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Citation:

Phenotypes Analysis for T-DNA Insertional Mutants of Paecilomyces lilacinus

  • Received Date: 2013-12-27
  • The T-DNA insertional mutants of Paecilomyces lilacinus were studied and the phenotypes were identified. It may contribute to understanding the pathogenic mechanism of P. lilacinus and the clone genes related to pathogenicity. All of 14 mutants generated by T-DNA insertion were tested with PCR for their insertional sequence. The morphology, colony growing rate, spore production, conidia germination rate, dry weight of mycelium and pathogenicity against Meloidogyne incongnita of phenotypes of mutants were analyzed by comparing with wild-type strain 20-7. The results demonstrated that the genomes of these mutants contained the sequence of beta-tubulin gene and T-DNA had been integrated into wild-type genome of P. lilacinus. Compared with wild-type strain 20-7, the morphologies of these colonies changed at different degrees. The colony growing rate of 85.71% strains speeded up obviously, only the strain BN-11 slowed down its colony growing rate distinctly. The strain BN-11 and BN-12, accounting for 14.29% of the total, increased the spore production. 78.57% mutants changed their conidia germination rate significantly. Both the rates of mutants that dry weight of mycelium reduced and increased were 21.43%. One mutant with increased pathogenicity was obtained, and the pathogenicity of 11 mutants decreased enormously.
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Phenotypes Analysis for T-DNA Insertional Mutants of Paecilomyces lilacinus

  • 1. Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry

Abstract: The T-DNA insertional mutants of Paecilomyces lilacinus were studied and the phenotypes were identified. It may contribute to understanding the pathogenic mechanism of P. lilacinus and the clone genes related to pathogenicity. All of 14 mutants generated by T-DNA insertion were tested with PCR for their insertional sequence. The morphology, colony growing rate, spore production, conidia germination rate, dry weight of mycelium and pathogenicity against Meloidogyne incongnita of phenotypes of mutants were analyzed by comparing with wild-type strain 20-7. The results demonstrated that the genomes of these mutants contained the sequence of beta-tubulin gene and T-DNA had been integrated into wild-type genome of P. lilacinus. Compared with wild-type strain 20-7, the morphologies of these colonies changed at different degrees. The colony growing rate of 85.71% strains speeded up obviously, only the strain BN-11 slowed down its colony growing rate distinctly. The strain BN-11 and BN-12, accounting for 14.29% of the total, increased the spore production. 78.57% mutants changed their conidia germination rate significantly. Both the rates of mutants that dry weight of mycelium reduced and increased were 21.43%. One mutant with increased pathogenicity was obtained, and the pathogenicity of 11 mutants decreased enormously.

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