Synthesis of Betulin in Suspension Cells of Birch Induced by Interactions of Sodium Nitroprusside (SNP) and K+ or Ca2+

HUANG Ya-ting; ZHAN Ya-guang; MA Ming-mei; FAN Gui-zhi

Volume 29 Issue 6

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Synthesis of Betulin in Suspension Cells of Birch Induced by Interactions of Sodium Nitroprusside (SNP) and K+ or Ca2+

1.
College of Life Sciences, Northeast Forestry University, Harbin 150040, Heilongjiang, China

Received Date: 2015-10-10

Abstract

[Objective] To clarify the effect of sodium nitroprusside (SNP) on content of K+ and Ca2+ and synthesis of betulin in suspension cells of birch and whether accumulation of betulin was induced by the interactions of SNP and K+ or Ca2+. [Methods] The content changes of betulin, K+ and Ca2+ were analyzed by HPLC, atomic absorption spectroscopy and chemical colorimetry, respectively. [Results] The contents of K+ and Ca2+ and synthesis of betulin were influenced by the treatment concentration and time of SNP. The highest contents of K+ and Ca2+ were 1.47 and 1.50 times that of the control after adding 1 mmol·L-1 SNP for 12 hours and 5 mmol·L-1 SNP for 96 hours, respectively. The synthesis of betulin after adding 1 mmol·L-1 SNP with different concentrations of KCl was higher than that treated by SNP and KCl alone. The highest content of betulin was 1.72 and 2.33 times that of the contents of SNP and KCl alone when treated by SNP with 0.1 g·L-1 KCl. The synthesis of betulin after adding 1 mmol·L-1 SNP with different concentrations of CaCl2 was higher than that treated by SNP and CaCl2 alone. The highest content of betulin was 2.75 and 2.18 times that of SNP and CaCl2 alone when treated by SNP with 0.1 g·L-1 CaCl2. [Conclusion] SNP promoted the contents of K+ and Ca2+ and synthesis of betulin in suspension cells of birch and the synthesis of betulin was better when treated by interactions of SNP and K+ or Ca2+. It is surmised that K+ and Ca2+ mediated the synthesis of betulin in suspension cells of birch induced by SNP.
- suspension cells of birch,
- Betulin,
- sodium nitroprusside (SNP),
- potassium,
- calcium

