Sequence Analysis of Heat Shock Protein of Ericerus pela

SUN Tao; WANG Xue-qing; ZHAO Zun-ling; YU Shu-hui; CHEN Xiao-ming; LIU Wei-wei; QI Qian; YANG Pu

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Sequence Analysis of Heat Shock Protein of Ericerus pela

1.
Research Institute of Resources Insects, Chinese Academy of Forestry, Key Laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2016-03-06

Abstract

[Objective] The study aims at investigating the heat shock protein (hsp) genes and analyze the sequence characterization of Ericerus pela during the peak period of wax-secreting. [Method] The sequences of hsp10, hsp20, hsp40, hsp60, hsp70, and hsp90 were obtained from the transcriptome database of E. pela. The sequence alignment and phylogenetic analysis were performed. And the mRNA expression patterns of hsp40, hsp60, and hsp90 under different temperature treatments were carried out by using the quantitative real time PCR (qRT-PCR). [Result] A total of 32 HSPs, which were highly similar with the HSP reported in other species, were identified. Most of them had the conserved regions and characteristic sequences, and they were clustered together with the sequences of the same order insect in the phylogenetic trees. The qRT-PCR results showed that, hsp40, hsp60, and hsp90 can be induced by temperature-stress. [Conclusion] In this study, 32 unigenes of HSP of E. pela were identified, including 3 hsp10 unigenes, 1 hsp20 unigenes, 11 hsp40 unigenes, 1 hsp60 unigenes, 13 hsp70 unigenes, and 3 hsp90 unigenes.
- Ericerus pela,
- heat shock protein,
- sequence analysis,
- temperature-stress,
- quantitative real time PCR

References

[1]	陈小平.白蜡虫的研究进展[J].四川林业科技, 2007, 28(1):50-52.
[2]	张长海.白蜡虫在我国的地理分布[J].林业科学研究, 1991, 4(2):192-196.
[3]	陈晓鸣,王自力,陈勇,等.影响白蜡虫泌蜡主要气候因子及白蜡虫生态适应性分析[J].昆虫学报, 2007, 50(2):45-52.
[4]	陈晓鸣,王自力,陈勇,等.白蜡虫同地产虫产蜡生产模式构建与分析[J].林业科学, 2010, 46(12):106-113.
[5]	Rinehart JP, Li A, Yocum GD, et al. Up-regulation of heat shock proteins is essential for cold survival during insect diapause[J].Proceedings of the National Academy of Sciences.2007,104(27):11130-11137.
[6]	King AM and MacRae TH. Insect heat shock proteins during stress and diapause[J].Annual Review of Entomology,2015,60(1):59-75.
[7]	Štětina T, Koštál V and Korbelová J. The Role of Inducible Hsp70,and Other Heat Shock Proteins, in Adaptive Complex of Cold Tolerance of the Fruit Fly(Drosophila melanogaster)[J].PLoS One,2015,10(6):1-22.
[8]	Zhu JY, Zhao N and Yang B. Global transcriptome profiling of the pine shoot beetle, Tomicus yunnanensis(Coleoptera:Scolytinae)[J].PLoS One.2012,7(2):1-12.
[9]	Wang H, Li K, Zhu JY, et al. Cloning and expression pattern of heat shock protein genes from the endoparasitoid wasp, Pteromalus puparum in response to environmental stresses[J].Archives of Insect Biochemistry and Physiology. 2012,79(4-5):247-63.
