Genetic Diversity Analysis of Ancient Chestnut Trees Based on Fluorescent SSR Markers

LIU Guo-bin; CAO Jun; WANG Jin-bao; LAN Yan-ping

Volume 29 Issue 6

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Genetic Diversity Analysis of Ancient Chestnut Trees Based on Fluorescent SSR Markers

1.
Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences
2.
Beijing 100093, China

Received Date: 2015-12-04

Abstract

[Objective] The genetic variations of ancient chestnut trees in Ming and Qing dynasties were studied based on molecular markers. [Methods] 33 accessions were collected from Bohai town at Huairou district of Beijing, and the genetic diversity and polymorphism were detected by using 13 fluorescent polymorphic simple sequence repeats (SSRs). [Results] Thirteen SSR primers amplified 61 polymorphic alleles and 81 amplified bands in the 33 ancient chestnut trees, the number of alleles per locus ranged from 2 to 11 with an average of 4.7, and the amplified bands of 13 pairs of primers were 2 to 16 with an average of 6.2. The mean expected heterozygosity (HE) and polymorphic information content (PIC) were 0.4528 and 0.4103, respectively. PRA 75, PRA 83, PRD 25, CsCAT7 and PRD 53 can distinguish all of the ancient chestnut trees. [Conclusion] The habitats of chestnut trees in Ming and Qing dynasties were identical, the gene flow and gene exchange existed in a small area, which may be closely related to the low genetic diversity level. The study provides references for genetic diversity protection and utilization of chestnut germplasm resource. And the amplified bands and peak figures of 33 chestnut trees could lay the foundation for germplasm identification of the chestnut trees in Ming and Qing dynasties.
- ancient chestnut trees,
- fluorescent SSR markers,
- genetic diversity,
- germplasm identification

References

[1]	曹均. 全国板栗产业调查报告[M]. 北京:中国林业出版社, 2014, 1-10.
[2]	兰彦平,刘建玲,刘金海,等. 北京市怀柔区九渡河镇古板栗资源调查[J]. 经济林研究, 2013, 31(3): 161-164.
[3]	陈巍,王力荣,朱更瑞,等.基于SSR标记和生物学性状进行桃遗传多样性的比较分析[J].植物遗传资源学报,2009,10(1):86-90
[4]	曾淇,李明芳,郑学勤.基于SSR标记的荔枝种质遗传多样性分析[J].植物遗传资源学报,2010,11(3):298-304
[5]	薛杨,宋健坤,李鼎立,等.梨砧木种质资源的SSR遗传多样性分析[J].植物遗传资源学报,2013,14(6):1190-1195
[6]	韩柏明,赵密珍.基于SSR标记的草莓品种亲缘关系分析[J].植物遗传资源学报,2013,14(3):428-433
[7]	张成才,刘园,姜燕华,等.SSR标记鉴定浙江省主要无性系茶树品种的研究[J].植物遗传资源学报,2014,15(5);926-931
[8]	范建光,张海英,宫国义,等.西瓜DUS测试标准品种SSR指纹图谱构建及应用[J].植物遗传资源学报,2013,14(5):892-899
[9]	Buck EJ, Hadonou M, James CJ, et al. Isolation and characterization of polymorphic microsatellites in European chestnut (Castanea sativa Mill.) [J]. Molecular Ecology, 2003, 3, 239-241.
[10]	Marinoni D, Akkak A, Bounous G, et al. Development and characterization of microsatellite markers in Castanea sativa (Mill) [J]. Molecular Breeding, 2003, 11: 127-136.
[11]	Yamamoto T, Tahaka T, Kotobuki K, et al. Characterization of simple sequence repeats in Japanese chestnut[J]. Journal Horticultural Science and Biotechnology, 2003, 78, 197-203.
[12]	Tanaka T, Yamamoto T, Suzuki M. Genetic diversity of Castanea crenata in northern Japan assessed by SSR markers[J]. Breeding Science, 2005, 55, 271-277.
[13]	Nishio S, Yamamoto T, Terakami S, et al. Genetic diversity of Japanese chestnut cultivars assessed by SSR markers[J]. Breeding Science, 2011, 61:109-120.
[14]	王英,康明,黄宏文.用分子标记揭示植物随机大居群中亚居群的遗传结构—茅栗自然居群空间遗传结构的SSR分析[J]. 植物生态学报, 2006,30(1): 147-156
[15]	Wang Y, Kang M, Huang H W. Microsatellite loci transferability in chestnut[J]. Journal of the American Society for Horticultural Science, 2008, 133, 692-700.
[16]	田华,康明,李丽,等.中国板栗自然居群微卫星(SSR) 遗传多样性[J].生物多样性,2009,17(3): 296-302.
[17]	龚榜初,刘国彬. 锥栗自然居群遗传多样性的ISSR分析[J].植物遗传资源学报,2013,14(4):581-587.
[18]	郎萍,黄宏文. 栗属中国特有种居群的遗传多样性及地域差异[J].植物学报,1999,41(6):651-657.
[19]	艾呈祥,余贤美,张力思,等. 山东板栗遗传多样性分析[J].果树学报,2006,23:681-684.
[20]	兰彦平,周连第,姚研武,等.中国板栗种质资源的AFLP分析[J].园艺学报,2010,37(9):1499-1506.
[21]	刘国彬,龚榜初,赖俊声,等.锥栗农家品种的遗传多样性及亲缘关系分析[J].林业科学研究,2011,24(6):707-7012.
[22]	郝晨阳,王兰芬,贾继增,等. SSR荧光标记和银染技术的比较分析[J].作物学报,2005,31(2):144-149.
[23]	程本义,夏俊辉,龚俊义,等. SSR荧光标记毛细管电泳检测法在水稻DNA指纹鉴定中的应用[J].中国水稻科学,2011,25(6):672-676.
[24]	王凤格,田红丽,赵久然,等. 中国328个玉米品种(组合)SSR标记遗传多样性分析[J].中国农业科学,2014,47(5):856-864.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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Genetic Diversity Analysis of Ancient Chestnut Trees Based on Fluorescent SSR Markers

1. Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences

2. Beijing 100093, China

Received Date: 2015-12-04

Abstract: [Objective] The genetic variations of ancient chestnut trees in Ming and Qing dynasties were studied based on molecular markers. [Methods] 33 accessions were collected from Bohai town at Huairou district of Beijing, and the genetic diversity and polymorphism were detected by using 13 fluorescent polymorphic simple sequence repeats (SSRs). [Results] Thirteen SSR primers amplified 61 polymorphic alleles and 81 amplified bands in the 33 ancient chestnut trees, the number of alleles per locus ranged from 2 to 11 with an average of 4.7, and the amplified bands of 13 pairs of primers were 2 to 16 with an average of 6.2. The mean expected heterozygosity (HE) and polymorphic information content (PIC) were 0.4528 and 0.4103, respectively. PRA 75, PRA 83, PRD 25, CsCAT7 and PRD 53 can distinguish all of the ancient chestnut trees. [Conclusion] The habitats of chestnut trees in Ming and Qing dynasties were identical, the gene flow and gene exchange existed in a small area, which may be closely related to the low genetic diversity level. The study provides references for genetic diversity protection and utilization of chestnut germplasm resource. And the amplified bands and peak figures of 33 chestnut trees could lay the foundation for germplasm identification of the chestnut trees in Ming and Qing dynasties.