References

[1]	范桂枝, 詹亚光, 王博, 等. 激素配比对白桦悬浮细胞中三萜积累的影响[J]. 农业科学与技术, 2008, 2(12): 1-4.
[2]	尹静, 詹亚光, 李新宇, 等. 不同树龄白桦的不同器官及其组培苗诱导的愈伤组织中白桦酯醇和齐墩果酸的分布和含量变化[J]. 植物生理学通讯, 2009, (6): 610-614.
[3]	Zhou J, Huang L, Hachey D L, et al. Inhibition of HIV-1 maturation via drug association with the viral Gag protein in immature HIV-1 particles[J].Journal of Biological Chemistry, 2005, 280(51): 42149-42155.
[4]	Wen Z, Martin D E, Bullock P, et al. Glucuronidation of anti-HIV drug candidate bevirimat:identification of human UDP-glucuronosyltransferases and species differences[J]. Drug metabolism and disposition, 2007, 35(3): 440-448.
[5]	王博, 范桂枝, 詹亚光, 等. 不同碳源对白桦愈伤组织生长和三萜积累的影响[J]. 植物生理学通讯, 2008, 44(1): 97-99.
[6]	王博, 范桂枝, 詹亚光. 不同培养基类型和植物生长调节剂配比对白桦愈伤组织中三萜积累的影响[J]. 林业科学, 2008, 44(10): 153-158..
[7]	李晓灿, 詹亚光, 王晓东, 等. 多胺对白桦悬浮细胞生长和三萜积累的影响[J]. 中草药, 2013, 4: 463-467.
[8]	刘英甜, 王晓东, 周文洋, 等. 多胺介导真菌诱导子促进白桦三萜积累的初步研究[J]. 中草药, 2014, 45(5): 695-700
[9]	翟俏丽, 范桂枝, 詹亚光. 真菌诱导子促进白桦悬浮细胞三萜的积累[J]. 林业科学, 2011, 47(6): 42-47.
[10]	孙美玲, 李晓灿, 王晓东, 等. H₂O₂介导真菌诱导子促进白桦酯醇积累[J]. 林业科学, 2013, 49(7): 57-61.
[11]	马明媚, 詹亚光, 王晓东, 等. 腐胺与过氧化氢交互调控白桦三萜合成的初步研究[J]. 中草药, 2013, 44(20): 2916-2922.
[12]	王晓东. PAs, NO 和 H₂0₂ 在真菌促进白桦三萜合成中的对话研究. 东北林业大学, 2012.
[13]	Pedroso M C, Magalhaes J R, Durzan D. A nitric oxide burst precedes apoptosis in angiosperm and gymnosperm callus cells and foliar tissues[J]. Journal of Experimental Botany, 2000, 51(347): 1027-1036.
[14]	Klessig D F, Durner J, Noad R, et al. Nitric oxide and salicylic acid signaling in plant defense[J]. Proceedings of the National Academy of Sciences, 2000, 97(16): 8849-8855.
[15]	汪涛, 刘宇, 黄群山, 等. 一氧化氮对动物生殖机能的影响[J]. 动物医学进展, 2006, 27(2): 27-30.
[16]	王秀娟, 杨舒黎, 李帅, 等. 一氧化氮在动物低氧适应中的生理和分子机制[J]. 中国畜牧兽医, 2011, 38(5): 62-65.
[17]	徐茂军. 一氧化氮: 植物细胞次生代谢信号转导网络可能的关键节点[J]. 自然科学进展, 2008, 17(12): 1622-1630.
[18]	陈伟华, 鲍峰伟, 张晓静, 等. 微波消解-连续流动火焰光度法测定烤烟中的钾含量[J]. 分析测试技术与仪器, 2010, (2): 120-122.
[19]	YC/T217-2007.烟草及烟草制品钾的测定[S].
[20]	Castielli O, Cerda BD, Navarro JA, et al. Proteomic analyses of the response of cyanobacteria to different stress conditions. FEBS Lett, 2009, 583: 1753-1758.
[21]	Lee K, Bae DW, Kim SH, et al. Comparative proteomic analysis of the short-term responses of rice roots and leaves to cadmium. J Plant Physiol, 2010, 167: 161-168.
[22]	Zhang HX, Lian CL, Shen ZG. Proteomic identification of small, copper-responsive proteins in germinating embryos of Oryza sativa. Ann Bot, 2009, 103(6):923-930.
[23]	Modolo L V, Cunha F Q, Braga M R, et al. Nitric Oxide Synthase-Mediated Phytoalexin Accumulation in Soybean Cotyledons in Response to the Diaporthe phaseolorumf. sp. meridionalis Elicitor[J]. Plant Physiology, 2002, 130(3): 1288-1297.
[24]	徐茂军,董菊芳,朱睦元.NO参与真菌诱导子对红豆杉悬浮细胞中PAL活化和紫杉醇生物合成的促进作用[J].科学通报,2004,49(7):667-672.
[25]	Lamotte O, Courtois C, Dobrowolska G, et al. Mechanisms of nitric-oxide-induced increase of free cytosolic Ca²⁺ concentration in Nicotiana plumbaginifolia cells[J]. Free Radical Biology and Medicine, 2006, 40(8): 1369-1376.
[26]	Wang Y, Chen T, Zhang C, et al. Nitric oxide modulates the influx of extracellular Ca²⁺ and actin filament organization during cell wall construction in Pinus bungeana pollen tubes[J]. New Phytologist, 2009, 182(4): 851-862.
[27]	Garcia-Mata C, Gay R, Sokolovski S, et al. Nitric oxide regulates K+ and Cl-channels in guard cells through a subset of abscisic acid-evoked signaling pathways[J]. Proceedings of the National Academy of Sciences, 2003, 100(19): 11116-11121.
[28]	刘连成, 董娟娥, 张婧一, 等. Ca²⁺对丹参培养细胞中迷迭香酸合成及其相关酶活性的影响[J]. Chinese Journal of Biotechnology, 2012, 28(11): 1359-1369
[29]	余朝阁, 周娜, 李天来, 等. 钙对化学诱抗剂诱导番茄叶片酚类物质含量的影响[J]. 沈阳农业大学学报, 2014, 45(1): 6-10.
[30]	姬晓明. 钾肥对白桦三萜合成关键酶基因表达的影响. 东北林业大学, 2013.
[31]	薛绍武, 杨频, 何奕昆. 一氧化氮对拟南芥保卫细胞内向钾通道的作用[J]. 科学通报, 2008, 8: 009.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Synthesis of Betulin in Suspension Cells of Birch Induced by Interactions of Sodium Nitroprusside (SNP) and K+ or Ca2+