[10]	Tungjitwitayakul J, Tatun N, Vajarasathira B, et al. Expression of heat shock protein genes in different developmental stages and after temperature stress in the Maize weevil (Coleoptera:Curculionidae)[J].J Econ Entomol.2015,108(3):1313-1323.
[11]	刘魏魏,杨璞,陈晓鸣.白蜡虫热激蛋白基因在低温胁迫下的表达分析[J].林业科学研究,2013,26(6):681-685.
[12]	Liu W W, Yang P, Chen X M, et al. Cloning and expression analysis of four heat shock protein genes in Ericerus pela(Homoptera:Coccidae)[J].Journal of Insect Science.2014,14(142):1-9
[13]	Wang H S, Wang X H, Zhou C S, et al. cDNA cloning of heat shock proteins and their expression in the two phases of the migratory locust[J]. Insect Molecular Biology.2007,16(2):207-219.
[14]	Yang P, Zhu J Y, Gong Z J, et al. Transcriptome analysis of the Chinese white wax scale Ericerus pela with focus on genes involved in wax biosynthesis[J].PLoS One.2012,7(4):e35719.
[15]	Lu M X, Hua J, Cui Y D, et al. Five small heat shock protein genes from Chilo suppressalis: characteristics of gene, genomic organization, structural analysis, and transcription profiles[J].Cell Stress Chaperones.2014,19(1):91-104.
[16]	Qiu X B, Shao Y M, Miao S, et al. The diversity of the Dna J/Hsp40 family, the crucial partners for Hsp70 chaperones[J].Cellular and Molecular Life Sciences.2006,63(22):2560-2570.
[17]	Colinet H, Lee S F, Hoffmann A. Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster[J].Febs Journal.2010,277(1):174-185.
[18]	Itoh H, Komatsuda A, Ohtani H, et al. Mammalian HSP60 is quickly sorted into the mitochondria under conditions of dehydration[J].European Journal of Biochemistry.2002,269(23):5931-5938.
[19]	Cui Y D, Du Y Z, Lu M X, et al. Cloning of the heat shock protein 60 gene from the stem borer, Chilo suppressalis, and analysis of expression characteristics under heat stress[J].Journal of Insect Science.2010, 10(100):100.
[20]	Wang H, Lei Z, Li X, et al. Rapid cold hardening and expression of heat shock protein genes in the B-bio type Bemisia tabaci[J].Environmental Entomology.2011,40(1):132-139.
[21]	Rinehart J P, Denlinger D L. Heat-shock protein 90 is down-regulated during pupal diapause in the flesh fly, Sarcophaga crassipalpis, but remains responsive to thermal stress[J].Insect Molecular Biology.2000,9(6):641-645.
[22]	Shue G, Kohtz D S. Structural and functional aspects of basic helix-loop-helix protein folding byheat-shock protein 90[J].Journal of Biological Chemistry.1994,269(4):2707-2711.
[23]	Li Q R, Xiao Y, Wu F Q, et al. Analysis of midgut gene expression profiles from different silkworm varieties after exposure to high temperature[J].Gene.2014,549(1):85-96.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Sequence Analysis of Heat Shock Protein of Ericerus pela