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Reference (24)

[1]	曹均. 全国板栗产业调查报告[M]. 北京:中国林业出版社, 2014, 1-10.
[2]	兰彦平,刘建玲,刘金海,等. 北京市怀柔区九渡河镇古板栗资源调查[J]. 经济林研究, 2013, 31(3): 161-164.
[3]	陈巍,王力荣,朱更瑞,等.基于SSR标记和生物学性状进行桃遗传多样性的比较分析[J].植物遗传资源学报,2009,10(1):86-90
[4]	曾淇,李明芳,郑学勤.基于SSR标记的荔枝种质遗传多样性分析[J].植物遗传资源学报,2010,11(3):298-304
[5]	薛杨,宋健坤,李鼎立,等.梨砧木种质资源的SSR遗传多样性分析[J].植物遗传资源学报,2013,14(6):1190-1195
[6]	韩柏明,赵密珍.基于SSR标记的草莓品种亲缘关系分析[J].植物遗传资源学报,2013,14(3):428-433
[7]	张成才,刘园,姜燕华,等.SSR标记鉴定浙江省主要无性系茶树品种的研究[J].植物遗传资源学报,2014,15(5);926-931
[8]	范建光,张海英,宫国义,等.西瓜DUS测试标准品种SSR指纹图谱构建及应用[J].植物遗传资源学报,2013,14(5):892-899
[9]	Buck EJ, Hadonou M, James CJ, et al. Isolation and characterization of polymorphic microsatellites in European chestnut (Castanea sativa Mill.) [J]. Molecular Ecology, 2003, 3, 239-241.
[10]	Marinoni D, Akkak A, Bounous G, et al. Development and characterization of microsatellite markers in Castanea sativa (Mill) [J]. Molecular Breeding, 2003, 11: 127-136.
[11]	Yamamoto T, Tahaka T, Kotobuki K, et al. Characterization of simple sequence repeats in Japanese chestnut[J]. Journal Horticultural Science and Biotechnology, 2003, 78, 197-203.
[12]	Tanaka T, Yamamoto T, Suzuki M. Genetic diversity of Castanea crenata in northern Japan assessed by SSR markers[J]. Breeding Science, 2005, 55, 271-277.
[13]	Nishio S, Yamamoto T, Terakami S, et al. Genetic diversity of Japanese chestnut cultivars assessed by SSR markers[J]. Breeding Science, 2011, 61:109-120.
[14]	王英,康明,黄宏文.用分子标记揭示植物随机大居群中亚居群的遗传结构—茅栗自然居群空间遗传结构的SSR分析[J]. 植物生态学报, 2006,30(1): 147-156
[15]	Wang Y, Kang M, Huang H W. Microsatellite loci transferability in chestnut[J]. Journal of the American Society for Horticultural Science, 2008, 133, 692-700.
[16]	田华,康明,李丽,等.中国板栗自然居群微卫星(SSR) 遗传多样性[J].生物多样性,2009,17(3): 296-302.
[17]	龚榜初,刘国彬. 锥栗自然居群遗传多样性的ISSR分析[J].植物遗传资源学报,2013,14(4):581-587.
[18]	郎萍,黄宏文. 栗属中国特有种居群的遗传多样性及地域差异[J].植物学报,1999,41(6):651-657.
[19]	艾呈祥,余贤美,张力思,等. 山东板栗遗传多样性分析[J].果树学报,2006,23:681-684.
[20]	兰彦平,周连第,姚研武,等.中国板栗种质资源的AFLP分析[J].园艺学报,2010,37(9):1499-1506.
[21]	刘国彬,龚榜初,赖俊声,等.锥栗农家品种的遗传多样性及亲缘关系分析[J].林业科学研究,2011,24(6):707-7012.
[22]	郝晨阳,王兰芬,贾继增,等. SSR荧光标记和银染技术的比较分析[J].作物学报,2005,31(2):144-149.
[23]	程本义,夏俊辉,龚俊义,等. SSR荧光标记毛细管电泳检测法在水稻DNA指纹鉴定中的应用[J].中国水稻科学,2011,25(6):672-676.
[24]	王凤格,田红丽,赵久然,等. 中国328个玉米品种(组合)SSR标记遗传多样性分析[J].中国农业科学,2014,47(5):856-864.

Genetic Diversity Analysis of Ancient Chestnut Trees Based on Fluorescent SSR Markers

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通讯作者: 陈斌, bchen63@163.com

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Genetic Diversity Analysis of Ancient Chestnut Trees Based on Fluorescent SSR Markers

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