Molecular Plant-Microbe Interactions,2007,20(6):619-626.
[28]	Chen X H,Koumoutsi A,Scholz R,et al. Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42[J]. Nature biotechnology,2007,25(9):1007-1014.
[29]	Chen X,Koumoutsi A,Scholz R,et al. More than anticipated-production of antibiotics and other secondary metabolites by Bacillus amyloliquefaciens FZB42[J]. Journal of molecular microbiology and biotechnology,2008,16(1-2):14-24.
[30]	王茹华,张启发,周宝利,等. 浅析植物根分泌物与根际微生物的相互作用关系[J]. 土壤通报,2007,38(1):167-172.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Screening and Identification of Antagonistic Bacteria Against Three Pathogenic Fungi of Orchid

1. Research Institute of Forestry, Chinese Academy of Forestry, State Key Laboratory of Tree Genetics and Breeding, Beijing 100091, China

2. Environmental Manitering Center of Handan, Handan Hebei, 056002, China

Received Date: 2015-12-21

Abstract: [Objective] The purpose of this study is to obtain the bacterial strains with high antagonism against Colletotrichum gloeosporioides, Fusarium oxysporum and F. solani, the fungal pathogens of orchid. [Method] In this study,the method of dual culture on plate was used to screen the bacterial strains. [Result] It was found that there were 5 strains which could inhibit all the three pathogens and the strain GT312 had the highest antagonistic activity. The results of phylogenetic analysis of 16S rRNA gene sequence revealed that GT312 had 99.93% similarity with Bacillus amyloliquefaciens type strain FZB42T. [Conclusion] Based on the morphologic and physiological characteristics, the strain GT312 was identified as B. amyloliquefaciens. It could be used as a potential bio-control agent of orchid diseases.

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Reference (30)

[1]	易绮斐,刘东明,陈红锋,等. 兰花主要病害及其防治[J]. 植物保护,2004,30(1):71-73.
[2]	姜英华,胡白石,刘凤权. 植物土传病原菌拮抗细菌的筛选与鉴定[J]. 中国生物防治,2005,21(4):260-264.
[3]	李潞滨,李术娜,李佳,等. 大花惠兰根腐病拮抗细菌ZL7-5菌株的筛选与鉴定[J]. 园艺学报,2008,35(11):1647-1652.
[4]	毕博,孟庆龙,包京姗. 玉竹褐斑病拮抗细菌的分离筛选和鉴定[J]. 生物技术通报,2015,31(9):152-157.
[5]	El Arbi A,Rochex A,Chataigné G,et al. The Tunisian oasis ecosystem is a source of antagonistic Bacillus spp. producing diverse antifungal lipopeptides[J]. Research in microbiology,2016,167(1):46-57.
[6]	葛慈斌,刘波,蓝江林,等. 生防菌JK-2对尖孢镰孢菌抑制特性的研究[J]. 福建农业学报,2009,24(1):29-34.
[7]	张晶. 库姆塔格沙漠植物相关细菌多样性研究[D]. 中国林业科学研究院:中国林业科学研究院,2014.
[8]	王华磊. 结皮微生物资源菌株筛选及其代谢产物研究[D]. 河北联合大学:河北联合大学,2014.
[9]	秦晓丹. 金佛山兰内生及根圈微生物多样性[D]. 中国林业科学研究院:中国林业科学研究院,2014.
[10]	沈萍,陈向东. 微生物学实验(第4版)[M]. 北京:高等教育出版社,2010.
[11]	东秀珠,蔡妙英. 《常见细菌系统鉴定手册》第一版[M]. 北京:科学出版社,2001.
[12]	布坎南,吉本斯. 《伯杰细菌鉴定手册》(第八版)[M]. 北京:科学出版社,1984.
[13]	许璐. 库姆塔格沙漠土壤微生物多样性研究[D]. 中国林业科学研究院:中国林业科学研究院,2014.
[14]	卢彩鸽,张殿朋,刘伟成,等. 一株甘蓝枯萎病拮抗细菌的筛选、鉴定及其抑菌活性测定[J]. 华北农学报,2014,29(1):195-202.
[15]	Priest F G,Goodfellow M,Shute L A,et al. Bacillus amyloliquefaciens sp.nov., nom.rev[J]. International Journal of Systematic and Evolutionary Microbiology,1987,37(1):69-71.
[16]	庞丽杰,王文革. Streptomyces sp.(S-msu2)菌株对几种树木病原拮抗效应的研究[J]. 防护林科技,2015,(12):33-35.
[17]	李潞滨,庄彩云,李术娜,等. 兰花炭疽病拮抗细菌8-59菌株的分离与鉴定[J]. 河北农业大学学报,2008,31(3):65-68.
[18]	曹晓璐,刘慧娟,郭晓军,等. 兰花炭疽病拮抗细菌5A5-3菌株的初步筛选和鉴定[J]. 林业科学研究,2013,26(5):598-602.
[19]	吕纪涛,魏莉,曹晓璐,等. 胶孢炭疽菌拮抗菌株的筛选、鉴定及抑菌物质分析[J]. 河南农业科学,2015,44(7):89-93.
[20]	连彩,郭晓军,朱宝成,等. 兰花枯萎病拮抗细菌的筛选与鉴定[J]. 华北农学报,2012,27(2):222-225.
[21]	王倩,李术娜,朱宝成,等. 兰花炭疽病拮抗细菌Bacillus megaterium 1-12菌株的产芽孢条件优化[J]. 河北大学学报(自然科学版),2008,(5):523-528.
[22]	Sajitha K L,Dev S A,Quantification of antifungal lipopeptide gene expression levels in Bacillus subtilis B1 during antagonism against sapstain fungus on rubberwood[J]. Biological Control,2016,96:78-85.
[23]	谢丽华,高虹,陈明丽,等. 枯草芽孢杆菌SB-24对尖孢镰孢菌拮抗机理[J]. 土壤与作物,2015,4(2):91-95.
[24]	黄曦,许兰兰,黄荣韶,等. 枯草芽孢杆菌在抑制植物病原菌中的研究进展[J]. 生物技术通报,2010,(1):24-28.
[25]	Di Francesco A,Martini C,Mari M. Biological control of postharvest diseases by microbial antagonists:how many mechanisms of action[J]. European Journal of Plant Pathology,2016:1-7.
[26]	Sen S, Rai M,Acharya R,et al. Biological control of pathogens causing the Cymbidium pseudobulb rot complex using fluorescent Pseudomonas strain BRL-1[J]. Journal of Plant Pathology,2009:751-755.
[27]	Idris E S E,Iglesias D J,Talon M,et al. Tryptophan-dependent production of indole-3-acetic acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42[J]. Molecular Plant-Microbe Interactions,2007,20(6):619-626.
[28]	Chen X H,Koumoutsi A,Scholz R,et al. Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42[J]. Nature biotechnology,2007,25(9):1007-1014.
[29]	Chen X,Koumoutsi A,Scholz R,et al. More than anticipated-production of antibiotics and other secondary metabolites by Bacillus amyloliquefaciens FZB42[J]. Journal of molecular microbiology and biotechnology,2008,16(1-2):14-24.
[30]	王茹华,张启发,周宝利,等. 浅析植物根分泌物与根际微生物的相互作用关系[J]. 土壤通报,2007,38(1):167-172.

Screening and Identification of Antagonistic Bacteria Against Three Pathogenic Fungi of Orchid

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通讯作者: 陈斌, bchen63@163.com

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Screening and Identification of Antagonistic Bacteria Against Three Pathogenic Fungi of Orchid

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