1. Research Institute of Resources Insects, Chinese Academy of Forestry, Key Laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming 650224, Yunnan, China

Received Date: 2016-03-06

Abstract: [Objective] The study aims at investigating the heat shock protein (hsp) genes and analyze the sequence characterization of Ericerus pela during the peak period of wax-secreting. [Method] The sequences of hsp10, hsp20, hsp40, hsp60, hsp70, and hsp90 were obtained from the transcriptome database of E. pela. The sequence alignment and phylogenetic analysis were performed. And the mRNA expression patterns of hsp40, hsp60, and hsp90 under different temperature treatments were carried out by using the quantitative real time PCR (qRT-PCR). [Result] A total of 32 HSPs, which were highly similar with the HSP reported in other species, were identified. Most of them had the conserved regions and characteristic sequences, and they were clustered together with the sequences of the same order insect in the phylogenetic trees. The qRT-PCR results showed that, hsp40, hsp60, and hsp90 can be induced by temperature-stress. [Conclusion] In this study, 32 unigenes of HSP of E. pela were identified, including 3 hsp10 unigenes, 1 hsp20 unigenes, 11 hsp40 unigenes, 1 hsp60 unigenes, 13 hsp70 unigenes, and 3 hsp90 unigenes.

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Reference (23)

[1]	陈小平.白蜡虫的研究进展[J].四川林业科技, 2007, 28(1):50-52.
[2]	张长海.白蜡虫在我国的地理分布[J].林业科学研究, 1991, 4(2):192-196.
[3]	陈晓鸣,王自力,陈勇,等.影响白蜡虫泌蜡主要气候因子及白蜡虫生态适应性分析[J].昆虫学报, 2007, 50(2):45-52.
[4]	陈晓鸣,王自力,陈勇,等.白蜡虫同地产虫产蜡生产模式构建与分析[J].林业科学, 2010, 46(12):106-113.
[5]	Rinehart JP, Li A, Yocum GD, et al. Up-regulation of heat shock proteins is essential for cold survival during insect diapause[J].Proceedings of the National Academy of Sciences.2007,104(27):11130-11137.
[6]	King AM and MacRae TH. Insect heat shock proteins during stress and diapause[J].Annual Review of Entomology,2015,60(1):59-75.
[7]	Štětina T, Koštál V and Korbelová J. The Role of Inducible Hsp70,and Other Heat Shock Proteins, in Adaptive Complex of Cold Tolerance of the Fruit Fly(Drosophila melanogaster)[J].PLoS One,2015,10(6):1-22.
[8]	Zhu JY, Zhao N and Yang B. Global transcriptome profiling of the pine shoot beetle, Tomicus yunnanensis(Coleoptera:Scolytinae)[J].PLoS One.2012,7(2):1-12.
[9]	Wang H, Li K, Zhu JY, et al. Cloning and expression pattern of heat shock protein genes from the endoparasitoid wasp, Pteromalus puparum in response to environmental stresses[J].Archives of Insect Biochemistry and Physiology. 2012,79(4-5):247-63.
[10]	Tungjitwitayakul J, Tatun N, Vajarasathira B, et al. Expression of heat shock protein genes in different developmental stages and after temperature stress in the Maize weevil (Coleoptera:Curculionidae)[J].J Econ Entomol.2015,108(3):1313-1323.
[11]	刘魏魏,杨璞,陈晓鸣.白蜡虫热激蛋白基因在低温胁迫下的表达分析[J].林业科学研究,2013,26(6):681-685.
[12]	Liu W W, Yang P, Chen X M, et al. Cloning and expression analysis of four heat shock protein genes in Ericerus pela(Homoptera:Coccidae)[J].Journal of Insect Science.2014,14(142):1-9
[13]	Wang H S, Wang X H, Zhou C S, et al. cDNA cloning of heat shock proteins and their expression in the two phases of the migratory locust[J]. Insect Molecular Biology.2007,16(2):207-219.
[14]	Yang P, Zhu J Y, Gong Z J, et al. Transcriptome analysis of the Chinese white wax scale Ericerus pela with focus on genes involved in wax biosynthesis[J].PLoS One.2012,7(4):e35719.
[15]	Lu M X, Hua J, Cui Y D, et al. Five small heat shock protein genes from Chilo suppressalis: characteristics of gene, genomic organization, structural analysis, and transcription profiles[J].Cell Stress Chaperones.2014,19(1):91-104.
[16]	Qiu X B, Shao Y M, Miao S, et al. The diversity of the Dna J/Hsp40 family, the crucial partners for Hsp70 chaperones[J].Cellular and Molecular Life Sciences.2006,63(22):2560-2570.
[17]	Colinet H, Lee S F, Hoffmann A. Temporal expression of heat shock genes during cold stress and recovery from chill coma in adult Drosophila melanogaster[J].Febs Journal.2010,277(1):174-185.
[18]	Itoh H, Komatsuda A, Ohtani H, et al. Mammalian HSP60 is quickly sorted into the mitochondria under conditions of dehydration[J].European Journal of Biochemistry.2002,269(23):5931-5938.
[19]	Cui Y D, Du Y Z, Lu M X, et al. Cloning of the heat shock protein 60 gene from the stem borer, Chilo suppressalis, and analysis of expression characteristics under heat stress[J].Journal of Insect Science.2010, 10(100):100.
[20]	Wang H, Lei Z, Li X, et al. Rapid cold hardening and expression of heat shock protein genes in the B-bio type Bemisia tabaci[J].Environmental Entomology.2011,40(1):132-139.
[21]	Rinehart J P, Denlinger D L. Heat-shock protein 90 is down-regulated during pupal diapause in the flesh fly, Sarcophaga crassipalpis, but remains responsive to thermal stress[J].Insect Molecular Biology.2000,9(6):641-645.
[22]	Shue G, Kohtz D S. Structural and functional aspects of basic helix-loop-helix protein folding byheat-shock protein 90[J].Journal of Biological Chemistry.1994,269(4):2707-2711.
[23]	Li Q R, Xiao Y, Wu F Q, et al. Analysis of midgut gene expression profiles from different silkworm varieties after exposure to high temperature[J].Gene.2014,549(1):85-96.

Sequence Analysis of Heat Shock Protein of Ericerus pela

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通讯作者: 陈斌, bchen63@163.com

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Sequence Analysis of Heat Shock Protein of Ericerus pela

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