1. College of Life Sciences, Northeast Forestry University, Harbin 150040, Heilongjiang, China

Received Date: 2015-10-10

Abstract: [Objective] To clarify the effect of sodium nitroprusside (SNP) on content of K+ and Ca2+ and synthesis of betulin in suspension cells of birch and whether accumulation of betulin was induced by the interactions of SNP and K+ or Ca2+. [Methods] The content changes of betulin, K+ and Ca2+ were analyzed by HPLC, atomic absorption spectroscopy and chemical colorimetry, respectively. [Results] The contents of K+ and Ca2+ and synthesis of betulin were influenced by the treatment concentration and time of SNP. The highest contents of K+ and Ca2+ were 1.47 and 1.50 times that of the control after adding 1 mmol·L-1 SNP for 12 hours and 5 mmol·L-1 SNP for 96 hours, respectively. The synthesis of betulin after adding 1 mmol·L-1 SNP with different concentrations of KCl was higher than that treated by SNP and KCl alone. The highest content of betulin was 1.72 and 2.33 times that of the contents of SNP and KCl alone when treated by SNP with 0.1 g·L-1 KCl. The synthesis of betulin after adding 1 mmol·L-1 SNP with different concentrations of CaCl2 was higher than that treated by SNP and CaCl2 alone. The highest content of betulin was 2.75 and 2.18 times that of SNP and CaCl2 alone when treated by SNP with 0.1 g·L-1 CaCl2. [Conclusion] SNP promoted the contents of K+ and Ca2+ and synthesis of betulin in suspension cells of birch and the synthesis of betulin was better when treated by interactions of SNP and K+ or Ca2+. It is surmised that K+ and Ca2+ mediated the synthesis of betulin in suspension cells of birch induced by SNP.

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Reference (31)

[1]	范桂枝, 詹亚光, 王博, 等. 激素配比对白桦悬浮细胞中三萜积累的影响[J]. 农业科学与技术, 2008, 2(12): 1-4.
[2]	尹静, 詹亚光, 李新宇, 等. 不同树龄白桦的不同器官及其组培苗诱导的愈伤组织中白桦酯醇和齐墩果酸的分布和含量变化[J]. 植物生理学通讯, 2009, (6): 610-614.
[3]	Zhou J, Huang L, Hachey D L, et al. Inhibition of HIV-1 maturation via drug association with the viral Gag protein in immature HIV-1 particles[J].Journal of Biological Chemistry, 2005, 280(51): 42149-42155.
[4]	Wen Z, Martin D E, Bullock P, et al. Glucuronidation of anti-HIV drug candidate bevirimat:identification of human UDP-glucuronosyltransferases and species differences[J]. Drug metabolism and disposition, 2007, 35(3): 440-448.
[5]	王博, 范桂枝, 詹亚光, 等. 不同碳源对白桦愈伤组织生长和三萜积累的影响[J]. 植物生理学通讯, 2008, 44(1): 97-99.
[6]	王博, 范桂枝, 詹亚光. 不同培养基类型和植物生长调节剂配比对白桦愈伤组织中三萜积累的影响[J]. 林业科学, 2008, 44(10): 153-158..
[7]	李晓灿, 詹亚光, 王晓东, 等. 多胺对白桦悬浮细胞生长和三萜积累的影响[J]. 中草药, 2013, 4: 463-467.
[8]	刘英甜, 王晓东, 周文洋, 等. 多胺介导真菌诱导子促进白桦三萜积累的初步研究[J]. 中草药, 2014, 45(5): 695-700
[9]	翟俏丽, 范桂枝, 詹亚光. 真菌诱导子促进白桦悬浮细胞三萜的积累[J]. 林业科学, 2011, 47(6): 42-47.
[10]	孙美玲, 李晓灿, 王晓东, 等. H₂O₂介导真菌诱导子促进白桦酯醇积累[J]. 林业科学, 2013, 49(7): 57-61.
[11]	马明媚, 詹亚光, 王晓东, 等. 腐胺与过氧化氢交互调控白桦三萜合成的初步研究[J]. 中草药, 2013, 44(20): 2916-2922.
[12]	王晓东. PAs, NO 和 H₂0₂ 在真菌促进白桦三萜合成中的对话研究. 东北林业大学, 2012.
[13]	Pedroso M C, Magalhaes J R, Durzan D. A nitric oxide burst precedes apoptosis in angiosperm and gymnosperm callus cells and foliar tissues[J]. Journal of Experimental Botany, 2000, 51(347): 1027-1036.
[14]	Klessig D F, Durner J, Noad R, et al. Nitric oxide and salicylic acid signaling in plant defense[J]. Proceedings of the National Academy of Sciences, 2000, 97(16): 8849-8855.
[15]	汪涛, 刘宇, 黄群山, 等. 一氧化氮对动物生殖机能的影响[J]. 动物医学进展, 2006, 27(2): 27-30.
[16]	王秀娟, 杨舒黎, 李帅, 等. 一氧化氮在动物低氧适应中的生理和分子机制[J]. 中国畜牧兽医, 2011, 38(5): 62-65.
[17]	徐茂军. 一氧化氮: 植物细胞次生代谢信号转导网络可能的关键节点[J]. 自然科学进展, 2008, 17(12): 1622-1630.
[18]	陈伟华, 鲍峰伟, 张晓静, 等. 微波消解-连续流动火焰光度法测定烤烟中的钾含量[J]. 分析测试技术与仪器, 2010, (2): 120-122.
[19]	YC/T217-2007.烟草及烟草制品钾的测定[S].
[20]	Castielli O, Cerda BD, Navarro JA, et al. Proteomic analyses of the response of cyanobacteria to different stress conditions. FEBS Lett, 2009, 583: 1753-1758.
[21]	Lee K, Bae DW, Kim SH, et al. Comparative proteomic analysis of the short-term responses of rice roots and leaves to cadmium. J Plant Physiol, 2010, 167: 161-168.
[22]	Zhang HX, Lian CL, Shen ZG. Proteomic identification of small, copper-responsive proteins in germinating embryos of Oryza sativa. Ann Bot, 2009, 103(6):923-930.
[23]	Modolo L V, Cunha F Q, Braga M R, et al. Nitric Oxide Synthase-Mediated Phytoalexin Accumulation in Soybean Cotyledons in Response to the Diaporthe phaseolorumf. sp. meridionalis Elicitor[J]. Plant Physiology, 2002, 130(3): 1288-1297.
[24]	徐茂军,董菊芳,朱睦元.NO参与真菌诱导子对红豆杉悬浮细胞中PAL活化和紫杉醇生物合成的促进作用[J].科学通报,2004,49(7):667-672.
[25]	Lamotte O, Courtois C, Dobrowolska G, et al. Mechanisms of nitric-oxide-induced increase of free cytosolic Ca²⁺ concentration in Nicotiana plumbaginifolia cells[J]. Free Radical Biology and Medicine, 2006, 40(8): 1369-1376.
[26]	Wang Y, Chen T, Zhang C, et al. Nitric oxide modulates the influx of extracellular Ca²⁺ and actin filament organization during cell wall construction in Pinus bungeana pollen tubes[J]. New Phytologist, 2009, 182(4): 851-862.
[27]	Garcia-Mata C, Gay R, Sokolovski S, et al. Nitric oxide regulates K+ and Cl-channels in guard cells through a subset of abscisic acid-evoked signaling pathways[J]. Proceedings of the National Academy of Sciences, 2003, 100(19): 11116-11121.
[28]	刘连成, 董娟娥, 张婧一, 等. Ca²⁺对丹参培养细胞中迷迭香酸合成及其相关酶活性的影响[J]. Chinese Journal of Biotechnology, 2012, 28(11): 1359-1369
[29]	余朝阁, 周娜, 李天来, 等. 钙对化学诱抗剂诱导番茄叶片酚类物质含量的影响[J]. 沈阳农业大学学报, 2014, 45(1): 6-10.
[30]	姬晓明. 钾肥对白桦三萜合成关键酶基因表达的影响. 东北林业大学, 2013.
[31]	薛绍武, 杨频, 何奕昆. 一氧化氮对拟南芥保卫细胞内向钾通道的作用[J]. 科学通报, 2008, 8: 009.

Synthesis of Betulin in Suspension Cells of Birch Induced by Interactions of Sodium Nitroprusside (SNP) and K+ or Ca2+

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通讯作者: 陈斌, bchen63@163.com

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Synthesis of Betulin in Suspension Cells of Birch Induced by Interactions of Sodium Nitroprusside (SNP) and K+ or Ca2+

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